Rroxscaffold_7G00174290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003414
Physical Location & Seq
Reverse (-)
13987667 .. 13991124
3458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00174290.1

Sequence Viewer

Length: 1464 bp
ATGGCTTGGTTTCAGACAATTCCAGATTCTGATCAGAGCATTTCTGATGAGGAAGAACCGGACCAAAATTGGGCGAACAAATGCGCTACGCTCGGCGGAACCACAGAGAAAAAGGAATTGTTACAAATTTCATCTCGGCTCGAAATGCTCAAAGGTGCGAATGCGAAGGCTTGCAATTTCGATGAAGTTAGCTCTGACGATGAGGTGAACAATGTGGTGTCAAAAGTTTCTGTTAATGGCCGTGCTAAAGAGTTGCGAAAAAAAGAGGATGTTCATAGCTTTTGGAGCGATAAACAAGACGAATTACACTCGTGGTCTGCTGCGAGTAAAGAAGCTGAGGCACTCATGCGTTTGAATGAAAAAACTTTTTGCTCATCGCCACTGTCTGTTTACTCGAATTTAAAGAAATCATCTAAAGGAGCGAAAGGCAAAGGGAAACCGAAATTTTTGTTCCGTTTTCCAACACACAAGGAAGGAACCTCGTCCCATTCTATATCTAAGAATGATAATGAAGTGTCATTCAGGGTTCAAGAACTGCCTGAAAGGTTGGATGCAATTGAGCCTAGAAATGTAGATCATTCAGATGTTGAACTTATTGAGGATATTCAGGGGGAAGAGGAATCAGAGATTGTGCTTAGAAATGAAGAACATTCAGATTTTGAACTTCTTGAGGACATTTCGGGGAAAGAGGAAAGTTTGTTAGAGGTTGTGCCTTTTGAAGCCAAGGCTATTCGGCATGGATGTACGGGGCAGTCAATGGCTGAGCTTCTAGATGGCCTTCAAGATAAGACGACTATTTGGAGAAAAAGTTCTAGAAAGTGTTTGCTTCAGCATAGTAGAAAACGACGTAAAAGGAAGCAGCCTGCTGTAAAGTGTGCATCTCCTCTGGGAGATGGAACTATTGACAGTGAAAGCTCTCCTGAGCACTTGGGTCATGGATATCCAAGTGATATCAAGATTGATGATCAAAGTTTGAAGCTTGCTAATCCAGAAATGAAGAGGCAGACATTGGTAGATCGATTTCAGGAAGCTGTGAGTGACAGACTCCTTGTTGCAGTGCCCAAAACTTTAAAGAGTGGATTATTTGGGAAATTGCAGCAGGTGGTGCAGAGTGAGAAAGAAACTGATATAGAATTCTTGAAGAAGATCCAGGAGGGAGCTAGCGAAAGTAATGACCCAAACTGCATGGATGTGAAGATTCTTTCAAAATACCTGGATGCAAAGCTTACCGTGTGTCACTGCTCCTTCGGTAATAACTCAAAGCGCCTCCCATCGCCAGAGGTCTGTGAAGAAATGGTTGACGAAGGACCGGAAAGGACTATTATTTTCAATCCACGAGTTTGCAATGATATTGACCTTGATATTGGGAAATGGATTCGTATACACCCTCCATGGAGGGAGATCCGTGTCGGCACTGCTCAAAACATAATCCTGTCGACATATTTCTCTGAAATTTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

54.95

Weight (kDa)

5.46

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1090
Acc36I ACCTGC 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 1381, 1436
AciI CCGC 1 cut(s) 96
AclWI GGATC 2 cut(s) 1141, 1396
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 5 cut(s) 126, 397, 443, 1133, 1452
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 1 cut(s) 70
AjnI CCWGG 2 cut(s) 1149, 1212
AloI GAACNNNNNNTCC 2 cut(s) 793, 825
AluBI AGCT 9 cut(s) 192, 279, 335, 766, 915, 979, 1031, 1160, 1225
AluI AGCT 9 cut(s) 192, 279, 335, 766, 915, 979, 1031, 1160, 1225
Alw21I GWGCWC 1 cut(s) 927
AlwI GGATC 2 cut(s) 1141, 1396
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 2 cut(s) 238, 775
ApeKI GCWGC 3 cut(s) 320, 859, 1096
ApoI RAATTY 5 cut(s) 126, 397, 443, 1133, 1452
Asp700I GAANNNNTTC 1 cut(s) 808
AspLEI GCGC 2 cut(s) 86, 1266
AspS9I GGNCC 2 cut(s) 61, 1307
AsuHPI GGTGA 1 cut(s) 217
AsuNHI GCTAGC 1 cut(s) 1160
AvaII GGWCC 2 cut(s) 61, 1307
BaeGI GKGCMC 1 cut(s) 1062
BauI CACGAG 2 cut(s) 310, 1335
Bbv12I GWGCWC 1 cut(s) 927
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 3 cut(s) 307, 871, 1108
