Rh2AG017500

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005412
Physical Location & Seq
Forward (+)
1346530 .. 1349330
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG017500.1

Sequence Viewer

Length: 1464 bp
ATGGCGATTCGGAAAATCACAGACTATGAGCGCAAGAGGCTCGAGAACATTGGTCGAAACGAGGAATTGATGGCTTCCCTGAAGCTCCAATCCATGGCCACCCAACTTTCTGCCTCAACCAAACGCCCCAGGGTTGACTCCAAACGCAAGACACGGGACCCAACTGTTATCAGGCGTTCTCTACGCACCAGAGGATTACCGCCCGTTGCGAAAGGACTAGGTGGTGATGGTGATGATGACTCTGTGGTGAAGACCCTAGAATCTGTAGACCCTGAGGCATCGGCTCGCAAATTGGGTCCTCTTAGTATGAGAGATGTGTATAGCGGAGTATCGGATAGAGCTCTGATTGAGGCCATTTTGGGTGTTGAGAAGAAACCCCAATTGGGTTTGGAAGTTAAAGGTGAATTTGGGGGTTGCAAGGAGGAGAGATCAAGTGGTGCATTTACCTTAGTCGGTGGGGTTAGTTGTGATTTGAGTAAGAAATTAGGAGGAGCTGAGAGTGATTTGAAGGTGGAATCTTTGACTCTGAATGAGGAGAATGTAGCCCGGGTTGTGCCGGGGAATGTCATGCATGTGAGGTTTTTTCCAAGTACTAGTTTGAGAATGGTTGTGGTTGGGGATTATGCTGGGAATTTTGGATTTTGGAATGTTGATTCTAAGGAAGGGGAGGAAGGTGGGATCTGTATGTATCGTCCACATTCTGGTCCCATTTCGGGGATTTCTATTCACCAAGATTGCATGTCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAGTGGTAAGACTATATATTCTCTCGGTCAACAGTCAAACAATGCAAAGTGTTTATACTTCGCTGAGGGTCGTGGAGATGTAAGTATGTTGGACGAGAGAACAGGAAACATCTCAGCCCAGTGGAGTCCGCATGATGATATAATCAATTCCATAGATTTTAACTCAGAGAACTCAAACATCATCACCACTAGTTCAACAGATGGAACTGCCTGCATTTGGGATTTGAGATGTGTTGATGCAGATAAATTCAAAAGTTTGAAAACAATTGGCCACAAAAGGGCTGTGTATTCTGCCTACTTCTCGCCTTCTGGAAGGTCACTAGTGACGACAAGTGTTGATGACAGGGTTGGTATATCAAGTGGAGTTAATTTTGAGGACATATCAATGATATCCCAACACAATAAAGGCGGATGGCTTCCTAAGTTCAACGCAATATGGGGCTGGGATGATGCATATGTCTTCATCGGAAATAAGAAAAGAGGAATCAATGTTATCTCTCACTTTCAAAGGAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAGTTCAGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCAACAGGTAGAGGCGTAGTTAATGTATGGACCTTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

487

Amino Acids

53.37

Weight (kDa)

