Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005412

Physical Location & Seq

Genomic Coordinates Forward (+)

1084003 .. 1097169

Gene Structure 13167 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG018700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGCGACTCAGAAACTCACAGACTATGAGCGCAAGAGGCTCGAGAACATTCGCCGGAACGAGGAATTGATGGCTTCCCTGAAGCTCCAATCCATGGCCACCCAACTCTCTGCCTCAACCAAACGCCCCAGGGTTGAGACTAAATCGTACAAGGTCAATTCGGAGAAGCAGCCCAAGACCCAGGACCCAACTGTTATCAGGCGTTCTCTACGCACCAGAGGATTACCACCCGATGCGAAAGAACTAGGTGGTGATGGTGATGATGACTCTGTGGTGAAGACCCTAGAATCTGTAGACCCTAAGGCATCGGCTCGCAAATTGGGTCCTCTTAGTATGGGAGATGTGTATAGCGGAGAATCGGATAGAGCTCTGATTGAGGCCATTTTGGGTGTTGAGAAGAAACCCCAATTGGGTTTGGAAGTTAAAGGTGAATTTGGGGAGAGATCAAGTGGTGCATTTATCTTAGTTGGTGGGGTTAGTTGTGATTTGAGTAAGAAATTAGAAGGAGTTGAGAGTGATTTGAAGGTGGAGTCTTTGACTCTGAATGAGGAGAATGTAGCCCGAGTTGTGCCGGGGAATGTCATGAATGTGAGATTTTTTCCGAGTACTAGTTTGAGGATGGTTGTGGTTGGGAATAAGGCTGGGAATGTTGGATTTTGGAATGTTGATTCTAAGGAAGGGGAGGAAGGTGGGATATGTATGTATCACCCACATTCTGGTCCCATTTCGGGGATTTCTGTTCACCAAGATTGCATGTCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAGTGGTAAGACTATATATTCTCTCGGTCAACAGTCAAACAATGCAAAGTGTTTATACTTCGCTGAGGGTCGTGGAGATGTAAGTATGTTGGACGAGAGAACAGGAAACATCTCAGCCCAGTGGAGTCCGCATGATGATATAATCAATTCCATAGATTTTAACTCAGAGAACTCAAACATCATCACCACTAGTTCAACAGATGGAACTGCCTGCATTTGGGATTTGAGATGTGTTGATGCAGATAAATTCAAAAGTTTGAAAACAATTGGCCACAAAAGGGCTGTGTATTCTGCCTACTTCTCGCCTTCTGGAAGGTCACTAGTGACGACAAGTGTTGATGACAGGGTTGGTATATCAAGTGGAGTTAATTTTGAGGACATATCAATGATATCCCAACACAATAAAGGCGGATGGCTTCCTAAGTTCAACGCAATATGGGGCTGGGATGATGCATATGTCTTCATCGGAAATAAGAAAAGAGGAATCAATGTTATCTCTCACTTTCAAAGGAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAGTTCAGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCAACAGGTAGAGGCGTAGTTAATGTATGGACCTTGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

492

Amino Acids

54.01

Weight (kDa)

8.0

Isoelectric Point (pI)