BccI CCATC 3 cut(s) 767, 887, 1279
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 2 cut(s) 1151, 1214
BclI TGATCA 2 cut(s) 31, 964
BfaI CTAG 4 cut(s) 564, 770, 813, 1161
BfoI RGCGCY 1 cut(s) 1267
BfuAI ACCTGC 1 cut(s) 1090
BisI GCNGC 3 cut(s) 321, 860, 1097
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 3 cut(s) 322, 861, 1098
Bme1390I CCNGG 2 cut(s) 1151, 1214
Bme18I GGWCC 2 cut(s) 61, 1307
BmgT120I GGNCC 2 cut(s) 61, 1307
BmiI GGNNCC 2 cut(s) 100, 478
BmrFI CCNGG 2 cut(s) 1151, 1214
BmsI GCATC 3 cut(s) 541, 887, 1207
BmtI GCTAGC 1 cut(s) 1164
Bpu10I CCTNAGC 2 cut(s) 336, 921
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 689
Bsa29I ATCGAT 1 cut(s) 1018
BsaBI GATNNNNATC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 723, 1391
BsaWI WCCGGW 2 cut(s) 58, 1309
BsaXI ACNNNNNCTCC 2 cut(s) 793, 823
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 1351
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 2 cut(s) 1151, 1214
BseCI ATCGAT 1 cut(s) 1018
BseDI CCNNGG 2 cut(s) 723, 1391
BseGI GGATG 5 cut(s) 274, 556, 746, 1195, 1222
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 1 cut(s) 1351
BseMII CTCAG 3 cut(s) 327, 753, 912
BseRI GAGGAG 1 cut(s) 873
BseSI GKGCMC 1 cut(s) 1062
BseXI GCAGC 3 cut(s) 307, 871, 1108
BsgI GTGCAG 1 cut(s) 1127
BshFI GGCC 2 cut(s) 240, 777
BshVI ATCGAT 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 927
BsiSI CCGG 2 cut(s) 59, 1310
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 469
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 2 cut(s) 240, 777
Bsp1286I GDGCHC 2 cut(s) 927, 1062
Bsp143I GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 1391
BspACI CCGC 1 cut(s) 96
BspANI GGCC 2 cut(s) 240, 777
BspCNI CTCAG 3 cut(s) 328, 754, 913
BspDI ATCGAT 1 cut(s) 1018
BspLI GGNNCC 2 cut(s) 100, 478
BspMI ACCTGC 1 cut(s) 1090
BspOI GCTAGC 1 cut(s) 1164
BspPI GGATC 2 cut(s) 1141, 1396
BsrDI GCAATG 1 cut(s) 1351
BssECI CCNNGG 2 cut(s) 723, 1391
BssMI GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
BssNAI GTATAC 1 cut(s) 1382
BssSI CACGAG 2 cut(s) 310, 1335
BssT1I CCWWGG 2 cut(s) 723, 1391
Bst1107I GTATAC 1 cut(s) 1382
Bst2BI CACGAG 2 cut(s) 310, 1335
Bst2UI CCWGG 2 cut(s) 1151, 1214
Bst4CI ACNGT 3 cut(s) 384, 908, 1231
Bst6I CTCTTC 2 cut(s) 609, 992
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstC8I GCNNGC 4 cut(s) 172, 864, 981, 1162
BstDEI CTNAG 5 cut(s) 336, 498, 635, 762, 921
BstDSI CCRYGG 1 cut(s) 1391
BstF5I GGATG 5 cut(s) 274, 556, 746, 1195, 1222
BstH2I RGCGCY 1 cut(s) 1267
BstHHI GCGC 2 cut(s) 86, 1266
BstKTI GATC 6 cut(s) 34, 577, 967, 1018, 1149, 1404
BstMBI GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
BstMWI GCNNNNNNNGC 3 cut(s) 145, 285, 1105
BstNI CCWGG 2 cut(s) 1151, 1214
BstSCI CCNGG 2 cut(s) 1149, 1212
BstSLI GKGCMC 1 cut(s) 1062
BstV1I GCAGC 3 cut(s) 307, 871, 1108
