pycom02g01100

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005412
Physical Location & Seq
Forward (+)
616916 .. 618214
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGTCAAAGATCTTTACCAGTTGTTATGATGGCTATATCCGGTTGATGGATGCCGAGAAAGAGGTGTTTGATCTAGTACATTCCAGTGAAGAGCCTATATACTCTATGTCCCAACAATCGAAGGGTCCGAACTGCTTATATTTTGCCGAGGGCAATGGAGGTTTAAGTGTCTGGGATCAGAGAACTGGAAGCTTCTCAAACCAGTGGTCTTTCTATGAATACCGAATCAATATCATTGACTTTAGCTCAGCAAACCCTAACGTCATGGCCACTAGTTCCTCAGACGGAACTGCCTGCATTTGGGATTTGAGAAGTATTGATGCAGATAAAACTTTACGAACAGTTAGCCACAATAGGGCTGTGCATTCTGCTTACTTCTCGCCTTCTGGAGGGTTTCTTGCAACGACAAGTATTGACGATACAGTTGGTATATCGAGTGGAGCTAATTTCGAGGACACATCAATGATATACCATAACAATAATACCAGCAGATGGATTTCTAAGTTCAGGGGAATATGGGGGTGGGACGACGCCTATGTCTTCATTGGCAATGTGAAAAGAGGAGTAGATGTGATCTCCCCAGCTGCAAGAAGAGCAGTGCTCACTTTAGAGAGCCCGCACATGACTGCGATTCCGTGTCGATTTGATGCTCACCCTTACAACGTTGGGATGTTAGCAGGATCCACAGCCGGAGGCCAAGTTTATGTATGGACATTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

241

Amino Acids

26.55

Weight (kDa)

