pycom01g09850

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005230
Physical Location & Seq
Forward (+)
10643980 .. 10647527
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1506 bp
ATGTTTATGACTCCGCAACAACCAAAACAAATTCCAAGCTGCCTTTTATCTACAATGGAGCTGTTGGGGAGGAGTCCTATGAAAACACAAGTGATTATTAAACTAGTGCTAGTAGTACTTGTTGTGGTGGCCATGCTAAGAGGGGCTGAGGGCAGAAGGGCAAAGGGCAAAATATTTGAATCGTTTGAGTACACTGCAATGAACTGCAGAGCTCACAGTGCTTCGCTGACTGATTTTGGAGGAGTTGGAGATGGAAAGACATCAAACACAAAGGCTTTTCATGCCGCCGTCAGTCAGCTGAGCCAGTATGAAACACAGGGCGGTGCTCAGCTCTTTGTTCCGGCAGGGAAGTGGCTGACGGGGAGCTTCAACCTCACCAGCCACTTCACTCTCTATCTCCACAAGGATGCTGTTCTCCTCGCTTCTCAAGACATTAATGAATGGCCTGTGCTCAAAGCCCTCCCATCTTACGGTCGAGGAAGAGACGCGCCAGCTGGAAGGTACACCAGTCTTATATGGGGAACAAATCTCACTGATGTCGTTGTTACAGGTGACAATGGCACAATTGATGGGCAGGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTACTTGATCGAAGTCATGTTCTCAACCGATGTCCAAATCTCAAACCTAACTCTCCTAAATTCTCCATCATGGAATGTCCATCCTGTTTATAGCAGTAATATTCTCGTGAAAGGGATTACTATTATAGCTCCAATTTCATCTCCAAACACAGACGGTATCAACCCAGATTCTTGCACCAATACAAGAATTGAAGACTGCTACATAGTCTCTGGGGATGATTGTGTAGCTGTTAAGAGCGGTTGGGATGAGTACGGAATTGCATTTGGAATGCCTACAAAACAACTAGTAGTCCGACGTCTCACATGCATTTCGCCCTACAGCGCCACCATTGCCCTAGGCAGTGAAATGTCCGGCGGGATCCAAGACGTCCGAGCTGAAGACATCGTGGCCATCCATACCGAATCGGGTGTCCGGATCAAGACCGCCGTAGGGCGAGGAGGGTACGTGAAAGACATATACGCGAGGAGATTCACCATGCACACAATGAAATGGGCGTTTTGGATGACCGGCAATTACGGGTCACATGCTGATGACAAATATGATAAAAATGCACTGCCTGAGATCAAAAATATTAACTACAGGGACATGGTGGCCGATAATGTCACAATGGCTGCAAGGTTGGAAGGCATTGCGGACCACCCGTTTACCGGGATTTGCATCTCGAATGTGACGATTGGACTGGCTGTGAAGGCGAAGAAGCAGCCATGGACGTGCACCGACATTGAGGGGATCACGAGCGGCGTGACTCCTCGCCCTTGTGATCTGCTGCCGGATCAGGGTGCTGAGAAAGCCAAAGCTTGTGATTTTCCTGCAGATGATCTGCCGATTGACCGGGTAGAGCTCAAACAGTGTACATATAGGATGAGCTCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

502

Amino Acids

55.08

Weight (kDa)

7.92

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 169 - 452 3.5e-36 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 928, 999
AccBSI CCGCTC 2 cut(s) 867, 1368
AccII CGCG 2 cut(s) 488, 1091
AccIII TCCGGA 1 cut(s) 1041
AciI CCGC 9 cut(s) 14, 285, 321, 625, 867, 984, 1053, 1262, 1368
AclWI GGATC 5 cut(s) 982, 995, 1052, 1367, 1410
AcoI YGGCCR 3 cut(s) 129, 1017, 1221
AcsI RAATTY 2 cut(s) 30, 688
AcuI CTGAAG 1 cut(s) 1026
AcyI GRCGYC 2 cut(s) 925, 996
AfaI GTAC 6 cut(s) 117, 191, 503, 881, 1073, 1483
AfiI CCNNNNNNNGG 4 cut(s) 470, 1059, 1277, 1406
AgsI TTSAA 3 cut(s) 179, 370, 821
AhlI ACTAGT 2 cut(s) 103, 913
AjiI CACGTC 1 cut(s) 1341