BstX2I RGATCY 2 cut(s) 1146, 1401
BstYI RGATCY 2 cut(s) 1146, 1401
BstZ17I GTATAC 1 cut(s) 1382
Bsu15I ATCGAT 1 cut(s) 1018
BsuRI GGCC 2 cut(s) 240, 777
BsuTUI ATCGAT 1 cut(s) 1018
BtgI CCRYGG 1 cut(s) 1391
BtgZI GCGATG 2 cut(s) 360, 1257
BtsCI GGATG 5 cut(s) 274, 556, 746, 1195, 1222
BtsI GCAGTG 3 cut(s) 1062, 1237, 1413
BtsIMutI CAGTG 5 cut(s) 380, 913, 1062, 1237, 1413
BveI ACCTGC 1 cut(s) 1090
Cac8I GCNNGC 4 cut(s) 172, 864, 981, 1162
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 2 cut(s) 86, 1266
Cfr13I GGNCC 2 cut(s) 61, 1307
ClaI ATCGAT 1 cut(s) 1018
Csp6I GTAC 1 cut(s) 744
CviAII CATG 5 cut(s) 346, 737, 935, 1186, 1392
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 5 cut(s) 336, 498, 635, 762, 921
DpnI GATC 6 cut(s) 33, 576, 966, 1017, 1148, 1403
DpnII GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
DraI TTTAAA 2 cut(s) 402, 1071
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 609, 992
EarI CTCTTC 2 cut(s) 609, 992
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 723, 1391
Eco32I GATATC 2 cut(s) 941, 952
Eco47I GGWCC 2 cut(s) 61, 1307
Eco57I CTGAAG 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 2 cut(s) 1149, 1212
EcoRV GATATC 2 cut(s) 941, 952
EcoT14I CCWWGG 2 cut(s) 723, 1391
ErhI CCWWGG 2 cut(s) 723, 1391
FaeI CATG 5 cut(s) 349, 740, 938, 1189, 1395
FaqI GGGAC 1 cut(s) 469
FatI CATG 5 cut(s) 345, 736, 934, 1185, 1391
FbaI TGATCA 2 cut(s) 31, 964
FblI GTMKAC 2 cut(s) 1381, 1436
Fnu4HI GCNGC 3 cut(s) 321, 860, 1097
FokI GGATG 5 cut(s) 281, 563, 753, 1202, 1229
Fsp4HI GCNGC 3 cut(s) 321, 860, 1097
FspBI CTAG 4 cut(s) 564, 770, 813, 1161
GlaI GCGC 2 cut(s) 85, 1265
GluI GCNGC 3 cut(s) 321, 860, 1097
HaeII RGCGCY 1 cut(s) 1267
HaeIII GGCC 2 cut(s) 240, 777
HapII CCGG 2 cut(s) 59, 1310
HhaI GCGC 2 cut(s) 86, 1266
Hin1II CATG 5 cut(s) 349, 740, 938, 1189, 1395
Hin6I GCGC 2 cut(s) 84, 1264
HinP1I GCGC 2 cut(s) 84, 1264
HincII GTYRAC 2 cut(s) 1300, 1437
HindII GTYRAC 2 cut(s) 1300, 1437
HindIII AAGCTT 2 cut(s) 977, 1223
HinfI GANTC 5 cut(s) 26, 620, 1044, 1198, 1375
HpaII CCGG 2 cut(s) 59, 1310
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 5 cut(s) 208, 391, 1300, 1382, 1437
Hpy188I TCNGA 9 cut(s) 15, 31, 36, 46, 196, 583, 625, 655, 1450
Hpy8I GTNNAC 5 cut(s) 208, 391, 1300, 1382, 1437
Hpy99I CGWCG 1 cut(s) 849
HpyAV CCTTC 5 cut(s) 160, 467, 788, 1255, 1298
HpyCH4III ACNGT 3 cut(s) 384, 908, 1231
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 9 cut(s) 174, 554, 878, 1055, 1096, 1108, 1185, 1220, 1344
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 285, 1105
HpyF3I CTNAG 5 cut(s) 336, 498, 635, 762, 921
HpySE526I ACGT 1 cut(s) 847
Hsp92II CATG 5 cut(s) 349, 740, 938, 1189, 1395
HspAI GCGC 2 cut(s) 84, 1264
Ksp22I TGATCA 2 cut(s) 31, 964
Kzo9I GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
LmnI GCTCC 4 cut(s) 285, 419, 1157, 1247
Lsp1109I GCAGC 3 cut(s) 307, 871, 1108
