pycom01g10860

Clustered mitochondria protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007431
Physical Location & Seq
Forward (+)
11646643 .. 11656649
10007 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5613 bp
ATGGCGCCCAGAAACAGCCGTGGAAAGGGAAAAGGAGACAAGAAGAAGAAAGAAGAGAAGGTTCTTCCTGTTGTTATGGACATCACTGTGAACCTTCCTGATGAAAACAGTATCGTTTTGAAGGGAATATCGACAGATAGGATTATCGATGTTCATCAGCTGTTATCAGTGAACACAGAAACTTGTAACATTACGAACTTTTCACTATCTCATGAGGTAAGAGGGCAACAATTGAAGGACACGGTGGACGTGTCCGCACTGAAGCCGTGCGTTCTGACGTTGATGGAAGAGGACTACAATGAACAGCGCGCCACGGCGCACGTTAGACGCTTGCTCGACATCGTTGCCTGCACGACGAGCTTCGGAGCGTCGTCTTTGCCGGCGAAGGACCAAAGCTCGAAGCTCGATGCTCCTTCAACTGGCTCCGGTAAAAATGCGCCGGTTGCGCAGGATAAGATCTCCAAGAAATCCAACGCCACGACCGCCGCCGCCGTCAACGCCTCCAAGTCTCAAGTTTCTACCGGAACCGATAAGCGAGACGTGGCGGGGGACTCGGAGACCGAGATGAGCCACTCCTGCCTCAAGCTTGGTTCTTTCTACGACTTCTTCTCTCTCTCCCACCTCACTCCCCCTTTGCAATTTATTCGAAGGGCGGCGAAACGGCAAGTCGATGAGATAGCGGCGGATGATCACATTTTTTCTCTAGAGGTGAAGCTTTGCAATGGGAAGGTGGTTCTTGTTGAAGCTTGCCGAAAGGGGTTTTATAGCGTAGGGAAGCAGCGGGTTCTGTGTCACAACCTCGTTGATTTGCTACGGCAGCTTAGTAGAGCCTTCGACAATGCTTATGATGAGCTCTTGAAAGCATTTTCGGAACGGAATAAGTTTGGGAATCTTCCTTATGGGTTTAGAGCAAACACGTGGCTTGTACCACCTGTTTCAGCGCAGTCACCATCAGTTTTCCCTGCTCTCCCCGTGGAGGATGAAACTTGGGGTGGAAATGGTGGTGGTCTAGGAAGAGATGGGAAATTTGATCTGATCCCTTGGGCTAATGAGTTTTGGCATGTTGCATCCATGCCTTGCAAGACAGCCGAGGAGAGGCAGATTCGTGATAGGAAAGCATTCCTTCTTCACAGCCTATTCGTCGATGTTTCCATTTTTAGAGCCATTAAGGCTGTGCAGCATGTAATGGGAAAACCGGAATTGACTTGTTCAGTTCCAAACAGTGAGATTCTTTACTCCGAGAGTGTAGGGGACTTGAATGTCACAGTTATGAAAGATGTTTCTAATGCAAGCTGTAAGGTAGATACTAAAATTGATGGAATTCAAGCAACTGGAGTGGATAAAGCGAATCTAGCGCAACGAAACCTTCTGAAAGGGATCACTGCAGATGAAAATACTGCCGCCCACGATGTCAACACTCTAGGTGTTGTCAATGTCAGATATTGTGGTTACATTGCAGTTGTCAAGGTTGAGGGGAAAGAAAATAAGAAAGTTGGTTCTCCATCTCAGAGCATTGAATTTGTTGATCAACCCGAAGGTGGTGCCAATGCCCTTAATATCAACAGTTTAAGATTACTACTTCACAAGACAATACCTTCAGAGCAGAATAAACCGGCATCACTTATGCAAACTCTGGAACACGAAGATCGATCAGCCTCGTGTGTTTTTGTTGAGGGGGTGTTAGAAGAGAGTCTTGCTAAGCTTGAGAAAGAGGAGCTAGATTCTGATAATTTTGTGAGATGGGAACTTGGAGCTTGCTGGATACAACACTTGCAAGACCAAAAAAATGCAGACAAAGATAAGAAACCATCAAACGAGAAGGCTAAAAATGAGTTGAAGGTTGAGGGACTTGGGACACCTCTTAAATCCCTTAAGAACAGCAAGAAAAAATCAGATGGAGGCAATACCAAGCTGCAATCTGAAAGCTCAAAGTCTCATGCAGATGGTGTTGTTGCAGAAGTTGAAATTAGTATATCCCCTTCTTTGGAATCAAAGCTTGAGACTAATGCCAAAGAAAATGAGCTTGTGTTGACAGAGATGTTGTCTGATGCTGCCTTTGCTCGACTAAAAGATTCAGAAACTGGACTTCACTTCAAGTCTCTGCAAGAACTGATCGACTTGTCTCAGAAGTATTACTCTGAAGTTGCTCTTCCCAAACTGGTAGCAGATTTTGGTTCCTTGGAACTTTCACCAGTTGATGGTCGGACCTTGACTGATTTCATGCATACTAGAGGTCTTCGGATGCGCTCTCTGGGGCATGTTGTCAAGCTTTCGGAGAAGCTATCCCATGTGCAGTCCCTTTGCATTCATGAGATGATAGTGCGAGCTTTTAAGCACATACTTCAGGCAGTGATTGCTGCCGTTGGTAGCACTGAGAAAATGGCTGTTTCAATTGCTGCTGCATTAAATCTTATGCTGGGGGTTTCGGATAATGAAGAATTAAATAAGTCTTGCAATGTTCATTCTCTTGTGCGGAAATGGCTGGAGGTATTCTTGCAAAAGAGATATGGATGGGATATTAACAGCTTCAACTATGAAGATGTGAGGAGATTTGCGATTCTACGTGGATTATGTCACAAGGTGGGTATTGAGATGGTTCCAAGGGATTTTGATATGGATTCTCCAAATCCGTTCCAAAGTTCAGATATTGTCAGCCTAGTCCCAGTGCATAAGCAAGCTGCTTGCTCTTCGGCAGATGGTAGGCAACTCCTAGAATCATCAAAAACAGCTTTAGATAAGGGAAAACTTGAAGATGCAGTTGCATTTGGCACTAAGGCTCTTGCAAAGCTGGTAGCAGTTTGTGGTCCATACCATCGAATGACAGCAGGAGCTTACAGCCTCCTTGCTGTAGTTTTATATCACACTGGAGACTTTAATCAGGCTACAATTTATCAGCAAAAAGCCCTGGATATCAACGAGAGAGAATTAGGGCTTGACCATCCAGACACCATGAAGAGTTATGGGGATCTTTCTGTGTTCTATTACAGACTTCAACATACGGAGCTGGCTCTCAAGTATGTAAAGCGTGCTCTGTATCTTTTGCATCTCACATGTGGACCATCTCATCCAAACACTGCTGCAACATACATCAATGTGGCTATGATGGAGGAAGGCCTGGGAAATGTACATGTTGCCCTTCGATATCTACACAAAGCTTTGAAGTGTAATCAAAGGTTACTCGGCCCGGACCATATTCAGACAGCAGCAAGTTACCACGCTATTGCAATTGCTCTTTCTTTGATGGAAGCTTACCCTTTAAGCGTTCAGCATGAGCAGACAACTTTGCAAATTCTGCGAGCAAAGCTTGGCCCAGATGACTTACGGACACAAGATGCTGCTGCTTGGCTTGAGTACTTTGAGTCCAAGGCTTTCGAACAGCAAGAAGCTGCAAGAAATGGGACTCGAAAGCCTGATGCATCTATTGCCAGCAAAGGCCACTTAAGTGTATCAGATTTGCTTGACTACATTAGTCCAGCTCATGGTGCCAAAGGGAGAGCGATGGCAGGAAAGAGGAAAAGCTACTTAACAAAGTTGAAGGAAAAATCCATCCAAACTATCAGCTCAGCTAGTTCTGATGAGTCTTCAAAAGAAACCACAAAAGAGGGTTCAGACGAAGAGGGTCAGGAGGCGAATGTACTTGAACCAAGTGATAGAACAGATGTTATCCAGGAGAACAGCTCTCCTCTGGTTGAACCTCAGCACGTTGTGGAAGAGGTTGCAGAGGAAAATTCAAATGTTTTCGATCAGATATCTTCTGAAACATACATTGAAGGTGGTGATGATGGGTGGCAATCAGTTCAAAGACCTAGATCAGCTGGCTCATATGGGCGGCGTCTAAAGCAGCGGCGGGCAACTATTGGGAAGGTCTATAATTACCAGAAAAAGTATGTTGAAAGTGACGTGGACTATTCTTCAGTGAAGAATATTAATCAAAACAGTAGTTATTATCTTGTAAAGAAAAGGCCAACATCTCACGGAAGTTATGCAGACAATCACACTGCAAAACCCTCCCAAGGTACTAAGTTTGGACGGAGAATAGTTAAAGGTGTAACATACAGAGTCAAGTCTATGCCTTTGTCTACCAAAGTAGATACCGAAGAGCCCTCTAACGGTGGCAAAAGTTTGAGTTCTCCATCAGAACCTAGTCAAAACGCTTCACCACATGGAATTGGCCCAGTAAAAAATTCCGTAGTCAGTCTTGGGAAGTCTCCTTCTTACAAGGAAGTAGCATTGGCTCCACCAGGTACTATTGGGAAATTTCAAACCCAGTACAACATTCCTGATAATCAGGAACATGGTGTCCGAGTTCATGAAGAAGAAACAACTGAAGTCAAAGGAGATTCCAAACCAAACACAACTGAGTTAGGAAATGTACTTGAAGAAAAGGACTCTGTGTTGGACTCTGTATTGGTTACGACAGACCACGTACCAGAGGAAACCGGAGCTGCTGAGAAGAAAGGAGAAGTTATTTCAAACGATGCAAAGGAAGATAAGTCTTCACTGATGGTATTTGAGAGTTTGGATGGACACGGATCCAGCGGTGTTAAGATTGATGAAGTGGTAGAAGATAACTTATTGACAGACGGAGTGCCAAAATCTTTGGGTTCTCCCAAGGGAATCTGTGAGACGGATCCATCTGGTACTTGTGAACTGCATGACTCCAACTCTACCATGCAAGGTGTAGACGATGCTGTGAGTTCAGTTGATACTCGGGGACTGCCCAGTAAGAAGCTGTCTGCCTCAGCAGCTCCATTCAATCCCTCACCATCTGCTGCACGTGCTGCTCCGGTTTCCCTGAACATTGCCATACCTTCGGGTGCGGGTAATGTCCCAACTGTTGCACCTTGGCCAGTAAACATGAATCTTCATCCAGGGCCTGGTACTGTCTTACCCACGGTCAATCCAATGTGCTCCTCCCCTCACCACCCATACCATTCTCCTCCTGCGACCCCAAACATCATACAACCATTGCCTTTTATGTACCCTCCTTACAGTCAACCCCAAGTAATCCGAACCAGTGCCTTTCCCGTTCCTACCAGCGGGTTTCATCCAAATCATTTTGCATGGCATAATGTGAACCCCCATGTTCCCGAGTTTGTTCATAGTCCAGTTTGGCCTGGCTGTCATCCAATGGATTTCTCTGCCCCTACACCTGTTGCTGAGCCAATTTCTGAACCGACAGTGGAGCCAAAATTTCACAATGATGATTCTGCTCCAGTTCTGCCAGCGACCATTGACAATTTGGAGGAAACTAAGCAAGAAGTGAGCCTTCTGACGTCAGAGGCAATGAACAATGCAGTTGAAAGTGTAAAGGAAAATGGTCCTTCAAACCTTTGCAGGGTTGAACATGCTCAGAGCGAACCAACTGACAACCCAAATGGAAATGCTGCAAGTAGCGGTGAACGAACAAACGATGGAGAGAAAACCTTCAGCATTTTGATGAGGGGTAGGAGAAACCGAAAGCAGACTCTGCGAATGCCTATCAGTTTGCTCAGTCGACCGTATGGCTCACAGTCCTTCAAAGTCATATGTAACAGAGTAGTCAGAGGGAATGATGCTACCAAAGCCACCAGCTGTTCATCAAGTGAAAATTGCACAGCTACTGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU_N TPR_12 eIF3_p135

Protein Analysis

1871

Amino Acids

204.26

Weight (kDa)

6.22

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 45 - 115 1.6e-10 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 729 - 871 1.8e-23 Translation initiation factor eIF3 subunit 135
TPR_NPHP3 PF24885 914 - 1012 3.8e-07 Nephrocystin-3 TPR domain
TPR_10 PF13374 941 - 982 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 941 - 1011 4.6e-12 Tetratricopeptide repeat
TPR_10 PF13374 1025 - 1065 1.7e-07 Tetratricopeptide repeat
TPR_12 PF13424 1025 - 1099 4.1e-12 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1259
AatII GACGTC 1 cut(s) 5279
Acc16I TGCGCA 1 cut(s) 447
AccB1I GGYRCC 3 cut(s) 4, 1541, 3482
AccB7I CCANNNNNTGG 3 cut(s) 2198, 3479, 4877
AccI GTMKAC 3 cut(s) 4079, 4683, 5497
AccII CGCG 1 cut(s) 309
AclWI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4625, 4638
AcoI YGGCCR 1 cut(s) 4847
AcsI RAATTY 8 cut(s) 1025, 1320, 1517, 3288, 3727, 4183, 4256, 5192
AcuI CTGAAG 7 cut(s) 281, 1581, 2160, 2327, 3897, 4347, 5413
AcvI CACGTG 2 cut(s) 918, 4778
AcyI GRCGYC 3 cut(s) 5, 3832, 5276
AdeI CACNNNGTG 2 cut(s) 2581, 3706
AflII CTTAAG 2 cut(s) 1871, 3439
AflIII ACRYGT 4 cut(s) 249, 915, 3050, 3127
AjiI CACGTC 3 cut(s) 250, 541, 3901
AjnI CCWGG 7 cut(s) 2904, 3114, 3666, 4240, 4870, 4876, 5116
AleI CACNNNNGTG 1 cut(s) 3441
AloI GAACNNNNNNTCC 2 cut(s) 4284, 4316
Alw21I GWGCWC 3 cut(s) 855, 3031, 4913
AlwI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4625, 4638
AlwNI CAGNNNCTG 4 cut(s) 2081, 4877, 5470, 5603
Ama87I CYCGRG 2 cut(s) 4710, 5090
AoxI GGCC 9 cut(s) 3112, 3181, 3307, 3433, 3962, 4171, 4847, 4874, 5114
