Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0005013

Physical Location & Seq

Genomic Coordinates Reverse (-)

1149921 .. 1152272

Gene Structure 2352 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g01910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGGAGTTGGGCAGATTATCCCGTTTAGAATGGGCATCGCAGTGTTAAGTCTGTTGCTGCAATTGCCAGCTATCTTTGCATCCAAGTTTGTCATACAGTCAAAGGATTGTAGTGATCACATTGCCTATTCAAGCTCTCATGGTCATGAATTGTTTTACATAAACGGGAATTCAGTAGAGAAAACTATATTCTGTGCAGCTCTTCGGACATACTATGCAAATGGTTGCATTTTAGAAGGCAACCTTGGAAGTTACCATTGTCTATCAGACCTTTCACTAGTTGATTTTACCTTGAGGACAGGGAGGAAACTTCTGCAGAAGGAGTCAAGGGATAAATCCACTTTCGACAACAGAGAGGAAAAAAATGATTTTGCATATACGTCTGCGACGAAAAAGATTGGAATAGCAGCAAGTGGAATGTTTCTCACGTGTTGTGTTTTTCTATGTCCTTGCATTTTTAAGAAAAGGCGAGAAAGTGTGCACAGAGTTCTTGCAAGGGAGCCAAATTCAACGGATTCAGTTTTTTCTTTTGAAGTGAGTTCTTCAGATCCTGAAAAGATCCTGGCCAGTCCGCATCGTGTGCCACGTAGTCCACATCATGTGCCGCCTAGTCCTAGATTTTCAATGTCTCCAAAGCCCATTGGAATTGGATCAGTACATCTCAGTTTGAATCAGATTGTAAAAGTAACTCACAACTTCTCTCAATCGCAACAAATAGGTGAAGGAGGTTTTGGAACTGTATACAAGGCTCGGTTAGATGATGGCCAAGTGGTTTCTATCAAACGTGCAAAGAAGGAACACTTTGAGAATTTGCAAACTGAATTCAGCAGTGAAGTTGAACTGCTTGCCAAAATTGATCATCGGAATCTTGTAAAGCTACTAGGTTATGTTGATGAAGGAAACGAGCGTCTTATCATTACGGAGTATGTGCAGAACGGTACTCTTAGAGAACATCTGGATTGTCTGCATGGGAAAATCCTAGAGTTCAACCAGCGACTTGAAATTGCTATCGATGTTGCTCATGCCCTTACCTATCTTCACACATATTCTGAAAAACAAATCATCCATCGAGACGTGAAGTCCTCCAACATTCTTCTCACAGAAAGCATGAGAGCCAAAGTGGCCGATTTTGGGTTTGCAAGGCTTGGCCCGGTGGACACAGATCGATCACACATTTCAACCAAAGTGAAAGGAACTGTCGGTTATCTTGACCCTGAGTATATGAAAACCTATCAACTCACCCCGAAAAGTGATGTTTACTCGTTCGCGATCTTACTAATAGAAATTTTGACTGGCCGTCGTCCAGTGGAGTTGAAGCGACCTGTTGAAGAGCGGGTAACACTCAGATGGGCGTTCAAGAAGATGAATGAAGGCAGGGTGGCAGAATTGGTTGATCCATTGATGGAGGAAACTATAGATGCAGAGGTTCTGATGAAAACCTTCGATCTGGCGATCCAATGTGCAGCCCCCATCCGAGTTGATAGGCCGGACATGAAAGCGGTGGGAGAGCAGTTGTGGACAATAAGGGCAGACTACCTTAGAAGTGTCAAGAGAGGGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

521

Amino Acids

58.68

Weight (kDa)

