Rh2BG017000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005412
Physical Location & Seq
Forward (+)
1207608 .. 1210303
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG017000.1

Sequence Viewer

Length: 1500 bp
ATGGCGACTCAGAAACTCACAGACTATGAGCGCAAGAGGTTCGAGAACATTCGCCGAAACGAGGAATTGATGGCTTCCCTGAAGCTCCAATCCATGGCCACCCAACTCTCTGCCTCAACCAAACGCCCCAGGGTTGAGACCAAATCGTACAAGGTCAATTCGGAGAAGCAGCCCAAGACCCAGGACCCAACTGTTATCAGGCGTTCTCTACGCACCAGAGGATTACCACCCGATGCGAAAGGACTAGGTGGTGATGGTGATGGGGATGATGACTCTGTGGTGAAGACCCTAGAATCTGTAGACCCTAAGGCATCGGCTCGCGAATTGGGTCCTCTTAGTATGAGAGATGTGTATAGCGGAGTATCGGATAGAGCTCTGATTGAGGCCATTTTGGGTGTTGAGAAGAAACCCCAATTGGGTTTGGAAGTTAAAGGTGAATTTGGGGGTTGCAAGGAGGACAGATCAAGTGGTGCATTTACCTTAGTTGGTTGTGATTTGAGTAAGAAATTAGAAGGAGGAGGAGGAGTTGAGAGTGATTTGAAGGTGGAGTCTTTGACTCTGAATGAGGAGAATGTAGCCCGAGTTGTGCCGGGGAATGTCATGAATGTGAGATTTTTTCCGAGTACTAGTTTGAGGATGGTTGTGGTTGGGAATAGGGCTGGGAATGTTGGATTTTGGAATGTTGATTCTAAGGAAGGGGAGGAAGGTGGGTTATGTATGTATCACCCACATTCTGGTCCCATTTCAGGGATTTCTGTTCACCAAGATTGCATGCCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAATGGTGAGACTATATATTCTCTCGCTCAACAGTCAAACAATGCAAAGTGTGTATACTTTGCTGAGGGTCGTGGAGGTATAAGTATGTGGGATGAGAGAACAGGAAACATCTCCGCCCAATGGAGTCCGCATGATGATAGAATCAATTCCATAGACTTTAACTCAGAAAATTCAAACATCATCACCACTAGTTCAACAGATGAAACTGCCTGCATTTGGGATTTGAGATGTACTGATGCAGGTAAATTCAAAAGTTTGAAGACAATTGGCCACAAAAAGGCTGTGCATTCTGCCTACTTCTCACCTTCTGGAAGGTCGCTAGTGACGAGAAGTCTTGATGACACAGTTGGTATATCAAGTGGAGTTAATTTTGAGGACATATCAATGATATCCCATGATAATAATACAGGCAGATGGATTCCTAAGTTCAAGGCAATATGGGGCTGGGATGATGCATATGTCTTCATTGGAAATAAGAATAGAGGAATTGATGTTATCTCCCACCTTCAGAAAAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAATTCCGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCTACAGGTGGAGGCCAAGTTTATGTATGGACATTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

499

Amino Acids

54.72

Weight (kDa)

6.77

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 186 - 419 1.2e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 199 - 364 1.6e-12 Prp19 WD40 domain
WDR55 PF24796 199 - 362 2.5e-11 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 213 - 403 1.9e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 222 - 349 5.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 260 - 400 6.6e-07 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 260 - 497 1e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 286 - 415 6.3e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 288 - 401 3.3e-11 WDR3 first beta-propeller domain
EIF3I PF24805 293 - 388 4.7e-07 EIF3I
Beta-prop_EIPR1 PF23609 297 - 400 2.4e-07 EIPR1 beta-propeller