ApoI RAATTY 8 cut(s) 1025, 1320, 1517, 3288, 3727, 4183, 4256, 5192
AseI ATTAAT 1 cut(s) 3927
Asp700I GAANNNNTTC 2 cut(s) 1118, 5426
AspLEI GCGC 9 cut(s) 7, 309, 311, 319, 439, 448, 943, 1357, 2247
AsuC2I CCSGG 1 cut(s) 3185
AsuHPI GGTGA 8 cut(s) 721, 939, 2181, 3788, 4149, 4755, 4913, 5411
AsuII TTCGAA 2 cut(s) 646, 3372
AvaI CYCGRG 2 cut(s) 4710, 5090
AvaII GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5321
BalI TGGCCA 1 cut(s) 4849
BamHI GGATCC 2 cut(s) 4532, 4630
BanI GGYRCC 3 cut(s) 4, 1541, 3482
BanII GRGCYC 2 cut(s) 855, 4104
BarI GAAGNNNNNNTAC 2 cut(s) 1963, 1995
BauI CACGAG 1 cut(s) 1657
BbrPI CACGTG 2 cut(s) 918, 4778
BbsI GAAGAC 3 cut(s) 2228, 3573, 4488
Bbv12I GWGCWC 3 cut(s) 855, 3031, 4913
BbvCI CCTCAGC 2 cut(s) 3696, 4741
BceAI ACGGC 7 cut(s) 3, 250, 330, 476, 677, 830, 2345
BcgI CGANNNNNNTGC 6 cut(s) 326, 360, 626, 660, 1523, 1557
BciT130I CCWGG 7 cut(s) 2906, 3116, 3668, 4242, 4872, 4878, 5118
BciVI GTATCC 1 cut(s) 1755
BclI TGATCA 2 cut(s) 688, 1525
BcnI CCSGG 1 cut(s) 3185
BfmI CTRYAG 2 cut(s) 1383, 2847
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 2 cut(s) 1871, 3439
BfuI GTATCC 1 cut(s) 1755
BglI GCCNNNNNGGC 1 cut(s) 1169
BglII AGATCT 1 cut(s) 456
BlpI GCTNAGC 3 cut(s) 1698, 3562, 5160
BmcAI AGTACT 1 cut(s) 3353
Bme1390I CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4872, 4878, 5118
Bme18I GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5321
BmeT110I CYCGRG 2 cut(s) 4710, 5090
BmgBI CACGTC 3 cut(s) 250, 541, 3901
BmrFI CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4872, 4878, 5118
BmrI ACTGGG 4 cut(s) 2657, 4169, 4261, 4716
BmuI ACTGGG 4 cut(s) 2657, 4169, 4261, 4716
BpiI GAAGAC 3 cut(s) 2228, 3573, 4488
BplI GAGNNNNNCTC 2 cut(s) 3662, 3694
BpmI CTGGAG 4 cut(s) 1353, 2504, 2886, 5199
Bpu10I CCTNAGC 2 cut(s) 3696, 4741
Bpu1102I GCTNAGC 3 cut(s) 1698, 3562, 5160
Bpu14I TTCGAA 2 cut(s) 646, 3372
BpuEI CTTGAG 6 cut(s) 495, 566, 1724, 2018, 2996, 3368
BpuMI CCSGG 1 cut(s) 3185
Bsa29I ATCGAT 2 cut(s) 147, 1648
BsaAI YACGTR 4 cut(s) 918, 2564, 4426, 4778
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 3 cut(s) 5, 3832, 5276
BsaI GGTCTC 1 cut(s) 551
BsaWI WCCGGW 5 cut(s) 425, 521, 1195, 4439, 4786
Bse118I RCCGGY 3 cut(s) 379, 439, 1612
Bse3DI GCAATG 6 cut(s) 727, 1452, 2461, 4800, 4967, 5292
Bse8I GATNNNNATC 1 cut(s) 153
BseBI CCWGG 7 cut(s) 2906, 3116, 3668, 4242, 4872, 4878, 5118
BseCI ATCGAT 2 cut(s) 147, 1648
BseJI GATNNNNATC 1 cut(s) 153