5.44

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 3 - 238 1.5e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 5 - 146 7.9e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 7 - 160 2.2e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 18 - 156 2.5e-11 CDC20/Fizzy WD40 domain
EIF3I PF24805 35 - 133 4.1e-06 EIF3I
Beta-prop_EIPR1 PF23609 50 - 142 9.5e-07 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 55 - 144 1.6e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 56 - 237 4.6e-13 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 74 - 141 1.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 76 - 215 2.8e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 87 - 184 7.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 492
AciI CCGC 1 cut(s) 617
AclI AACGTT 1 cut(s) 663
AclWI GGATC 3 cut(s) 183, 675, 688
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 1 cut(s) 78
AfiI CCNNNNNNNGG 5 cut(s) 46, 300, 355, 389, 492
AhlI ACTAGT 1 cut(s) 272
AluBI AGCT 4 cut(s) 192, 246, 443, 584
AluI AGCT 4 cut(s) 192, 246, 443, 584
Alw21I GWGCWC 1 cut(s) 603
AlwI GGATC 3 cut(s) 183, 675, 688
AoxI GGCC 2 cut(s) 267, 694
ApeKI GCWGC 1 cut(s) 584
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 644
AvaII GGWCC 1 cut(s) 125
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 532
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 571
BccI CCATC 3 cut(s) 23, 40, 486
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 74, 273, 721
BglII AGATCT 1 cut(s) 9
BisI GCNGC 1 cut(s) 585
BlpI GCTNAGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 586
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 2 cut(s) 126, 682
BmsI GCATC 3 cut(s) 40, 310, 637
BpiI GAAGAC 1 cut(s) 532
BplI GAGNNNNNCTC 2 cut(s) 585, 617
BpmI CTGGAG 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 4 cut(s) 555, 585, 684, 714
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 300, 355, 389, 492
Bse1I ACTGG 4 cut(s) 18, 84, 190, 202
Bse3DI GCAATG 2 cut(s) 160, 556
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 55, 675
BseLI CCNNNNNNNGG 5 cut(s) 46, 300, 355, 389, 492
BseMI GCAATG 2 cut(s) 160, 556
BseMII CTCAG 2 cut(s) 261, 294
BseNI ACTGG 4 cut(s) 18, 84, 190, 202
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 1 cut(s) 571
BseYI CCCAGC 1 cut(s) 580
BshFI GGCC 2 cut(s) 269, 696
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 2 cut(s) 40, 690
BslFI GGGAC 2 cut(s) 94, 539
BslI CCNNNNNNNGG 5 cut(s) 46, 300, 355, 389, 492
BsmFI GGGAC 2 cut(s) 94, 539
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 269, 696
Bsp1286I GDGCHC 2 cut(s) 603, 617
Bsp143I GATC 5 cut(s) 9, 70, 175, 573, 680
Bsp1720I GCTNAGC 1 cut(s) 247
BspACI CCGC 1 cut(s) 617
BspANI GGCC 2 cut(s) 269, 696
BspCNI CTCAG 2 cut(s) 260, 293
BspLI GGNNCC 2 cut(s) 126, 682
BspPI GGATC 3 cut(s) 183, 675, 688
BspQI GCTCTTC 2 cut(s) 84, 586
BsrDI GCAATG 2 cut(s) 160, 556
BsrI ACTGG 4 cut(s) 18, 84, 190, 202
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 5 cut(s) 9, 70, 175, 573, 680
BssNI GRCGYC 1 cut(s) 531
Bst4CI ACNGT 2 cut(s) 343, 424
Bst6I CTCTTC 2 cut(s) 84, 586
BstACI GRCGYC 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 295, 617
BstDEI CTNAG 3 cut(s) 247, 280, 501
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 55, 675
BstKTI GATC 5 cut(s) 12, 73, 178, 576, 683
BstMBI GATC 5 cut(s) 9, 70, 175, 573, 680
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 1 cut(s) 532
BstX2I RGATCY 2 cut(s) 9, 680
BstYI RGATCY 2 cut(s) 9, 680
BsuRI GGCC 2 cut(s) 269, 696
BtsCI GGATG 2 cut(s) 55, 675
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 91, 209, 603
Cac8I GCNNGC 2 cut(s) 295, 617
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 539
Csp6I GTAC 1 cut(s) 77
CviAII CATG 2 cut(s) 265, 622
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 3 cut(s) 247, 280, 501
DpnI GATC 5 cut(s) 11, 72, 177, 575, 682
DpnII GATC 5 cut(s) 9, 70, 175, 573, 680
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 84, 586
EarI CTCTTC 2 cut(s) 84, 586
Eco24I GRGCYC 1 cut(s) 617
Eco47I GGWCC 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 617
FaeI CATG 2 cut(s) 268, 625
FaqI GGGAC 2 cut(s) 94, 539
FatI CATG 2 cut(s) 264, 621
FauI CCCGC 1 cut(s) 624
Fnu4HI GCNGC 1 cut(s) 585
FokI GGATG 2 cut(s) 62, 682
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 1 cut(s) 585
FspBI CTAG 3 cut(s) 74, 273, 721
GluI GCNGC 1 cut(s) 585
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 2 cut(s) 269, 696
HapII CCGG 2 cut(s) 40, 690
HgaI GACGC 1 cut(s) 539
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 2 cut(s) 268, 625
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 2 cut(s) 225, 631
HpaII CCGG 2 cut(s) 40, 690
HphI GGTGA 1 cut(s) 644
Hpy188I TCNGA 3 cut(s) 129, 180, 283
Hpy188III TCNNGA 1 cut(s) 387
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 2 cut(s) 115, 393
HpyCH4III ACNGT 2 cut(s) 343, 424
HpyCH4IV ACGT 2 cut(s) 261, 663
HpyCH4V TGCA 5 cut(s) 297, 323, 364, 401, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 3 cut(s) 247, 280, 501
HpySE526I ACGT 2 cut(s) 261, 663
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 2 cut(s) 268, 625
Kzo9I GATC 5 cut(s) 9, 70, 175, 573, 680
LguI GCTCTTC 2 cut(s) 84, 586
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 3 cut(s) 40, 310, 637
MaeI CTAG 3 cut(s) 74, 273, 721
MaeII ACGT 2 cut(s) 261, 663
MalI GATC 5 cut(s) 11, 72, 177, 575, 682
MboI GATC 5 cut(s) 9, 70, 175, 573, 680
MboII GAAGA 3 cut(s) 101, 532, 603
MflI RGATCY 2 cut(s) 9, 680
MhlI GDGCHC 2 cut(s) 603, 617
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 269
MnlI CCTC 8 cut(s) 55, 142, 152, 289, 383, 445, 554, 686
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 2 cut(s) 84, 461
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 40, 690
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 1 cut(s) 593
NdeII GATC 5 cut(s) 9, 70, 175, 573, 680
NlaIII CATG 2 cut(s) 268, 625
NlaIV GGNNCC 2 cut(s) 126, 682
NmeAIII GCCGAG 2 cut(s) 79, 172
PciSI GCTCTTC 2 cut(s) 84, 586
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 225, 631
PflMI CCANNNNNTGG 1 cut(s) 492
PkrI GCNGC 1 cut(s) 586
Psp1406I AACGTT 1 cut(s) 663
PspFI CCCAGC 1 cut(s) 580
PspN4I GGNNCC 2 cut(s) 126, 682
PspPI GGNCC 1 cut(s) 125
PsuI RGATCY 2 cut(s) 9, 680
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
RseI CAYNNNNRTG 2 cut(s) 84, 461
SapI GCTCTTC 2 cut(s) 84, 586
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 1 cut(s) 585
Sau3AI GATC 5 cut(s) 9, 70, 175, 573, 680
Sau96I GGNCC 1 cut(s) 125
SduI GDGCHC 2 cut(s) 603, 617
SetI ASST 8 cut(s) 66, 163, 194, 248, 264, 445, 586, 666
SfaNI GCATC 3 cut(s) 40, 310, 637
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 84, 461
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 1 cut(s) 445
SsiI CCGC 1 cut(s) 617
SspMI CTAG 3 cut(s) 74, 273, 721
TaaI ACNGT 2 cut(s) 343, 424
TaiI ACGT 2 cut(s) 264, 666
TaqI TCGA 4 cut(s) 119, 434, 450, 640
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 76
TfiI GAWTC 2 cut(s) 225, 631
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 3 cut(s) 91, 209, 603
TseI GCWGC 1 cut(s) 584
TspDTI ATGAA 2 cut(s) 231, 532
TspGWI ACGGA 2 cut(s) 300, 624
TspRI CASTG 3 cut(s) 91, 209, 603
Van91I CCANNNNNTGG 1 cut(s) 492
VpaK11BI GGWCC 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 387
XspI CTAG 3 cut(s) 74, 273, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.