Rw2G001460

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005412
Physical Location & Seq
Forward (+)
1232942 .. 1235924
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001460.1

Sequence Viewer

Length: 729 bp
ATGTCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAGTGGTGAGACTATATATTCTCTCGCTCAACAGTCAAACAATGCAAAGTGTTTATACTTTGCTGAGGGTCGTGGAGGTATAAGTATGTGGGATGAGAGAACAGGAAACATCTCAGCCCAATGGAGTCCGCATGATGATAGAATCAATTCCATAGACTTTAACTCAGAAAATTCAAACATCATCACCACTAGTTCAACAGATGGAACTGCCTGCATTTGGGATTTGAGATGTATTGATGCAGATAAATTCAAAAGTTTGAAGACAATTGGCCACAAAAGGGCTGTGCATTCTGCCTACTTCTCACCTTCTGGAAGGTCGCTAGTGACGACAAGTCTTGATGACACAGTTGGTATATCAAGTGGCGTTAATTTTGAGGACATATCAAGGATATCCCATGACAATAATACAGGCAGATGGATTCCTAAGTTCAAGGCAATATGGGGCTGGGATGATGCATATGTCTTCATCGGAAATAAGAATAGAGGAATCGATGTCATCTCCCACCTTCAAAAAAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAATTCCGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCTACAGGTGGAGGCCAAGTTTATGTATGGACATTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

242

Amino Acids

26.81

Weight (kDa)

