RLG00000015754

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005412
Physical Location & Seq
Forward (+)
1204177 .. 1206883
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015754

Sequence Viewer

Length: 1395 bp
ATGGCGACTCAGAAACTCACAGACTATGAGCGCAAGAGGCTCGAGAACATTCGCCGAAACGAGGAATTGATGGCTTCCCTAAAGCTCCAATCCATGGCCACCCAACTCTCTGCCTCAACCAAACGCCCCAGGGTTGAGACCAAATCGTACAAGGTCAATTCGGAGAAGCAGCCCAAGACCCAGGACCCAACTGTTATCAGGCGTTCTCTACGCACCAGAGGATTACCACCCGATGCGAAAGGACTAGGTGGTGATGGTGGTGATGACTCTGTGGTGAAGACCCTAGAATCTGTAGACCCTAAGGCATCGGCTCGCAAATTGGGTCCTCTTAGTATGAGAGATGTGTATAGCGGAGTATCGGATAGAGCTCTGATTGAGGCCATTTTGGGTGTTGAGAAGAAACCCCAATTGGGTTTGGAAGTTAAAGGTGAATTTGGGGGTTGCAAAGAGGAGAGATCAAGTGGTGCATTTATCTTAGTTGGTGGGGTTAGTTGTGATTTGAGTAAGAAATTAGAAGGAGTTGAGAGTGATTTGAAGGTGGAGTCTTTGACTCTGAATGAGGAGAATGTAGCCCGAGTTGTGCCGGGGAATGTCATGAATGTGAGATTTTTTCCGAGTACTAGTTTGAGGATGGTTGTGGTTGGGAATAAGGCTGGGAATGTTGGCTTTTGGAATGTTGATTCTAAGGAAGGGGAGGAAGGTGGGATATGTATGTATCACCCACATTCTGGTCCCATTTCGGGGATTTCTGTTCACCAAGATTGCATGTCAAAGATCTTTACTAGTTGCTATGATGGATTTATCCGATTGATGGATGCTGAGAAAGAGGTGTTTGATCTAGTATATTCAAGTGGTGAGACTATATATTCTCTCGCTCAACAGTCAAACAATGCAAAGTGTTTATACTTTGCTGAGGGTCGTGGAGGTATAAGTATGTGGGACGAGAGAACAGGAAACATCTCAGCCCAATGGAGTCCGCATGATGATAGAATCAATTCCATAGACTTTAACTCAGAAAATTCAAACATCATCACCACTAGTTCAACAGATGGAACTGCCTGCATTTGGGATTTGAGATGTATTGATGCAGATAAATTCAAAAGTTTGAAGACAATTGGCCACAAAAGGGCTGTGCATTCTGCCTACTTCTCACCTTCTGGAAGGTCGCTAGTGACGACAAGGGCAATATGGGGCTGGGATGATGCATATGTCTTCATCGGAAATAAGAATAGAGGAATCGATGTTATCTCCCACCTTCAAAAAAGAGCAGTTTTCACTTTAAAGAGCCCATATATGTCTGCAATTCCGTGTAGATTGGCTGCTCACCCTTATAATGTTGGGATGCTAGCAGGAGCTACAGGTGGAGGCCAAGTTTATGTATGGGCATTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

465

Amino Acids

50.83

Weight (kDa)

