Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0002132

Physical Location & Seq

Genomic Coordinates Reverse (-)

30505388 .. 30508297

Gene Structure 2910 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G316200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGTGTGTGTGTGTGTGTGTGTTAGAGATAATTGATCTTCGTCCAGTGCAACGTTACTTACTGTCTTCATCAATGGCTAATTCTGATGTGTCAATGGTTTTGCCTAGGGTTCTTATCGTCTCTCGACGAACCCTCCGCAAGAACAAGTTTGTAGATTTCGTGGGAGAATATCATCTAGATCTAATTGTAAGCTATGGAGCAGTACCTGTTATTGTCCCCAGAGTCAGTGGAGTCCACATGCTACTCCAAAGCTTTGAACCAATACATGGAGTTCTTCTCTGCGAAGGTGAAGACATTGATCCGTCCCATTACGATGCGCAGCTCTCTGGTTTCTCTCCAGACGAGCTAGAAGAAATTGGCAAGCTACATGCAAGCGATACCGCCATTGATAAAGAGAAAGACTCCATTGAACTGGGGCTTGCAAAGCTATGCCTAGAAAGAAACATCCCCTACTTGGGAATATGCAGAGGATCCCAAGTCCTTAATGTTGCATGCGGAGGCACACTTTATCAAGATGTTGAGAGAGAACTTTTGAAAGAATGCCCATATCAGAGTTGCGCTCAAAGAGTGGCTCACATAAACTATGAGAATTATGATGGGCATAGACATGGGGTGAAGGTGGTGGAGAATACACCTTTGCATCAGTGGTTCAAAGATTCTTTGGATGATGAGAAAATGGAGATGTGGGTGAATAGTTATCACCATCAGGGGGTTAAGCGATTGGCAGAGCGATTCGCGCCGATGGCTTTCGCTCCTGATGGTTTGGTTGAAGGGTTTTATGATCCGGATGCCTATAATCCTGAGGAGGGGAAGTTTATTATGGGACTTCAATTCCATCCGGAGAGAATGCGACAACAGGATTCAGACAATTTTGAATATCCAGGATGCGCAGCTGCATATCGGGAATTTGTTAAAGCAGTGAGAGCTTATGAAAAGAAGCTCAGTCAAAACTCGGCATGTGTGCCGAAGGCTCCACAGCTTGATCAGGACCTAGAGATGAAGAGGAATAGTATTGTAAGGAGCTTCTCCATTGCCAAAAACATGTATTCTAGGCGTGGAATTGTTTCAGGCAAAGAATCTGATCAACTGGACGTTGGAGCAGAGTTTCTGGAGGCAAATACAGCACTGAGTTTGCAGCAAGAGAAAAGGCTGAAGCAGATGGGGGCAACAGTAAGAAATGCTTCGGCATACATACAACGACTGAAGATGAGCGAGGAAAGGGAGAGAGTTGCAAGGGCCATAATTGGGAAGATGTCGATAGAGCAGCTTTCTGATTTGTTGTCTTTCTACCGCATTATGAGTCAGATGTGTTCCGACGCTTTGGACACCAAGTTCCATGACCTGCTTAATAATGAAGTTGAAGTTGATGGATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0022603 GO:0048831 GO:0050789 GO:0050793 GO:0051239 GO:0060688 GO:0065007 GO:1900618 GO:1905428 GO:2000026 GO:2000032

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C26

Protein Analysis

459

Amino Acids

51.92

Weight (kDa)

5.49

Isoelectric Point (pI)

