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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G13560 |
| fragaria_vesca | FvH4_7g20871 FvH4_7g20871 |
| malus_domestica | MD07G1186500.v1.1 MD13G1157000.v1.1 |
| prunus_persica | Prupe.2G225300_v2.0.a1 |
| pyrus_communis | pycom01g14560 pycom07g17780 |
| rosa_chinensis | RchiOBHm_Chr1g0365121 RchiOBHm_Chr7g0221351 |
| rosa_laevigata | RLG00000014849 RLG00000027440 RLG00000028869 |
| rosa_multiflora | Rmu_co8467635.1_g000001 Rmu_sc0001688.1_g000008 |
| rosa_roxburghii | Rroxscaffold_4G00291410 Rroxscaffold_4G00291920 |
| rosa_rugosa | Rorug01G0320000 Rorug01G0320100 |
| rosa_samantha | Rh1AG328700 Rh1BG290300 Rh1CG307000 Rh1DG321900 Rh2DG217200 Rh3AG000700 Rh3BG000500 Rh3CG000500 Rh3DG000700 Rh7AG341600 Rh7CG359500 |
| rosa_wichuraiana | Rw1G029170 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1298 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 92, 320 |
| Acc36I | ACCTGC | 1 cut(s) | 1360 |
| AccB1I | GGYRCC | 1 cut(s) | 125 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 193 |
| AccI | GTMKAC | 1 cut(s) | 577 |
| AccII | CGCG | 1 cut(s) | 426 |
| AciI | CCGC | 3 cut(s) | 424, 1168, 1810 |
| AclWI | GGATC | 1 cut(s) | 907 |
| AcoI | YGGCCR | 1 cut(s) | 106 |
| AcsI | RAATTY | 4 cut(s) | 1478, 1498, 1668, 2000 |
| AcuI | CTGAAG | 6 cut(s) | 141, 302, 776, 789, 896, 1941 |
| AcyI | GRCGYC | 2 cut(s) | 126, 348 |
| AdeI | CACNNNGTG | 1 cut(s) | 659 |
| AfaI | GTAC | 2 cut(s) | 386, 621 |
| AfeI | AGCGCT | 1 cut(s) | 1343 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 193, 514, 1278, 1886 |
| AjnI | CCWGG | 2 cut(s) | 1181, 1673 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1935, 1967 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 537, 569 |
| Alw21I | GWGCWC | 1 cut(s) | 1855 |
| Alw26I | GTCTC | 3 cut(s) | 271, 718, 1744 |
| AlwI | GGATC | 1 cut(s) | 907 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 451 |
| Aor51HI | AGCGCT | 1 cut(s) | 1343 |
| AoxI | GGCC | 3 cut(s) | 106, 1454, 1654 |
| ApeKI | GCWGC | 3 cut(s) | 203, 1154, 1157 |
| ApoI | RAATTY | 4 cut(s) | 1478, 1498, 1668, 2000 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1985, 2017 |
| AseI | ATTAAT | 1 cut(s) | 681 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 432 |
| AspA2I | CCTAGG | 2 cut(s) | 1688, 1880 |
| AspLEI | GCGC | 2 cut(s) | 128, 1344 |
| AspS9I | GGNCC | 7 cut(s) | 66, 142, 485, 1454, 1591, 1655, 1820 |
| AsuC2I | CCSGG | 1 cut(s) | 294 |
