RchiOBHm_Chr4g0434451

Peptidase C26

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002132
Physical Location & Seq
Forward (+)
58224422 .. 58225798
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40290

Sequence Viewer

Length: 1026 bp
ATGGCTAATTCAGATCTCTCAATGGTTCTGCCTAGGGTTCTCATCGTCTCCCGCCGCACGCTCCGCAAGAACAAGTTTGTAGATTTCGTCGGAGAATATCATCTAGATCTAATTGTGAGCTATGGAGCGGTGCCTATTATTGTTCCACGAGTCAGTGGAGTCCATACACTACTCCAAAGCTTTGAACCAATCCATGGGGTTCTCCTATGCGAAGGGGAAGACATAGATCCATCACATTACGACGCAGAGCTCTCGGGGTTCTCCTCCGAGGAGCTCCAAGAAATCGGGAAGCTACACGCGAGTGACACCGTCATTGACAAAGAGAAAGACTCCATTGAACTGAGGCTGGCAAAGCTATGCCTAGAGAGGAACATACCCTACTTGGGAATATGCCGAGGGTCACAAGTCCTCAATGTGGCATGTGGGGGTACATTGTATCAGGATGTTGAGAAGGAACTCGAGAAAGAGTGCCCGGATCAGAATCAGAGAGTGACTCACATGAACTATGATGATTACGATGGACATAGACATGTGGTGAAGGTGGTGGAGAATACTCCTTTGCATCAATGGTTTAGAGATTCTTTGGATGATGAGAAAATGGAGATGAGAGTCAACAGTTATCACCATCAAGGTGTTAAGAGATTGGCGCAGAGGTTTGTGCCAATGGCTTTCGCCCCTGATGGTTTGATCGAAGGGTTTTATGATCCGGATGCTTATAATCCTGAAGAAGGTAAGTTCATCATGGGACTTCAATTTCATCCAGAGAGAATGCGGCAACTTGATTCGGACGATTTTGAGTACCCAGGATGCGCATATGCATATCAGGAATTTGTTAAAGCAGTAAGAGCTTATGAAAAGAAGCTCAGTCAGTCGGAGTGCGTGCCAAAGGCTCCAAAGCTTAACCATGAGCTAGAGATGAAGAGAAAGAGCCTCCTAAGGAGCTTCTCAGTCGCCAAAACATGTATTCTAGGCGTGGTAGTATATCAAGCAAAGAATCTGAACTGGAAGTTGGAGCTGAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0022603 GO:0048831 GO:0050789 GO:0050793 GO:0051239 GO:0060688 GO:0065007 GO:1900618 GO:1905428 GO:2000026 GO:2000032
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C26

Protein Analysis

341

Amino Acids

39.13

Weight (kDa)