BseMI GCAATG 6 cut(s) 727, 1452, 2461, 4800, 4967, 5292
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 6 cut(s) 1106, 1727, 2560, 3672, 4903, 4929
BseYI CCCAGC 1 cut(s) 2416
BsgI GTGCAG 4 cut(s) 334, 1196, 2312, 4758
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 2 cut(s) 483, 5501
BshFI GGCC 9 cut(s) 3114, 3183, 3309, 3435, 3964, 4173, 4849, 4876, 5116
BshNI GGYRCC 3 cut(s) 4, 1541, 3482
BshVI ATCGAT 2 cut(s) 147, 1648
BsiEI CGRYCG 2 cut(s) 483, 5501
BsiHKAI GWGCWC 3 cut(s) 855, 3031, 4913
BsiHKCI CYCGRG 2 cut(s) 4710, 5090
BsiSI CCGG 9 cut(s) 380, 426, 440, 522, 1196, 1613, 3185, 4440, 4787
BslFI GGGAC 9 cut(s) 563, 1265, 1860, 1867, 2281, 2645, 3412, 4728, 4814
BsmBI CGTCTC 2 cut(s) 531, 4619
BsmFI GGGAC 9 cut(s) 563, 1265, 1860, 1867, 2281, 2645, 3412, 4728, 4814
BsmI GAATGC 3 cut(s) 1118, 2304, 5481
BsnI GGCC 9 cut(s) 3114, 3183, 3309, 3435, 3964, 4173, 4849, 4876, 5116
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 2 cut(s) 4710, 5090
Bsp119I TTCGAA 2 cut(s) 646, 3372
Bsp1286I GDGCHC 4 cut(s) 855, 3031, 4104, 4913
Bsp1407I TGTACA 1 cut(s) 3124
Bsp1720I GCTNAGC 3 cut(s) 1698, 3562, 5160
BspANI GGCC 9 cut(s) 3114, 3183, 3309, 3435, 3964, 4173, 4849, 4876, 5116
BspDI ATCGAT 2 cut(s) 147, 1648
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 3 cut(s) 211, 2308, 4309
BspMAI CTGCAG 1 cut(s) 1387
BspPI GGATC 7 cut(s) 1030, 1385, 2973, 4527, 4540, 4625, 4638
BspQI GCTCTTC 3 cut(s) 2154, 2692, 4092
BspT104I TTCGAA 2 cut(s) 646, 3372
BspT107I GGYRCC 3 cut(s) 4, 1541, 3482
BspTI CTTAAG 2 cut(s) 1871, 3439
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 6 cut(s) 727, 1452, 2461, 4800, 4967, 5292
BsrFI RCCGGY 3 cut(s) 379, 439, 1612
BsrGI TGTACA 1 cut(s) 3124
BssAI RCCGGY 3 cut(s) 379, 439, 1612
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 3 cut(s) 5, 3832, 5276
BssSI CACGAG 1 cut(s) 1657
BssT1I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4844
Bst2BI CACGAG 1 cut(s) 1657
Bst2UI CCWGG 7 cut(s) 2906, 3116, 3668, 4242, 4872, 4878, 5118
BstACI GRCGYC 3 cut(s) 5, 3832, 5276
BstAFI CTTAAG 2 cut(s) 1871, 3439
BstAPI GCANNNNNTGC 5 cut(s) 2354, 3292, 3422, 4781, 5470
BstAUI TGTACA 1 cut(s) 3124
BstBAI YACGTR 4 cut(s) 918, 2564, 4426, 4778
BstBI TTCGAA 2 cut(s) 646, 3372
BstDSI CCRYGG 4 cut(s) 19, 312, 972, 4893
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 9 cut(s) 7, 309, 311, 319, 439, 448, 943, 1357, 2247
BstMCI CGRYCG 2 cut(s) 483, 5501
BstNI CCWGG 7 cut(s) 2906, 3116, 3668, 4242, 4872, 4878, 5118
BstNSI RCATGY 6 cut(s) 1064, 1184, 2261, 3054, 3131, 5351