| AsuHPI | GGTGA | 4 cut(s) | 265, 419, 2003, 2045 |
| AsuII | TTCGAA | 1 cut(s) | 361 |
| AsuNHI | GCTAGC | 1 cut(s) | 962 |
| AvaII | GGWCC | 5 cut(s) | 66, 142, 485, 1591, 1820 |
| AvrII | CCTAGG | 2 cut(s) | 1688, 1880 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1317, 1350 |
| BanI | GGYRCC | 1 cut(s) | 125 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 603, 635, 855, 887 |
| BauI | CACGAG | 1 cut(s) | 1659 |
| Bbv12I | GWGCWC | 1 cut(s) | 1855 |
| BbvI | GCAGC | 3 cut(s) | 215, 1141, 1169 |
| BccI | CCATC | 3 cut(s) | 508, 1513, 1831 |
| BceAI | ACGGC | 2 cut(s) | 17, 98 |
| BciT130I | CCWGG | 2 cut(s) | 1183, 1675 |
| BciVI | GTATCC | 1 cut(s) | 194 |
| BclI | TGATCA | 1 cut(s) | 1423 |
| BcnI | CCSGG | 1 cut(s) | 294 |
| BcoDI | GTCTC | 3 cut(s) | 271, 718, 1744 |
| BfaI | CTAG | 6 cut(s) | 717, 963, 1689, 1800, 1881, 1931 |
| BfmI | CTRYAG | 3 cut(s) | 214, 1155, 1530 |
| BfoI | RGCGCY | 2 cut(s) | 129, 1345 |
| BfuAI | ACCTGC | 1 cut(s) | 1360 |
| BfuI | GTATCC | 1 cut(s) | 194 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 38 |
| BglII | AGATCT | 2 cut(s) | 583, 2027 |
| BisI | GCNGC | 4 cut(s) | 204, 1155, 1158, 1811 |
| BlnI | CCTAGG | 2 cut(s) | 1688, 1880 |
| BlsI | GCNGC | 4 cut(s) | 205, 1156, 1159, 1812 |
| BmcAI | AGTACT | 1 cut(s) | 621 |
| Bme1390I | CCNGG | 3 cut(s) | 294, 1183, 1675 |
| Bme18I | GGWCC | 5 cut(s) | 66, 142, 485, 1591, 1820 |
| BmgT120I | GGNCC | 7 cut(s) | 66, 142, 485, 1454, 1591, 1655, 1820 |
| BmiI | GGNNCC | 2 cut(s) | 127, 1455 |
| BmrFI | CCNGG | 3 cut(s) | 294, 1183, 1675 |
| BmrI | ACTGGG | 1 cut(s) | 2005 |
| BmsI | GCATC | 3 cut(s) | 794, 1532, 1885 |
| BmtI | GCTAGC | 1 cut(s) | 966 |
| BmuI | ACTGGG | 1 cut(s) | 2005 |
| BpmI | CTGGAG | 4 cut(s) | 473, 561, 1322, 1787 |
| Bpu14I | TTCGAA | 1 cut(s) | 361 |
| BpuEI | CTTGAG | 3 cut(s) | 254, 1139, 1595 |
| BpuMI | CCSGG | 1 cut(s) | 294 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1875 |
| BsaHI | GRCGYC | 2 cut(s) | 126, 348 |
| BsaI | GGTCTC | 1 cut(s) | 1744 |
| BsaJI | CCNNGG | 5 cut(s) | 33, 1149, 1182, 1688, 1880 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 733, 763 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 193, 514, 1278, 1886 |
| Bse118I | RCCGGY | 1 cut(s) | 104 |
| Bse1I | ACTGG | 4 cut(s) | 544, 627, 1770, 2000 |
| Bse3DI | GCAATG | 1 cut(s) | 1266 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1875 |
| BseBI | CCWGG | 2 cut(s) | 1183, 1675 |