5.66

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C26 PF07722 29 - 255 7.1e-42 Peptidase C26
GATase PF00117 113 - 257 3.7e-12 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
Acc16I TGCGCA 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 130
AccB7I CCANNNNNTGG 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 128
AccII CGCG 1 cut(s) 299
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 5 cut(s) 52, 55, 64, 128, 772
AclWI GGATC 3 cut(s) 221, 483, 698
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 2 cut(s) 430, 800
AfiI CCNNNNNNNGG 4 cut(s) 194, 383, 415, 728
AflIII ACRYGT 2 cut(s) 529, 959
AgsI TTSAA 3 cut(s) 185, 338, 752
AjnI CCWGG 1 cut(s) 802
AjuI GAANNNNNNNTTGG 2 cut(s) 992, 1024
AleI CACNNNNGTG 1 cut(s) 300
Alw21I GWGCWC 2 cut(s) 252, 276
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 3 cut(s) 221, 483, 698
Ama87I CYCGRG 2 cut(s) 253, 458
Aor13HI TCCGGA 1 cut(s) 706
ApoI RAATTY 1 cut(s) 827
AspA2I CCTAGG 1 cut(s) 32
AspLEI GCGC 2 cut(s) 649, 812
AsuC2I CCSGG 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 547, 614
AvaI CYCGRG 2 cut(s) 253, 458
AvrII CCTAGG 1 cut(s) 32
AxyI CCTNAGG 1 cut(s) 935
BaeGI GKGCMC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 252, 276
BauI CACGAG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 252, 276
BccI CCATC 4 cut(s) 238, 512, 633, 674
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 804
BcnI CCSGG 1 cut(s) 473
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 6 cut(s) 33, 104, 362, 911, 968, 1024
BglII AGATCT 2 cut(s) 13, 106
BisI GCNGC 2 cut(s) 55, 773
BlnI CCTAGG 1 cut(s) 32
BlsI GCNGC 2 cut(s) 56, 774
Bme1390I CCNGG 2 cut(s) 473, 804
BmeT110I CYCGRG 2 cut(s) 253, 458
BmiI GGNNCC 2 cut(s) 132, 891
BmrFI CCNGG 2 cut(s) 473, 804
BmsI GCATC 3 cut(s) 571, 700, 797
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 4 cut(s) 314, 346, 478, 510
BpuMI CCSGG 1 cut(s) 473
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 5 cut(s) 32, 193, 267, 394, 802
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 4 cut(s) 194, 383, 415, 728
Bse1I ACTGG 1 cut(s) 1007
Bse21I CCTNAGG 1 cut(s) 935
Bse8I GATNNNNATC 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 5 cut(s) 32, 193, 267, 394, 802
BseGI GGATG 5 cut(s) 448, 592, 715, 757, 812
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 4 cut(s) 194, 383, 415, 728
BseMII CTCAG 4 cut(s) 332, 877, 960, 1007
BseNI ACTGG 1 cut(s) 1007
BseRI GAGGAG 2 cut(s) 253, 284
BseSI GKGCMC 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 252, 276
BsiHKCI CYCGRG 2 cut(s) 253, 458
BsiSI CCGG 2 cut(s) 473, 707
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 4 cut(s) 194, 383, 415, 728
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 774
BsoBI CYCGRG 2 cut(s) 253, 458
Bsp1286I GDGCHC 3 cut(s) 252, 276, 473
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 6 cut(s) 13, 106, 226, 475, 687, 703
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 5 cut(s) 52, 55, 64, 128, 772
BspCNI CTCAG 4 cut(s) 333, 876, 959, 1008
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 2 cut(s) 132, 891
BspPI GGATC 3 cut(s) 221, 483, 698
BspT107I GGYRCC 1 cut(s) 130
BsrBI CCGCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 1007
BssECI CCNNGG 5 cut(s) 32, 193, 267, 394, 802
BssMI GATC 6 cut(s) 13, 106, 226, 475, 687, 703