Alw21I GWGCWC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
Alw26I GTCTC 3 cut(s) 477, 841, 932
Alw44I GTGCAC 1 cut(s) 1342
AlwI GGATC 5 cut(s) 982, 995, 1052, 1367, 1410
Aor13HI TCCGGA 1 cut(s) 1041
AoxI GGCC 4 cut(s) 129, 443, 1017, 1221
ApaLI GTGCAC 1 cut(s) 1342
ApeKI GCWGC 4 cut(s) 39, 1241, 1330, 1396
ApoI RAATTY 2 cut(s) 30, 688
ArsI GACNNNNNNTTYG 2 cut(s) 1023, 1055
AseI ATTAAT 1 cut(s) 435
AspA2I CCTAGG 1 cut(s) 964
AspLEI GCGC 2 cut(s) 490, 953
AspS9I GGNCC 1 cut(s) 1264
AsuC2I CCSGG 2 cut(s) 1279, 1463
AsuHPI GGTGA 4 cut(s) 367, 563, 590, 1093
AvaII GGWCC 1 cut(s) 1264
AvrII CCTAGG 1 cut(s) 964
BaeGI GKGCMC 1 cut(s) 1346
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BalI TGGCCA 2 cut(s) 131, 1019
BamHI GGATCC 1 cut(s) 987
BanII GRGCYC 3 cut(s) 214, 1473, 1499
BarI GAAGNNNNNNTAC 2 cut(s) 602, 634
BauI CACGAG 2 cut(s) 734, 1363
BbsI GAAGAC 2 cut(s) 828, 1014
Bbv12I GWGCWC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
BbvCI CCTCAGC 1 cut(s) 147
BbvI GCAGC 4 cut(s) 26, 1228, 1342, 1383
BccI CCATC 6 cut(s) 245, 472, 563, 703, 717, 1028
BceAI ACGGC 2 cut(s) 272, 1040
BcnI CCSGG 2 cut(s) 1279, 1463
BcoDI GTCTC 3 cut(s) 477, 841, 932
BcuI ACTAGT 2 cut(s) 103, 913
BfaI CTAG 4 cut(s) 104, 110, 914, 965
BfmI CTRYAG 4 cut(s) 205, 946, 1207, 1440
BfoI RGCGCY 1 cut(s) 954
BisI GCNGC 6 cut(s) 40, 285, 1242, 1331, 1369, 1397
BlnI CCTAGG 1 cut(s) 964
BlpI GCTNAGC 2 cut(s) 299, 327
BlsI GCNGC 6 cut(s) 41, 286, 1243, 1332, 1370, 1398
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 2 cut(s) 1279, 1463
Bme18I GGWCC 1 cut(s) 1264
BmgBI CACGTC 1 cut(s) 1341
BmgT120I GGNCC 1 cut(s) 1264
BmiI GGNNCC 1 cut(s) 989
BmrFI CCNGG 2 cut(s) 1279, 1463
BmsI GCATC 2 cut(s) 397, 1296
BpiI GAAGAC 2 cut(s) 828, 1014
Bpu10I CCTNAGC 1 cut(s) 147
Bpu1102I GCTNAGC 2 cut(s) 299, 327
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 2 cut(s) 1279, 1463
BsaAI YACGTR 1 cut(s) 1075
BsaBI GATNNNNATC 1 cut(s) 1286
BsaHI GRCGYC 2 cut(s) 925, 996
BsaJI CCNNGG 2 cut(s) 964, 1334
BsaWI WCCGGW 1 cut(s) 1041
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 4 cut(s) 470, 1059, 1277, 1406
Bse118I RCCGGY 1 cut(s) 1136
Bse1I ACTGG 3 cut(s) 304, 507, 1314
Bse3DI GCAATG 3 cut(s) 204, 957, 1257
Bse8I GATNNNNATC 1 cut(s) 1286
BseAI TCCGGA 1 cut(s) 1041
BseDI CCNNGG 2 cut(s) 964, 1334
BseGI GGATG 7 cut(s) 412, 709, 850, 880, 1020, 1137, 1497
BseJI GATNNNNATC 1 cut(s) 1286
BseLI CCNNNNNNNGG 4 cut(s) 470, 1059, 1277, 1406