8.57

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 182 - 392 1.7e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 197 - 393 9.4e-16 Prp19 WD40 domain
WDR55 PF24796 197 - 359 3.5e-12 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 210 - 394 4.5e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 219 - 392 1.7e-11 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 256 - 393 6.9e-07 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 257 - 462 3.6e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 284 - 392 7.6e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 287 - 393 7e-09 WDR3 first beta-propeller domain
EIF3I PF24805 291 - 386 2.2e-07 EIF3I
Beta-prop_EIPR1 PF23609 303 - 391 1.9e-07 EIPR1 beta-propeller
Beta-prop_WDR5 PF25175 310 - 393 1.5e-10 WDR5 beta-propeller domain
Beta-prop_RIG_2nd PF23775 320 - 392 2.4e-06 RIG second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1332
AccB7I CCANNNNNTGG 2 cut(s) 94, 728
AccI GTMKAC 1 cut(s) 294
AciI CCGC 2 cut(s) 351, 977
AcoI YGGCCR 2 cut(s) 96, 1117
AcsI RAATTY 3 cut(s) 431, 1018, 1094
AfaI GTAC 2 cut(s) 149, 619
AfiI CCNNNNNNNGG 9 cut(s) 61, 94, 410, 728, 740, 741, 811, 1065, 1126
AgsI TTSAA 7 cut(s) 535, 849, 1023, 1044, 1099, 1108, 1259
AhlI ACTAGT 3 cut(s) 620, 782, 1037
AjnI CCWGG 2 cut(s) 128, 180
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 3 cut(s) 85, 368, 1355
AluI AGCT 3 cut(s) 85, 368, 1355
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 2 cut(s) 131, 851
Ama87I CYCGRG 2 cut(s) 41, 573
AoxI GGCC 4 cut(s) 96, 378, 1117, 1366
ApeKI GCWGC 2 cut(s) 169, 1319
ApoI RAATTY 3 cut(s) 431, 1018, 1094
Asp700I GAANNNNTTC 1 cut(s) 994
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 184, 323, 731
AsuC2I CCSGG 1 cut(s) 585
AsuNHI GCTAGC 1 cut(s) 1345
AvaI CYCGRG 2 cut(s) 41, 573
AvaII GGWCC 3 cut(s) 184, 323, 731
AxyI CCTNAGG 1 cut(s) 300
BalI TGGCCA 2 cut(s) 98, 1119
BanII GRGCYC 2 cut(s) 370, 1289
BbsI GAAGAC 3 cut(s) 284, 1115, 1204
Bbv12I GWGCWC 1 cut(s) 370
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 2 cut(s) 181, 1306
BccI CCATC 6 cut(s) 64, 248, 625, 788, 805, 1043
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 2 cut(s) 130, 182
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 2 cut(s) 131, 851
BcuI ACTAGT 3 cut(s) 620, 782, 1037
BfaI CTAG 8 cut(s) 245, 284, 621, 783, 839, 1038, 1169, 1346
BfmI CTRYAG 2 cut(s) 291, 1356
BglII AGATCT 1 cut(s) 774
BisI GCNGC 2 cut(s) 170, 1320
BlsI GCNGC 2 cut(s) 171, 1321
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 3 cut(s) 130, 182, 585
Bme18I GGWCC 3 cut(s) 184, 323, 731
BmeT110I CYCGRG 2 cut(s) 41, 573
BmgT120I GGNCC 3 cut(s) 184, 323, 731
BmiI GGNNCC 3 cut(s) 186, 324, 733
BmrFI CCNGG 3 cut(s) 130, 182, 585
BmsI GCATC 6 cut(s) 223, 314, 805, 1075, 1192, 1332
BmtI GCTAGC 1 cut(s) 1349
BpiI GAAGAC 3 cut(s) 284, 1115, 1204
Bpu10I CCTNAGC 1 cut(s) 912
BpuMI CCSGG 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 1239
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 5 cut(s) 93, 128, 129, 180, 584
BsaXI ACNNNNNCTCC 2 cut(s) 1356, 1386
Bsc4I CCNNNNNNNGG 9 cut(s) 61, 94, 410, 728, 740, 741, 811, 1065, 1126
Bse21I CCTNAGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 130, 182
BseCI ATCGAT 1 cut(s) 1239
BseDI CCNNGG 5 cut(s) 93, 128, 129, 180, 584
BseGI GGATG 4 cut(s) 636, 820, 1204, 1347
BseLI CCNNNNNNNGG 9 cut(s) 61, 94, 410, 728, 740, 741, 811, 1065, 1126
BseMII CTCAG 5 cut(s) 23, 810, 903, 975, 1026
BseRI GAGGAG 2 cut(s) 464, 575
BseXI GCAGC 2 cut(s) 181, 1306
BseYI CCCAGC 2 cut(s) 653, 1194
BshFI GGCC 4 cut(s) 98, 380, 1119, 1368
BshVI ATCGAT 1 cut(s) 1239
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 2 cut(s) 41, 573
