Prupe.8G256400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003414
Physical Location & Seq
Forward (+)
21872884 .. 21876442
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G256400.2

Sequence Viewer

Length: 1338 bp
ATGTATAAGCAAACAAAGCATATGCGTTGTTTTGAGACAATTCCAGATTCTGATCAGAGCATTTCCGATGAGGAAGAATCTGACCAAGATTGGCCCAACAAACGCCTTACCCTTGACGGATCCGCCAAGAAAAAGGAGGAATTACCGCTTTCATCACGGCTAGAAATGCTCAAAGCTGGTGCGCATGTGGATGCCTGCAACTTGAAGGATGACGTGAGCTCTGGCGATGAGTTGCAGAAAGATGATGTTCATAGCATTGGAAAAGAAGACGGATCATACACATGGTCTGCGGCAAGTAAAGAAGTTGAGGAACTTATGCGTTCGAATGAGAAAAAGTCATGTTCATCGTCACATTCTTCTTACTTGAAATTGAATAAATCATCTAAAGGAGTCAAAGGCAAAGGCAAGCCAAAGTTTTCGTTCCGTTTCCCAACACATAAGGAAGGACACTCTTGGCTTTCTGTATATAAGGATGACAATGATGTTTCATTCAAGGTTCAAGAATTGCCTGAGAGGTTGGATTATAGAACTGAAGAAAATTCAGATGCTGAGCTTCTTGAGGATATTCAGGTTGAGGAGGAAAATCAGTTGGAGATTGTGCCTTTTGAAGTCAATGAACATGGGCATGGCTGTATTGAGCAGTCAATGGCAGAGCTTTTAGATGGCCTTCAGGATAAGACGACTATGGCAAGAGGAAGTTCCAAAATGTATAGTAGAAAAAGATGTAAAAGGGGGCAGCCTGTTGTGAAGAGAGTACCCCCACTTGGAGATAGACTTATCGACAGTGAAAGCTTCCTTGCGCACTTAAGCCCTGAATCGCCAAGTGATAGCGAGGCTGATGATCAAATTTTGAAGCTTGATAAACCGGAGGTGAAGAGGCAAACATTGGTAGATAGATTTCAGGAAGCTTTAAGTGACAGAGCCCTTGTTACAGTGCCTAAAACATTAAGAATTGGATTATTTGGGCAACTGCAGCAGGTCGTTCAGAGTGAAAAAGAAAGTGATATGGAATTCTTAAAGAATATTGAGAACGGAGCTAACCAAAAGGAACCAAGCTGCATCGATGTGAAAATTTGTTCGAGACACCTGGATGCAAAGCTGACAGTTTGCCACTGCTCCTTTGGCAATAAGTTAAAGGTCATGAGCCTCCCACGGTCAGAGAGCCCCAAGCACATGCTGAACGAAGAAACAACACAGATAGTTATTTTCAATCCAAGAGTTTGCAATGATGTTGACCTTGATGCTGGGAAATGGATTCGTATTCACGCTCCATGGAAGGAGATTCATGTGGGCAATGACAAAAGCATTATCCTATCCACTCATTTCTCCGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

446

Amino Acids

50.49

Weight (kDa)