| BseDI | CCNNGG | 5 cut(s) | 33, 1149, 1182, 1688, 1880 |
| BseGI | GGATG | 2 cut(s) | 1524, 1876 |
| BseJI | GATNNNNATC | 1 cut(s) | 1875 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 193, 514, 1278, 1886 |
| BseMI | GCAATG | 1 cut(s) | 1266 |
| BseMII | CTCAG | 5 cut(s) | 233, 277, 333, 900, 1074 |
| BseNI | ACTGG | 4 cut(s) | 544, 627, 1770, 2000 |
| BseRI | GAGGAG | 4 cut(s) | 741, 958, 1031, 1592 |
| BseXI | GCAGC | 3 cut(s) | 215, 1141, 1169 |
| BseYI | CCCAGC | 1 cut(s) | 41 |
| BsgI | GTGCAG | 1 cut(s) | 1911 |
| Bsh1236I | CGCG | 1 cut(s) | 426 |
| BshFI | GGCC | 3 cut(s) | 108, 1456, 1656 |
| BshNI | GGYRCC | 1 cut(s) | 125 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1855 |
| BsiSI | CCGG | 2 cut(s) | 105, 293 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 193, 514, 1278, 1886 |
| BsmAI | GTCTC | 3 cut(s) | 271, 718, 1744 |
| BsnI | GGCC | 3 cut(s) | 108, 1456, 1656 |
| Bso31I | GGTCTC | 1 cut(s) | 1744 |
| Bsp119I | TTCGAA | 1 cut(s) | 361 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1855 |
| Bsp143I | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| BspACI | CCGC | 3 cut(s) | 424, 1168, 1810 |
| BspANI | GGCC | 3 cut(s) | 108, 1456, 1656 |
| BspCNI | CTCAG | 5 cut(s) | 234, 276, 334, 901, 1075 |
| BspFNI | CGCG | 1 cut(s) | 426 |
| BspHI | TCATGA | 1 cut(s) | 775 |
| BspLI | GGNNCC | 2 cut(s) | 127, 1455 |
| BspMAI | CTGCAG | 3 cut(s) | 218, 1159, 1534 |
| BspMI | ACCTGC | 1 cut(s) | 1360 |
| BspOI | GCTAGC | 1 cut(s) | 966 |
| BspPI | GGATC | 1 cut(s) | 907 |
| BspT104I | TTCGAA | 1 cut(s) | 361 |
| BspT107I | GGYRCC | 1 cut(s) | 125 |
| BspTNI | GGTCTC | 1 cut(s) | 1744 |
| BsrDI | GCAATG | 1 cut(s) | 1266 |
| BsrFI | RCCGGY | 1 cut(s) | 104 |
| BsrI | ACTGG | 4 cut(s) | 544, 627, 1770, 2000 |
| BssAI | RCCGGY | 1 cut(s) | 104 |
| BssECI | CCNNGG | 5 cut(s) | 33, 1149, 1182, 1688, 1880 |
| BssMI | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| BssNAI | GTATAC | 1 cut(s) | 578 |
| BssNI | GRCGYC | 2 cut(s) | 126, 348 |
| BssSI | CACGAG | 1 cut(s) | 1659 |
| BssT1I | CCWWGG | 3 cut(s) | 1149, 1688, 1880 |
| Bst1107I | GTATAC | 1 cut(s) | 578 |
| Bst2BI | CACGAG | 1 cut(s) | 1659 |
| Bst2UI | CCWGG | 2 cut(s) | 1183, 1675 |
| Bst4CI | ACNGT | 3 cut(s) | 70, 146, 468 |
| Bst6I | CTCTTC | 4 cut(s) | 173, 492, 1182, 1977 |
| BstACI | GRCGYC | 2 cut(s) | 126, 348 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 659, 1163, 1529 |
| BstBI | TTCGAA | 1 cut(s) | 361 |
