Rorug06G0234100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003414
Physical Location & Seq
Reverse (-)
37647211 .. 37651292
4082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0234100.1

Sequence Viewer

Length: 1560 bp
ATGATGAATAAGCGATTGAAAACGAGCAGCGACAAGGCTGTCGTCAATGTTTGGCAGCGACAGCTGGGCGAGATCTCCGCCAGAAACTTTGCTCACCGTCTCGGTGGCTCTGAGGATCTCGTGCTTCGACTTGATGTCCACAGGAAGCTGGACAAGCATAGAGGATGTGTGAACACCGTAAGCTTCAATGCAGATGGCGATATTTTGCTTTCGGGTTCTGATGACAGGCGGCTCATACTATGGAACTGGGAAACGGGGAAAGTTAAGTTGTCATTCCATTCCGGTCATAATAGCAATGTTTTCCAAGCAAAGTTCATGCCTTATAGTGATGATCGAAGCATTGTCACTTGTGCTGCTGATGGGCAGGTGAGACATACTCAGATTTTAGAACGAGGACAAGTGGAAAGTTCAAAGTTGGCCAGACACCGTGGACGCGCTCATAAGTTAGCTATTGAGCCTGGGAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGGTGCAACATTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCAAACACTTGATGGTAGAAGGAGTTACTTGCCAGTTGTCAGTCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCAGTCGCAGGTTCTGATGAGTATACCCGGCTTTTTGATATCCGCAAGTATAACTGGGATGGATCATCTGATATTGGTCAACCCACGAACTACTTCTGCCCACCACATTTGATTGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATACAATGACGAGTTCATCTATCTTTTTACACGAGAAATGGGATTAGGACCTAATCCTCCCATTTCCATGAGTAGTGATGCAAGTGAAATGAGTGATGATCACGAGTCTGAAGAATCTCCATCATCCATGGATACTGATGAAAAAACTGTTCCTCAAGTTTACAAGGGTCACTTGAACTCTGAGACAGTGAAAGGTGTGAACTTCTTTGGGCCGAAATGCGAGTATGTTGTGAGTGGGTCTGACTGTGGGCGGATATTCATATGGAAGAAAAAGGGAGGTGAGTTGATTCGTGTCATGGAAGGAGATAAGGATGTAGTAAACTGTATCAAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAAAATGATATTAAGATGTGGACTCCAAAGGCTCTAGAGAGAGCTACTCTGCCTCAGAACATTGAACAGGTCTCGATACCTGGCCGATTAATATGGTACGCTATTGGTGATGAGGATGAGGATGATGAATATTCTTATGACGACTATATGTTTGATGACGATGACAATGACGAGAACGATGAAGATGATGAGGACGAGGATGACGATGACAATGATAGTGATGATCAGGACGAGGATGACAATGATAGTGATGATCAGGATGAGGATGATGGTGGTGGTGATGGTGACAATGTTGAGAATGAAAATGGCAGAAACTGTGAAGATGATGATGATGCTAACAATCATGATAGTTTTCTTGATGGTTATGATCTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

519

Amino Acids

58.29

Weight (kDa)