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 32, 193
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 2 cut(s) 310, 617
Bst6I CTCTTC 1 cut(s) 914
BstC8I GCNNGC 3 cut(s) 59, 348, 881
BstDEI CTNAG 5 cut(s) 341, 863, 935, 946, 1016
BstDSI CCRYGG 1 cut(s) 193
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 5 cut(s) 448, 592, 715, 757, 812
BstFNI CGCG 1 cut(s) 299
BstHHI GCGC 2 cut(s) 649, 812
BstKTI GATC 6 cut(s) 16, 109, 229, 478, 690, 706
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 6 cut(s) 13, 106, 226, 475, 687, 703
BstMWI GCNNNNNNNGC 3 cut(s) 63, 352, 845
BstNI CCWGG 1 cut(s) 804
BstNSI RCATGY 3 cut(s) 423, 533, 963
BstSCI CCNGG 2 cut(s) 471, 802
BstSLI GKGCMC 1 cut(s) 473
BstUI CGCG 1 cut(s) 299
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 3 cut(s) 13, 106, 226
BstYI RGATCY 3 cut(s) 13, 106, 226
Bsu36I CCTNAGG 1 cut(s) 935
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 5 cut(s) 448, 592, 715, 757, 812
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 59, 348, 881
CfoI GCGC 2 cut(s) 649, 812
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 429, 799
CviAII CATG 7 cut(s) 194, 420, 499, 530, 742, 905, 960
CviQI GTAC 2 cut(s) 429, 799
DdeI CTNAG 5 cut(s) 341, 863, 935, 946, 1016
DpnI GATC 6 cut(s) 15, 108, 228, 477, 689, 705
DpnII GATC 6 cut(s) 13, 106, 226, 475, 687, 703
Eam1104I CTCTTC 1 cut(s) 914
EarI CTCTTC 1 cut(s) 914
Ecl136II GAGCTC 2 cut(s) 250, 274
Eco130I CCWWGG 2 cut(s) 32, 193
Eco24I GRGCYC 2 cut(s) 252, 276
Eco53kI GAGCTC 2 cut(s) 250, 274
Eco57I CTGAAG 1 cut(s) 744
Eco81I CCTNAGG 1 cut(s) 935
Eco88I CYCGRG 2 cut(s) 253, 458
EcoICRI GAGCTC 2 cut(s) 250, 274
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 32, 193
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 2 cut(s) 252, 276
ErhI CCWWGG 2 cut(s) 32, 193
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 7 cut(s) 197, 423, 502, 533, 745, 908, 963
FaqI GGGAC 1 cut(s) 759
FatI CATG 7 cut(s) 193, 419, 498, 529, 741, 904, 959
FauI CCCGC 1 cut(s) 59
FauNDI CATATG 1 cut(s) 814
Fnu4HI GCNGC 2 cut(s) 55, 773
FokI GGATG 5 cut(s) 455, 599, 722, 744, 819
FriOI GRGCYC 2 cut(s) 252, 276
Fsp4HI GCNGC 2 cut(s) 55, 773
FspAI RTGCGCAY 1 cut(s) 811
FspBI CTAG 6 cut(s) 33, 104, 362, 911, 968, 1024
FspI TGCGCA 1 cut(s) 811
GlaI GCGC 2 cut(s) 648, 811
GluI GCNGC 2 cut(s) 55, 773
HapII CCGG 2 cut(s) 473, 707
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 649, 812
Hin1II CATG 7 cut(s) 197, 423, 502, 533, 745, 908, 963
Hin6I GCGC 2 cut(s) 647, 810
HinP1I GCGC 2 cut(s) 647, 810
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HindIII AAGCTT 2 cut(s) 178, 896
HinfI GANTC 9 cut(s) 150, 159, 329, 481, 493, 578, 609, 782, 994
HpaII CCGG 2 cut(s) 473, 707
HphI GGTGA 2 cut(s) 547, 614
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 8 cut(s) 13, 92, 268, 480, 486, 787, 874, 999
Hpy188III TCNNGA 8 cut(s) 104, 286, 440, 460, 707, 722, 761, 824
Hpy8I GTNNAC 1 cut(s) 613
Hpy99I CGWCG 2 cut(s) 92, 245
HpyAV CCTTC 5 cut(s) 206, 445, 532, 686, 722
HpyCH4III ACNGT 2 cut(s) 310, 617
HpyCH4V TGCA 2 cut(s) 562, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 352, 845
HpyF3I CTNAG 5 cut(s) 341, 863, 935, 946, 1016
Hsp92II CATG 7 cut(s) 197, 423, 502, 533, 745, 908, 963
HspAI GCGC 2 cut(s) 647, 810
Kpn2I TCCGGA 1 cut(s) 706
Kzo9I GATC 6 cut(s) 13, 106, 226, 475, 687, 703