| BstC8I | GCNNGC | 4 cut(s) | 106, 964, 1048, 1168 |
| BstDEI | CTNAG | 7 cut(s) | 242, 263, 342, 722, 909, 1083, 2024 |
| BstDSI | CCRYGG | 1 cut(s) | 33 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1276, 1884 |
| BstF5I | GGATG | 2 cut(s) | 1524, 1876 |
| BstFNI | CGCG | 1 cut(s) | 426 |
| BstH2I | RGCGCY | 2 cut(s) | 129, 1345 |
| BstHHI | GCGC | 2 cut(s) | 128, 1344 |
| BstKTI | GATC | 6 cut(s) | 17, 586, 915, 1000, 1426, 2030 |
| BstMAI | GTCTC | 3 cut(s) | 271, 718, 1744 |
| BstMBI | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| BstNI | CCWGG | 2 cut(s) | 1183, 1675 |
| BstSCI | CCNGG | 3 cut(s) | 292, 1181, 1673 |
| BstSFI | CTRYAG | 3 cut(s) | 214, 1155, 1530 |
| BstUI | CGCG | 1 cut(s) | 426 |
| BstV1I | GCAGC | 3 cut(s) | 215, 1141, 1169 |
| BstX2I | RGATCY | 3 cut(s) | 583, 912, 2027 |
| BstYI | RGATCY | 3 cut(s) | 583, 912, 2027 |
| BstZ17I | GTATAC | 1 cut(s) | 578 |
| BsuI | GTATCC | 1 cut(s) | 194 |
| BsuRI | GGCC | 3 cut(s) | 108, 1456, 1656 |
| BtgI | CCRYGG | 1 cut(s) | 33 |
| BtsCI | GGATG | 2 cut(s) | 1524, 1876 |
| BtsI | GCAGTG | 2 cut(s) | 1231, 1899 |
| BtsIMutI | CAGTG | 4 cut(s) | 464, 1231, 1899, 1917 |
| BveI | ACCTGC | 1 cut(s) | 1360 |
| Cac8I | GCNNGC | 4 cut(s) | 106, 964, 1048, 1168 |
| CaiI | CAGNNNCTG | 1 cut(s) | 451 |
| CciI | TCATGA | 1 cut(s) | 775 |
| CfoI | GCGC | 2 cut(s) | 128, 1344 |
| Cfr10I | RCCGGY | 1 cut(s) | 104 |
| Cfr13I | GGNCC | 7 cut(s) | 66, 142, 485, 1454, 1591, 1655, 1820 |
| CpoI | CGGWCCG | 1 cut(s) | 66 |
| CseI | GACGC | 2 cut(s) | 356, 746 |
| Csp6I | GTAC | 2 cut(s) | 385, 620 |
| CspI | CGGWCCG | 1 cut(s) | 66 |
| CviAII | CATG | 5 cut(s) | 415, 776, 917, 1275, 1394 |
| CviQI | GTAC | 2 cut(s) | 385, 620 |
| DdeI | CTNAG | 7 cut(s) | 242, 263, 342, 722, 909, 1083, 2024 |
| DinI | GGCGCC | 1 cut(s) | 127 |
| DpnI | GATC | 6 cut(s) | 16, 585, 914, 999, 1425, 2029 |
| DpnII | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| DraI | TTTAAA | 1 cut(s) | 166 |
| DraIII | CACNNNGTG | 1 cut(s) | 659 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 92, 320 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 92, 320 |
| EaeI | YGGCCR | 1 cut(s) | 106 |
| Eam1104I | CTCTTC | 4 cut(s) | 173, 492, 1182, 1977 |
| EarI | CTCTTC | 4 cut(s) | 173, 492, 1182, 1977 |
| Eco130I | CCWWGG | 3 cut(s) | 1149, 1688, 1880 |
| Eco31I | GGTCTC | 1 cut(s) | 1744 |
| Eco47I | GGWCC | 5 cut(s) | 66, 142, 485, 1591, 1820 |
| Eco47III | AGCGCT | 1 cut(s) | 1343 |