BstSCI CCNGG 8 cut(s) 2904, 3114, 3183, 3666, 4240, 4870, 4876, 5116
BstSFI CTRYAG 2 cut(s) 1383, 2847
BstUI CGCG 1 cut(s) 309
BstV2I GAAGAC 3 cut(s) 2228, 3573, 4488
BstX2I RGATCY 4 cut(s) 456, 2965, 4532, 4630
BstYI RGATCY 4 cut(s) 456, 2965, 4532, 4630
Bsu15I ATCGAT 2 cut(s) 147, 1648
BsuI GTATCC 1 cut(s) 1755
BsuRI GGCC 9 cut(s) 3114, 3183, 3309, 3435, 3964, 4173, 4849, 4876, 5116
BsuTUI ATCGAT 2 cut(s) 147, 1648
BtgI CCRYGG 4 cut(s) 19, 312, 972, 4893
BtgZI GCGATG 1 cut(s) 3512
BtrI CACGTC 3 cut(s) 250, 541, 3901
BtsI GCAGTG 4 cut(s) 1380, 2355, 3072, 3996
CaiI CAGNNNCTG 4 cut(s) 2081, 4877, 5470, 5603
CciI TCATGA 3 cut(s) 211, 2308, 4309
CfoI GCGC 9 cut(s) 7, 309, 311, 319, 439, 448, 943, 1357, 2247
Cfr10I RCCGGY 3 cut(s) 379, 439, 1612
ClaI ATCGAT 2 cut(s) 147, 1648
CseI GACGC 3 cut(s) 336, 357, 3821
CsiI ACCWGGT 1 cut(s) 4240
CspCI CAANNNNNGTGG 2 cut(s) 1317, 1352
DinI GGCGCC 1 cut(s) 6
DraIII CACNNNGTG 2 cut(s) 2581, 3706
DrdI GACNNNNNNGTC 1 cut(s) 1259
DseDI GACNNNNNNGTC 1 cut(s) 1259
EaeI YGGCCR 1 cut(s) 4847
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4844
Eco147I AGGCCT 1 cut(s) 3114
Eco24I GRGCYC 2 cut(s) 855, 4104
Eco31I GGTCTC 1 cut(s) 551
Eco32I GATATC 3 cut(s) 2911, 3143, 3750
Eco47I GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5321
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 7 cut(s) 281, 1581, 2160, 2327, 3897, 4347, 5413
Eco72I CACGTG 2 cut(s) 918, 4778
Eco88I CYCGRG 2 cut(s) 4710, 5090
EcoICRI GAGCTC 1 cut(s) 853
EcoO109I RGGNCCY 1 cut(s) 4874
EcoRI GAATTC 1 cut(s) 1320
EcoRII CCWGG 7 cut(s) 2904, 3114, 3666, 4240, 4870, 4876, 5116
EcoRV GATATC 3 cut(s) 2911, 3143, 3750
EcoT14I CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4844
EcoT22I ATGCAT 2 cut(s) 2226, 3418
EcoT38I GRGCYC 2 cut(s) 855, 4104
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4844
Esp3I CGTCTC 2 cut(s) 531, 4619
FalI AAGNNNNNCTT 8 cut(s) 1107, 1139, 1677, 1709, 2133, 2165, 5253, 5285
FaqI GGGAC 9 cut(s) 563, 1265, 1860, 1867, 2281, 2645, 3412, 4728, 4814
FauI CCCGC 5 cut(s) 538, 774, 3840, 4813, 5033
FauNDI CATATG 2 cut(s) 3823, 5528
FbaI TGATCA 2 cut(s) 688, 1525
FblI GTMKAC 3 cut(s) 4079, 4683, 5497
FriOI GRGCYC 2 cut(s) 855, 4104
FspI TGCGCA 1 cut(s) 447
GlaI GCGC 9 cut(s) 6, 308, 310, 318, 438, 447, 942, 1356, 2246
GsaI CCCAGC 1 cut(s) 2420
GsuI CTGGAG 4 cut(s) 1353, 2504, 2886, 5199
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 9 cut(s) 3114, 3183, 3309, 3435, 3964, 4173, 4849, 4876, 5116
