Rorug06G0233900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003414
Physical Location & Seq
Reverse (-)
37639540 .. 37641604
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0233900.1

Sequence Viewer

Length: 657 bp
ATGGAAGCCCTAACGACCTCCTCCTCTTCTGCTACCTTCATGTCCTCCTCTTCCCCCTTTTCGATTTTCTCCCCAAGAAGAAAAGCCCTCTCCTTCAGACCCTTTCACCTCCCGCTCGCTTCCAAAGGAAACGAAAATGGCTCCAATCCTCCTCAGTCGGATTCCAAAGAAACCAAGAATCTTCCGGTTCTCAGTAATCGCCACCTCTCCCTCTCCCCACTCTCCAAGGATGCGGCAATGGGATTGGTGATGGGTGCTGCGACAGGAAGAGGGTGGACTACAGGTTCTGGGATGGAGGGGCCTCCTGCACCTGCTGGGATTGAATCGCAATCAAGCACTGAGAATGTCTCCACTTTTCCTTGGTCTCTCTTTACAAAGTCTCCTCGCCGTAGAATGCTCGTGGCTTTCACTTGCAACATTTGTGGGCAGCGGACAACTCGGGCAATCAATCCCCATGCATTCACTGATGGCACTGTCTTTGTGCAGTGTTGTGGATGCAATGCCTTCCATAAGCTCGTGGATAATTTGAACCTGTTTCATGAGATGAAGTGCTATGTGAGCCCAGGCTTTGATTACAAAGGTAATGGATGGGATGCTGTTAACTTCAAATACCTGGAAATGGACGATGGTGATGATGAAATATTTCCCTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.78

Weight (kDa)