6.58

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 3 - 104 5e-08 WDR55
WD40_WDHD1_1st PF24817 3 - 240 3.7e-08 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 3 - 143 8.7e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 6 - 137 7.8e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 10 - 163 6.1e-12 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 21 - 159 5.2e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 31 - 144 6.5e-12 WDR3 first beta-propeller domain
EIF3I PF24805 31 - 131 2.8e-08 EIF3I
Beta-prop_EIPR1 PF23609 46 - 144 8.2e-09 EIPR1 beta-propeller
Beta-prop_WDR3_2nd PF25172 54 - 144 6.5e-06 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 55 - 239 2.4e-15 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 65 - 138 5.9e-07 RIG second beta-propeller
Beta-prop_THOC3 PF25174 72 - 202 3.3e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AciI CCGC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 2 cut(s) 253, 329
AfiI CCNNNNNNNGG 3 cut(s) 46, 300, 361
AgsI TTSAA 7 cut(s) 84, 258, 279, 334, 343, 514, 593
AhdI GACNNNNNGTC 1 cut(s) 414
AhlI ACTAGT 2 cut(s) 17, 272
AluBI AGCT 1 cut(s) 689
AluI AGCT 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 86
AoxI GGCC 2 cut(s) 352, 700
ApeKI GCWGC 1 cut(s) 653
ApoI RAATTY 2 cut(s) 253, 329
Asp700I GAANNNNTTC 1 cut(s) 229
AsuHPI GGTGA 4 cut(s) 101, 259, 378, 650
AsuNHI GCTAGC 1 cut(s) 679
BalI TGGCCA 1 cut(s) 354
BanII GRGCYC 1 cut(s) 623
BbsI GAAGAC 2 cut(s) 350, 538
BbvCI CCTCAGC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 640
BccI CCATC 4 cut(s) 23, 40, 278, 492
BcoDI GTCTC 1 cut(s) 86
BcuI ACTAGT 2 cut(s) 17, 272
BfaI CTAG 5 cut(s) 18, 74, 273, 404, 680
BfmI CTRYAG 1 cut(s) 690
BglII AGATCT 1 cut(s) 9
BisI GCNGC 1 cut(s) 654
BlsI GCNGC 1 cut(s) 655
BmeRI GACNNNNNGTC 1 cut(s) 414
BmsI GCATC 4 cut(s) 40, 310, 526, 666
BmtI GCTAGC 1 cut(s) 683
BpiI GAAGAC 2 cut(s) 350, 538
Bpu10I CCTNAGC 1 cut(s) 147
Bsa29I ATCGAT 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 300, 361
BseCI ATCGAT 1 cut(s) 573
BseGI GGATG 4 cut(s) 55, 181, 538, 681
BseLI CCNNNNNNNGG 3 cut(s) 46, 300, 361
BseMII CTCAG 4 cut(s) 45, 138, 210, 261
BseXI GCAGC 1 cut(s) 640
BseYI CCCAGC 1 cut(s) 528
BshFI GGCC 2 cut(s) 354, 702
BshVI ATCGAT 1 cut(s) 573
BslI CCNNNNNNNGG 3 cut(s) 46, 300, 361
BsmAI GTCTC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 2 cut(s) 354, 702
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 2 cut(s) 9, 70
BspACI CCGC 1 cut(s) 212
BspANI GGCC 2 cut(s) 354, 702
BspCNI CTCAG 4 cut(s) 46, 139, 209, 260
BspDI ATCGAT 1 cut(s) 573
BspOI GCTAGC 1 cut(s) 683
BssMI GATC 2 cut(s) 9, 70
Bst4CI ACNGT 2 cut(s) 117, 430
BstC8I GCNNGC 2 cut(s) 295, 681
BstDEI CTNAG 5 cut(s) 54, 147, 196, 247, 507
BstF5I GGATG 4 cut(s) 55, 181, 538, 681
BstKTI GATC 2 cut(s) 12, 73
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 9, 70
BstSFI CTRYAG 1 cut(s) 690
BstV1I GCAGC 1 cut(s) 640
BstV2I GAAGAC 2 cut(s) 350, 538
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 2 cut(s) 354, 702
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 4 cut(s) 55, 181, 538, 681
Cac8I GCNNGC 2 cut(s) 295, 681
ClaI ATCGAT 1 cut(s) 573
CviAII CATG 2 cut(s) 215, 479
CviJI RGCY 8 cut(s) 200, 354, 365, 528, 621, 653, 689, 702
CviKI_1 RGCY 8 cut(s) 200, 354, 365, 528, 621, 653, 689, 702
DdeI CTNAG 5 cut(s) 54, 147, 196, 247, 507
DpnI GATC 2 cut(s) 11, 72
DpnII GATC 2 cut(s) 9, 70
DraI TTTAAA 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 352
Eam1105I GACNNNNNGTC 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 623
Eco32I GATATC 1 cut(s) 474
EcoRV GATATC 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 2 cut(s) 218, 482
FatI CATG 2 cut(s) 214, 478
FauNDI CATATG 1 cut(s) 541
Fnu4HI GCNGC 1 cut(s) 654
FokI GGATG 4 cut(s) 62, 188, 545, 688
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 1 cut(s) 654
FspBI CTAG 5 cut(s) 18, 74, 273, 404, 680
GluI GCNGC 1 cut(s) 654
GsaI CCCAGC 1 cut(s) 532
HaeIII GGCC 2 cut(s) 354, 702
Hin1II CATG 2 cut(s) 218, 482
HinfI GANTC 4 cut(s) 208, 225, 502, 570
HphI GGTGA 4 cut(s) 101, 259, 378, 650
Hpy188I TCNGA 3 cut(s) 41, 250, 554
Hpy188III TCNNGA 2 cut(s) 393, 419
HpyAV CCTTC 3 cut(s) 390, 399, 599
HpyCH4III ACNGT 2 cut(s) 117, 430
HpyCH4V TGCA 6 cut(s) 128, 297, 323, 370, 539, 635
HpyF3I CTNAG 5 cut(s) 54, 147, 196, 247, 507
Hsp92II CATG 2 cut(s) 218, 482
Kzo9I GATC 2 cut(s) 9, 70
LmnI GCTCC 1 cut(s) 686
LpnPI CCDG 7 cut(s) 171, 307, 378, 477, 514, 669, 678
Lsp1109I GCAGC 1 cut(s) 640
LweI GCATC 4 cut(s) 40, 310, 526, 666
MaeI CTAG 5 cut(s) 18, 74, 273, 404, 680
MaeIII GTNAC 1 cut(s) 406
MalI GATC 2 cut(s) 11, 72
MboI GATC 2 cut(s) 9, 70
MboII GAAGA 2 cut(s) 355, 538
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 623
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 6 cut(s) 229, 253, 329, 348, 451, 636
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 217
MnlI CCTC 6 cut(s) 55, 142, 152, 451, 560, 692
Mox20I TGGCCA 1 cut(s) 354
Mph1103I ATGCAT 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 229
MscI TGGCCA 1 cut(s) 354
MseI TTAA 3 cut(s) 243, 450, 614
Msp20I TGGCCA 1 cut(s) 354
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 370
NdeI CATATG 1 cut(s) 541
NdeII GATC 2 cut(s) 9, 70
NheI GCTAGC 1 cut(s) 679
NlaIII CATG 2 cut(s) 218, 482
NmuCI GTSAC 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 229
PfeI GAWTC 3 cut(s) 225, 502, 570
PkrI GCNGC 1 cut(s) 655
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PsiI TTATAA 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 528
PsuI RGATCY 1 cut(s) 9
SaqAI TTAA 3 cut(s) 243, 450, 614
SatI GCNGC 1 cut(s) 654
Sau3AI GATC 2 cut(s) 9, 70
SchI GAGTC 1 cut(s) 217
SduI GDGCHC 1 cut(s) 623
SetI ASST 7 cut(s) 66, 163, 391, 401, 591, 691, 697
SfaNI GCATC 4 cut(s) 40, 310, 526, 666
SfcI CTRYAG 1 cut(s) 690
SpeI ACTAGT 2 cut(s) 17, 272
Sse9I AATT 6 cut(s) 229, 253, 329, 348, 451, 636
SsiI CCGC 1 cut(s) 212
SspMI CTAG 5 cut(s) 18, 74, 273, 404, 680
TaaI ACNGT 2 cut(s) 117, 430
TaqI TCGA 1 cut(s) 573
TasI AATT 6 cut(s) 229, 253, 329, 348, 451, 636
TfiI GAWTC 3 cut(s) 225, 502, 570
Tru1I TTAA 3 cut(s) 243, 450, 614
Tru9I TTAA 3 cut(s) 243, 450, 614
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 1 cut(s) 538
TspGWI ACGGA 1 cut(s) 630
XapI RAATTY 2 cut(s) 253, 329
XmnI GAANNNNTTC 1 cut(s) 229
XspI CTAG 5 cut(s) 18, 74, 273, 404, 680
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.