Prupe.2G159200_v2.0.a1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0004867
Physical Location & Seq
Reverse (-)
21175750 .. 21180954
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G159200.9

Sequence Viewer

Length: 429 bp
ATGGTAGATAGGCACTCAGGATGCCCAATTCCTGATGGCCGGGAGTTGAAGAGTGAAATCCAAAAGGACGGATCTAAAACCTTGTATTATGTGAATTTGGGAACAGGAATACGAGTAAATTCAGTGGAAGATATGTTTTGTTACAAGAATAGCAAATCTGGGGAAAATAATCCCAAGCAGAATTATGTGAATTTGGGAACAGGAATACGAGTAAACTCAGTGGAAGATATGTTTCGTTACAAGGATAGAGAATCTGGGGAAAATACTCCCAAGCAGTTTTCAGACTGGAGGCCGCACCAAAGTCGGCCATCAATTCTGCAGTCAGTGAAGGAAGTTGGCCACAATATCTTCCGTTGCCCCTTAGCGCAACAAGCGGCAATAGTAAAACCTACTGCTGCTGGGCAACTCGCAATACTGCACGCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

143

Amino Acids

15.91

Weight (kDa)

9.03

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 293, 374
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 3 cut(s) 37, 305, 337
AcsI RAATTY 3 cut(s) 94, 118, 190
AdeI CACNNNGTG 1 cut(s) 424
AgsI TTSAA 1 cut(s) 49
AlwI GGATC 1 cut(s) 79
AoxI GGCC 4 cut(s) 37, 290, 305, 337
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 3 cut(s) 94, 118, 190
AspLEI GCGC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 41
BalI TGGCCA 1 cut(s) 339
BbvI GCAGC 1 cut(s) 382
BccI CCATC 2 cut(s) 29, 316
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BcnI CCSGG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 317
BisI GCNGC 3 cut(s) 293, 375, 396
BlsI GCNGC 3 cut(s) 294, 376, 397
Bme1390I CCNGG 1 cut(s) 41
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 361
BpuMI CCSGG 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 290
BseGI GGATG 1 cut(s) 26
BseMII CTCAG 2 cut(s) 30, 231
BseNI ACTGG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 382
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 4 cut(s) 39, 292, 307, 339
BsiSI CCGG 1 cut(s) 40
BsnI GGCC 4 cut(s) 39, 292, 307, 339
Bsp143I GATC 1 cut(s) 71
BspACI CCGC 2 cut(s) 293, 374
BspANI GGCC 4 cut(s) 39, 292, 307, 339
BspCNI CTCAG 2 cut(s) 29, 230
BspMAI CTGCAG 1 cut(s) 321
BspPI GGATC 1 cut(s) 79
BsrI ACTGG 1 cut(s) 290
BssMI GATC 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 3 cut(s) 16, 217, 361
BstF5I GGATG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 1 cut(s) 74
BstMBI GATC 1 cut(s) 71
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 39
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 4 cut(s) 39, 292, 307, 339
BtsCI GGATG 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 129, 225, 330, 429
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 367
CviJI RGCY 4 cut(s) 39, 292, 307, 339
CviKI_1 RGCY 4 cut(s) 39, 292, 307, 339
DdeI CTNAG 3 cut(s) 16, 217, 361
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
DraIII CACNNNGTG 1 cut(s) 424
EaeI YGGCCR 3 cut(s) 37, 305, 337
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
FaiI YATR 4 cut(s) 90, 134, 186, 230
Fnu4HI GCNGC 3 cut(s) 293, 375, 396
FokI GGATG 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 293, 375, 396
GlaI GCGC 1 cut(s) 366
GluI GCNGC 3 cut(s) 293, 375, 396
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 4 cut(s) 39, 292, 307, 339
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 367
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HinfI GANTC 1 cut(s) 251
HpaII CCGG 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 2 cut(s) 18, 32
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 322
HpyCH4V TGCA 2 cut(s) 319, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 3 cut(s) 16, 217, 361
HspAI GCGC 1 cut(s) 365
Kzo9I GATC 1 cut(s) 71
LpnPI CCDG 9 cut(s) 3, 45, 53, 90, 144, 186, 240, 271, 384
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 11
MaeIII GTNAC 2 cut(s) 140, 236
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 4 cut(s) 61, 140, 236, 340
MflI RGATCY 1 cut(s) 71
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 6 cut(s) 27, 94, 118, 181, 190, 312
MluNI TGGCCA 1 cut(s) 339
MnlI CCTC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 339
MscI TGGCCA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 371
NciI CCSGG 1 cut(s) 41
NdeII GATC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 3 cut(s) 294, 376, 397
PspFI CCCAGC 1 cut(s) 398
PstI CTGCAG 1 cut(s) 321
PsuI RGATCY 1 cut(s) 71
SatI GCNGC 3 cut(s) 293, 375, 396
Sau3AI GATC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 41
SetI ASST 2 cut(s) 83, 391
SfaNI GCATC 1 cut(s) 11
SfcI CTRYAG 1 cut(s) 317
Sse9I AATT 6 cut(s) 27, 94, 118, 181, 190, 312
SsiI CCGC 2 cut(s) 293, 374
StyD4I CCNGG 1 cut(s) 39
TasI AATT 6 cut(s) 27, 94, 118, 181, 190, 312
TauI GCSGC 2 cut(s) 295, 377
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 4 cut(s) 129, 225, 330, 429
TseI GCWGC 1 cut(s) 395
TspGWI ACGGA 2 cut(s) 84, 341
TspRI CASTG 4 cut(s) 129, 225, 330, 429
XapI RAATTY 3 cut(s) 94, 118, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.