48.75

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66860 AT5G38200 AT5G38200 AT5G38200
fragaria_vesca	FvH4_1g15830 FvH4_1g15830 FvH4_1g15830 FvH4_1g15830 FvH4_4g26770
malus_domestica	MD09G1255400.v1.1 MD16G1037600.v1.1 MD17G1248500.v1.1
prunus_persica	Prupe.1G316200_v2.0.a1 Prupe.3G147100_v2.0.a1
pyrus_communis	pycom09g17120 pycom17g25160 pycom17g25200
rosa_chinensis	RchiOBHm_Chr2g0105131 RchiOBHm_Chr4g0434451 RchiOBHm_Chr4g0434461
rosa_laevigata	RLG00000006670 RLG00000017405
rosa_multiflora	Rmu_sc0001170.1_g000001 Rmu_sc0002251.1_g000018 Rmu_sc0002251.1_g000020 Rmu_sc0005899.1_g000007 Rmu_sc0006734.1_g000002
rosa_roxburghii	Rroxscaffold_2G00138400 Rroxscaffold_5G00375810
rosa_rugosa	Rorug02G0126400 Rorug02G0126500 Rorug02G0126600 Rorug04G0276800 Rorug04G0276900
rosa_samantha	Rh2AG177300 Rh2BG185300 Rh2CG181800 Rh2DG183400 Rh4AG332400 Rh4BG340200 Rh4CG355400 Rh4DG335600
rosa_wichuraiana	Rw2G013910 Rw4G028820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	318, 889
Acc36I	ACCTGC	1 cut(s)	1350
AccB7I	CCANNNNNTGG	1 cut(s)	266
AccII	CGCG	1 cut(s)	737
AccIII	TCCGGA	2 cut(s)	784, 838
AciI	CCGC	4 cut(s)	136, 381, 495, 1291
AclI	AACGTT	1 cut(s)	52
AclWI	GGATC	4 cut(s)	293, 465, 478, 776
AcsI	RAATTY	1 cut(s)	905
AcuI	CTGAAG	2 cut(s)	1172, 1223
AfaI	GTAC	1 cut(s)	204
AfiI	CCNNNNNNNGG	6 cut(s)	266, 454, 455, 709, 806, 1245
AflIII	ACRYGT	1 cut(s)	1041
AgsI	TTSAA	8 cut(s)	257, 410, 535, 652, 770, 830, 875, 1361
AjnI	CCWGG	1 cut(s)	880
AloI	GAACNNNNNNTCC	2 cut(s)	1316, 1348
Alw26I	GTCTC	1 cut(s)	124
AlwI	GGATC	4 cut(s)	293, 465, 478, 776
AlwNI	CAGNNNCTG	1 cut(s)	206
Aor13HI	TCCGGA	2 cut(s)	784, 838
AoxI	GGCC	1 cut(s)	1236
ApeKI	GCWGC	5 cut(s)	319, 890, 893, 1135, 1264
ApoI	RAATTY	1 cut(s)	905
Asp700I	GAANNNNTTC	2 cut(s)	1063, 1180
AspA2I	CCTAGG	1 cut(s)	104
AspLEI	GCGC	4 cut(s)	319, 560, 739, 890
AspS9I	GGNCC	2 cut(s)	988, 1236
AsuHPI	GGTGA	4 cut(s)	299, 625, 692, 700
AvaII	GGWCC	1 cut(s)	988
AvrII	CCTAGG	1 cut(s)	104
AxyI	CCTNAGG	1 cut(s)	801
BamHI	GGATCC	1 cut(s)	470
BbsI	GAAGAC	2 cut(s)	57, 297
BbvI	GCAGC	5 cut(s)	331, 880, 902, 1147, 1276
BccI	CCATC	7 cut(s)	590, 711, 736, 752, 843, 1153, 1361
BciT130I	CCWGG	1 cut(s)	882