8.58

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 173 - 408 8.7e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 188 - 407 8.5e-12 Prp19 WD40 domain
WDR55 PF24796 188 - 351 1.5e-09 WDR55
Beta-prop_ACSF4 PF13570 201 - 385 9e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 202 - 392 3.8e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 209 - 389 5.6e-08 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 215 - 466 2e-14 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 239 - 400 3.1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 276 - 408 3e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 286 - 390 3.9e-08 WDR3 first beta-propeller domain
EIF3I PF24805 286 - 378 7e-06 EIF3I
Beta-prop_EIPR1 PF23609 299 - 388 1.3e-06 EIPR1 beta-propeller
Beta-prop_WDR5 PF25175 301 - 484 2.1e-11 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 312 - 384 2e-06 RIG second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1401
AccB7I CCANNNNNTGG 2 cut(s) 94, 701
AccI GTMKAC 1 cut(s) 267
AciI CCGC 4 cut(s) 200, 324, 950, 1230
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 2 cut(s) 96, 1090
AcsI RAATTY 3 cut(s) 404, 631, 1067
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 592
AfiI CCNNNNNNNGG 8 cut(s) 94, 383, 701, 713, 714, 784, 1038, 1099
AgsI TTSAA 7 cut(s) 508, 822, 1017, 1072, 1081, 1249, 1328
AhlI ACTAGT 4 cut(s) 593, 755, 1010, 1141
AjnI CCWGG 1 cut(s) 128
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 3 cut(s) 85, 341, 494
AluI AGCT 3 cut(s) 85, 341, 494
Alw21I GWGCWC 1 cut(s) 343
AlwI GGATC 1 cut(s) 686
Ama87I CYCGRG 2 cut(s) 41, 546
AoxI GGCC 3 cut(s) 96, 351, 1090
ApeKI GCWGC 1 cut(s) 1388
ApoI RAATTY 3 cut(s) 404, 631, 1067
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 4 cut(s) 157, 296, 704, 1452
AsuC2I CCSGG 3 cut(s) 547, 548, 558
AsuHPI GGTGA 7 cut(s) 236, 242, 259, 413, 719, 997, 1385
AsuNHI GCTAGC 1 cut(s) 1414
AvaI CYCGRG 2 cut(s) 41, 546
AvaII GGWCC 4 cut(s) 157, 296, 704, 1452
AxyI CCTNAGG 1 cut(s) 273
BalI TGGCCA 2 cut(s) 98, 1092
BanII GRGCYC 2 cut(s) 343, 1358
BbsI GAAGAC 2 cut(s) 257, 1273
Bbv12I GWGCWC 1 cut(s) 343
BbvCI CCTCAGC 1 cut(s) 885
BbvI GCAGC 1 cut(s) 1375
BccI CCATC 6 cut(s) 64, 221, 761, 778, 1016, 1227
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 1 cut(s) 130
BcnI CCSGG 3 cut(s) 547, 548, 558
BcuI ACTAGT 4 cut(s) 593, 755, 1010, 1141
BfaI CTAG 8 cut(s) 218, 257, 594, 756, 812, 1011, 1142, 1415
BfmI CTRYAG 2 cut(s) 264, 1368
BglII AGATCT 1 cut(s) 747
BisI GCNGC 1 cut(s) 1389
BlsI GCNGC 1 cut(s) 1390
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 4 cut(s) 130, 547, 548, 558
Bme18I GGWCC 4 cut(s) 157, 296, 704, 1452
BmeT110I CYCGRG 2 cut(s) 41, 546
BmgT120I GGNCC 4 cut(s) 157, 296, 704, 1452
BmiI GGNNCC 4 cut(s) 158, 159, 297, 706