BseMI GCAATG 3 cut(s) 204, 957, 1257
BseMII CTCAG 5 cut(s) 138, 290, 341, 1179, 1404
BseNI ACTGG 3 cut(s) 304, 507, 1314
BseRI GAGGAG 6 cut(s) 85, 255, 407, 1080, 1108, 1368
BseSI GKGCMC 1 cut(s) 1346
BseXI GCAGC 4 cut(s) 26, 1228, 1342, 1383
Bsh1236I CGCG 2 cut(s) 488, 1091
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 4 cut(s) 131, 445, 1019, 1223
BsiEI CGRYCG 1 cut(s) 475
BsiHKAI GWGCWC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
BsiSI CCGG 7 cut(s) 341, 981, 1042, 1137, 1278, 1400, 1462
BslFI GGGAC 1 cut(s) 1226
BslI CCNNNNNNNGG 4 cut(s) 470, 1059, 1277, 1406
BsmAI GTCTC 3 cut(s) 477, 841, 932
BsmBI CGTCTC 2 cut(s) 477, 932
BsmFI GGGAC 1 cut(s) 1226
BsmI GAATGC 1 cut(s) 903
BsnI GGCC 4 cut(s) 131, 445, 1019, 1223
Bsp1286I GDGCHC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
Bsp13I TCCGGA 1 cut(s) 1041
Bsp1407I TGTACA 1 cut(s) 1481
Bsp143I GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
Bsp1720I GCTNAGC 2 cut(s) 299, 327
Bsp19I CCATGG 1 cut(s) 1334
BspACI CCGC 9 cut(s) 14, 285, 321, 625, 867, 984, 1053, 1262, 1368
BspANI GGCC 4 cut(s) 131, 445, 1019, 1223
BspCNI CTCAG 5 cut(s) 139, 291, 340, 1180, 1405
BspEI TCCGGA 1 cut(s) 1041
BspFNI CGCG 2 cut(s) 488, 1091
BspLI GGNNCC 1 cut(s) 989
BspMAI CTGCAG 2 cut(s) 209, 1444
BspPI GGATC 5 cut(s) 982, 995, 1052, 1367, 1410
BsrBI CCGCTC 2 cut(s) 867, 1368
BsrDI GCAATG 3 cut(s) 204, 957, 1257
BsrFI RCCGGY 1 cut(s) 1136
BsrGI TGTACA 1 cut(s) 1481
BsrI ACTGG 3 cut(s) 304, 507, 1314
BssAI RCCGGY 1 cut(s) 1136
BssECI CCNNGG 2 cut(s) 964, 1334
BssMI GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
BssNI GRCGYC 2 cut(s) 925, 996
BssSI CACGAG 2 cut(s) 734, 1363
BssT1I CCWWGG 2 cut(s) 964, 1334
Bst2BI CACGAG 2 cut(s) 734, 1363
Bst4CI ACNGT 5 cut(s) 218, 473, 597, 785, 1479
Bst6I CTCTTC 1 cut(s) 475
BstACI GRCGYC 2 cut(s) 925, 996
BstAUI TGTACA 1 cut(s) 1481
BstBAI YACGTR 1 cut(s) 1075
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 6 cut(s) 137, 147, 299, 327, 1188, 1413
BstDSI CCRYGG 1 cut(s) 1334
BstF5I GGATG 7 cut(s) 412, 709, 850, 880, 1020, 1137, 1497
BstFNI CGCG 2 cut(s) 488, 1091
BstH2I RGCGCY 1 cut(s) 954
BstHHI GCGC 2 cut(s) 490, 953
BstKTI GATC 8 cut(s) 639, 990, 1047, 1194, 1362, 1393, 1405, 1450
BstMAI GTCTC 3 cut(s) 477, 841, 932
BstMBI GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 5 cut(s) 218, 281, 959, 1319, 1418
BstNSI RCATGY 2 cut(s) 936, 1157
BstSCI CCNGG 2 cut(s) 1277, 1461