| Eco57I | CTGAAG | 6 cut(s) | 141, 302, 776, 789, 896, 1941 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1276, 1884 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1454 |
| EcoRI | GAATTC | 1 cut(s) | 2000 |
| EcoRII | CCWGG | 2 cut(s) | 1181, 1673 |
| EcoT14I | CCWWGG | 3 cut(s) | 1149, 1688, 1880 |
| EgeI | GGCGCC | 1 cut(s) | 127 |
| EheI | GGCGCC | 1 cut(s) | 127 |
| ErhI | CCWWGG | 3 cut(s) | 1149, 1688, 1880 |
| FaeI | CATG | 5 cut(s) | 418, 779, 920, 1278, 1397 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 420, 452, 816, 848 |
| FatI | CATG | 5 cut(s) | 414, 775, 916, 1274, 1393 |
| FauI | CCCGC | 1 cut(s) | 1175 |
| FbaI | TGATCA | 1 cut(s) | 1423 |
| FblI | GTMKAC | 1 cut(s) | 577 |
| Fnu4HI | GCNGC | 4 cut(s) | 204, 1155, 1158, 1811 |
| FokI | GGATG | 2 cut(s) | 1531, 1863 |
| Fsp4HI | GCNGC | 4 cut(s) | 204, 1155, 1158, 1811 |
| FspBI | CTAG | 6 cut(s) | 717, 963, 1689, 1800, 1881, 1931 |
| GlaI | GCGC | 2 cut(s) | 127, 1343 |
| GluI | GCNGC | 4 cut(s) | 204, 1155, 1158, 1811 |
| GsaI | CCCAGC | 1 cut(s) | 45 |
| GsuI | CTGGAG | 4 cut(s) | 473, 561, 1322, 1787 |
| HaeII | RGCGCY | 2 cut(s) | 129, 1345 |
| HaeIII | GGCC | 3 cut(s) | 108, 1456, 1656 |
| HapII | CCGG | 2 cut(s) | 105, 293 |
| HgaI | GACGC | 2 cut(s) | 356, 746 |
| HhaI | GCGC | 2 cut(s) | 128, 1344 |
| Hin1I | GRCGYC | 2 cut(s) | 126, 348 |
| Hin1II | CATG | 5 cut(s) | 418, 779, 920, 1278, 1397 |
| Hin6I | GCGC | 2 cut(s) | 126, 1342 |
| HinP1I | GCGC | 2 cut(s) | 126, 1342 |
| HincII | GTYRAC | 1 cut(s) | 1375 |
| HindII | GTYRAC | 1 cut(s) | 1375 |
| HindIII | AAGCTT | 3 cut(s) | 284, 594, 731 |
| HinfI | GANTC | 9 cut(s) | 86, 392, 475, 902, 953, 1544, 1771, 1943, 2036 |
| HpaII | CCGG | 2 cut(s) | 105, 293 |
| HphI | GGTGA | 4 cut(s) | 265, 419, 2003, 2045 |
| Hpy166II | GTNNAC | 3 cut(s) | 410, 578, 1375 |
| Hpy8I | GTNNAC | 3 cut(s) | 410, 578, 1375 |
| Hpy99I | CGWCG | 2 cut(s) | 95, 2009 |
| HpyCH4III | ACNGT | 3 cut(s) | 70, 146, 468 |
| HpyCH4V | TGCA | 9 cut(s) | 216, 563, 653, 662, 836, 974, 1157, 1532, 1892 |
| HpyF3I | CTNAG | 7 cut(s) | 242, 263, 342, 722, 909, 1083, 2024 |
| Hsp92I | GRCGYC | 2 cut(s) | 126, 348 |
| Hsp92II | CATG | 5 cut(s) | 418, 779, 920, 1278, 1397 |
| HspAI | GCGC | 2 cut(s) | 126, 1342 |
| KasI | GGCGCC | 1 cut(s) | 125 |
| KroI | GCCGGC | 1 cut(s) | 104 |
| KroNI | GCCGGC | 1 cut(s) | 106 |
| Ksp22I | TGATCA | 1 cut(s) | 1423 |