4.42

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 5 - 134 1.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 43 - 143 1e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 45 - 183 1.9e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 46 - 171 1.3e-12 WDR3 first beta-propeller domain
WD40 PF00400 48 - 82 1e-06 WD domain, G-beta repeat
WDR55 PF24796 49 - 174 7e-08 WDR55
Beta-prop_WDR3_2nd PF25172 49 - 171 4.1e-06 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 50 - 226 2.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 53 - 124 6.2e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 57 - 230 1.1e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 110 - 185 4.8e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 315 - 401 9.9e-14 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
AasI GACNNNNNNGTC 1 cut(s) 38
Acc36I ACCTGC 2 cut(s) 355, 635
AccI GTMKAC 1 cut(s) 659
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 78, 229, 679, 1063
AclWI GGATC 2 cut(s) 123, 706
AcoI YGGCCR 2 cut(s) 417, 1264
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 2 cut(s) 1164, 1280
AgsI TTSAA 6 cut(s) 19, 187, 411, 988, 1184, 1247
AhdI GACNNNNNGTC 1 cut(s) 134
AjnI CCWGG 2 cut(s) 457, 1261
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 6 cut(s) 64, 148, 183, 449, 802, 1226
AluI AGCT 6 cut(s) 64, 148, 183, 449, 802, 1226
Alw26I GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
AlwI GGATC 2 cut(s) 123, 706
AlwNI CAGNNNCTG 2 cut(s) 650, 1497
AoxI GGCC 4 cut(s) 417, 780, 1022, 1264
ApeKI GCWGC 3 cut(s) 27, 55, 353
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
AseI ATTAAT 1 cut(s) 1271
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 860, 1022
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 7 cut(s) 86, 379, 500, 1103, 1301, 1472, 1478
AvaII GGWCC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 2 cut(s) 467, 532
BauI CACGAG 3 cut(s) 119, 843, 914
BbvI GCAGC 3 cut(s) 39, 67, 340
BccI CCATC 9 cut(s) 188, 353, 488, 557, 689, 940, 1445, 1457, 1535
BciT130I CCWGG 2 cut(s) 459, 1263
BciVI GTATCC 1 cut(s) 937
BclI TGATCA 3 cut(s) 910, 1405, 1435
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BfaI CTAG 1 cut(s) 1217
BfuAI ACCTGC 2 cut(s) 355, 635
BfuI GTATCC 1 cut(s) 937
BglII AGATCT 1 cut(s) 72
BisI GCNGC 4 cut(s) 28, 56, 230, 354
BlsI GCNGC 4 cut(s) 29, 57, 231, 355
Bme1390I CCNGG 3 cut(s) 459, 664, 1263
Bme18I GGWCC 1 cut(s) 860
BmeRI GACNNNNNGTC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 860, 1022
BmiI GGNNCC 2 cut(s) 464, 531
BmrFI CCNGG 3 cut(s) 459, 664, 1263
BmrI ACTGGG 3 cut(s) 256, 534, 700
BmsI GCATC 2 cut(s) 880, 1504
BmuI ACTGGG 3 cut(s) 256, 534, 700
BplI GAGNNNNNCTC 4 cut(s) 361, 393, 1213, 1245
BpuEI CTTGAG 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 664
BsaI GGTCTC 1 cut(s) 1258
BsaJI CCNNGG 4 cut(s) 427, 458, 939, 1158
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bse1I ACTGG 5 cut(s) 251, 504, 529, 584, 695
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 459, 1263
BseDI CCNNGG 4 cut(s) 427, 458, 939, 1158
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 5 cut(s) 102, 392, 532, 984, 1250
BseNI ACTGG 5 cut(s) 251, 504, 529, 584, 695
BseXI GCAGC 3 cut(s) 39, 67, 340
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 4 cut(s) 419, 782, 1024, 1266
BsiSI CCGG 2 cut(s) 282, 664
BsmAI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 4 cut(s) 419, 782, 1024, 1266
Bso31I GGTCTC 1 cut(s) 1258
Bsp1286I GDGCHC 2 cut(s) 467, 532
Bsp143I GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
Bsp19I CCATGG 2 cut(s) 939, 1158
BspACI CCGC 4 cut(s) 78, 229, 679, 1063
BspANI GGCC 4 cut(s) 419, 782, 1024, 1266
BspCNI CTCAG 5 cut(s) 103, 391, 531, 985, 1249
