Rh2AG017200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005412
Physical Location & Seq
Forward (+)
1329825 .. 1332939
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG017200.1

Sequence Viewer

Length: 1167 bp
ATGAGAGATGTGTATAGCGGAGTATCGGATAGAGCTCTGATTGAGGCCATTTTGGGTGTTGAGAAGAAACCCCAATTGGGTTTGGAAGTTAAAGGTGAATTTGGGGGTTGCAAAGAGGAGAGATCAAGTGGCGCATTTATCTTAGTTGGTGGGGTTAGTTGTGATTTGAGTAAGAAATTAGAAGAAGGAGGAGTTGAGAGTGATTTGAAGGTGGAGTCTTTGACTCTGAATGAGGAGAATGTAGCCCGAGTTGTGCCGGGGAATGTCATGAATGTGAGATTTTTTCCGAGTACTAGTTTGAGGATGGTTGTGGTTGGGAATAAGGCTGGGAATGTTGGATTTTGGAATGTTGATTCTAAGGAAAGGGAGGAAGGTGGGATATGTTTGTATCACCCACATTCTGGTCCCATTTCGGGGATTTCTGTTCACCAAGATTGCATGTCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAGTGGTGAGACTATATATTCTCTCGCTCAACAGTCAAACAATGCAAAGTGTTTATACTTTGCTGAGGGTCGTGGAGGTATAAGTATGTGGGATGAGAGAACAGGAAACATCTCAGCCCAATGGAGTCCGCATGATGATAGAATCAATTCCATAGACTTTAACTCAGAAAATTCAAACATCATCACCACTAGTTCAACAGATGGAACTGCCTGCATTTGGGATTTGAGATGTATTGATGCAGATAAATTCAAAAGTTTGAAGACAATTGGCCACAAAAGGGCTGTGCATTCTGCCTACTTCTCACCTTCTGGAAGGTCGCTAGTGACGACAAGTCTTGATGACACAGTTGGTATATCAAGTGGAGTTAATTTTGAGGACATATCAAGGATATCCCATGACAATAATACAGGCAGATGGATTCCTAAGTTCAAGGCAATATGGGGCTGGGATGATGCATATGTCTTCATCGGAAATAAGAATAGAGGAATCGATGTCATCTCCCACCTTCAAAAAAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAATTCCGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCTACAGGTGGAGGCCAAGTTTATGTATGGACATTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

388

Amino Acids

42.57

Weight (kDa)

