pycom01g23910

Ribonuclease P MRP protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007354
Physical Location & Seq
Reverse (-)
21052482 .. 21053269
788 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23910.1

Sequence Viewer

Length: 465 bp
ATGGTGGGATTTAAGAACAGGTATATGGTAGTGGAGGTTATCTTGGATCCTAATAGAGAACTGGTGAGAAACGATCCTGTTATCATTACCTCGTACAATGTCACGAAAGCTATCCGAGACAGCATTCTGGTAAATTTTGGGGAGTGCGGTCTGGCTTCGTCACTTGGATCATTCCAAGTCAAGTATGTGAATGACATTACGAGAATGTGTATCATTAGAGCTTCAAGGGATGAATATCAGAAAGTGTGGGCTGCAATTACCATGGTCAGGAGCATTGCAGATTGCCCGGTTCTATTTAATTTGTTGGACCTAAGCAGAAGTATCAAGGCTTTTAAAGAAACCGCATTGAAGTGTGATGAATCAAAATTTGAACAGCACAAGCTGCTTGTTGGATCTAATCTGTCGGCTCAAGATACTCAAAAAATGCAGTACTATCTTGACAAGATCAAAGGCCTGGAGCACTAA

Protein Analysis

155

Amino Acids

17.59

Weight (kDa)

8.65

Isoelectric Point (pI)

27.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_P_Rpp14 PF01900 7 - 115 2.4e-28 Rpp14/Pop5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 147, 342
AclWI GGATC 5 cut(s) 41, 54, 68, 175, 400
AcsI RAATTY 2 cut(s) 133, 365
AfaI GTAC 2 cut(s) 95, 431
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 3 cut(s) 225, 349, 371
AjnI CCWGG 1 cut(s) 453
AluBI AGCT 3 cut(s) 110, 221, 382
AluI AGCT 3 cut(s) 110, 221, 382
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 5 cut(s) 41, 54, 68, 175, 400
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 2 cut(s) 251, 382
ApoI RAATTY 2 cut(s) 133, 365
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 1 cut(s) 307
BamHI GGATCC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 2 cut(s) 238, 369
BciT130I CCWGG 1 cut(s) 455
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 111
BisI GCNGC 2 cut(s) 252, 383
BlsI GCNGC 2 cut(s) 253, 384
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 2 cut(s) 287, 455
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 287, 455
Bpu10I CCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 235
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 238, 369
BshFI GGCC 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 5 cut(s) 46, 73, 167, 392, 444
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 2 cut(s) 147, 342
BspANI GGCC 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 5 cut(s) 41, 54, 68, 175, 400
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 5 cut(s) 46, 73, 167, 392, 444
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 455
BstAPI GCANNNNNTGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 5 cut(s) 49, 76, 170, 395, 447
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 5 cut(s) 46, 73, 167, 392, 444
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstNI CCWGG 1 cut(s) 455
BstSCI CCNGG 2 cut(s) 285, 453
BstV1I GCAGC 2 cut(s) 238, 369
BstX2I RGATCY 2 cut(s) 46, 392
BstYI RGATCY 2 cut(s) 46, 392
BsuRI GGCC 1 cut(s) 453
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 94, 430
CviAII CATG 1 cut(s) 262
CviJI RGCY 8 cut(s) 110, 155, 221, 251, 329, 382, 407, 453
CviKI_1 RGCY 8 cut(s) 110, 155, 221, 251, 329, 382, 407, 453
CviQI GTAC 2 cut(s) 94, 430
DdeI CTNAG 1 cut(s) 311
DpnI GATC 5 cut(s) 48, 75, 169, 394, 446
DpnII GATC 5 cut(s) 46, 73, 167, 392, 444
DraI TTTAAA 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 1 cut(s) 265
FaiI YATR 4 cut(s) 24, 26, 186, 263
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 252, 383
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 252, 383
GluI GCNGC 2 cut(s) 252, 383
HaeIII GGCC 1 cut(s) 453
HapII CCGG 1 cut(s) 287
Hin1II CATG 1 cut(s) 265
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 287
HphI GGTGA 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 116, 240
Hpy188III TCNNGA 4 cut(s) 103, 268, 410, 437
HpyCH4V TGCA 3 cut(s) 254, 278, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 1 cut(s) 311
Hsp92II CATG 1 cut(s) 265
Kzo9I GATC 5 cut(s) 46, 73, 167, 392, 444
LmnI GCTCC 2 cut(s) 270, 457
LpnPI CCDG 8 cut(s) 4, 47, 90, 113, 137, 253, 300, 440
Lsp1109I GCAGC 2 cut(s) 238, 369
MaeIII GTNAC 2 cut(s) 100, 159
MalI GATC 5 cut(s) 48, 75, 169, 394, 446
MboI GATC 5 cut(s) 46, 73, 167, 392, 444
MflI RGATCY 2 cut(s) 46, 392
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 4 cut(s) 133, 255, 298, 365
MmeI TCCRAC 2 cut(s) 285, 370
MnlI CCTC 2 cut(s) 28, 100
MseI TTAA 3 cut(s) 12, 297, 333
MslI CAYNNNNRTG 1 cut(s) 349
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 287, 455
Mva1269I GAATGC 1 cut(s) 123
MvaI CCWGG 1 cut(s) 455
MwoI GCNNNNNNNGC 1 cut(s) 382
NciI CCSGG 1 cut(s) 287
NcoI CCATGG 1 cut(s) 261
NdeII GATC 5 cut(s) 46, 73, 167, 392, 444
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 100, 159
PceI AGGCCT 1 cut(s) 453
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 2 cut(s) 253, 384
Psp6I CCWGG 1 cut(s) 453
PspGI CCWGG 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 307
PsuI RGATCY 2 cut(s) 46, 392
RsaI GTAC 2 cut(s) 95, 431
RsaNI GTAC 2 cut(s) 94, 430
RseI CAYNNNNRTG 1 cut(s) 349
SaqAI TTAA 3 cut(s) 12, 297, 333
SatI GCNGC 2 cut(s) 252, 383
Sau3AI GATC 5 cut(s) 46, 73, 167, 392, 444
Sau96I GGNCC 1 cut(s) 307
ScaI AGTACT 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 287, 455
SduI GDGCHC 1 cut(s) 462
SetI ASST 7 cut(s) 23, 39, 92, 112, 223, 312, 384
SinI GGWCC 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
Sse9I AATT 4 cut(s) 133, 255, 298, 365
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 2 cut(s) 147, 342
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 2 cut(s) 285, 453
StyI CCWWGG 1 cut(s) 261
TasI AATT 4 cut(s) 133, 255, 298, 365
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 12, 297, 333
Tru9I TTAA 3 cut(s) 12, 297, 333
TseFI GTSAC 2 cut(s) 100, 159
TseI GCWGC 2 cut(s) 251, 382
Tsp45I GTSAC 2 cut(s) 100, 159
TspDTI ATGAA 2 cut(s) 246, 372
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 133, 365
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.