BmrFI CCNGG 4 cut(s) 130, 547, 548, 558
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 5 cut(s) 287, 778, 1048, 1261, 1401
BmtI GCTAGC 1 cut(s) 1418
BmuI ACTGGG 1 cut(s) 934
BpiI GAAGAC 2 cut(s) 257, 1273
Bpu10I CCTNAGC 1 cut(s) 885
BpuMI CCSGG 3 cut(s) 547, 548, 558
BsaJI CCNNGG 6 cut(s) 93, 128, 129, 546, 557, 1455
Bsc4I CCNNNNNNNGG 8 cut(s) 94, 383, 701, 713, 714, 784, 1038, 1099
Bse1I ACTGG 1 cut(s) 940
Bse21I CCTNAGG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 6 cut(s) 93, 128, 129, 546, 557, 1455
BseGI GGATG 4 cut(s) 793, 1238, 1273, 1416
BseLI CCNNNNNNNGG 8 cut(s) 94, 383, 701, 713, 714, 784, 1038, 1099
BseMII CTCAG 6 cut(s) 264, 486, 783, 876, 948, 999
BseNI ACTGG 1 cut(s) 940
BseRI GAGGAG 3 cut(s) 437, 504, 548
BseXI GCAGC 1 cut(s) 1375
BseYI CCCAGC 2 cut(s) 626, 1263
BshFI GGCC 3 cut(s) 98, 353, 1092
BsiHKAI GWGCWC 1 cut(s) 343
BsiHKCI CYCGRG 2 cut(s) 41, 546
BsiSI CCGG 2 cut(s) 547, 557
BslFI GGGAC 2 cut(s) 170, 690
BslI CCNNNNNNNGG 8 cut(s) 94, 383, 701, 713, 714, 784, 1038, 1099
BsmFI GGGAC 2 cut(s) 170, 690
BsnI GGCC 3 cut(s) 98, 353, 1092
BsoBI CYCGRG 2 cut(s) 41, 546
Bsp1286I GDGCHC 2 cut(s) 343, 1358
Bsp143I GATC 4 cut(s) 428, 678, 747, 808
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 4 cut(s) 200, 324, 950, 1230
BspANI GGCC 3 cut(s) 98, 353, 1092
BspCNI CTCAG 6 cut(s) 265, 487, 784, 877, 947, 998
BspLI GGNNCC 4 cut(s) 158, 159, 297, 706
BspMAI CTGCAG 1 cut(s) 1372
BspOI GCTAGC 1 cut(s) 1418
BspPI GGATC 1 cut(s) 686
BsrI ACTGG 1 cut(s) 940
BssECI CCNNGG 6 cut(s) 93, 128, 129, 546, 557, 1455
BssMI GATC 4 cut(s) 428, 678, 747, 808
BssT1I CCWWGG 2 cut(s) 93, 1455
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 2 cut(s) 166, 855
BstC8I GCNNGC 3 cut(s) 286, 1033, 1416
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 793, 1238, 1273, 1416
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 4 cut(s) 431, 681, 750, 811
BstMBI GATC 4 cut(s) 428, 678, 747, 808
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 130
BstNSI RCATGY 2 cut(s) 575, 742
BstSCI CCNGG 4 cut(s) 128, 545, 546, 556
BstSFI CTRYAG 2 cut(s) 264, 1368
BstV1I GCAGC 1 cut(s) 1375
BstV2I GAAGAC 2 cut(s) 257, 1273
BstX2I RGATCY 2 cut(s) 678, 747
BstYI RGATCY 2 cut(s) 678, 747
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 3 cut(s) 98, 353, 1092
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 4 cut(s) 793, 1238, 1273, 1416
BtsIMutI CAGTG 2 cut(s) 947, 1382
Cac8I GCNNGC 3 cut(s) 286, 1033, 1416
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 4 cut(s) 157, 296, 704, 1452
Cfr9I CCCGGG 1 cut(s) 546
Csp6I GTAC 1 cut(s) 591
CviAII CATG 5 cut(s) 94, 568, 572, 739, 953
CviQI GTAC 1 cut(s) 591
DpnI GATC 4 cut(s) 430, 680, 749, 810
DpnII GATC 4 cut(s) 428, 678, 747, 808
DraI TTTAAA 1 cut(s) 1350
EaeI YGGCCR 2 cut(s) 96, 1090
EciI GGCGGA 1 cut(s) 1245