5.56

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 183, 801
Acc36I ACCTGC 1 cut(s) 967
AciI CCGC 3 cut(s) 123, 146, 290
AclWI GGATC 3 cut(s) 114, 127, 280
AcsI RAATTY 4 cut(s) 538, 846, 1010, 1071
AcuI CTGAAG 2 cut(s) 552, 653
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 1 cut(s) 764
AflII CTTAAG 1 cut(s) 805
AgsI TTSAA 8 cut(s) 205, 367, 373, 493, 500, 608, 853, 1210
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 1086
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 2 cut(s) 29, 1075
AlwI GGATC 3 cut(s) 114, 127, 280
AlwNI CAGNNNCTG 2 cut(s) 50, 548
AoxI GGCC 2 cut(s) 92, 664
ApeKI GCWGC 3 cut(s) 736, 973, 1056
ApoI RAATTY 4 cut(s) 538, 846, 1010, 1071
AspLEI GCGC 2 cut(s) 184, 802
AspS9I GGNCC 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 883
AsuII TTCGAA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 119
BanII GRGCYC 3 cut(s) 221, 925, 1166
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 3 cut(s) 748, 985, 1043
BccI CCATC 1 cut(s) 656
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 1088
BclI TGATCA 2 cut(s) 52, 841
BcoDI GTCTC 2 cut(s) 29, 1075
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 971
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 1 cut(s) 967
BisI GCNGC 4 cut(s) 291, 737, 974, 1057
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 4 cut(s) 292, 738, 975, 1058
Bme1390I CCNGG 1 cut(s) 1088
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 2 cut(s) 121, 1050
BmrFI CCNGG 1 cut(s) 1088
BmsI GCATC 5 cut(s) 181, 535, 1068, 1081, 1231
BpiI GAAGAC 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 1062
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 1151, 1271
BsaWI WCCGGW 1 cut(s) 865
Bsc4I CCNNNNNNNGG 1 cut(s) 764
Bse3DI GCAATG 2 cut(s) 1231, 1300
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 1 cut(s) 1088
BseCI ATCGAT 1 cut(s) 1062
BseDI CCNNGG 2 cut(s) 1151, 1271
BseGI GGATG 4 cut(s) 196, 214, 478, 1096
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 764
BseMI GCAATG 2 cut(s) 1231, 1300
BseMII CTCAG 2 cut(s) 501, 540
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 3 cut(s) 748, 985, 1043
BseYI CCCAGC 1 cut(s) 1244
BshFI GGCC 2 cut(s) 94, 666
BshVI ATCGAT 1 cut(s) 1062
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 1 cut(s) 866
BslI CCNNNNNNNGG 1 cut(s) 764
BsmAI GTCTC 2 cut(s) 29, 1075
BsnI GGCC 2 cut(s) 94, 666
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 221, 925, 1166
Bsp143I GATC 4 cut(s) 52, 119, 272, 841
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 1271
BspACI CCGC 3 cut(s) 123, 146, 290
BspANI GGCC 2 cut(s) 94, 666
BspCNI CTCAG 2 cut(s) 502, 541
BspDI ATCGAT 1 cut(s) 1062
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 121, 1050
BspMAI CTGCAG 1 cut(s) 975
BspMI ACCTGC 1 cut(s) 967
BspPI GGATC 3 cut(s) 114, 127, 280
BspT104I TTCGAA 1 cut(s) 323
BspTI CTTAAG 1 cut(s) 805
BsrDI GCAATG 2 cut(s) 1231, 1300
BssECI CCNNGG 2 cut(s) 1151, 1271
BssMI GATC 4 cut(s) 52, 119, 272, 841
BssT1I CCWWGG 1 cut(s) 1271
Bst2UI CCWGG 1 cut(s) 1088
Bst4CI ACNGT 4 cut(s) 785, 934, 1105, 1155
Bst6I CTCTTC 2 cut(s) 743, 869
BstAFI CTTAAG 1 cut(s) 805