| Kzo9I | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| LmnI | GCTCC | 7 cut(s) | 221, 648, 971, 1642, 1699, 1729, 1850 |
| Lsp1109I | GCAGC | 3 cut(s) | 215, 1141, 1169 |
| LweI | GCATC | 3 cut(s) | 794, 1532, 1885 |
| MaeI | CTAG | 6 cut(s) | 717, 963, 1689, 1800, 1881, 1931 |
| MaeIII | GTNAC | 1 cut(s) | 1991 |
| MalI | GATC | 6 cut(s) | 16, 585, 914, 999, 1425, 2029 |
| MboI | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| MflI | RGATCY | 3 cut(s) | 583, 912, 2027 |
| MhlI | GDGCHC | 1 cut(s) | 1855 |
| Mly113I | GGCGCC | 1 cut(s) | 126 |
| MlyI | GAGTC | 2 cut(s) | 80, 386 |
| MmeI | TCCRAC | 3 cut(s) | 121, 382, 1799 |
| MroNI | GCCGGC | 1 cut(s) | 104 |
| MroXI | GAANNNNTTC | 1 cut(s) | 432 |
| MseI | TTAA | 9 cut(s) | 165, 174, 674, 681, 821, 1140, 1311, 1695, 1724 |
| MspA1I | CMGCKG | 2 cut(s) | 206, 1082 |
| MspI | CCGG | 2 cut(s) | 105, 293 |
| MspR9I | CCNGG | 3 cut(s) | 294, 1183, 1675 |
| MvaI | CCWGG | 2 cut(s) | 1183, 1675 |
| MvnI | CGCG | 1 cut(s) | 426 |
| NaeI | GCCGGC | 1 cut(s) | 106 |
| NarI | GGCGCC | 1 cut(s) | 126 |
| NciI | CCSGG | 1 cut(s) | 294 |
| NdeII | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| NgoMIV | GCCGGC | 1 cut(s) | 104 |
| NheI | GCTAGC | 1 cut(s) | 962 |
| NlaIII | CATG | 5 cut(s) | 418, 779, 920, 1278, 1397 |
| NlaIV | GGNNCC | 2 cut(s) | 127, 1455 |
| NmeAIII | GCCGAG | 1 cut(s) | 1955 |
| NmuCI | GTSAC | 1 cut(s) | 1991 |
| NspV | TTCGAA | 1 cut(s) | 361 |
| PagI | TCATGA | 1 cut(s) | 775 |
| PdiI | GCCGGC | 1 cut(s) | 106 |
| PdmI | GAANNNNTTC | 1 cut(s) | 432 |
| PfeI | GAWTC | 7 cut(s) | 475, 902, 953, 1544, 1771, 1943, 2036 |
| PflFI | GACNNNGTC | 1 cut(s) | 89 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 193 |
| PfoI | TCCNGGA | 1 cut(s) | 1673 |
| PkrI | GCNGC | 4 cut(s) | 205, 1156, 1159, 1812 |
| PleI | GAGTC | 2 cut(s) | 80, 386 |
| PluTI | GGCGCC | 1 cut(s) | 129 |
| PpsI | GAGTC | 2 cut(s) | 80, 386 |
| PshBI | ATTAAT | 1 cut(s) | 681 |
| PsiI | TTATAA | 1 cut(s) | 1298 |
| Psp6I | CCWGG | 2 cut(s) | 1181, 1673 |
| PspFI | CCCAGC | 1 cut(s) | 41 |
| PspGI | CCWGG | 2 cut(s) | 1181, 1673 |
| PspN4I | GGNNCC | 2 cut(s) | 127, 1455 |
| PspPI | GGNCC | 7 cut(s) | 66, 142, 485, 1454, 1591, 1655, 1820 |
| PstI | CTGCAG | 3 cut(s) | 218, 1159, 1534 |
| PstNI | CAGNNNCTG | 1 cut(s) | 451 |
| PsuI | RGATCY | 3 cut(s) | 583, 912, 2027 |
| PsyI | GACNNNGTC | 1 cut(s) | 89 |
| PvuII | CAGCTG | 2 cut(s) | 206, 1082 |
| RsaI | GTAC | 2 cut(s) | 386, 621 |
| RsaNI | GTAC | 2 cut(s) | 385, 620 |