HapII CCGG 9 cut(s) 380, 426, 440, 522, 1196, 1613, 3185, 4440, 4787
HgaI GACGC 3 cut(s) 336, 357, 3821
HhaI GCGC 9 cut(s) 7, 309, 311, 319, 439, 448, 943, 1357, 2247
Hin1I GRCGYC 3 cut(s) 5, 3832, 5276
Hin6I GCGC 9 cut(s) 5, 307, 309, 317, 437, 446, 941, 1355, 2245
HinP1I GCGC 9 cut(s) 5, 307, 309, 317, 437, 446, 941, 1355, 2245
HincII GTYRAC 5 cut(s) 496, 1414, 2031, 4997, 5498
HindII GTYRAC 5 cut(s) 496, 1414, 2031, 4997, 5498
HindIII AAGCTT 9 cut(s) 584, 713, 744, 1700, 1994, 2267, 3153, 3246, 3302
HpaII CCGG 9 cut(s) 380, 426, 440, 522, 1196, 1613, 3185, 4440, 4787
HphI GGTGA 8 cut(s) 721, 939, 2181, 3788, 4149, 4755, 4913, 5411
Hpy99I CGWCG 3 cut(s) 358, 373, 1145
Hsp92I GRCGYC 3 cut(s) 5, 3832, 5276
HspAI GCGC 9 cut(s) 5, 307, 309, 317, 437, 446, 941, 1355, 2245
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 2 cut(s) 688, 1525
LguI GCTCTTC 3 cut(s) 2154, 2692, 4092
MabI ACCWGGT 1 cut(s) 4240
MfeI CAATTG 3 cut(s) 230, 2391, 3225
MflI RGATCY 4 cut(s) 456, 2965, 4532, 4630
MhlI GDGCHC 4 cut(s) 855, 3031, 4104, 4913
MlsI TGGCCA 1 cut(s) 4849
MluNI TGGCCA 1 cut(s) 4849
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 4 cut(s) 495, 2183, 4377, 4686
Mox20I TGGCCA 1 cut(s) 4849
Mph1103I ATGCAT 2 cut(s) 2226, 3418
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 2 cut(s) 1118, 5426
MscI TGGCCA 1 cut(s) 4849
MslI CAYNNNNRTG 7 cut(s) 86, 1268, 1941, 3092, 3441, 5202, 5438
Msp20I TGGCCA 1 cut(s) 4849
MspA1I CMGCKG 7 cut(s) 160, 781, 3815, 3844, 4539, 5040, 5574
MspCI CTTAAG 2 cut(s) 1871, 3439
MspI CCGG 9 cut(s) 380, 426, 440, 522, 1196, 1613, 3185, 4440, 4787
MspR9I CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4872, 4878, 5118
MunI CAATTG 3 cut(s) 230, 2391, 3225
Mva1269I GAATGC 3 cut(s) 1118, 2304, 5481
MvaI CCWGG 7 cut(s) 2906, 3116, 3668, 4242, 4872, 4878, 5118
MvnI CGCG 1 cut(s) 309
NaeI GCCGGC 1 cut(s) 381
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 3185
NdeI CATATG 2 cut(s) 3823, 5528
NgoMIV GCCGGC 1 cut(s) 379
NmeAIII GCCGAG 2 cut(s) 1114, 3159
NmuCI GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
NsbI TGCGCA 1 cut(s) 447
NsiI ATGCAT 2 cut(s) 2226, 3418
NspI RCATGY 6 cut(s) 1064, 1184, 2261, 3054, 3131, 5351
NspV TTCGAA 2 cut(s) 646, 3372
OliI CACNNNNGTG 1 cut(s) 3441
PagI TCATGA 3 cut(s) 211, 2308, 4309
PauI GCGCGC 1 cut(s) 307
PceI AGGCCT 1 cut(s) 3114
PciI ACATGT 2 cut(s) 3050, 3127
PciSI GCTCTTC 3 cut(s) 2154, 2692, 4092
PctI GAATGC 3 cut(s) 1118, 2304, 5481
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 2 cut(s) 1118, 5426