6.02

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 86 - 290 1.9e-06 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 88 - 287 2e-16 Prp19 WD40 domain
WDR55 PF24796 88 - 250 4.2e-12 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 101 - 292 1.4e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 111 - 353 9.2e-15 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 148 - 289 1.7e-08 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 148 - 386 1.1e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 171 - 305 2.3e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 178 - 290 2.5e-11 WDR3 first beta-propeller domain
EIF3I PF24805 182 - 278 1.6e-07 EIF3I
Beta-prop_EIPR1 PF23609 194 - 290 2.6e-08 EIPR1 beta-propeller
Beta-prop_WDR5 PF25175 201 - 385 9.1e-15 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 212 - 284 1.5e-06 RIG second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1104
AccB7I CCANNNNNTGG 1 cut(s) 401
AciI CCGC 2 cut(s) 18, 650
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 3 cut(s) 98, 691, 767
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 7 cut(s) 77, 401, 413, 414, 484, 738, 799
AgsI TTSAA 8 cut(s) 208, 522, 696, 717, 772, 781, 952, 1031
AhdI GACNNNNNGTC 1 cut(s) 852
AhlI ACTAGT 3 cut(s) 293, 455, 710
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 2 cut(s) 35, 1127
AluI AGCT 2 cut(s) 35, 1127
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 3 cut(s) 45, 790, 1138
ApeKI GCWGC 1 cut(s) 1091
ApoI RAATTY 3 cut(s) 98, 691, 767
Asp700I GAANNNNTTC 1 cut(s) 667
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 7 cut(s) 107, 383, 419, 539, 697, 816, 1088
AsuNHI GCTAGC 1 cut(s) 1117
AvaI CYCGRG 1 cut(s) 246
AvaII GGWCC 1 cut(s) 404
BalI TGGCCA 1 cut(s) 792
BanII GRGCYC 2 cut(s) 37, 1061
BbsI GAAGAC 2 cut(s) 788, 976
Bbv12I GWGCWC 1 cut(s) 37
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 1078
BccI CCATC 5 cut(s) 298, 461, 478, 716, 930
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 524
BcuI ACTAGT 3 cut(s) 293, 455, 710
BfaI CTAG 6 cut(s) 294, 456, 512, 711, 842, 1118
BfmI CTRYAG 1 cut(s) 1128
BglII AGATCT 1 cut(s) 447
BisI GCNGC 1 cut(s) 1092
BlsI GCNGC 1 cut(s) 1093
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 404
BmeRI GACNNNNNGTC 1 cut(s) 852
BmeT110I CYCGRG 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 4 cut(s) 478, 748, 964, 1104
BmtI GCTAGC 1 cut(s) 1121
BpiI GAAGAC 2 cut(s) 788, 976
Bpu10I CCTNAGC 1 cut(s) 585
BpuMI CCSGG 1 cut(s) 258
Bsa29I ATCGAT 1 cut(s) 1011
BsaJI CCNNGG 1 cut(s) 257
BsaXI ACNNNNNCTCC 2 cut(s) 1128, 1158
Bsc4I CCNNNNNNNGG 7 cut(s) 77, 401, 413, 414, 484, 738, 799
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 5 cut(s) 309, 493, 619, 976, 1119
BseLI CCNNNNNNNGG 7 cut(s) 77, 401, 413, 414, 484, 738, 799
BseMII CTCAG 4 cut(s) 483, 576, 648, 699
BseRI GAGGAG 3 cut(s) 131, 204, 248
BseXI GCAGC 1 cut(s) 1078
BseYI CCCAGC 2 cut(s) 326, 966
BshFI GGCC 3 cut(s) 47, 792, 1140
BshVI ATCGAT 1 cut(s) 1011
BsiHKAI GWGCWC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 7 cut(s) 77, 401, 413, 414, 484, 738, 799
BsmAI GTCTC 1 cut(s) 524
BsmFI GGGAC 1 cut(s) 390
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 3 cut(s) 47, 792, 1140
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 37, 1061
Bsp143I GATC 3 cut(s) 122, 447, 508
BspACI CCGC 2 cut(s) 18, 650
BspANI GGCC 3 cut(s) 47, 792, 1140
BspCNI CTCAG 4 cut(s) 484, 577, 647, 698
BspDI ATCGAT 1 cut(s) 1011
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 406
BspOI GCTAGC 1 cut(s) 1121
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 3 cut(s) 122, 447, 508
Bst4CI ACNGT 2 cut(s) 555, 868
BstC8I GCNNGC 2 cut(s) 733, 1119
BstDEI CTNAG 7 cut(s) 142, 357, 492, 585, 634, 685, 945
BstF5I GGATG 5 cut(s) 309, 493, 619, 976, 1119
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 3 cut(s) 125, 450, 511
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 3 cut(s) 122, 447, 508
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 1128
BstV1I GCAGC 1 cut(s) 1078
BstV2I GAAGAC 2 cut(s) 788, 976
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
Bsu15I ATCGAT 1 cut(s) 1011
BsuRI GGCC 3 cut(s) 47, 792, 1140
BsuTUI ATCGAT 1 cut(s) 1011
BtsCI GGATG 5 cut(s) 309, 493, 619, 976, 1119
Cac8I GCNNGC 2 cut(s) 733, 1119
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 404
ClaI ATCGAT 1 cut(s) 1011
Csp6I GTAC 1 cut(s) 291
CviAII CATG 4 cut(s) 268, 439, 653, 917
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 7 cut(s) 142, 357, 492, 585, 634, 685, 945
DpnI GATC 3 cut(s) 124, 449, 510