43.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Gbeta	PF25391	178 - 413	4.6e-06	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	193 - 412	4.9e-13	Prp19 WD40 domain
WDR55	PF24796	193 - 356	4.2e-10	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 397	1.9e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	207 - 394	1.4e-08	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	215 - 471	2.3e-14	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	244 - 405	2.7e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	280 - 413	1.8e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	290 - 395	2.8e-08	WDR3 first beta-propeller domain
EIF3I	PF24805	291 - 383	8.7e-06	EIF3I
Beta-prop_EIPR1	PF23609	304 - 393	1.3e-06	EIPR1 beta-propeller
Beta-prop_WDR5	PF25175	306 - 489	2e-11	WDR5 beta-propeller domain
Beta-prop_RIG_2nd	PF23775	316 - 389	1.8e-06	RIG second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005412)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80710
fragaria_vesca	FvH4_1g01460 FvH4_1g01460 FvH4_1g01460 FvH4_1g01460
malus_domestica	MD02G1011700.v1.1 MD15G1157400.v1.1
prunus_persica	Prupe.7G257900_v2.0.a1 Prupe.7G257900_v2.0.a1
pyrus_communis	pycom02g01070 pycom02g01100 pycom15g14110
rosa_chinensis	RchiOBHm_Chr2g0086391
rosa_laevigata	RLG00000015754
rosa_multiflora	Rmu_co8091234.1_g000001 Rmu_co8291973.1_g000001 Rmu_sc0005127.1_g000007 Rmu_sc0005127.1_g000009 Rmu_sc0041620.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154620 Rroxscaffold_2G00154640
rosa_rugosa	Rorug01G0467000
rosa_samantha	Rh2AG017200 Rh2AG017500 Rh2BG017000 Rh2BG017200 Rh2CG018000 Rh2DG018700
rosa_wichuraiana	Rw2G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1416
AccB7I	CCANNNNNTGG	2 cut(s)	94, 716
AccI	GTMKAC	1 cut(s)	294
AciI	CCGC	3 cut(s)	351, 965, 1245
AcoI	YGGCCR	2 cut(s)	96, 1105
AcsI	RAATTY	2 cut(s)	431, 1082
AcuI	CTGAAG	1 cut(s)	101
AfaI	GTAC	2 cut(s)	149, 607
AfiI	CCNNNNNNNGG	9 cut(s)	61, 94, 410, 716, 728, 729, 799, 1053, 1114
AgsI	TTSAA	7 cut(s)	523, 837, 1032, 1087, 1096, 1264, 1343
AhlI	ACTAGT	4 cut(s)	608, 770, 1025, 1156
AjnI	CCWGG	2 cut(s)	128, 180
AjuI	GAANNNNNNNTTGG	2 cut(s)	392, 424
AluBI	AGCT	2 cut(s)	85, 368
AluI	AGCT	2 cut(s)	85, 368
Alw21I	GWGCWC	1 cut(s)	370
Alw26I	GTCTC	1 cut(s)	131
Ama87I	CYCGRG	2 cut(s)	41, 561