LweI GCATC 3 cut(s) 541, 887, 1207
MaeI CTAG 4 cut(s) 564, 770, 813, 1161
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 3 cut(s) 120, 1037, 1235
MalI GATC 6 cut(s) 33, 576, 966, 1017, 1148, 1403
MboI GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
MboII GAAGA 8 cut(s) 65, 626, 656, 1009, 1153, 1156, 1207, 1301
MfeI CAATTG 1 cut(s) 555
MflI RGATCY 2 cut(s) 1146, 1401
MhlI GDGCHC 2 cut(s) 927, 1062
MlyI GAGTC 1 cut(s) 1038
MmeI TCCRAC 2 cut(s) 485, 528
MroXI GAANNNNTTC 1 cut(s) 808
MseI TTAA 3 cut(s) 234, 401, 1070
MslI CAYNNNNRTG 2 cut(s) 582, 1190
MspI CCGG 2 cut(s) 59, 1310
MspR9I CCNGG 2 cut(s) 1151, 1214
MunI CAATTG 1 cut(s) 555
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 2 cut(s) 1151, 1214
MwoI GCNNNNNNNGC 3 cut(s) 145, 285, 1105
NcoI CCATGG 1 cut(s) 1391
NdeII GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
NheI GCTAGC 1 cut(s) 1160
NlaIII CATG 5 cut(s) 349, 740, 938, 1189, 1395
NlaIV GGNNCC 2 cut(s) 100, 478
NmeAIII GCCGAG 2 cut(s) 72, 115
NmuCI GTSAC 2 cut(s) 1037, 1235
PaqCI CACCTGC 1 cut(s) 1090
PctI GAATGC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 4 cut(s) 26, 620, 1198, 1375
PfoI TCCNGGA 1 cut(s) 1149
PkrI GCNGC 3 cut(s) 322, 861, 1098
PleI GAGTC 1 cut(s) 1038
PpsI GAGTC 1 cut(s) 1038
Psp6I CCWGG 2 cut(s) 1149, 1212
PspGI CCWGG 2 cut(s) 1149, 1212
PspN4I GGNNCC 2 cut(s) 100, 478
PspPI GGNCC 2 cut(s) 61, 1307
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 1146, 1401
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
RseI CAYNNNNRTG 2 cut(s) 582, 1190
SalI GTCGAC 1 cut(s) 1435
SaqAI TTAA 3 cut(s) 234, 401, 1070
SatI GCNGC 3 cut(s) 321, 860, 1097
Sau3AI GATC 6 cut(s) 31, 574, 964, 1015, 1146, 1401
Sau96I GGNCC 2 cut(s) 61, 1307
SchI GAGTC 1 cut(s) 1038
ScrFI CCNGG 2 cut(s) 1151, 1214
SduI GDGCHC 2 cut(s) 927, 1062
SfaNI GCATC 3 cut(s) 541, 887, 1207
SinI GGWCC 2 cut(s) 61, 1307
SmiMI CAYNNNNRTG 2 cut(s) 582, 1190
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SsiI CCGC 1 cut(s) 96
SspMI CTAG 4 cut(s) 564, 770, 813, 1161
StyD4I CCNGG 2 cut(s) 1149, 1212
StyI CCWWGG 2 cut(s) 723, 1391
TaaI ACNGT 3 cut(s) 384, 908, 1231
TaiI ACGT 1 cut(s) 850
TaqI TCGA 5 cut(s) 141, 180, 395, 1018, 1436
TfiI GAWTC 4 cut(s) 26, 620, 1198, 1375
Tru1I TTAA 3 cut(s) 234, 401, 1070
Tru9I TTAA 3 cut(s) 234, 401, 1070
TscAI CASTG 5 cut(s) 387, 913, 1062, 1244, 1420
TseFI GTSAC 2 cut(s) 1037, 1235
TseI GCWGC 3 cut(s) 320, 859, 1096
Tsp45I GTSAC 2 cut(s) 1037, 1235
TspDTI ATGAA 7 cut(s) 120, 198, 263, 372, 525, 657, 1010
TspGWI ACGGA 2 cut(s) 443, 1394
TspRI CASTG 5 cut(s) 387, 913, 1062, 1244, 1420
VpaK11BI GGWCC 2 cut(s) 61, 1307
XapI RAATTY 5 cut(s) 126, 397, 443, 1133, 1452
XbaI TCTAGA 2 cut(s) 769, 812
XmiI GTMKAC 2 cut(s) 1381, 1436
XmnI GAANNNNTTC 1 cut(s) 808
XspI CTAG 4 cut(s) 564, 770, 813, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.