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 196, 407
BstDEI CTNAG 2 cut(s) 510, 549
BstDSI CCRYGG 2 cut(s) 1151, 1271
BstF5I GGATG 4 cut(s) 196, 214, 478, 1096
BstHHI GCGC 2 cut(s) 184, 802
BstKTI GATC 4 cut(s) 55, 122, 275, 844
BstMAI GTCTC 2 cut(s) 29, 1075
BstMBI GATC 4 cut(s) 52, 119, 272, 841
BstMWI GCNNNNNNNGC 4 cut(s) 16, 166, 973, 1122
BstNI CCWGG 1 cut(s) 1088
BstNSI RCATGY 2 cut(s) 188, 1177
BstSCI CCNGG 1 cut(s) 1086
BstSFI CTRYAG 1 cut(s) 971
BstV1I GCAGC 3 cut(s) 748, 985, 1043
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
Bsu15I ATCGAT 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 94, 666
BsuTUI ATCGAT 1 cut(s) 1062
BtgI CCRYGG 2 cut(s) 1151, 1271
BtgZI GCGATG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 4 cut(s) 196, 214, 478, 1096
BtsI GCAGTG 1 cut(s) 1111
BtsIMutI CAGTG 3 cut(s) 790, 939, 1111
BveI ACCTGC 1 cut(s) 967
Cac8I GCNNGC 2 cut(s) 196, 407
CaiI CAGNNNCTG 2 cut(s) 50, 548
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 2 cut(s) 184, 802
Cfr13I GGNCC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 1062
Csp6I GTAC 1 cut(s) 755
CviAII CATG 9 cut(s) 185, 282, 339, 620, 626, 1141, 1174, 1272, 1286
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 2 cut(s) 510, 549
DpnI GATC 4 cut(s) 54, 121, 274, 843
DpnII GATC 4 cut(s) 52, 119, 272, 841
Eam1104I CTCTTC 2 cut(s) 743, 869
EarI CTCTTC 2 cut(s) 743, 869
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 1271
Eco24I GRGCYC 3 cut(s) 221, 925, 1166
Eco53kI GAGCTC 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 552, 653
EcoICRI GAGCTC 1 cut(s) 219
EcoRI GAATTC 1 cut(s) 1010
EcoRII CCWGG 1 cut(s) 1086
EcoT14I CCWWGG 1 cut(s) 1271
EcoT38I GRGCYC 3 cut(s) 221, 925, 1166
ErhI CCWWGG 1 cut(s) 1271
FaeI CATG 9 cut(s) 188, 285, 342, 623, 629, 1144, 1177, 1275, 1289
FatI CATG 9 cut(s) 184, 281, 338, 619, 625, 1140, 1173, 1271, 1285
FauNDI CATATG 1 cut(s) 21
FbaI TGATCA 2 cut(s) 52, 841
Fnu4HI GCNGC 4 cut(s) 291, 737, 974, 1057
FokI GGATG 4 cut(s) 203, 221, 485, 1103
FriOI GRGCYC 3 cut(s) 221, 925, 1166
Fsp4HI GCNGC 4 cut(s) 291, 737, 974, 1057
FspAI RTGCGCAY 1 cut(s) 183
FspBI CTAG 1 cut(s) 161
FspI TGCGCA 2 cut(s) 183, 801
GlaI GCGC 2 cut(s) 183, 801
GluI GCNGC 4 cut(s) 291, 737, 974, 1057
GsaI CCCAGC 1 cut(s) 1248
HaeIII GGCC 2 cut(s) 94, 666
HapII CCGG 1 cut(s) 866
HhaI GCGC 2 cut(s) 184, 802
Hin1II CATG 9 cut(s) 188, 285, 342, 623, 629, 1144, 1177, 1275, 1289
Hin6I GCGC 2 cut(s) 182, 800
HinP1I GCGC 2 cut(s) 182, 800
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HindIII AAGCTT 3 cut(s) 790, 854, 906
HinfI GANTC 6 cut(s) 47, 77, 390, 815, 1255, 1282
HpaII CCGG 1 cut(s) 866
HphI GGTGA 1 cut(s) 883
Hpy166II GTNNAC 1 cut(s) 1234
Hpy188I TCNGA 8 cut(s) 52, 57, 67, 82, 544, 987, 1159, 1330
Hpy188III TCNNGA 7 cut(s) 44, 500, 557, 671, 902, 1080, 1141
Hpy8I GTNNAC 1 cut(s) 1234
HpyAV CCTTC 4 cut(s) 199, 437, 677, 1270
HpyCH4III ACNGT 4 cut(s) 785, 934, 1105, 1155
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 6 cut(s) 198, 235, 973, 1059, 1094, 1224
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 166, 973, 1122
HpyF3I CTNAG 2 cut(s) 510, 549