AoxI	GGCC	3 cut(s)	96, 378, 1105
ApeKI	GCWGC	2 cut(s)	169, 1403
ApoI	RAATTY	2 cut(s)	431, 1082
AspLEI	GCGC	1 cut(s)	33
AspS9I	GGNCC	4 cut(s)	184, 323, 719, 1467
AsuC2I	CCSGG	1 cut(s)	573
AsuHPI	GGTGA	8 cut(s)	263, 269, 286, 440, 698, 734, 1012, 1400
AsuNHI	GCTAGC	1 cut(s)	1429
AvaI	CYCGRG	2 cut(s)	41, 561
AvaII	GGWCC	4 cut(s)	184, 323, 719, 1467
AxyI	CCTNAGG	1 cut(s)	300
BalI	TGGCCA	2 cut(s)	98, 1107
BanII	GRGCYC	2 cut(s)	370, 1373
BbsI	GAAGAC	2 cut(s)	284, 1288
Bbv12I	GWGCWC	1 cut(s)	370
BbvCI	CCTCAGC	1 cut(s)	900
BbvI	GCAGC	2 cut(s)	181, 1390
BccI	CCATC	7 cut(s)	64, 248, 613, 776, 793, 1031, 1242
BcgI	CGANNNNNNTGC	2 cut(s)	22, 56
BciT130I	CCWGG	2 cut(s)	130, 182
BcnI	CCSGG	1 cut(s)	573
BcoDI	GTCTC	1 cut(s)	131
BcuI	ACTAGT	4 cut(s)	608, 770, 1025, 1156
BfaI	CTAG	8 cut(s)	245, 284, 609, 771, 827, 1026, 1157, 1430
BfmI	CTRYAG	2 cut(s)	291, 1383
BglII	AGATCT	1 cut(s)	762
BisI	GCNGC	2 cut(s)	170, 1404
BlsI	GCNGC	2 cut(s)	171, 1405
BmcAI	AGTACT	1 cut(s)	607
Bme1390I	CCNGG	3 cut(s)	130, 182, 573
Bme18I	GGWCC	4 cut(s)	184, 323, 719, 1467
BmeT110I	CYCGRG	2 cut(s)	41, 561
BmgT120I	GGNCC	4 cut(s)	184, 323, 719, 1467
BmiI	GGNNCC	3 cut(s)	186, 324, 721
BmrFI	CCNGG	3 cut(s)	130, 182, 573
BmrI	ACTGGG	1 cut(s)	949
BmsI	GCATC	6 cut(s)	223, 314, 793, 1063, 1276, 1416
BmtI	GCTAGC	1 cut(s)	1433
BmuI	ACTGGG	1 cut(s)	949
BpiI	GAAGAC	2 cut(s)	284, 1288
Bpu10I	CCTNAGC	1 cut(s)	900
BpuMI	CCSGG	1 cut(s)	573
BsaJI	CCNNGG	6 cut(s)	93, 128, 129, 180, 572, 1470
Bsc4I	CCNNNNNNNGG	9 cut(s)	61, 94, 410, 716, 728, 729, 799, 1053, 1114
Bse1I	ACTGG	1 cut(s)	955
Bse21I	CCTNAGG	1 cut(s)	300
BseBI	CCWGG	2 cut(s)	130, 182
BseDI	CCNNGG	6 cut(s)	93, 128, 129, 180, 572, 1470
BseGI	GGATG	5 cut(s)	624, 808, 1253, 1288, 1431
BseLI	CCNNNNNNNGG	9 cut(s)	61, 94, 410, 716, 728, 729, 799, 1053, 1114
BseMII	CTCAG	5 cut(s)	23, 798, 891, 963, 1014
BseNI	ACTGG	1 cut(s)	955
BseRI	GAGGAG	1 cut(s)	563
BseXI	GCAGC	2 cut(s)	181, 1390
BseYI	CCCAGC	2 cut(s)	641, 1278
BshFI	GGCC	3 cut(s)	98, 380, 1107
BsiHKAI	GWGCWC	1 cut(s)	370
BsiHKCI	CYCGRG	2 cut(s)	41, 561
BsiSI	CCGG	2 cut(s)	55, 572
BslFI	GGGAC	1 cut(s)	705
BslI	CCNNNNNNNGG	9 cut(s)	61, 94, 410, 716, 728, 729, 799, 1053, 1114
BsmAI	GTCTC	1 cut(s)	131
BsmFI	GGGAC	1 cut(s)	705
BsnI	GGCC	3 cut(s)	98, 380, 1107
BsoBI	CYCGRG	2 cut(s)	41, 561