BclI	TGATCA	2 cut(s)	982, 1081
BcoDI	GTCTC	1 cut(s)	124
BfaI	CTAG	6 cut(s)	105, 176, 347, 434, 992, 1050
BfuAI	ACCTGC	1 cut(s)	1350
BglII	AGATCT	1 cut(s)	178
BisI	GCNGC	5 cut(s)	320, 891, 894, 1136, 1265
BlnI	CCTAGG	1 cut(s)	104
BlsI	GCNGC	5 cut(s)	321, 892, 895, 1137, 1266
Bme1390I	CCNGG	1 cut(s)	882
Bme18I	GGWCC	1 cut(s)	988
BmgT120I	GGNCC	2 cut(s)	988, 1236
BmiI	GGNNCC	2 cut(s)	472, 972
BmrFI	CCNGG	1 cut(s)	882
BmrI	ACTGGG	1 cut(s)	422
BmsI	GCATC	4 cut(s)	304, 649, 778, 875
BmuI	ACTGGG	1 cut(s)	422
BpiI	GAAGAC	2 cut(s)	57, 297
BplI	GAGNNNNNCTC	6 cut(s)	261, 293, 386, 418, 544, 576
BpmI	CTGGAG	2 cut(s)	321, 1130
BsaJI	CCNNGG	1 cut(s)	104
BsaWI	WCCGGW	2 cut(s)	784, 838
BsaXI	ACNNNNNCTCC	4 cut(s)	117, 147, 797, 827
Bsc4I	CCNNNNNNNGG	6 cut(s)	266, 454, 455, 709, 806, 1245
Bse1I	ACTGG	3 cut(s)	44, 417, 1092
Bse21I	CCTNAGG	1 cut(s)	801
Bse3DI	GCAATG	1 cut(s)	1029
BseAI	TCCGGA	2 cut(s)	784, 838
BseBI	CCWGG	1 cut(s)	882
BseDI	CCNNGG	1 cut(s)	104
BseGI	GGATG	5 cut(s)	444, 670, 793, 835, 890
BseLI	CCNNNNNNNGG	6 cut(s)	266, 454, 455, 709, 806, 1245
BseMI	GCAATG	1 cut(s)	1029
BseMII	CTCAG	3 cut(s)	792, 955, 1118
BseNI	ACTGG	3 cut(s)	44, 417, 1092
BseRI	GAGGAG	1 cut(s)	818
BseXI	GCAGC	5 cut(s)	331, 880, 902, 1147, 1276
Bsh1236I	CGCG	1 cut(s)	737
BshFI	GGCC	1 cut(s)	1238
BsiSI	CCGG	2 cut(s)	785, 839
BslFI	GGGAC	3 cut(s)	200, 289, 837
BslI	CCNNNNNNNGG	6 cut(s)	266, 454, 455, 709, 806, 1245
BsmAI	GTCTC	1 cut(s)	124
BsmBI	CGTCTC	1 cut(s)	124
BsmFI	GGGAC	3 cut(s)	200, 289, 837
BsmI	GAATGC	2 cut(s)	545, 852
BsnI	GGCC	1 cut(s)	1238
Bsp13I	TCCGGA	2 cut(s)	784, 838
Bsp143I	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
BspACI	CCGC	4 cut(s)	136, 381, 495, 1291
BspANI	GGCC	1 cut(s)	1238
BspCNI	CTCAG	3 cut(s)	793, 954, 1119
BspEI	TCCGGA	2 cut(s)	784, 838
BspFNI	CGCG	1 cut(s)	737
BspLI	GGNNCC	2 cut(s)	472, 972
BspMI	ACCTGC	1 cut(s)	1350
BspPI	GGATC	4 cut(s)	293, 465, 478, 776
BsrDI	GCAATG	1 cut(s)	1029
BsrI	ACTGG	3 cut(s)	44, 417, 1092
BssECI	CCNNGG	1 cut(s)	104
BssMI	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
BssT1I	CCWWGG	1 cut(s)	104
Bst2UI	CCWGG	1 cut(s)	882
Bst4CI	ACNGT	2 cut(s)	63, 1171
Bst6I	CTCTTC	1 cut(s)	995