BstSFI CTRYAG 4 cut(s) 205, 946, 1207, 1440
BstSLI GKGCMC 1 cut(s) 1346
BstUI CGCG 2 cut(s) 488, 1091
BstV1I GCAGC 4 cut(s) 26, 1228, 1342, 1383
BstV2I GAAGAC 2 cut(s) 828, 1014
BstX2I RGATCY 1 cut(s) 987
BstYI RGATCY 1 cut(s) 987
BsuRI GGCC 4 cut(s) 131, 445, 1019, 1223
BtgI CCRYGG 1 cut(s) 1334
BtrI CACGTC 1 cut(s) 1341
BtsCI GGATG 7 cut(s) 412, 709, 850, 880, 1020, 1137, 1497
BtsI GCAGTG 3 cut(s) 192, 976, 1181
BtsIMutI CAGTG 6 cut(s) 192, 223, 531, 976, 1181, 1484
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 2 cut(s) 490, 953
Cfr10I RCCGGY 1 cut(s) 1136
Cfr13I GGNCC 1 cut(s) 1264
CseI GACGC 1 cut(s) 494
Csp6I GTAC 6 cut(s) 116, 190, 502, 880, 1072, 1482
CviAII CATG 9 cut(s) 133, 281, 646, 699, 933, 1105, 1154, 1216, 1335
CviQI GTAC 6 cut(s) 116, 190, 502, 880, 1072, 1482
DdeI CTNAG 6 cut(s) 137, 147, 299, 327, 1188, 1413
DpnI GATC 8 cut(s) 638, 989, 1046, 1193, 1361, 1392, 1404, 1449
DpnII GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
EaeI YGGCCR 3 cut(s) 129, 1017, 1221
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
Ecl136II GAGCTC 3 cut(s) 212, 1471, 1497
Eco130I CCWWGG 2 cut(s) 964, 1334
Eco24I GRGCYC 3 cut(s) 214, 1473, 1499
Eco47I GGWCC 1 cut(s) 1264
Eco53kI GAGCTC 3 cut(s) 212, 1471, 1497
Eco57I CTGAAG 1 cut(s) 1026
EcoICRI GAGCTC 3 cut(s) 212, 1471, 1497
EcoT14I CCWWGG 2 cut(s) 964, 1334
EcoT22I ATGCAT 1 cut(s) 938
EcoT38I GRGCYC 3 cut(s) 214, 1473, 1499
ErhI CCWWGG 2 cut(s) 964, 1334
Esp3I CGTCTC 2 cut(s) 477, 932
FaeI CATG 9 cut(s) 136, 284, 649, 702, 936, 1108, 1157, 1219, 1338
FaqI GGGAC 1 cut(s) 1226
FatI CATG 9 cut(s) 132, 280, 645, 698, 932, 1104, 1153, 1215, 1334
FauI CCCGC 2 cut(s) 632, 977
Fnu4HI GCNGC 6 cut(s) 40, 285, 1242, 1331, 1369, 1397
FokI GGATG 6 cut(s) 419, 696, 857, 887, 1007, 1144
FriOI GRGCYC 3 cut(s) 214, 1473, 1499
Fsp4HI GCNGC 6 cut(s) 40, 285, 1242, 1331, 1369, 1397
FspBI CTAG 4 cut(s) 104, 110, 914, 965
GlaI GCGC 2 cut(s) 489, 952
GluI GCNGC 6 cut(s) 40, 285, 1242, 1331, 1369, 1397
HaeII RGCGCY 1 cut(s) 954
HaeIII GGCC 4 cut(s) 131, 445, 1019, 1223
HapII CCGG 7 cut(s) 341, 981, 1042, 1137, 1278, 1400, 1462
HgaI GACGC 1 cut(s) 494
HhaI GCGC 2 cut(s) 490, 953
Hin1I GRCGYC 2 cut(s) 925, 996
Hin1II CATG 9 cut(s) 136, 284, 649, 702, 936, 1108, 1157, 1219, 1338
Hin6I GCGC 2 cut(s) 488, 951
HinP1I GCGC 2 cut(s) 488, 951
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 7 cut(s) 10, 73, 179, 797, 1031, 1098, 1375
HpaII CCGG 7 cut(s) 341, 981, 1042, 1137, 1278, 1400, 1462