LmnI GCTCC 7 cut(s) 66, 125, 271, 279, 895, 939, 1012
LweI GCATC 3 cut(s) 571, 700, 797
MaeI CTAG 6 cut(s) 33, 104, 362, 911, 968, 1024
MaeIII GTNAC 3 cut(s) 302, 399, 490
MalI GATC 6 cut(s) 15, 108, 228, 477, 689, 705
MbiI CCGCTC 1 cut(s) 128
MboI GATC 6 cut(s) 13, 106, 226, 475, 687, 703
MboII GAAGA 3 cut(s) 230, 737, 931
MflI RGATCY 3 cut(s) 13, 106, 226
MhlI GDGCHC 3 cut(s) 252, 276, 473
MluCI AATT 4 cut(s) 7, 111, 752, 827
MlyI GAGTC 5 cut(s) 159, 168, 323, 487, 618
MmeI TCCRAC 3 cut(s) 70, 852, 990
MnlI CCTC 8 cut(s) 262, 274, 336, 360, 389, 419, 645, 941
Mph1103I ATGCAT 1 cut(s) 820
MroI TCCGGA 1 cut(s) 706
MseI TTAA 3 cut(s) 636, 834, 900
MslI CAYNNNNRTG 3 cut(s) 300, 528, 630
MspI CCGG 2 cut(s) 473, 707
MspR9I CCNGG 2 cut(s) 473, 804
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 1 cut(s) 804
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 63, 352, 845
NciI CCSGG 1 cut(s) 473
NcoI CCATGG 1 cut(s) 193
NdeI CATATG 1 cut(s) 814
NdeII GATC 6 cut(s) 13, 106, 226, 475, 687, 703
NlaIII CATG 7 cut(s) 197, 423, 502, 533, 745, 908, 963
NlaIV GGNNCC 2 cut(s) 132, 891
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 3 cut(s) 302, 399, 490
NsbI TGCGCA 1 cut(s) 811
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 3 cut(s) 423, 533, 963
OliI CACNNNNGTG 1 cut(s) 300
PaeR7I CTCGAG 1 cut(s) 458
PciI ACATGT 2 cut(s) 529, 959
PctI GAATGC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 481, 578, 782, 994
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 194
PkrI GCNGC 2 cut(s) 56, 774
PleI GAGTC 5 cut(s) 158, 167, 323, 487, 617
PpsI GAGTC 5 cut(s) 158, 167, 323, 487, 617
PscI ACATGT 2 cut(s) 529, 959
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 2 cut(s) 252, 276
Psp6I CCWGG 1 cut(s) 802
PspGI CCWGG 1 cut(s) 802
PspN4I GGNNCC 2 cut(s) 132, 891
PsrI GAACNNNNNNTAC 2 cut(s) 362, 394
PsuI RGATCY 3 cut(s) 13, 106, 226
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 2 cut(s) 430, 800
RsaNI GTAC 2 cut(s) 429, 799
RseI CAYNNNNRTG 3 cut(s) 300, 528, 630
SacI GAGCTC 2 cut(s) 252, 276
SaqAI TTAA 3 cut(s) 636, 834, 900
SatI GCNGC 2 cut(s) 55, 773
Sau3AI GATC 6 cut(s) 13, 106, 226, 475, 687, 703
SchI GAGTC 5 cut(s) 159, 168, 323, 487, 618
ScrFI CCNGG 2 cut(s) 473, 804
SduI GDGCHC 3 cut(s) 252, 276, 473
SfaNI GCATC 3 cut(s) 571, 700, 797
Sfr274I CTCGAG 1 cut(s) 458
SlaI CTCGAG 1 cut(s) 458
SmiMI CAYNNNNRTG 3 cut(s) 300, 528, 630
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 4 cut(s) 7, 111, 752, 827
SsiI CCGC 5 cut(s) 52, 55, 64, 128, 772
SspMI CTAG 6 cut(s) 33, 104, 362, 911, 968, 1024
SstI GAGCTC 2 cut(s) 252, 276
StyD4I CCNGG 2 cut(s) 471, 802
StyI CCWWGG 2 cut(s) 32, 193
TaaI ACNGT 2 cut(s) 310, 617
TaqI TCGA 2 cut(s) 459, 690
TasI AATT 4 cut(s) 7, 111, 752, 827
TauI GCSGC 2 cut(s) 57, 775
TfiI GAWTC 4 cut(s) 481, 578, 782, 994
Tru1I TTAA 3 cut(s) 636, 834, 900
Tru9I TTAA 3 cut(s) 636, 834, 900
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 3 cut(s) 302, 399, 490
Tsp45I GTSAC 3 cut(s) 302, 399, 490
TspDTI ATGAA 5 cut(s) 515, 727, 746, 867, 932
TspRI CASTG 1 cut(s) 160
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 726
XapI RAATTY 1 cut(s) 827
XbaI TCTAGA 1 cut(s) 103
XceI RCATGY 3 cut(s) 423, 533, 963
XhoI CTCGAG 1 cut(s) 458
XmaJI CCTAGG 1 cut(s) 32
XspI CTAG 6 cut(s) 33, 104, 362, 911, 968, 1024
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.