PflMI CCANNNNNTGG 3 cut(s) 2198, 3479, 4877
PfoI TCCNGGA 1 cut(s) 3666
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 2 cut(s) 918, 4778
PmlI CACGTG 2 cut(s) 918, 4778
Ppu21I YACGTR 4 cut(s) 918, 2564, 4426, 4778
PscI ACATGT 2 cut(s) 3050, 3127
PshBI ATTAAT 1 cut(s) 3927
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 7 cut(s) 2904, 3114, 3666, 4240, 4870, 4876, 5116
PspCI CACGTG 2 cut(s) 918, 4778
PspFI CCCAGC 1 cut(s) 2416
PspGI CCWGG 7 cut(s) 2904, 3114, 3666, 4240, 4870, 4876, 5116
PstI CTGCAG 1 cut(s) 1387
PstNI CAGNNNCTG 4 cut(s) 2081, 4877, 5470, 5603
PsuI RGATCY 4 cut(s) 456, 2965, 4532, 4630
PteI GCGCGC 1 cut(s) 307
PvuII CAGCTG 3 cut(s) 160, 3815, 5574
RseI CAYNNNNRTG 7 cut(s) 86, 1268, 1941, 3092, 3441, 5202, 5438
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 5496
SapI GCTCTTC 3 cut(s) 2154, 2692, 4092
ScaI AGTACT 1 cut(s) 3353
ScrFI CCNGG 8 cut(s) 2906, 3116, 3185, 3668, 4242, 4872, 4878, 5118
SduI GDGCHC 4 cut(s) 855, 3031, 4104, 4913
SexAI ACCWGGT 1 cut(s) 4240
SfcI CTRYAG 2 cut(s) 1383, 2847
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 2 cut(s) 646, 3372
SinI GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5321
SmiMI CAYNNNNRTG 7 cut(s) 86, 1268, 1941, 3092, 3441, 5202, 5438
SmlI CTYRAG 8 cut(s) 510, 581, 1703, 1871, 1997, 3011, 3347, 3439
SmoI CTYRAG 8 cut(s) 510, 581, 1703, 1871, 1997, 3011, 3347, 3439
SseBI AGGCCT 1 cut(s) 3114
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 3925
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 3114
StyD4I CCNGG 8 cut(s) 2904, 3114, 3183, 3666, 4240, 4870, 4876, 5116
StyI CCWWGG 7 cut(s) 1040, 2178, 2600, 3363, 4012, 4611, 4844
TaqII GACCGA 1 cut(s) 575
TatI WGTACW 5 cut(s) 3124, 3351, 3634, 4269, 4372
TauI GCSGC 7 cut(s) 488, 491, 656, 683, 1403, 3832, 3847
TseFI GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
Tsp45I GTSAC 5 cut(s) 791, 945, 1261, 2573, 3896
Van91I CCANNNNNTGG 3 cut(s) 2198, 3479, 4877
Vha464I CTTAAG 2 cut(s) 1871, 3439
VpaK11BI GGWCC 6 cut(s) 388, 2205, 2804, 3056, 3187, 5321
VspI ATTAAT 1 cut(s) 3927
XapI RAATTY 8 cut(s) 1025, 1320, 1517, 3288, 3727, 4183, 4256, 5192
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 6 cut(s) 1064, 1184, 2261, 3054, 3131, 5351
XcmI CCANNNNNNNNNTGG 1 cut(s) 5239
XmiI GTMKAC 3 cut(s) 4079, 4683, 5497
XmnI GAANNNNTTC 2 cut(s) 1118, 5426
ZraI GACGTC 1 cut(s) 5277
ZrmI AGTACT 1 cut(s) 3353
Zsp2I ATGCAT 2 cut(s) 2226, 3418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.