HphI GGTGA 4 cut(s) 367, 563, 590, 1093
Hpy166II GTNNAC 5 cut(s) 192, 504, 1275, 1344, 1482
Hpy188I TCNGA 2 cut(s) 923, 1001
Hpy188III TCNNGA 6 cut(s) 428, 736, 1042, 1048, 1291, 1363
Hpy8I GTNNAC 5 cut(s) 192, 504, 1275, 1344, 1482
Hpy99I CGWCG 1 cut(s) 927
HpyAV CCTTC 4 cut(s) 150, 492, 1247, 1312
HpyCH4III ACNGT 5 cut(s) 218, 473, 597, 785, 1479
HpyCH4IV ACGT 4 cut(s) 925, 996, 1074, 1340
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 281, 959, 1319, 1418
HpyF3I CTNAG 6 cut(s) 137, 147, 299, 327, 1188, 1413
HpySE526I ACGT 4 cut(s) 925, 996, 1074, 1340
Hsp92I GRCGYC 2 cut(s) 925, 996
Hsp92II CATG 9 cut(s) 136, 284, 649, 702, 936, 1108, 1157, 1219, 1338
HspAI GCGC 2 cut(s) 488, 951
Kpn2I TCCGGA 1 cut(s) 1041
Kzo9I GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
LmnI GCTCC 3 cut(s) 58, 363, 763
Lsp1109I GCAGC 4 cut(s) 26, 1228, 1342, 1383
LweI GCATC 2 cut(s) 397, 1296
MaeI CTAG 4 cut(s) 104, 110, 914, 965
MaeII ACGT 4 cut(s) 925, 996, 1074, 1340
MaeIII GTNAC 6 cut(s) 544, 551, 1149, 1231, 1297, 1372
MalI GATC 8 cut(s) 638, 989, 1046, 1193, 1361, 1392, 1404, 1449
MbiI CCGCTC 2 cut(s) 867, 1368
MboI GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
MboII GAAGA 5 cut(s) 492, 619, 833, 1019, 1336
MfeI CAATTG 1 cut(s) 564
MflI RGATCY 1 cut(s) 987
MhlI GDGCHC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
MlsI TGGCCA 2 cut(s) 131, 1019
MluCI AATT 7 cut(s) 30, 564, 688, 762, 816, 885, 1141
MluNI TGGCCA 2 cut(s) 131, 1019
MlyI GAGTC 3 cut(s) 4, 82, 1369
MmeI TCCRAC 3 cut(s) 226, 946, 1230
Mox20I TGGCCA 2 cut(s) 131, 1019
Mph1103I ATGCAT 1 cut(s) 938
MroI TCCGGA 1 cut(s) 1041
MscI TGGCCA 2 cut(s) 131, 1019
MseI TTAA 4 cut(s) 99, 435, 861, 1203
MslI CAYNNNNRTG 4 cut(s) 197, 405, 1158, 1339
Msp20I TGGCCA 2 cut(s) 131, 1019
MspA1I CMGCKG 2 cut(s) 298, 494
MspI CCGG 7 cut(s) 341, 981, 1042, 1137, 1278, 1400, 1462
MspR9I CCNGG 2 cut(s) 1279, 1463
MunI CAATTG 1 cut(s) 564
Mva1269I GAATGC 1 cut(s) 903
MvnI CGCG 2 cut(s) 488, 1091
MwoI GCNNNNNNNGC 5 cut(s) 218, 281, 959, 1319, 1418
NciI CCSGG 2 cut(s) 1279, 1463
NcoI CCATGG 1 cut(s) 1334
NdeII GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
NlaIII CATG 9 cut(s) 136, 284, 649, 702, 936, 1108, 1157, 1219, 1338
NlaIV GGNNCC 1 cut(s) 989
NmuCI GTSAC 5 cut(s) 551, 1149, 1231, 1297, 1372
NsiI ATGCAT 1 cut(s) 938
NspI RCATGY 2 cut(s) 936, 1157
PcsI WCGNNNNNNNCGW 1 cut(s) 1298
PctI GAATGC 1 cut(s) 903