BspFNI CGCG 1 cut(s) 435
BspHI TCATGA 1 cut(s) 1525
BspLI GGNNCC 2 cut(s) 464, 531
BspMI ACCTGC 2 cut(s) 355, 635
BspPI GGATC 2 cut(s) 123, 706
BspTNI GGTCTC 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 5 cut(s) 251, 504, 529, 584, 695
BssECI CCNNGG 4 cut(s) 427, 458, 939, 1158
BssMI GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
BssNAI GTATAC 1 cut(s) 660
BssSI CACGAG 3 cut(s) 119, 843, 914
BssT1I CCWWGG 2 cut(s) 939, 1158
Bst1107I GTATAC 1 cut(s) 660
Bst2BI CACGAG 3 cut(s) 119, 843, 914
Bst2UI CCWGG 2 cut(s) 459, 1263
Bst4CI ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1499
BstC8I GCNNGC 1 cut(s) 1171
BstDEI CTNAG 5 cut(s) 111, 378, 518, 993, 1236
BstDSI CCRYGG 3 cut(s) 427, 939, 1158
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 9 cut(s) 75, 118, 334, 517, 701, 913, 1408, 1438, 1552
BstMAI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BstMBI GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
BstMWI GCNNNNNNNGC 2 cut(s) 61, 154
BstNI CCWGG 2 cut(s) 459, 1263
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 3 cut(s) 457, 662, 1261
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 39, 67, 340
BstX2I RGATCY 2 cut(s) 72, 115
BstYI RGATCY 2 cut(s) 72, 115
BstZ17I GTATAC 1 cut(s) 660
BsuI GTATCC 1 cut(s) 937
BsuRI GGCC 4 cut(s) 419, 782, 1024, 1266
BtgI CCRYGG 3 cut(s) 427, 939, 1158
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 2 cut(s) 1005, 1180
BveI ACCTGC 2 cut(s) 355, 635
Cac8I GCNNGC 1 cut(s) 1171
CaiI CAGNNNCTG 2 cut(s) 650, 1497
CciI TCATGA 1 cut(s) 1525
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 860, 1022
CseI GACGC 1 cut(s) 441
Csp6I GTAC 2 cut(s) 1163, 1279
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 7 cut(s) 316, 549, 880, 940, 1108, 1159, 1526
CviQI GTAC 2 cut(s) 1163, 1279
DdeI CTNAG 5 cut(s) 111, 378, 518, 993, 1236
DpnI GATC 9 cut(s) 74, 117, 333, 516, 700, 912, 1407, 1437, 1551
DpnII GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
DrdI GACNNNNNNGTC 1 cut(s) 38
DriI GACNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 417, 1264
Eam1105I GACNNNNNGTC 1 cut(s) 134
EciI GGCGGA 2 cut(s) 67, 1078
Eco130I CCWWGG 2 cut(s) 939, 1158
Eco24I GRGCYC 2 cut(s) 467, 532
Eco31I GGTCTC 1 cut(s) 1258
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 860
Eco57I CTGAAG 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 457, 1261
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 2 cut(s) 939, 1158
EcoT38I GRGCYC 2 cut(s) 467, 532
ErhI CCWWGG 2 cut(s) 939, 1158
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1529
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FatI CATG 7 cut(s) 315, 548, 879, 939, 1107, 1158, 1525
FauNDI CATATG 1 cut(s) 1073
FbaI TGATCA 3 cut(s) 910, 1405, 1435
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 4 cut(s) 28, 56, 230, 354
FriOI GRGCYC 2 cut(s) 467, 532
Fsp4HI GCNGC 4 cut(s) 28, 56, 230, 354
FspBI CTAG 1 cut(s) 1217
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 28, 56, 230, 354
GsaI CCCAGC 1 cut(s) 68
HaeIII GGCC 4 cut(s) 419, 782, 1024, 1266
HapII CCGG 2 cut(s) 282, 664
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 437
Hin1II CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1529
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 716
HindII GTYRAC 1 cut(s) 716
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 4 cut(s) 917, 926, 1099, 1204
HpaII CCGG 2 cut(s) 282, 664
HphI GGTGA 7 cut(s) 86, 379, 500, 1103, 1301, 1472, 1478
Hpy166II GTNNAC 9 cut(s) 139, 172, 431, 660, 716, 973, 1012, 1132, 1203
Hpy188III TCNNGA 7 cut(s) 914, 1217, 1255, 1409, 1439, 1526, 1538
Hpy8I GTNNAC 9 cut(s) 139, 172, 431, 660, 716, 973, 1012, 1132, 1203
HpyAV CCTTC 2 cut(s) 564, 1106