Bsp1286I	GDGCHC	2 cut(s)	370, 1373
Bsp143I	GATC	3 cut(s)	443, 762, 823
Bsp19I	CCATGG	1 cut(s)	93
BspACI	CCGC	3 cut(s)	351, 965, 1245
BspANI	GGCC	3 cut(s)	98, 380, 1107
BspCNI	CTCAG	5 cut(s)	22, 799, 892, 962, 1013
BspHI	TCATGA	1 cut(s)	582
BspLI	GGNNCC	3 cut(s)	186, 324, 721
BspMAI	CTGCAG	1 cut(s)	1387
BspOI	GCTAGC	1 cut(s)	1433
BsrI	ACTGG	1 cut(s)	955
BssECI	CCNNGG	6 cut(s)	93, 128, 129, 180, 572, 1470
BssMI	GATC	3 cut(s)	443, 762, 823
BssT1I	CCWWGG	2 cut(s)	93, 1470
Bst2UI	CCWGG	2 cut(s)	130, 182
Bst4CI	ACNGT	2 cut(s)	193, 870
BstC8I	GCNNGC	3 cut(s)	313, 1048, 1431
BstDSI	CCRYGG	1 cut(s)	93
BstF5I	GGATG	5 cut(s)	624, 808, 1253, 1288, 1431
BstHHI	GCGC	1 cut(s)	33
BstKTI	GATC	3 cut(s)	446, 765, 826
BstMAI	GTCTC	1 cut(s)	131
BstMBI	GATC	3 cut(s)	443, 762, 823
BstMWI	GCNNNNNNNGC	1 cut(s)	37
BstNI	CCWGG	2 cut(s)	130, 182
BstNSI	RCATGY	1 cut(s)	757
BstSCI	CCNGG	3 cut(s)	128, 180, 571
BstSFI	CTRYAG	2 cut(s)	291, 1383
BstV1I	GCAGC	2 cut(s)	181, 1390
BstV2I	GAAGAC	2 cut(s)	284, 1288
BstX2I	RGATCY	1 cut(s)	762
BstYI	RGATCY	1 cut(s)	762
Bsu36I	CCTNAGG	1 cut(s)	300
BsuRI	GGCC	3 cut(s)	98, 380, 1107
BtgI	CCRYGG	1 cut(s)	93
BtsCI	GGATG	5 cut(s)	624, 808, 1253, 1288, 1431
BtsIMutI	CAGTG	2 cut(s)	962, 1397
Cac8I	GCNNGC	3 cut(s)	313, 1048, 1431
CciI	TCATGA	1 cut(s)	582
CfoI	GCGC	1 cut(s)	33
Cfr13I	GGNCC	4 cut(s)	184, 323, 719, 1467
Csp6I	GTAC	2 cut(s)	148, 606
CviAII	CATG	4 cut(s)	94, 583, 754, 968
CviQI	GTAC	2 cut(s)	148, 606
DpnI	GATC	3 cut(s)	445, 764, 825
DpnII	GATC	3 cut(s)	443, 762, 823
DraI	TTTAAA	1 cut(s)	1365
EaeI	YGGCCR	2 cut(s)	96, 1105
EciI	GGCGGA	1 cut(s)	1260
Ecl136II	GAGCTC	1 cut(s)	368
Eco130I	CCWWGG	2 cut(s)	93, 1470
Eco24I	GRGCYC	2 cut(s)	370, 1373
Eco32I	GATATC	1 cut(s)	1227
Eco47I	GGWCC	4 cut(s)	184, 323, 719, 1467
Eco53kI	GAGCTC	1 cut(s)	368
Eco57I	CTGAAG	1 cut(s)	101
Eco81I	CCTNAGG	1 cut(s)	300
Eco88I	CYCGRG	2 cut(s)	41, 561
EcoICRI	GAGCTC	1 cut(s)	368
EcoO109I	RGGNCCY	2 cut(s)	184, 323
EcoRII	CCWGG	2 cut(s)	128, 180
EcoRV	GATATC	1 cut(s)	1227
EcoT14I	CCWWGG	2 cut(s)	93, 1470
EcoT22I	ATGCAT	1 cut(s)	1291
EcoT38I	GRGCYC	2 cut(s)	370, 1373
ErhI	CCWWGG	2 cut(s)	93, 1470
FaeI	CATG	4 cut(s)	97, 586, 757, 971
FaqI	GGGAC	1 cut(s)	705
FatI	CATG	4 cut(s)	93, 582, 753, 967
FauNDI	CATATG	1 cut(s)	1291
FblI	GTMKAC	1 cut(s)	294
Fnu4HI	GCNGC	2 cut(s)	170, 1404