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 9 cut(s) 188, 285, 342, 623, 629, 1144, 1177, 1275, 1289
HspAI GCGC 2 cut(s) 182, 800
Ksp22I TGATCA 2 cut(s) 52, 841
Kzo9I GATC 4 cut(s) 52, 119, 272, 841
LmnI GCTCC 3 cut(s) 1034, 1121, 1273
Lsp1109I GCAGC 3 cut(s) 748, 985, 1043
LweI GCATC 5 cut(s) 181, 535, 1068, 1081, 1231
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 348, 914, 928
MalI GATC 4 cut(s) 54, 121, 274, 843
MboI GATC 4 cut(s) 52, 119, 272, 841
MboII GAAGA 7 cut(s) 86, 278, 348, 545, 760, 886, 1196
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 3 cut(s) 221, 925, 1166
MluCI AATT 9 cut(s) 39, 140, 368, 503, 538, 846, 951, 1010, 1071
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 2 cut(s) 498, 570
MseI TTAA 5 cut(s) 806, 911, 947, 1016, 1133
MslI CAYNNNNRTG 5 cut(s) 189, 280, 624, 1064, 1089
MspCI CTTAAG 1 cut(s) 805
MspI CCGG 1 cut(s) 866
MspR9I CCNGG 1 cut(s) 1088
MvaI CCWGG 1 cut(s) 1088
MwoI GCNNNNNNNGC 4 cut(s) 16, 166, 973, 1122
NcoI CCATGG 1 cut(s) 1271
NdeI CATATG 1 cut(s) 21
NdeII GATC 4 cut(s) 52, 119, 272, 841
NlaIII CATG 9 cut(s) 188, 285, 342, 623, 629, 1144, 1177, 1275, 1289
NlaIV GGNNCC 2 cut(s) 121, 1050
NmuCI GTSAC 2 cut(s) 348, 914
NsbI TGCGCA 2 cut(s) 183, 801
NspI RCATGY 2 cut(s) 188, 1177
NspV TTCGAA 1 cut(s) 323
PagI TCATGA 1 cut(s) 1140
PfeI GAWTC 5 cut(s) 47, 77, 815, 1255, 1282
PkrI GCNGC 4 cut(s) 292, 738, 975, 1058
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 1086
PspFI CCCAGC 1 cut(s) 1244
PspGI CCWGG 1 cut(s) 1086
PspN4I GGNNCC 2 cut(s) 121, 1050
PspPI GGNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 975
PstNI CAGNNNCTG 2 cut(s) 50, 548
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
RseI CAYNNNNRTG 5 cut(s) 189, 280, 624, 1064, 1089
SacI GAGCTC 1 cut(s) 221
SaqAI TTAA 5 cut(s) 806, 911, 947, 1016, 1133
SatI GCNGC 4 cut(s) 291, 737, 974, 1057
Sau3AI GATC 4 cut(s) 52, 119, 272, 841
Sau96I GGNCC 1 cut(s) 93
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 1 cut(s) 1088
SduI GDGCHC 3 cut(s) 221, 925, 1166
SfaNI GCATC 5 cut(s) 181, 535, 1068, 1081, 1231
SfcI CTRYAG 1 cut(s) 971
SfuI TTCGAA 1 cut(s) 323
SmiMI CAYNNNNRTG 5 cut(s) 189, 280, 624, 1064, 1089
SmlI CTYRAG 2 cut(s) 557, 805
SmoI CTYRAG 2 cut(s) 557, 805
Sse9I AATT 9 cut(s) 39, 140, 368, 503, 538, 846, 951, 1010, 1071
SsiI CCGC 3 cut(s) 123, 146, 290
SspI AATATT 1 cut(s) 1024
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 1086
StyI CCWWGG 1 cut(s) 1271
TaaI ACNGT 4 cut(s) 785, 934, 1105, 1155
TaiI ACGT 1 cut(s) 216
TaqI TCGA 4 cut(s) 323, 780, 1062, 1079
TasI AATT 9 cut(s) 39, 140, 368, 503, 538, 846, 951, 1010, 1071
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 5 cut(s) 47, 77, 815, 1255, 1282
Tru1I TTAA 5 cut(s) 806, 911, 947, 1016, 1133
Tru9I TTAA 5 cut(s) 806, 911, 947, 1016, 1133
TscAI CASTG 3 cut(s) 790, 939, 1118
TseFI GTSAC 2 cut(s) 348, 914
TseI GCWGC 3 cut(s) 736, 973, 1056
Tsp45I GTSAC 2 cut(s) 348, 914
TspDTI ATGAA 6 cut(s) 141, 239, 333, 477, 630, 1274
TspGWI ACGGA 4 cut(s) 132, 285, 413, 1047
TspRI CASTG 3 cut(s) 790, 939, 1118
Vha464I CTTAAG 1 cut(s) 805
XapI RAATTY 4 cut(s) 538, 846, 1010, 1071
XceI RCATGY 2 cut(s) 188, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.