DpnII GATC 3 cut(s) 122, 447, 508
DraI TTTAAA 1 cut(s) 1053
DriI GACNNNNNGTC 1 cut(s) 852
EaeI YGGCCR 1 cut(s) 790
Eam1105I GACNNNNNGTC 1 cut(s) 852
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 37, 1061
Eco32I GATATC 1 cut(s) 912
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 246
EcoICRI GAGCTC 1 cut(s) 35
EcoRV GATATC 1 cut(s) 912
EcoT22I ATGCAT 1 cut(s) 979
EcoT38I GRGCYC 2 cut(s) 37, 1061
FaeI CATG 4 cut(s) 271, 442, 656, 920
FaqI GGGAC 1 cut(s) 390
FatI CATG 4 cut(s) 267, 438, 652, 916
FauNDI CATATG 1 cut(s) 979
Fnu4HI GCNGC 1 cut(s) 1092
FokI GGATG 5 cut(s) 316, 500, 626, 983, 1126
FriOI GRGCYC 2 cut(s) 37, 1061
Fsp4HI GCNGC 1 cut(s) 1092
FspBI CTAG 6 cut(s) 294, 456, 512, 711, 842, 1118
GlaI GCGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 1092
GsaI CCCAGC 2 cut(s) 330, 970
HaeIII GGCC 3 cut(s) 47, 792, 1140
HapII CCGG 1 cut(s) 257
HhaI GCGC 1 cut(s) 134
Hin1II CATG 4 cut(s) 271, 442, 656, 920
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 7 cut(s) 215, 223, 353, 646, 663, 940, 1008
HpaII CCGG 1 cut(s) 257
HphI GGTGA 7 cut(s) 107, 383, 419, 539, 697, 816, 1088
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 7 cut(s) 28, 39, 228, 288, 479, 688, 992
Hpy188III TCNNGA 3 cut(s) 268, 831, 857
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 6 cut(s) 179, 202, 365, 828, 837, 1037
HpyCH4III ACNGT 2 cut(s) 555, 868
HpyCH4V TGCA 8 cut(s) 111, 438, 566, 735, 761, 808, 977, 1073
HpyF3I CTNAG 7 cut(s) 142, 357, 492, 585, 634, 685, 945
Hsp92II CATG 4 cut(s) 271, 442, 656, 920
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 3 cut(s) 122, 447, 508
LmnI GCTCC 1 cut(s) 1124
Lsp1109I GCAGC 1 cut(s) 1078
LweI GCATC 4 cut(s) 478, 748, 964, 1104
MaeI CTAG 6 cut(s) 294, 456, 512, 711, 842, 1118
MaeIII GTNAC 1 cut(s) 844
MalI GATC 3 cut(s) 124, 449, 510
MboI GATC 3 cut(s) 122, 447, 508
MboII GAAGA 4 cut(s) 76, 194, 793, 976
MfeI CAATTG 2 cut(s) 74, 786
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 2 cut(s) 37, 1061
MlsI TGGCCA 1 cut(s) 792
MluCI AATT 9 cut(s) 74, 98, 176, 667, 691, 767, 786, 889, 1074
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 3 cut(s) 217, 224, 655
MmeI TCCRAC 1 cut(s) 316
Mox20I TGGCCA 1 cut(s) 792
Mph1103I ATGCAT 1 cut(s) 979
MroXI GAANNNNTTC 1 cut(s) 667
MscI TGGCCA 1 cut(s) 792
MseI TTAA 4 cut(s) 90, 681, 888, 1052
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 792
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 2 cut(s) 74, 786
Mva1269I GAATGC 1 cut(s) 808
NciI CCSGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 979
NdeII GATC 3 cut(s) 122, 447, 508
NheI GCTAGC 1 cut(s) 1117
NlaIII CATG 4 cut(s) 271, 442, 656, 920
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 844
NsiI ATGCAT 1 cut(s) 979
NspI RCATGY 1 cut(s) 442
PagI TCATGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 845
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 667
PfeI GAWTC 4 cut(s) 353, 663, 940, 1008
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 1 cut(s) 1093
PleI GAGTC 3 cut(s) 217, 223, 654
PpsI GAGTC 3 cut(s) 217, 223, 654
PsiI TTATAA 1 cut(s) 1104
Psp124BI GAGCTC 1 cut(s) 37
PspFI CCCAGC 2 cut(s) 326, 966
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 404
PsuI RGATCY 1 cut(s) 447
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 1 cut(s) 272
SacI GAGCTC 1 cut(s) 37
SaqAI TTAA 4 cut(s) 90, 681, 888, 1052
SatI GCNGC 1 cut(s) 1092
Sau3AI GATC 3 cut(s) 122, 447, 508
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 3 cut(s) 217, 224, 655
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 2 cut(s) 37, 1061
SfaNI GCATC 4 cut(s) 478, 748, 964, 1104
SfcI CTRYAG 1 cut(s) 1128
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 272
SpeI ACTAGT 3 cut(s) 293, 455, 710
Sse9I AATT 9 cut(s) 74, 98, 176, 667, 691, 767, 786, 889, 1074
SsiI CCGC 2 cut(s) 18, 650
SspMI CTAG 6 cut(s) 294, 456, 512, 711, 842, 1118
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 555, 868
TaqI TCGA 1 cut(s) 1011
TasI AATT 9 cut(s) 74, 98, 176, 667, 691, 767, 786, 889, 1074
TatI WGTACW 1 cut(s) 290
TfiI GAWTC 4 cut(s) 353, 663, 940, 1008
Tru1I TTAA 4 cut(s) 90, 681, 888, 1052
Tru9I TTAA 4 cut(s) 90, 681, 888, 1052
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 1 cut(s) 1091
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 2 cut(s) 284, 976
TspGWI ACGGA 1 cut(s) 1068
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 3 cut(s) 98, 691, 767
XceI RCATGY 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 667
XspI CTAG 6 cut(s) 294, 456, 512, 711, 842, 1118
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.