Beta-prop_WDR5 PF25175 312 - 496 2.6e-13 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 322 - 393 6.7e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 329 - 458 1.2e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1437
Acc36I ACCTGC 1 cut(s) 1085
AccB7I CCANNNNNTGG 2 cut(s) 94, 734
AccI GTMKAC 2 cut(s) 300, 909
AccII CGCG 1 cut(s) 321
AciI CCGC 3 cut(s) 357, 969, 983
AcoI YGGCCR 2 cut(s) 96, 1123
AcsI RAATTY 3 cut(s) 437, 1024, 1100
AcuI CTGAAG 2 cut(s) 101, 1346
AfaI GTAC 3 cut(s) 149, 625, 1087
AgsI TTSAA 7 cut(s) 541, 855, 1029, 1050, 1105, 1114, 1285
AhdI GACNNNNNGTC 1 cut(s) 1185
AhlI ACTAGT 3 cut(s) 626, 788, 1043
AjnI CCWGG 2 cut(s) 128, 180
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 3 cut(s) 85, 374, 1460
AluI AGCT 3 cut(s) 85, 374, 1460
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 2 cut(s) 131, 857
Ama87I CYCGRG 1 cut(s) 579
AoxI GGCC 4 cut(s) 96, 384, 1123, 1471
ApeKI GCWGC 2 cut(s) 169, 1424
ApoI RAATTY 3 cut(s) 437, 1024, 1100
Asp700I GAANNNNTTC 1 cut(s) 1000
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 184, 329, 737
AsuC2I CCSGG 1 cut(s) 591
AsuNHI GCTAGC 1 cut(s) 1450
AvaI CYCGRG 1 cut(s) 579
AvaII GGWCC 3 cut(s) 184, 329, 737
AxyI CCTNAGG 1 cut(s) 306
BalI TGGCCA 2 cut(s) 98, 1125
BanII GRGCYC 2 cut(s) 376, 1394
BbsI GAAGAC 3 cut(s) 290, 1121, 1309
Bbv12I GWGCWC 1 cut(s) 376
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 2 cut(s) 181, 1411
BccI CCATC 7 cut(s) 64, 248, 254, 631, 794, 811, 1263
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 2 cut(s) 130, 182
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 2 cut(s) 131, 857
BcuI ACTAGT 3 cut(s) 626, 788, 1043
BfaI CTAG 8 cut(s) 245, 290, 627, 789, 845, 1044, 1175, 1451
BfmI CTRYAG 2 cut(s) 297, 1461
BfuAI ACCTGC 1 cut(s) 1085
BglII AGATCT 1 cut(s) 780
BisI GCNGC 2 cut(s) 170, 1425
BlsI GCNGC 2 cut(s) 171, 1426
BmcAI AGTACT 1 cut(s) 625
Bme1390I CCNGG 3 cut(s) 130, 182, 591
Bme18I GGWCC 3 cut(s) 184, 329, 737
BmeRI GACNNNNNGTC 1 cut(s) 1185
BmeT110I CYCGRG 1 cut(s) 579
BmgT120I GGNCC 3 cut(s) 184, 329, 737
BmiI GGNNCC 3 cut(s) 186, 330, 739
BmrFI CCNGG 3 cut(s) 130, 182, 591
BmsI GCATC 6 cut(s) 223, 320, 811, 1081, 1297, 1437
BmtI GCTAGC 1 cut(s) 1454
BpiI GAAGAC 3 cut(s) 290, 1121, 1309
Bpu10I CCTNAGC 1 cut(s) 918
BpuMI CCSGG 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 5 cut(s) 93, 128, 129, 180, 590
BsaXI ACNNNNNCTCC 4 cut(s) 510, 540, 1461, 1491
Bse21I CCTNAGG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 130, 182
BseDI CCNNGG 5 cut(s) 93, 128, 129, 180, 590
BseGI GGATG 6 cut(s) 271, 642, 826, 952, 1309, 1452
BseMII CTCAG 4 cut(s) 23, 816, 909, 1032
BseRI GAGGAG 4 cut(s) 531, 534, 537, 581