BstC8I	GCNNGC	4 cut(s)	362, 373, 420, 493
BstDEI	CTNAG	3 cut(s)	801, 941, 1127
BstF5I	GGATG	5 cut(s)	444, 670, 793, 835, 890
BstFNI	CGCG	1 cut(s)	737
BstHHI	GCGC	4 cut(s)	319, 560, 739, 890
BstKTI	GATC	8 cut(s)	37, 181, 301, 473, 784, 985, 1084, 1373
BstMAI	GTCTC	1 cut(s)	124
BstMBI	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
BstMWI	GCNNNNNNNGC	5 cut(s)	424, 736, 743, 923, 1121
BstNI	CCWGG	1 cut(s)	882
BstNSI	RCATGY	5 cut(s)	241, 371, 495, 960, 1045
BstSCI	CCNGG	1 cut(s)	880
BstUI	CGCG	1 cut(s)	737
BstV1I	GCAGC	5 cut(s)	331, 880, 902, 1147, 1276
BstV2I	GAAGAC	2 cut(s)	57, 297
BstX2I	RGATCY	3 cut(s)	178, 470, 1370
BstXI	CCANNNNNNTGG	1 cut(s)	412
BstYI	RGATCY	3 cut(s)	178, 470, 1370
Bsu36I	CCTNAGG	1 cut(s)	801
BsuRI	GGCC	1 cut(s)	1238
BtsCI	GGATG	5 cut(s)	444, 670, 793, 835, 890
BtsI	GCAGTG	1 cut(s)	924
BtsIMutI	CAGTG	5 cut(s)	51, 232, 650, 924, 1124
BveI	ACCTGC	1 cut(s)	1350
Cac8I	GCNNGC	4 cut(s)	362, 373, 420, 493
CaiI	CAGNNNCTG	1 cut(s)	206
CfoI	GCGC	4 cut(s)	319, 560, 739, 890
Cfr13I	GGNCC	2 cut(s)	988, 1236
CseI	GACGC	1 cut(s)	1325
Csp6I	GTAC	1 cut(s)	203
CviAII	CATG	8 cut(s)	238, 266, 368, 492, 608, 957, 1042, 1337
CviQI	GTAC	1 cut(s)	203
DdeI	CTNAG	3 cut(s)	801, 941, 1127
DpnI	GATC	8 cut(s)	36, 180, 300, 472, 783, 984, 1083, 1372
DpnII	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
Eam1104I	CTCTTC	1 cut(s)	995
EarI	CTCTTC	1 cut(s)	995
Eco130I	CCWWGG	1 cut(s)	104
Eco47I	GGWCC	1 cut(s)	988
Eco57I	CTGAAG	2 cut(s)	1172, 1223
Eco81I	CCTNAGG	1 cut(s)	801
EcoO109I	RGGNCCY	1 cut(s)	988
EcoRII	CCWGG	1 cut(s)	880
EcoT14I	CCWWGG	1 cut(s)	104
ErhI	CCWWGG	1 cut(s)	104
Esp3I	CGTCTC	1 cut(s)	124
FaeI	CATG	8 cut(s)	241, 269, 371, 495, 611, 960, 1045, 1340
FalI	AAGNNNNNCTT	2 cut(s)	1165, 1197
FaqI	GGGAC	3 cut(s)	200, 289, 837
FatI	CATG	8 cut(s)	237, 265, 367, 491, 607, 956, 1041, 1336
FbaI	TGATCA	2 cut(s)	982, 1081
Fnu4HI	GCNGC	5 cut(s)	320, 891, 894, 1136, 1265
FokI	GGATG	5 cut(s)	431, 677, 800, 822, 897
Fsp4HI	GCNGC	5 cut(s)	320, 891, 894, 1136, 1265
FspBI	CTAG	6 cut(s)	105, 176, 347, 434, 992, 1050
FspI	TGCGCA	2 cut(s)	318, 889
GlaI	GCGC	4 cut(s)	318, 559, 738, 889
GluI	GCNGC	5 cut(s)	320, 891, 894, 1136, 1265