| Rsr2I | CGGWCCG | 1 cut(s) | 66 |
| RsrII | CGGWCCG | 1 cut(s) | 66 |
| SaqAI | TTAA | 9 cut(s) | 165, 174, 674, 681, 821, 1140, 1311, 1695, 1724 |
| SatI | GCNGC | 4 cut(s) | 204, 1155, 1158, 1811 |
| Sau3AI | GATC | 6 cut(s) | 14, 583, 912, 997, 1423, 2027 |
| Sau96I | GGNCC | 7 cut(s) | 66, 142, 485, 1454, 1591, 1655, 1820 |
| SbfI | CCTGCAGG | 1 cut(s) | 218 |
| ScaI | AGTACT | 1 cut(s) | 621 |
| SchI | GAGTC | 2 cut(s) | 80, 386 |
| ScrFI | CCNGG | 3 cut(s) | 294, 1183, 1675 |
| SdaI | CCTGCAGG | 1 cut(s) | 218 |
| SduI | GDGCHC | 1 cut(s) | 1855 |
| SfaNI | GCATC | 3 cut(s) | 794, 1532, 1885 |
| SfcI | CTRYAG | 3 cut(s) | 214, 1155, 1530 |
| SfoI | GGCGCC | 1 cut(s) | 127 |
| SfuI | TTCGAA | 1 cut(s) | 361 |
| SinI | GGWCC | 5 cut(s) | 66, 142, 485, 1591, 1820 |
| SmlI | CTYRAG | 3 cut(s) | 233, 1118, 1574 |
| SmoI | CTYRAG | 3 cut(s) | 233, 1118, 1574 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 218 |
| SsiI | CCGC | 3 cut(s) | 424, 1168, 1810 |
| SspDI | GGCGCC | 1 cut(s) | 125 |
| SspI | AATATT | 1 cut(s) | 1586 |
| SspMI | CTAG | 6 cut(s) | 717, 963, 1689, 1800, 1881, 1931 |
| StyD4I | CCNGG | 3 cut(s) | 292, 1181, 1673 |
| StyI | CCWWGG | 3 cut(s) | 1149, 1688, 1880 |
| TaaI | ACNGT | 3 cut(s) | 70, 146, 468 |
| TaqI | TCGA | 6 cut(s) | 273, 324, 361, 844, 1259, 2004 |
| TatI | WGTACW | 2 cut(s) | 384, 619 |
| TauI | GCSGC | 1 cut(s) | 1813 |
| TfiI | GAWTC | 7 cut(s) | 475, 902, 953, 1544, 1771, 1943, 2036 |
| Tru1I | TTAA | 9 cut(s) | 165, 174, 674, 681, 821, 1140, 1311, 1695, 1724 |
| Tru9I | TTAA | 9 cut(s) | 165, 174, 674, 681, 821, 1140, 1311, 1695, 1724 |
| TscAI | CASTG | 4 cut(s) | 471, 1238, 1899, 1924 |
| TseFI | GTSAC | 1 cut(s) | 1991 |
| TseI | GCWGC | 3 cut(s) | 203, 1154, 1157 |
| Tsp45I | GTSAC | 1 cut(s) | 1991 |
| TspDTI | ATGAA | 9 cut(s) | 510, 545, 624, 630, 720, 1373, 1395, 1785, 2028 |
| TspGWI | ACGGA | 1 cut(s) | 79 |
| TspRI | CASTG | 4 cut(s) | 471, 1238, 1899, 1924 |
| Tth111I | GACNNNGTC | 1 cut(s) | 89 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 193 |
| VpaK11BI | GGWCC | 5 cut(s) | 66, 142, 485, 1591, 1820 |
| VspI | ATTAAT | 1 cut(s) | 681 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1276, 1884 |
| XapI | RAATTY | 4 cut(s) | 1478, 1498, 1668, 2000 |
| XmaJI | CCTAGG | 2 cut(s) | 1688, 1880 |
| XmiI | GTMKAC | 1 cut(s) | 577 |
| XmnI | GAANNNNTTC | 1 cut(s) | 432 |
| XspI | CTAG | 6 cut(s) | 717, 963, 1689, 1800, 1881, 1931 |
| ZrmI | AGTACT | 1 cut(s) | 621 |