Ecl136II GAGCTC 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 93, 1455
Eco24I GRGCYC 2 cut(s) 343, 1358
Eco32I GATATC 1 cut(s) 1212
Eco47I GGWCC 4 cut(s) 157, 296, 704, 1452
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 101
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 41, 546
EcoICRI GAGCTC 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 157, 296
EcoRII CCWGG 1 cut(s) 128
EcoRV GATATC 1 cut(s) 1212
EcoT14I CCWWGG 2 cut(s) 93, 1455
EcoT22I ATGCAT 2 cut(s) 573, 1276
EcoT38I GRGCYC 2 cut(s) 343, 1358
ErhI CCWWGG 2 cut(s) 93, 1455
FaeI CATG 5 cut(s) 97, 571, 575, 742, 956
FaqI GGGAC 2 cut(s) 170, 690
FatI CATG 5 cut(s) 93, 567, 571, 738, 952
FauNDI CATATG 1 cut(s) 1276
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 1 cut(s) 1389
FokI GGATG 4 cut(s) 800, 1245, 1280, 1423
FriOI GRGCYC 2 cut(s) 343, 1358
Fsp4HI GCNGC 1 cut(s) 1389
FspBI CTAG 8 cut(s) 218, 257, 594, 756, 812, 1011, 1142, 1415
GlaI GCGC 1 cut(s) 32
GluI GCNGC 1 cut(s) 1389
GsaI CCCAGC 2 cut(s) 630, 1267
HaeIII GGCC 3 cut(s) 98, 353, 1092
HapII CCGG 2 cut(s) 547, 557
HhaI GCGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 97, 571, 575, 742, 956
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 2 cut(s) 136, 851
HindII GTYRAC 2 cut(s) 136, 851
HinfI GANTC 9 cut(s) 7, 137, 239, 260, 515, 523, 653, 946, 1305
HpaII CCGG 2 cut(s) 547, 557
HphI GGTGA 7 cut(s) 236, 242, 259, 413, 719, 997, 1385
Hpy166II GTNNAC 4 cut(s) 136, 268, 695, 851
Hpy188I TCNGA 7 cut(s) 12, 334, 345, 528, 779, 988, 1289
Hpy188III TCNNGA 2 cut(s) 43, 1131
Hpy8I GTNNAC 4 cut(s) 136, 268, 695, 851
HpyAV CCTTC 5 cut(s) 502, 656, 665, 1128, 1137
HpyCH4III ACNGT 2 cut(s) 166, 855
HpyCH4V TGCA 9 cut(s) 417, 440, 571, 738, 866, 1035, 1061, 1274, 1370
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
Hsp92II CATG 5 cut(s) 97, 571, 575, 742, 956
HspAI GCGC 1 cut(s) 31
KflI GGGWCCC 1 cut(s) 157
Kzo9I GATC 4 cut(s) 428, 678, 747, 808
LmnI GCTCC 3 cut(s) 90, 491, 1421
Lsp1109I GCAGC 1 cut(s) 1375
LweI GCATC 5 cut(s) 287, 778, 1048, 1261, 1401
MaeI CTAG 8 cut(s) 218, 257, 594, 756, 812, 1011, 1142, 1415
MaeIII GTNAC 2 cut(s) 1137, 1144
MalI GATC 4 cut(s) 430, 680, 749, 810
MboI GATC 4 cut(s) 428, 678, 747, 808
MboII GAAGA 3 cut(s) 262, 382, 1273
MfeI CAATTG 2 cut(s) 380, 1086
MflI RGATCY 2 cut(s) 678, 747
MhlI GDGCHC 2 cut(s) 343, 1358
MlsI TGGCCA 2 cut(s) 98, 1092
MluNI TGGCCA 2 cut(s) 98, 1092
MlyI GAGTC 4 cut(s) 131, 233, 517, 955
MmeI TCCRAC 1 cut(s) 891
Mox20I TGGCCA 2 cut(s) 98, 1092
Mph1103I ATGCAT 2 cut(s) 573, 1276
MscI TGGCCA 2 cut(s) 98, 1092
MseI TTAA 5 cut(s) 396, 981, 1188, 1349, 1443
MslI CAYNNNNRTG 2 cut(s) 572, 1205
Msp20I TGGCCA 2 cut(s) 98, 1092
MspI CCGG 2 cut(s) 547, 557
MspR9I CCNGG 4 cut(s) 130, 547, 548, 558
MunI CAATTG 2 cut(s) 380, 1086
MvaI CCWGG 1 cut(s) 130