BseXI GCAGC 2 cut(s) 181, 1411
BseYI CCCAGC 2 cut(s) 659, 1299
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 98, 386, 1125, 1473
BsiHKAI GWGCWC 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 723
BsmAI GTCTC 2 cut(s) 131, 857
BsmFI GGGAC 1 cut(s) 723
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 4 cut(s) 98, 386, 1125, 1473
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 376, 1394
Bsp143I GATC 3 cut(s) 461, 780, 841
Bsp19I CCATGG 1 cut(s) 93
Bsp68I TCGCGA 1 cut(s) 321
BspACI CCGC 3 cut(s) 357, 969, 983
BspANI GGCC 4 cut(s) 98, 386, 1125, 1473
BspCNI CTCAG 4 cut(s) 22, 817, 910, 1031
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 600
BspLI GGNNCC 3 cut(s) 186, 330, 739
BspMI ACCTGC 1 cut(s) 1085
BspOI GCTAGC 1 cut(s) 1454
BspTNI GGTCTC 1 cut(s) 131
BssECI CCNNGG 5 cut(s) 93, 128, 129, 180, 590
BssMI GATC 3 cut(s) 461, 780, 841
BssNAI GTATAC 1 cut(s) 910
BssT1I CCWWGG 1 cut(s) 93
Bst1107I GTATAC 1 cut(s) 910
Bst2UI CCWGG 2 cut(s) 130, 182
Bst4CI ACNGT 3 cut(s) 193, 888, 1201
BstC8I GCNNGC 4 cut(s) 319, 773, 1066, 1452
BstDEI CTNAG 9 cut(s) 9, 306, 335, 481, 690, 825, 918, 1018, 1278
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 6 cut(s) 271, 642, 826, 952, 1309, 1452
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 3 cut(s) 464, 783, 844
BstMAI GTCTC 2 cut(s) 131, 857
BstMBI GATC 3 cut(s) 461, 780, 841
BstNI CCWGG 2 cut(s) 130, 182
BstNSI RCATGY 1 cut(s) 775
BstSCI CCNGG 3 cut(s) 128, 180, 589
BstSFI CTRYAG 2 cut(s) 297, 1461
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 181, 1411
BstV2I GAAGAC 3 cut(s) 290, 1121, 1309
BstX2I RGATCY 1 cut(s) 780
BstYI RGATCY 1 cut(s) 780
BstZ17I GTATAC 1 cut(s) 910
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 4 cut(s) 98, 386, 1125, 1473
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 6 cut(s) 271, 642, 826, 952, 1309, 1452
BtuMI TCGCGA 1 cut(s) 321
BveI ACCTGC 1 cut(s) 1085
Cac8I GCNNGC 4 cut(s) 319, 773, 1066, 1452
CciI TCATGA 1 cut(s) 600
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 184, 329, 737
Csp6I GTAC 3 cut(s) 148, 624, 1086
CviAII CATG 5 cut(s) 94, 601, 772, 986, 1250
CviQI GTAC 3 cut(s) 148, 624, 1086
DdeI CTNAG 9 cut(s) 9, 306, 335, 481, 690, 825, 918, 1018, 1278
DpnI GATC 3 cut(s) 463, 782, 843
DpnII GATC 3 cut(s) 461, 780, 841
DraI TTTAAA 1 cut(s) 1386
DriI GACNNNNNGTC 1 cut(s) 1185
EaeI YGGCCR 2 cut(s) 96, 1123
Eam1105I GACNNNNNGTC 1 cut(s) 1185
EciI GGCGGA 1 cut(s) 958
Ecl136II GAGCTC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 376, 1394
Eco31I GGTCTC 1 cut(s) 131
Eco32I GATATC 1 cut(s) 1245
Eco47I GGWCC 3 cut(s) 184, 329, 737