FokI	GGATG	5 cut(s)	631, 815, 1260, 1295, 1438
FriOI	GRGCYC	2 cut(s)	370, 1373
Fsp4HI	GCNGC	2 cut(s)	170, 1404
FspBI	CTAG	8 cut(s)	245, 284, 609, 771, 827, 1026, 1157, 1430
GlaI	GCGC	1 cut(s)	32
GluI	GCNGC	2 cut(s)	170, 1404
GsaI	CCCAGC	2 cut(s)	645, 1282
HaeIII	GGCC	3 cut(s)	98, 380, 1107
HapII	CCGG	2 cut(s)	55, 572
HhaI	GCGC	1 cut(s)	33
Hin1II	CATG	4 cut(s)	97, 586, 757, 971
Hin6I	GCGC	1 cut(s)	31
HinP1I	GCGC	1 cut(s)	31
HincII	GTYRAC	1 cut(s)	866
HindII	GTYRAC	1 cut(s)	866
HinfI	GANTC	9 cut(s)	7, 266, 287, 356, 530, 538, 668, 961, 1320
HpaII	CCGG	2 cut(s)	55, 572
HphI	GGTGA	8 cut(s)	263, 269, 286, 440, 698, 734, 1012, 1400
Hpy166II	GTNNAC	3 cut(s)	295, 742, 866
Hpy188I	TCNGA	9 cut(s)	12, 163, 361, 372, 543, 603, 794, 1003, 1304
Hpy188III	TCNNGA	3 cut(s)	43, 583, 1146
Hpy8I	GTNNAC	3 cut(s)	295, 742, 866
HpyAV	CCTTC	6 cut(s)	497, 517, 671, 680, 1143, 1152
HpyCH4III	ACNGT	2 cut(s)	193, 870
HpyCH4V	TGCA	7 cut(s)	455, 753, 881, 1050, 1076, 1289, 1385
HpyF10VI	GCNNNNNNNGC	1 cut(s)	37
Hsp92II	CATG	4 cut(s)	97, 586, 757, 971
HspAI	GCGC	1 cut(s)	31
Kzo9I	GATC	3 cut(s)	443, 762, 823
LmnI	GCTCC	2 cut(s)	90, 1436
Lsp1109I	GCAGC	2 cut(s)	181, 1390
LweI	GCATC	6 cut(s)	223, 314, 793, 1063, 1276, 1416
MaeI	CTAG	8 cut(s)	245, 284, 609, 771, 827, 1026, 1157, 1430
MaeIII	GTNAC	2 cut(s)	1152, 1159
MalI	GATC	3 cut(s)	445, 764, 825
MboI	GATC	3 cut(s)	443, 762, 823
MboII	GAAGA	3 cut(s)	289, 409, 1288
MfeI	CAATTG	2 cut(s)	407, 1101
MflI	RGATCY	1 cut(s)	762
MhlI	GDGCHC	2 cut(s)	370, 1373
MlsI	TGGCCA	2 cut(s)	98, 1107
MluNI	TGGCCA	2 cut(s)	98, 1107
MlyI	GAGTC	4 cut(s)	260, 532, 539, 970
MmeI	TCCRAC	2 cut(s)	631, 906
Mox20I	TGGCCA	2 cut(s)	98, 1107
Mph1103I	ATGCAT	1 cut(s)	1291
MscI	TGGCCA	2 cut(s)	98, 1107
MseI	TTAA	5 cut(s)	423, 996, 1203, 1364, 1458
MslI	CAYNNNNRTG	2 cut(s)	587, 1220
Msp20I	TGGCCA	2 cut(s)	98, 1107
MspI	CCGG	2 cut(s)	55, 572
MspR9I	CCNGG	3 cut(s)	130, 182, 573
MunI	CAATTG	2 cut(s)	407, 1101
MvaI	CCWGG	2 cut(s)	130, 182
MwoI	GCNNNNNNNGC	1 cut(s)	37
NciI	CCSGG	1 cut(s)	573
NcoI	CCATGG	1 cut(s)	93
NdeI	CATATG	1 cut(s)	1291
NdeII	GATC	3 cut(s)	443, 762, 823
NheI	GCTAGC	1 cut(s)	1429
NlaIII	CATG	4 cut(s)	97, 586, 757, 971
NlaIV	GGNNCC	3 cut(s)	186, 324, 721
NmuCI	GTSAC	2 cut(s)	1152, 1159
NsiI	ATGCAT	1 cut(s)	1291
NspI	RCATGY	1 cut(s)	757