HpyCH4III ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1499
HpyCH4V TGCA 7 cut(s) 191, 505, 602, 608, 638, 893, 1169
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 154
HpyF3I CTNAG 5 cut(s) 111, 378, 518, 993, 1236
Hsp92II CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1529
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 3 cut(s) 910, 1405, 1435
Kzo9I GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
LmnI GCTCC 2 cut(s) 462, 535
Lsp1109I GCAGC 3 cut(s) 39, 67, 340
LweI GCATC 2 cut(s) 880, 1504
MaeI CTAG 1 cut(s) 1217
MaeIII GTNAC 4 cut(s) 343, 575, 980, 1466
MalI GATC 9 cut(s) 74, 117, 333, 516, 700, 912, 1407, 1437, 1551
MboI GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
MboII GAAGA 5 cut(s) 503, 935, 1090, 1376, 1514
MfeI CAATTG 2 cut(s) 603, 609
MflI RGATCY 2 cut(s) 72, 115
MhlI GDGCHC 2 cut(s) 467, 532
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 4 cut(s) 603, 609, 768, 1179
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 2 cut(s) 926, 1198
MmeI TCCRAC 1 cut(s) 745
Mox20I TGGCCA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 419
MseI TTAA 3 cut(s) 264, 1194, 1271
MslI CAYNNNNRTG 1 cut(s) 878
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 2 cut(s) 282, 664
MspR9I CCNGG 3 cut(s) 459, 664, 1263
MunI CAATTG 2 cut(s) 603, 609
MvaI CCWGG 2 cut(s) 459, 1263
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 61, 154
NciI CCSGG 1 cut(s) 664
NcoI CCATGG 2 cut(s) 939, 1158
NdeI CATATG 1 cut(s) 1073
NdeII GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
NlaIII CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1529
NlaIV GGNNCC 2 cut(s) 464, 531
NmuCI GTSAC 3 cut(s) 343, 980, 1466
NspI RCATGY 1 cut(s) 552
PagI TCATGA 1 cut(s) 1525
PaqCI CACCTGC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 1383
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 2 cut(s) 926, 1099
PkrI GCNGC 4 cut(s) 29, 57, 231, 355
PleI GAGTC 2 cut(s) 925, 1198
PpsI GAGTC 2 cut(s) 925, 1198
PpuMI RGGWCCY 1 cut(s) 860
PshBI ATTAAT 1 cut(s) 1271
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 2 cut(s) 457, 1261
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 2 cut(s) 457, 1261
PspN4I GGNNCC 2 cut(s) 464, 531
PspPI GGNCC 2 cut(s) 860, 1022
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 2 cut(s) 650, 1497
PsuI RGATCY 2 cut(s) 72, 115
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 1164, 1280
RsaNI GTAC 2 cut(s) 1163, 1279
RseI CAYNNNNRTG 1 cut(s) 878
SaqAI TTAA 3 cut(s) 264, 1194, 1271
SatI GCNGC 4 cut(s) 28, 56, 230, 354
Sau3AI GATC 9 cut(s) 72, 115, 331, 514, 698, 910, 1405, 1435, 1549
Sau96I GGNCC 2 cut(s) 860, 1022
SchI GAGTC 2 cut(s) 926, 1198
ScrFI CCNGG 3 cut(s) 459, 664, 1263
SduI GDGCHC 2 cut(s) 467, 532
SfaNI GCATC 2 cut(s) 880, 1504
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 1 cut(s) 966
SmoI CTYRAG 1 cut(s) 966
Sse9I AATT 4 cut(s) 603, 609, 768, 1179
SsiI CCGC 4 cut(s) 78, 229, 679, 1063
SspI AATATT 1 cut(s) 1313
SspMI CTAG 1 cut(s) 1217
StyD4I CCNGG 3 cut(s) 457, 662, 1261
StyI CCWWGG 2 cut(s) 939, 1158
TaaI ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1499
TaqI TCGA 3 cut(s) 127, 334, 1256
TasI AATT 4 cut(s) 603, 609, 768, 1179
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 926, 1099
Tru1I TTAA 3 cut(s) 264, 1194, 1271
Tru9I TTAA 3 cut(s) 264, 1194, 1271
TscAI CASTG 2 cut(s) 1005, 1180
TseFI GTSAC 3 cut(s) 343, 980, 1466
TseI GCWGC 3 cut(s) 27, 55, 353
Tsp45I GTSAC 3 cut(s) 343, 980, 1466
TspDTI ATGAA 8 cut(s) 20, 304, 817, 966, 1060, 1323, 1377, 1497
TspRI CASTG 2 cut(s) 1005, 1180
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 1 cut(s) 1271
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 1 cut(s) 552
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.