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 101, 1346
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 374
EcoO109I RGGNCCY 2 cut(s) 184, 329
EcoRII CCWGG 2 cut(s) 128, 180
EcoRV GATATC 1 cut(s) 1245
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 1312
EcoT38I GRGCYC 2 cut(s) 376, 1394
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 5 cut(s) 97, 604, 775, 989, 1253
FaqI GGGAC 1 cut(s) 723
FatI CATG 5 cut(s) 93, 600, 771, 985, 1249
FauNDI CATATG 1 cut(s) 1312
FblI GTMKAC 2 cut(s) 300, 909
Fnu4HI GCNGC 2 cut(s) 170, 1425
FokI GGATG 6 cut(s) 278, 649, 833, 959, 1316, 1459
FriOI GRGCYC 2 cut(s) 376, 1394
Fsp4HI GCNGC 2 cut(s) 170, 1425
FspBI CTAG 8 cut(s) 245, 290, 627, 789, 845, 1044, 1175, 1451
GlaI GCGC 1 cut(s) 32
GluI GCNGC 2 cut(s) 170, 1425
GsaI CCCAGC 2 cut(s) 663, 1303
HaeIII GGCC 4 cut(s) 98, 386, 1125, 1473
HapII CCGG 1 cut(s) 590
HhaI GCGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 97, 604, 775, 989, 1253
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HinfI GANTC 9 cut(s) 7, 272, 293, 548, 556, 686, 979, 996, 1273
HpaII CCGG 1 cut(s) 590
Hpy166II GTNNAC 3 cut(s) 301, 760, 910
Hpy188I TCNGA 9 cut(s) 12, 163, 367, 378, 561, 621, 812, 1021, 1365
Hpy188III TCNNGA 5 cut(s) 43, 320, 601, 1164, 1190
Hpy8I GTNNAC 3 cut(s) 301, 760, 910
HpyAV CCTTC 7 cut(s) 506, 535, 689, 698, 1161, 1170, 1370
HpyCH4III ACNGT 3 cut(s) 193, 888, 1201
HpyCH4V TGCA 9 cut(s) 450, 473, 771, 899, 1068, 1094, 1141, 1310, 1406
HpyF3I CTNAG 9 cut(s) 9, 306, 335, 481, 690, 825, 918, 1018, 1278
Hsp92II CATG 5 cut(s) 97, 604, 775, 989, 1253
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 3 cut(s) 461, 780, 841
LmnI GCTCC 2 cut(s) 90, 1457
Lsp1109I GCAGC 2 cut(s) 181, 1411
LweI GCATC 6 cut(s) 223, 320, 811, 1081, 1297, 1437
MaeI CTAG 8 cut(s) 245, 290, 627, 789, 845, 1044, 1175, 1451
MaeIII GTNAC 1 cut(s) 1177
MalI GATC 3 cut(s) 463, 782, 843
MboI GATC 3 cut(s) 461, 780, 841
MboII GAAGA 4 cut(s) 295, 415, 1126, 1309
MfeI CAATTG 2 cut(s) 413, 1119
MflI RGATCY 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 376, 1394
MlsI TGGCCA 2 cut(s) 98, 1125
MluNI TGGCCA 2 cut(s) 98, 1125
MlyI GAGTC 4 cut(s) 266, 550, 557, 988
MmeI TCCRAC 1 cut(s) 649
Mox20I TGGCCA 2 cut(s) 98, 1125
Mph1103I ATGCAT 1 cut(s) 1312
MroXI GAANNNNTTC 1 cut(s) 1000
MscI TGGCCA 2 cut(s) 98, 1125
MseI TTAA 4 cut(s) 429, 1014, 1221, 1385
MslI CAYNNNNRTG 2 cut(s) 605, 1238
Msp20I TGGCCA 2 cut(s) 98, 1125
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 3 cut(s) 130, 182, 591
MunI CAATTG 2 cut(s) 413, 1119
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 2 cut(s) 130, 182
MvnI CGCG 1 cut(s) 321