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 2 cut(s) 717, 953
BslI CCNNNNNNNGG 9 cut(s) 61, 94, 410, 728, 740, 741, 811, 1065, 1126
BsmAI GTCTC 2 cut(s) 131, 851
BsmFI GGGAC 2 cut(s) 717, 953
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 4 cut(s) 98, 380, 1119, 1368
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 2 cut(s) 41, 573
Bsp1286I GDGCHC 2 cut(s) 370, 1289
Bsp143I GATC 3 cut(s) 455, 774, 835
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 351, 977
BspANI GGCC 4 cut(s) 98, 380, 1119, 1368
BspCNI CTCAG 5 cut(s) 22, 811, 904, 974, 1025
BspDI ATCGAT 1 cut(s) 1239
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 3 cut(s) 186, 324, 733
BspOI GCTAGC 1 cut(s) 1349
BspTNI GGTCTC 1 cut(s) 131
BssECI CCNNGG 5 cut(s) 93, 128, 129, 180, 584
BssMI GATC 3 cut(s) 455, 774, 835
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 2 cut(s) 130, 182
Bst4CI ACNGT 2 cut(s) 193, 882
BstC8I GCNNGC 3 cut(s) 313, 1060, 1347
BstDEI CTNAG 9 cut(s) 9, 300, 329, 475, 684, 819, 912, 961, 1012
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 636, 820, 1204, 1347
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 3 cut(s) 458, 777, 838
BstMAI GTCTC 2 cut(s) 131, 851
BstMBI GATC 3 cut(s) 455, 774, 835
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 2 cut(s) 130, 182
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 3 cut(s) 128, 180, 583
BstSFI CTRYAG 2 cut(s) 291, 1356
BstV1I GCAGC 2 cut(s) 181, 1306
BstV2I GAAGAC 3 cut(s) 284, 1115, 1204
BstX2I RGATCY 1 cut(s) 774
BstYI RGATCY 1 cut(s) 774
Bsu15I ATCGAT 1 cut(s) 1239
Bsu36I CCTNAGG 1 cut(s) 300
BsuRI GGCC 4 cut(s) 98, 380, 1119, 1368
BsuTUI ATCGAT 1 cut(s) 1239
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 4 cut(s) 636, 820, 1204, 1347
Cac8I GCNNGC 3 cut(s) 313, 1060, 1347
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 184, 323, 731
ClaI ATCGAT 1 cut(s) 1239
Csp6I GTAC 2 cut(s) 148, 618
CviAII CATG 4 cut(s) 94, 595, 766, 980
CviQI GTAC 2 cut(s) 148, 618
DdeI CTNAG 9 cut(s) 9, 300, 329, 475, 684, 819, 912, 961, 1012
DpnI GATC 3 cut(s) 457, 776, 837
DpnII GATC 3 cut(s) 455, 774, 835
DraI TTTAAA 1 cut(s) 1281
EaeI YGGCCR 2 cut(s) 96, 1117
Ecl136II GAGCTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 370, 1289
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 3 cut(s) 184, 323, 731
Eco53kI GAGCTC 1 cut(s) 368
Eco81I CCTNAGG 1 cut(s) 300
Eco88I CYCGRG 2 cut(s) 41, 573
EcoICRI GAGCTC 1 cut(s) 368
EcoO109I RGGNCCY 2 cut(s) 184, 323
EcoRII CCWGG 2 cut(s) 128, 180
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 2 cut(s) 370, 1289
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 97, 598, 769, 983
FaqI GGGAC 2 cut(s) 717, 953
FatI CATG 4 cut(s) 93, 594, 765, 979
FauNDI CATATG 1 cut(s) 1207
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 170, 1320
FokI GGATG 4 cut(s) 643, 827, 1211, 1354
FriOI GRGCYC 2 cut(s) 370, 1289
Fsp4HI GCNGC 2 cut(s) 170, 1320
FspBI CTAG 8 cut(s) 245, 284, 621, 783, 839, 1038, 1169, 1346
GlaI GCGC 1 cut(s) 32
GluI GCNGC 2 cut(s) 170, 1320
GsaI CCCAGC 2 cut(s) 657, 1198
HaeIII GGCC 4 cut(s) 98, 380, 1119, 1368
HapII CCGG 1 cut(s) 584
HhaI GCGC 1 cut(s) 33
Hin1II CATG 4 cut(s) 97, 598, 769, 983
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HinfI GANTC 9 cut(s) 7, 266, 287, 542, 550, 680, 973, 990, 1236
HpaII CCGG 1 cut(s) 584
Hpy166II GTNNAC 2 cut(s) 295, 754
Hpy188I TCNGA 9 cut(s) 12, 163, 361, 372, 555, 615, 806, 1015, 1220
Hpy188III TCNNGA 3 cut(s) 43, 595, 1158
Hpy8I GTNNAC 2 cut(s) 295, 754
HpyAV CCTTC 7 cut(s) 509, 529, 683, 692, 1155, 1164, 1265
HpyCH4III ACNGT 2 cut(s) 193, 882
HpyCH4V TGCA 9 cut(s) 444, 467, 765, 893, 1062, 1088, 1135, 1205, 1301