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 3 cut(s) 547, 548, 558
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 1276
NdeII GATC 4 cut(s) 428, 678, 747, 808
NheI GCTAGC 1 cut(s) 1414
NlaIII CATG 5 cut(s) 97, 571, 575, 742, 956
NlaIV GGNNCC 4 cut(s) 158, 159, 297, 706
NmuCI GTSAC 2 cut(s) 1137, 1144
NsiI ATGCAT 2 cut(s) 573, 1276
NspI RCATGY 2 cut(s) 575, 742
PaeR7I CTCGAG 1 cut(s) 41
PasI CCCWGGG 1 cut(s) 129
PfeI GAWTC 5 cut(s) 7, 260, 515, 653, 1305
PflMI CCANNNNNTGG 2 cut(s) 94, 701
PkrI GCNGC 1 cut(s) 1390
PleI GAGTC 4 cut(s) 131, 233, 517, 954
PpsI GAGTC 4 cut(s) 131, 233, 517, 954
PpuMI RGGWCCY 2 cut(s) 157, 296
PsiI TTATAA 1 cut(s) 1401
Psp124BI GAGCTC 1 cut(s) 343
Psp5II RGGWCCY 2 cut(s) 157, 296
Psp6I CCWGG 1 cut(s) 128
PspFI CCCAGC 2 cut(s) 626, 1263
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 4 cut(s) 158, 159, 297, 706
PspPI GGNCC 4 cut(s) 157, 296, 704, 1452
PspPPI RGGWCCY 2 cut(s) 157, 296
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 2 cut(s) 678, 747
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 2 cut(s) 572, 1205
SacI GAGCTC 1 cut(s) 343
SaqAI TTAA 5 cut(s) 396, 981, 1188, 1349, 1443
SatI GCNGC 1 cut(s) 1389
Sau3AI GATC 4 cut(s) 428, 678, 747, 808
Sau96I GGNCC 4 cut(s) 157, 296, 704, 1452
ScaI AGTACT 1 cut(s) 592
SchI GAGTC 4 cut(s) 131, 233, 517, 955
ScrFI CCNGG 4 cut(s) 130, 547, 548, 558
SduI GDGCHC 2 cut(s) 343, 1358
SfaNI GCATC 5 cut(s) 287, 778, 1048, 1261, 1401
SfcI CTRYAG 2 cut(s) 264, 1368
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 4 cut(s) 157, 296, 704, 1452
SlaI CTCGAG 1 cut(s) 41
SmaI CCCGGG 1 cut(s) 548
SmiMI CAYNNNNRTG 2 cut(s) 572, 1205
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 4 cut(s) 593, 755, 1010, 1141
SsiI CCGC 4 cut(s) 200, 324, 950, 1230
SspMI CTAG 8 cut(s) 218, 257, 594, 756, 812, 1011, 1142, 1415
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 4 cut(s) 128, 545, 546, 556
StyI CCWWGG 2 cut(s) 93, 1455
TaaI ACNGT 2 cut(s) 166, 855
TaqI TCGA 2 cut(s) 42, 55
TaqII GACCGA 1 cut(s) 836
TatI WGTACW 1 cut(s) 590
TfiI GAWTC 5 cut(s) 7, 260, 515, 653, 1305
Tru1I TTAA 5 cut(s) 396, 981, 1188, 1349, 1443
Tru9I TTAA 5 cut(s) 396, 981, 1188, 1349, 1443
TscAI CASTG 2 cut(s) 947, 1382
TseFI GTSAC 2 cut(s) 1137, 1144
TseI GCWGC 1 cut(s) 1388
Tsp45I GTSAC 2 cut(s) 1137, 1144
TspDTI ATGAA 1 cut(s) 1273
TspMI CCCGGG 1 cut(s) 546
TspRI CASTG 2 cut(s) 947, 1382
Van91I CCANNNNNTGG 2 cut(s) 94, 701
VpaK11BI GGWCC 4 cut(s) 157, 296, 704, 1452
XapI RAATTY 3 cut(s) 404, 631, 1067
XceI RCATGY 2 cut(s) 575, 742
XhoI CTCGAG 1 cut(s) 41
XmaI CCCGGG 1 cut(s) 546
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 8 cut(s) 218, 257, 594, 756, 812, 1011, 1142, 1415
ZrmI AGTACT 1 cut(s) 592
Zsp2I ATGCAT 2 cut(s) 573, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.