NciI CCSGG 1 cut(s) 591
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 1312
NdeII GATC 3 cut(s) 461, 780, 841
NheI GCTAGC 1 cut(s) 1450
NlaIII CATG 5 cut(s) 97, 604, 775, 989, 1253
NlaIV GGNNCC 3 cut(s) 186, 330, 739
NmuCI GTSAC 1 cut(s) 1177
NruI TCGCGA 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 1312
NspI RCATGY 1 cut(s) 775
PaeI GCATGC 1 cut(s) 775
PagI TCATGA 1 cut(s) 600
PasI CCCWGGG 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 1178
PctI GAATGC 1 cut(s) 1141
PdmI GAANNNNTTC 1 cut(s) 1000
PfeI GAWTC 4 cut(s) 293, 686, 996, 1273
PflMI CCANNNNNTGG 2 cut(s) 94, 734
PkrI GCNGC 2 cut(s) 171, 1426
PleI GAGTC 4 cut(s) 266, 550, 556, 987
PpsI GAGTC 4 cut(s) 266, 550, 556, 987
PpuMI RGGWCCY 2 cut(s) 184, 329
PsiI TTATAA 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 376
Psp5II RGGWCCY 2 cut(s) 184, 329
Psp6I CCWGG 2 cut(s) 128, 180
PspFI CCCAGC 2 cut(s) 659, 1299
PspGI CCWGG 2 cut(s) 128, 180
PspN4I GGNNCC 3 cut(s) 186, 330, 739
PspPI GGNCC 3 cut(s) 184, 329, 737
PspPPI RGGWCCY 2 cut(s) 184, 329
PsuI RGATCY 1 cut(s) 780
RruI TCGCGA 1 cut(s) 321
RsaI GTAC 3 cut(s) 149, 625, 1087
RsaNI GTAC 3 cut(s) 148, 624, 1086
RseI CAYNNNNRTG 2 cut(s) 605, 1238
SacI GAGCTC 1 cut(s) 376
SaqAI TTAA 4 cut(s) 429, 1014, 1221, 1385
SatI GCNGC 2 cut(s) 170, 1425
Sau3AI GATC 3 cut(s) 461, 780, 841
Sau96I GGNCC 3 cut(s) 184, 329, 737
ScaI AGTACT 1 cut(s) 625
SchI GAGTC 4 cut(s) 266, 550, 557, 988
ScrFI CCNGG 3 cut(s) 130, 182, 591
SduI GDGCHC 2 cut(s) 376, 1394
SfaNI GCATC 6 cut(s) 223, 320, 811, 1081, 1297, 1437
SfcI CTRYAG 2 cut(s) 297, 1461
SinI GGWCC 3 cut(s) 184, 329, 737
SmiMI CAYNNNNRTG 2 cut(s) 605, 1238
SpeI ACTAGT 3 cut(s) 626, 788, 1043
SphI GCATGC 1 cut(s) 775
SsiI CCGC 3 cut(s) 357, 969, 983
SspMI CTAG 8 cut(s) 245, 290, 627, 789, 845, 1044, 1175, 1451
SstI GAGCTC 1 cut(s) 376
StyD4I CCNGG 3 cut(s) 128, 180, 589
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 193, 888, 1201
TaqI TCGA 1 cut(s) 42
TatI WGTACW 2 cut(s) 623, 1085
TfiI GAWTC 4 cut(s) 293, 686, 996, 1273
Tru1I TTAA 4 cut(s) 429, 1014, 1221, 1385
Tru9I TTAA 4 cut(s) 429, 1014, 1221, 1385
TseFI GTSAC 1 cut(s) 1177
TseI GCWGC 2 cut(s) 169, 1424
Tsp45I GTSAC 1 cut(s) 1177
TspDTI ATGAA 3 cut(s) 617, 1071, 1309
TspGWI ACGGA 1 cut(s) 1401
Van91I CCANNNNNTGG 2 cut(s) 94, 734
VpaK11BI GGWCC 3 cut(s) 184, 329, 737
XapI RAATTY 3 cut(s) 437, 1024, 1100
XceI RCATGY 1 cut(s) 775
XmiI GTMKAC 2 cut(s) 300, 909
XmnI GAANNNNTTC 1 cut(s) 1000
XspI CTAG 8 cut(s) 245, 290, 627, 789, 845, 1044, 1175, 1451
ZrmI AGTACT 1 cut(s) 625
Zsp2I ATGCAT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.