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 9 cut(s) 9, 300, 329, 475, 684, 819, 912, 961, 1012
Hsp92II CATG 4 cut(s) 97, 598, 769, 983
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 3 cut(s) 455, 774, 835
LmnI GCTCC 2 cut(s) 90, 1352
Lsp1109I GCAGC 2 cut(s) 181, 1306
LweI GCATC 6 cut(s) 223, 314, 805, 1075, 1192, 1332
MaeI CTAG 8 cut(s) 245, 284, 621, 783, 839, 1038, 1169, 1346
MaeIII GTNAC 1 cut(s) 1171
MalI GATC 3 cut(s) 457, 776, 837
MboI GATC 3 cut(s) 455, 774, 835
MboII GAAGA 4 cut(s) 289, 409, 1120, 1204
MfeI CAATTG 2 cut(s) 407, 1113
MflI RGATCY 1 cut(s) 774
MhlI GDGCHC 2 cut(s) 370, 1289
MlsI TGGCCA 2 cut(s) 98, 1119
MluNI TGGCCA 2 cut(s) 98, 1119
MlyI GAGTC 4 cut(s) 260, 544, 551, 982
Mox20I TGGCCA 2 cut(s) 98, 1119
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 1 cut(s) 994
MscI TGGCCA 2 cut(s) 98, 1119
MseI TTAA 3 cut(s) 423, 1008, 1280
MslI CAYNNNNRTG 1 cut(s) 599
Msp20I TGGCCA 2 cut(s) 98, 1119
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 3 cut(s) 130, 182, 585
MunI CAATTG 2 cut(s) 407, 1113
Mva1269I GAATGC 1 cut(s) 1135
MvaI CCWGG 2 cut(s) 130, 182
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 585
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 1207
NdeII GATC 3 cut(s) 455, 774, 835
NheI GCTAGC 1 cut(s) 1345
NlaIII CATG 4 cut(s) 97, 598, 769, 983
NlaIV GGNNCC 3 cut(s) 186, 324, 733
NmuCI GTSAC 1 cut(s) 1171
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 1 cut(s) 769
PaeR7I CTCGAG 1 cut(s) 41
PagI TCATGA 1 cut(s) 594
PasI CCCWGGG 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 1172
PctI GAATGC 1 cut(s) 1135
PdmI GAANNNNTTC 1 cut(s) 994
PfeI GAWTC 4 cut(s) 287, 680, 990, 1236
PflMI CCANNNNNTGG 2 cut(s) 94, 728
PkrI GCNGC 2 cut(s) 171, 1321
PleI GAGTC 4 cut(s) 260, 544, 550, 981
PpsI GAGTC 4 cut(s) 260, 544, 550, 981
PpuMI RGGWCCY 2 cut(s) 184, 323
PsiI TTATAA 1 cut(s) 1332
Psp124BI GAGCTC 1 cut(s) 370
Psp5II RGGWCCY 2 cut(s) 184, 323
Psp6I CCWGG 2 cut(s) 128, 180
PspFI CCCAGC 2 cut(s) 653, 1194
PspGI CCWGG 2 cut(s) 128, 180
PspN4I GGNNCC 3 cut(s) 186, 324, 733
PspPI GGNCC 3 cut(s) 184, 323, 731
PspPPI RGGWCCY 2 cut(s) 184, 323
PsuI RGATCY 1 cut(s) 774
RsaI GTAC 2 cut(s) 149, 619
RsaNI GTAC 2 cut(s) 148, 618
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 370
SaqAI TTAA 3 cut(s) 423, 1008, 1280
SatI GCNGC 2 cut(s) 170, 1320
Sau3AI GATC 3 cut(s) 455, 774, 835
Sau96I GGNCC 3 cut(s) 184, 323, 731
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 4 cut(s) 260, 544, 551, 982
ScrFI CCNGG 3 cut(s) 130, 182, 585
SduI GDGCHC 2 cut(s) 370, 1289
SfaNI GCATC 6 cut(s) 223, 314, 805, 1075, 1192, 1332
SfcI CTRYAG 2 cut(s) 291, 1356
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 3 cut(s) 184, 323, 731
SlaI CTCGAG 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 3 cut(s) 620, 782, 1037
SsiI CCGC 2 cut(s) 351, 977
SspMI CTAG 8 cut(s) 245, 284, 621, 783, 839, 1038, 1169, 1346
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 3 cut(s) 128, 180, 583
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 193, 882
TaqI TCGA 2 cut(s) 42, 1239
TatI WGTACW 1 cut(s) 617
TfiI GAWTC 4 cut(s) 287, 680, 990, 1236
Tru1I TTAA 3 cut(s) 423, 1008, 1280
Tru9I TTAA 3 cut(s) 423, 1008, 1280
TseFI GTSAC 1 cut(s) 1171
TseI GCWGC 2 cut(s) 169, 1319
Tsp45I GTSAC 1 cut(s) 1171
TspDTI ATGAA 2 cut(s) 611, 1204
TspGWI ACGGA 1 cut(s) 1296
Van91I CCANNNNNTGG 2 cut(s) 94, 728
VpaK11BI GGWCC 3 cut(s) 184, 323, 731
XapI RAATTY 3 cut(s) 431, 1018, 1094
XceI RCATGY 1 cut(s) 769
XhoI CTCGAG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 994
XspI CTAG 8 cut(s) 245, 284, 621, 783, 839, 1038, 1169, 1346
ZrmI AGTACT 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.