7.64

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-DNL PF05180 134 - 184 1e-15 DNL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 319
AccBSI CCGCTC 1 cut(s) 115
AciI CCGC 3 cut(s) 113, 233, 430
AcuI CTGAAG 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 619
AgsI TTSAA 3 cut(s) 323, 529, 607
AjnI CCWGG 2 cut(s) 562, 612
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 1 cut(s) 514
AluI AGCT 1 cut(s) 514
Alw26I GTCTC 3 cut(s) 352, 369, 384
AlwNI CAGNNNCTG 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 2 cut(s) 257, 427
Asp700I GAANNNNTTC 1 cut(s) 642
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 3 cut(s) 98, 259, 641
AvaI CYCGRG 1 cut(s) 438
BanII GRGCYC 1 cut(s) 563
BauI CACGAG 2 cut(s) 398, 515
BbvI GCAGC 2 cut(s) 244, 439
BccI CCATC 5 cut(s) 244, 286, 461, 582, 620
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 2 cut(s) 564, 614
BcoDI GTCTC 3 cut(s) 352, 369, 384
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 319
BisI GCNGC 3 cut(s) 234, 258, 428
BlsI GCNGC 3 cut(s) 235, 259, 429
Bme1390I CCNGG 2 cut(s) 564, 614
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 142, 300
BmrFI CCNGG 2 cut(s) 564, 614
BmsI GCATC 3 cut(s) 220, 485, 583
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 225, 359, 562
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse3DI GCAATG 2 cut(s) 243, 505
BseBI CCWGG 2 cut(s) 564, 614
BseDI CCNNGG 3 cut(s) 225, 359, 562
BseGI GGATG 5 cut(s) 235, 297, 500, 593, 598
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 2 cut(s) 243, 505
BseMII CTCAG 3 cut(s) 167, 205, 330
BseRI GAGGAG 5 cut(s) 10, 13, 37, 141, 372
BseXI GCAGC 2 cut(s) 244, 439
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 2 cut(s) 291, 503
BshFI GGCC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 3 cut(s) 352, 369, 384
BsmI GAATGC 2 cut(s) 399, 458
BsnI GGCC 1 cut(s) 301
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 563
BspACI CCGC 3 cut(s) 113, 233, 430
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 3 cut(s) 166, 204, 331
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 142, 300
BspMI ACCTGC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 369
BsrBI CCGCTC 1 cut(s) 115
BsrDI GCAATG 2 cut(s) 243, 505
BssECI CCNNGG 3 cut(s) 225, 359, 562
BssSI CACGAG 2 cut(s) 398, 515
BssT1I CCWWGG 2 cut(s) 225, 359
Bst2BI CACGAG 2 cut(s) 398, 515
Bst2UI CCWGG 2 cut(s) 564, 614
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 3 cut(s) 31, 55, 262
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 3 cut(s) 153, 191, 339
BstF5I GGATG 5 cut(s) 235, 297, 500, 593, 598
BstMAI GTCTC 3 cut(s) 352, 369, 384
BstMWI GCNNNNNNNGC 1 cut(s) 558
BstNI CCWGG 2 cut(s) 564, 614
BstSCI CCNGG 2 cut(s) 562, 612
BstSFI CTRYAG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 244, 439
BsuRI GGCC 1 cut(s) 301
BtsCI GGATG 5 cut(s) 235, 297, 500, 593, 598
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 4 cut(s) 336, 462, 471, 491
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 117
CaiI CAGNNNCTG 1 cut(s) 287
CciI TCATGA 1 cut(s) 538
Cfr13I GGNCC 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 3 cut(s) 40, 455, 539
CviJI RGCY 8 cut(s) 8, 86, 141, 301, 404, 514, 561, 567
CviKI_1 RGCY 8 cut(s) 8, 86, 141, 301, 404, 514, 561, 567
DdeI CTNAG 3 cut(s) 153, 191, 339
Eam1104I CTCTTC 3 cut(s) 31, 55, 262
EarI CTCTTC 3 cut(s) 31, 55, 262
Eco130I CCWWGG 2 cut(s) 225, 359
Eco24I GRGCYC 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 562, 612
EcoT14I CCWWGG 2 cut(s) 225, 359
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 563
ErhI CCWWGG 2 cut(s) 225, 359
FaeI CATG 3 cut(s) 43, 458, 542
FaiI YATR 5 cut(s) 41, 456, 510, 540, 555
FatI CATG 3 cut(s) 39, 454, 538
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 234, 258, 428
FokI GGATG 5 cut(s) 242, 304, 507, 600, 605
FriOI GRGCYC 1 cut(s) 563
Fsp4HI GCNGC 3 cut(s) 234, 258, 428
GluI GCNGC 3 cut(s) 234, 258, 428
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 1 cut(s) 301
HapII CCGG 1 cut(s) 185
Hin1II CATG 3 cut(s) 43, 458, 542
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 3 cut(s) 161, 178, 323
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 1 cut(s) 185
HphI GGTGA 3 cut(s) 98, 259, 641
Hpy166II GTNNAC 2 cut(s) 276, 601
Hpy188I TCNGA 2 cut(s) 98, 160
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 2 cut(s) 276, 601
HpyAV CCTTC 3 cut(s) 46, 103, 514
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4V TGCA 5 cut(s) 308, 414, 458, 484, 498
HpyF10VI GCNNNNNNNGC 1 cut(s) 558
HpyF3I CTNAG 3 cut(s) 153, 191, 339
Hsp92II CATG 3 cut(s) 43, 458, 542
KspAI GTTAAC 1 cut(s) 601
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 244, 439
LweI GCATC 3 cut(s) 220, 485, 583
MbiI CCGCTC 1 cut(s) 115
MboII GAAGA 5 cut(s) 18, 42, 90, 173, 279
MhlI GDGCHC 1 cut(s) 563
MluCI AATT 1 cut(s) 523
MmeI TCCRAC 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 564, 614
Mva1269I GAATGC 2 cut(s) 399, 458
MvaI CCWGG 2 cut(s) 564, 614
MwoI GCNNNNNNNGC 1 cut(s) 558
NlaIII CATG 3 cut(s) 43, 458, 542
NlaIV GGNNCC 2 cut(s) 142, 300
NsiI ATGCAT 1 cut(s) 460
PagI TCATGA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 319
PctI GAATGC 2 cut(s) 399, 458
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 161, 178, 323
PkrI GCNGC 3 cut(s) 235, 259, 429
Psp6I CCWGG 2 cut(s) 562, 612
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 2 cut(s) 562, 612
PspN4I GGNNCC 2 cut(s) 142, 300
PspPI GGNCC 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 287
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 3 cut(s) 234, 258, 428
Sau96I GGNCC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 564, 614
SduI GDGCHC 1 cut(s) 563
SfaNI GCATC 3 cut(s) 220, 485, 583
SfcI CTRYAG 1 cut(s) 279
Sse9I AATT 1 cut(s) 523
SsiI CCGC 3 cut(s) 113, 233, 430
SspI AATATT 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 562, 612
StyI CCWWGG 2 cut(s) 225, 359
TaaI ACNGT 1 cut(s) 475
TaqI TCGA 1 cut(s) 62
TasI AATT 1 cut(s) 523
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 3 cut(s) 161, 178, 323
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 4 cut(s) 343, 469, 478, 491
TseI GCWGC 2 cut(s) 257, 427
TspDTI ATGAA 4 cut(s) 28, 527, 560, 651
TspRI CASTG 4 cut(s) 343, 469, 478, 491
XmnI GAANNNNTTC 1 cut(s) 642
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.