PfeI GAWTC 4 cut(s) 179, 797, 1031, 1098
PkrI GCNGC 6 cut(s) 41, 286, 1243, 1332, 1370, 1398
PleI GAGTC 3 cut(s) 4, 81, 1369
PpsI GAGTC 3 cut(s) 4, 81, 1369
Ppu21I YACGTR 1 cut(s) 1075
PshBI ATTAAT 1 cut(s) 435
Psp124BI GAGCTC 3 cut(s) 214, 1473, 1499
PspN4I GGNNCC 1 cut(s) 989
PspPI GGNCC 1 cut(s) 1264
PstI CTGCAG 2 cut(s) 209, 1444
PsuI RGATCY 1 cut(s) 987
PvuII CAGCTG 2 cut(s) 298, 494
RsaI GTAC 6 cut(s) 117, 191, 503, 881, 1073, 1483
RsaNI GTAC 6 cut(s) 116, 190, 502, 880, 1072, 1482
RseI CAYNNNNRTG 4 cut(s) 197, 405, 1158, 1339
SacI GAGCTC 3 cut(s) 214, 1473, 1499
SaqAI TTAA 4 cut(s) 99, 435, 861, 1203
SatI GCNGC 6 cut(s) 40, 285, 1242, 1331, 1369, 1397
Sau3AI GATC 8 cut(s) 636, 987, 1044, 1191, 1359, 1390, 1402, 1447
Sau96I GGNCC 1 cut(s) 1264
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 3 cut(s) 4, 82, 1369
ScrFI CCNGG 2 cut(s) 1279, 1463
SduI GDGCHC 6 cut(s) 214, 328, 453, 1346, 1473, 1499
SfaNI GCATC 2 cut(s) 397, 1296
SfcI CTRYAG 4 cut(s) 205, 946, 1207, 1440
SinI GGWCC 1 cut(s) 1264
SmiMI CAYNNNNRTG 4 cut(s) 197, 405, 1158, 1339
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SpeI ACTAGT 2 cut(s) 103, 913
Sse9I AATT 7 cut(s) 30, 564, 688, 762, 816, 885, 1141
SsiI CCGC 9 cut(s) 14, 285, 321, 625, 867, 984, 1053, 1262, 1368
SspI AATATT 3 cut(s) 174, 730, 1201
SspMI CTAG 4 cut(s) 104, 110, 914, 965
SstI GAGCTC 3 cut(s) 214, 1473, 1499
StyD4I CCNGG 2 cut(s) 1277, 1461
StyI CCWWGG 2 cut(s) 964, 1334
TaaI ACNGT 5 cut(s) 218, 473, 597, 785, 1479
TaiI ACGT 4 cut(s) 928, 999, 1077, 1343
TaqI TCGA 3 cut(s) 475, 639, 1292
TasI AATT 7 cut(s) 30, 564, 688, 762, 816, 885, 1141
TatI WGTACW 3 cut(s) 115, 189, 1481
TauI GCSGC 2 cut(s) 287, 1371
TfiI GAWTC 4 cut(s) 179, 797, 1031, 1098
Tru1I TTAA 4 cut(s) 99, 435, 861, 1203
Tru9I TTAA 4 cut(s) 99, 435, 861, 1203
TscAI CASTG 6 cut(s) 199, 223, 538, 976, 1188, 1484
TseFI GTSAC 5 cut(s) 551, 1149, 1231, 1297, 1372
TseI GCWGC 4 cut(s) 39, 1241, 1330, 1396
Tsp45I GTSAC 5 cut(s) 551, 1149, 1231, 1297, 1372
TspDTI ATGAA 7 cut(s) 95, 215, 269, 324, 453, 756, 1130
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 6 cut(s) 199, 223, 538, 976, 1188, 1484
VneI GTGCAC 1 cut(s) 1342
VpaK11BI GGWCC 1 cut(s) 1264
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 2 cut(s) 30, 688
XceI RCATGY 2 cut(s) 936, 1157
XmaJI CCTAGG 1 cut(s) 964
XspI CTAG 4 cut(s) 104, 110, 914, 965
ZraI GACGTC 2 cut(s) 926, 997
ZrmI AGTACT 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.