GsuI	CTGGAG	2 cut(s)	321, 1130
HaeIII	GGCC	1 cut(s)	1238
HapII	CCGG	2 cut(s)	785, 839
HgaI	GACGC	1 cut(s)	1325
HhaI	GCGC	4 cut(s)	319, 560, 739, 890
Hin1II	CATG	8 cut(s)	241, 269, 371, 495, 611, 960, 1045, 1340
Hin6I	GCGC	4 cut(s)	317, 558, 737, 888
HinP1I	GCGC	4 cut(s)	317, 558, 737, 888
HindIII	AAGCTT	1 cut(s)	250
HinfI	GANTC	8 cut(s)	222, 231, 401, 656, 732, 860, 1076, 1300
HpaII	CCGG	2 cut(s)	785, 839
HphI	GGTGA	4 cut(s)	299, 625, 692, 700
Hpy166II	GTNNAC	1 cut(s)	235
Hpy188I	TCNGA	7 cut(s)	85, 552, 865, 1081, 1273, 1305, 1315
Hpy8I	GTNNAC	1 cut(s)	235
Hpy99I	CGWCG	2 cut(s)	129, 1319
HpyAV	CCTTC	4 cut(s)	278, 610, 764, 961
HpyCH4III	ACNGT	2 cut(s)	63, 1171
HpyCH4IV	ACGT	2 cut(s)	52, 1092
HpyCH4V	TGCA	9 cut(s)	49, 371, 422, 465, 491, 640, 896, 1135, 1232
HpyF10VI	GCNNNNNNNGC	5 cut(s)	424, 736, 743, 923, 1121
HpyF3I	CTNAG	3 cut(s)	801, 941, 1127
HpySE526I	ACGT	2 cut(s)	52, 1092
Hsp92II	CATG	8 cut(s)	241, 269, 371, 495, 611, 960, 1045, 1340
HspAI	GCGC	4 cut(s)	317, 558, 737, 888
Kpn2I	TCCGGA	2 cut(s)	784, 838
Ksp22I	TGATCA	2 cut(s)	982, 1081
Kzo9I	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
LmnI	GCTCC	5 cut(s)	197, 757, 976, 1020, 1097
Lsp1109I	GCAGC	5 cut(s)	331, 880, 902, 1147, 1276
LweI	GCATC	4 cut(s)	304, 649, 778, 875
MaeI	CTAG	6 cut(s)	105, 176, 347, 434, 992, 1050
MaeII	ACGT	2 cut(s)	52, 1092
MaeIII	GTNAC	1 cut(s)	53
MalI	GATC	8 cut(s)	36, 180, 300, 472, 783, 984, 1083, 1372
MboI	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
MboII	GAAGA	8 cut(s)	29, 57, 266, 302, 362, 1012, 1216, 1261
MflI	RGATCY	3 cut(s)	178, 470, 1370
MlyI	GAGTC	4 cut(s)	231, 240, 395, 1309
MmeI	TCCRAC	2 cut(s)	1075, 1338
MnlI	CCTC	8 cut(s)	143, 461, 491, 796, 799, 996, 1105, 1207
MroI	TCCGGA	2 cut(s)	784, 838
MroXI	GAANNNNTTC	2 cut(s)	1063, 1180
MseI	TTAA	4 cut(s)	483, 714, 912, 1347
MslI	CAYNNNNRTG	2 cut(s)	312, 606
MspA1I	CMGCKG	1 cut(s)	893
MspI	CCGG	2 cut(s)	785, 839
MspR9I	CCNGG	1 cut(s)	882
Mva1269I	GAATGC	2 cut(s)	545, 852
MvaI	CCWGG	1 cut(s)	882
MvnI	CGCG	1 cut(s)	737
MwoI	GCNNNNNNNGC	5 cut(s)	424, 736, 743, 923, 1121
NdeII	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
NlaIII	CATG	8 cut(s)	241, 269, 371, 495, 611, 960, 1045, 1340