PaeR7I	CTCGAG	1 cut(s)	41
PagI	TCATGA	1 cut(s)	582
PasI	CCCWGGG	1 cut(s)	129
PfeI	GAWTC	4 cut(s)	287, 356, 668, 1320
PflMI	CCANNNNNTGG	2 cut(s)	94, 716
PkrI	GCNGC	2 cut(s)	171, 1405
PleI	GAGTC	4 cut(s)	260, 532, 538, 969
PpsI	GAGTC	4 cut(s)	260, 532, 538, 969
PpuMI	RGGWCCY	2 cut(s)	184, 323
PsiI	TTATAA	1 cut(s)	1416
Psp124BI	GAGCTC	1 cut(s)	370
Psp5II	RGGWCCY	2 cut(s)	184, 323
Psp6I	CCWGG	2 cut(s)	128, 180
PspFI	CCCAGC	2 cut(s)	641, 1278
PspGI	CCWGG	2 cut(s)	128, 180
PspN4I	GGNNCC	3 cut(s)	186, 324, 721
PspPI	GGNCC	4 cut(s)	184, 323, 719, 1467
PspPPI	RGGWCCY	2 cut(s)	184, 323
PstI	CTGCAG	1 cut(s)	1387
PsuI	RGATCY	1 cut(s)	762
RsaI	GTAC	2 cut(s)	149, 607
RsaNI	GTAC	2 cut(s)	148, 606
RseI	CAYNNNNRTG	2 cut(s)	587, 1220
SacI	GAGCTC	1 cut(s)	370
SaqAI	TTAA	5 cut(s)	423, 996, 1203, 1364, 1458
SatI	GCNGC	2 cut(s)	170, 1404
Sau3AI	GATC	3 cut(s)	443, 762, 823
Sau96I	GGNCC	4 cut(s)	184, 323, 719, 1467
ScaI	AGTACT	1 cut(s)	607
SchI	GAGTC	4 cut(s)	260, 532, 539, 970
ScrFI	CCNGG	3 cut(s)	130, 182, 573
SduI	GDGCHC	2 cut(s)	370, 1373
SfaNI	GCATC	6 cut(s)	223, 314, 793, 1063, 1276, 1416
SfcI	CTRYAG	2 cut(s)	291, 1383
Sfr274I	CTCGAG	1 cut(s)	41
SinI	GGWCC	4 cut(s)	184, 323, 719, 1467
SlaI	CTCGAG	1 cut(s)	41
SmiMI	CAYNNNNRTG	2 cut(s)	587, 1220
SmlI	CTYRAG	1 cut(s)	41
SmoI	CTYRAG	1 cut(s)	41
SpeI	ACTAGT	4 cut(s)	608, 770, 1025, 1156
SsiI	CCGC	3 cut(s)	351, 965, 1245
SspMI	CTAG	8 cut(s)	245, 284, 609, 771, 827, 1026, 1157, 1430
SstI	GAGCTC	1 cut(s)	370
StyD4I	CCNGG	3 cut(s)	128, 180, 571
StyI	CCWWGG	2 cut(s)	93, 1470
TaaI	ACNGT	2 cut(s)	193, 870
TaqI	TCGA	1 cut(s)	42
TaqII	GACCGA	1 cut(s)	851
TatI	WGTACW	1 cut(s)	605
TfiI	GAWTC	4 cut(s)	287, 356, 668, 1320
Tru1I	TTAA	5 cut(s)	423, 996, 1203, 1364, 1458
Tru9I	TTAA	5 cut(s)	423, 996, 1203, 1364, 1458
TscAI	CASTG	2 cut(s)	962, 1397
TseFI	GTSAC	2 cut(s)	1152, 1159
TseI	GCWGC	2 cut(s)	169, 1403
Tsp45I	GTSAC	2 cut(s)	1152, 1159
TspDTI	ATGAA	2 cut(s)	599, 1288
TspRI	CASTG	2 cut(s)	962, 1397
Van91I	CCANNNNNTGG	2 cut(s)	94, 716
VpaK11BI	GGWCC	4 cut(s)	184, 323, 719, 1467
XapI	RAATTY	2 cut(s)	431, 1082
XceI	RCATGY	1 cut(s)	757
XhoI	CTCGAG	1 cut(s)	41
XmiI	GTMKAC	1 cut(s)	294
XspI	CTAG	8 cut(s)	245, 284, 609, 771, 827, 1026, 1157, 1430
ZrmI	AGTACT	1 cut(s)	607
Zsp2I	ATGCAT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.