NlaIV	GGNNCC	2 cut(s)	472, 972
NmeAIII	GCCGAG	1 cut(s)	932
NsbI	TGCGCA	2 cut(s)	318, 889
NspI	RCATGY	5 cut(s)	241, 371, 495, 960, 1045
PaeI	GCATGC	1 cut(s)	495
PciI	ACATGT	1 cut(s)	1041
PctI	GAATGC	2 cut(s)	545, 852
PdmI	GAANNNNTTC	2 cut(s)	1063, 1180
PfeI	GAWTC	4 cut(s)	656, 732, 860, 1076
PflMI	CCANNNNNTGG	1 cut(s)	266
PfoI	TCCNGGA	1 cut(s)	880
PkrI	GCNGC	5 cut(s)	321, 892, 895, 1137, 1266
PleI	GAGTC	4 cut(s)	230, 239, 395, 1308
PpsI	GAGTC	4 cut(s)	230, 239, 395, 1308
PpuMI	RGGWCCY	1 cut(s)	988
PscI	ACATGT	1 cut(s)	1041
Psp1406I	AACGTT	1 cut(s)	52
Psp5II	RGGWCCY	1 cut(s)	988
Psp6I	CCWGG	1 cut(s)	880
PspGI	CCWGG	1 cut(s)	880
PspN4I	GGNNCC	2 cut(s)	472, 972
PspPI	GGNCC	2 cut(s)	988, 1236
PspPPI	RGGWCCY	1 cut(s)	988
PstNI	CAGNNNCTG	1 cut(s)	206
PsuI	RGATCY	3 cut(s)	178, 470, 1370
PvuII	CAGCTG	1 cut(s)	893
RsaI	GTAC	1 cut(s)	204
RsaNI	GTAC	1 cut(s)	203
RseI	CAYNNNNRTG	2 cut(s)	312, 606
SaqAI	TTAA	4 cut(s)	483, 714, 912, 1347
SatI	GCNGC	5 cut(s)	320, 891, 894, 1136, 1265
Sau3AI	GATC	8 cut(s)	34, 178, 298, 470, 781, 982, 1081, 1370
Sau96I	GGNCC	2 cut(s)	988, 1236
SchI	GAGTC	4 cut(s)	231, 240, 395, 1309
ScrFI	CCNGG	1 cut(s)	882
SfaNI	GCATC	4 cut(s)	304, 649, 778, 875
SinI	GGWCC	1 cut(s)	988
SmiMI	CAYNNNNRTG	2 cut(s)	312, 606
SphI	GCATGC	1 cut(s)	495
SsiI	CCGC	4 cut(s)	136, 381, 495, 1291
SspMI	CTAG	6 cut(s)	105, 176, 347, 434, 992, 1050
StyD4I	CCNGG	1 cut(s)	880
StyI	CCWWGG	1 cut(s)	104
TaaI	ACNGT	2 cut(s)	63, 1171
TaiI	ACGT	2 cut(s)	55, 1095
TaqI	TCGA	2 cut(s)	124, 1256
TfiI	GAWTC	4 cut(s)	656, 732, 860, 1076
Tru1I	TTAA	4 cut(s)	483, 714, 912, 1347
Tru9I	TTAA	4 cut(s)	483, 714, 912, 1347
TscAI	CASTG	5 cut(s)	51, 232, 650, 924, 1131
TseI	GCWGC	5 cut(s)	319, 890, 893, 1135, 1264
TspDTI	ATGAA	4 cut(s)	57, 945, 1013, 1368
TspGWI	ACGGA	1 cut(s)	291
TspRI	CASTG	5 cut(s)	51, 232, 650, 924, 1131
Van91I	CCANNNNNTGG	1 cut(s)	266
VpaK11BI	GGWCC	1 cut(s)	988
XapI	RAATTY	1 cut(s)	905
XbaI	TCTAGA	1 cut(s)	175
XceI	RCATGY	5 cut(s)	241, 371, 495, 960, 1045
XmaJI	CCTAGG	1 cut(s)	104
XmnI	GAANNNNTTC	2 cut(s)	1063, 1180
XspI	CTAG	6 cut(s)	105, 176, 347, 434, 992, 1050

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.