8.63

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	234 - 440	5.3e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	235 - 505	7.8e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G11520
fragaria_vesca	FvH4_1g02470 FvH4_1g02470 FvH4_1g02470 FvH4_1g02470 FvH4_1g02470 FvH4_1g02470 FvH4_1g02470
malus_domestica	MD02G1022200.v1.1 MD15G1164700.v1.1
prunus_persica	Prupe.7G250000_v2.0.a1 Prupe.7G250000_v2.0.a1 Prupe.7G250000_v2.0.a1 Prupe.7G250000_v2.0.a1 Prupe.7G250000_v2.0.a1 Prupe.7G250000_v2.0.a1
pyrus_communis	pycom02g01910 pycom15g14770
rosa_chinensis	RchiOBHm_Chr2g0087441
rosa_laevigata	RLG00000015869
rosa_multiflora	Rmu_sc0006064.1_g000012
rosa_roxburghii	Rroxscaffold_2G00153530
rosa_rugosa	Rorug01G0475300 Rorug01G0475400 Rorug01G0475500
rosa_samantha	Rh2AG028800 Rh2BG029100 Rh2CG029100 Rh2DG028900
rosa_wichuraiana	Rw2G002300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1333
AccI	GTMKAC	1 cut(s)	741
AccII	CGCG	1 cut(s)	1268
AciI	CCGC	4 cut(s)	572, 605, 1333, 1499
AclWI	GGATC	5 cut(s)	542, 553, 658, 1388, 1447
AcoI	YGGCCR	4 cut(s)	564, 763, 1122, 1294
AcsI	RAATTY	5 cut(s)	169, 505, 808, 821, 1284
AcuI	CTGAAG	1 cut(s)	528
AcvI	CACGTG	1 cut(s)	429
AfaI	GTAC	2 cut(s)	657, 940
AfiI	CCNNNNNNNGG	1 cut(s)	1131
AflIII	ACRYGT	1 cut(s)	428
AhdI	GACNNNNNGTC	2 cut(s)	1078, 1296
AhlI	ACTAGT	1 cut(s)	277
AjiI	CACGTC	1 cut(s)	1075
AjnI	CCWGG	1 cut(s)	561
AjuI	GAANNNNNNNTTGG	4 cut(s)	228, 260, 714, 746
AluBI	AGCT	4 cut(s)	71, 135, 200, 877
AluI	AGCT	4 cut(s)	71, 135, 200, 877
Alw21I	GWGCWC	1 cut(s)	483
Alw26I	GTCTC	2 cut(s)	633, 1065
Alw44I	GTGCAC	1 cut(s)	479
AlwI	GGATC	5 cut(s)	542, 553, 658, 1388, 1447
AlwNI	CAGNNNCTG	1 cut(s)	551
AoxI	GGCC	6 cut(s)	564, 763, 1122, 1147, 1294, 1484
ApaLI	GTGCAC	1 cut(s)	479
ApeKI	GCWGC	4 cut(s)	58, 197, 407, 1463
ApoI	RAATTY	5 cut(s)	169, 505, 808, 821, 1284
Asp700I	GAANNNNTTC	1 cut(s)	1439
AspS9I	GGNCC	1 cut(s)	1148
AsuC2I	CCSGG	1 cut(s)	1151
AsuHPI	GGTGA	2 cut(s)	731, 1231
BaeGI	GKGCMC	1 cut(s)	483
BalI	TGGCCA	2 cut(s)	566, 765
BbrPI	CACGTG	1 cut(s)	429
Bbv12I	GWGCWC	1 cut(s)	483
BbvI	GCAGC	4 cut(s)	45, 209, 419, 1475
BccI	CCATC	5 cut(s)	755, 1074, 1341, 1396, 1478
BceAI	ACGGC	1 cut(s)	1281
BciT130I	CCWGG	1 cut(s)	563
BclI	TGATCA	2 cut(s)	115, 856
BcnI	CCSGG	1 cut(s)	1151
BcoDI	GTCTC	2 cut(s)	633, 1065
BcuI	ACTAGT	1 cut(s)	277
BfaI	CTAG	5 cut(s)	278, 609, 615, 881, 980
BfmI	CTRYAG	2 cut(s)	314, 1413
BglI	GCCNNNNNGGC	1 cut(s)	1121
BisI	GCNGC	5 cut(s)	59, 198, 408, 605, 1464
BlsI	GCNGC	5 cut(s)	60, 199, 409, 606, 1465
Bme1390I	CCNGG	2 cut(s)	563, 1151
BmeRI	GACNNNNNGTC	2 cut(s)	1078, 1296
BmgBI	CACGTC	1 cut(s)	1075
BmgT120I	GGNCC	1 cut(s)	1148
BmiI	GGNNCC	1 cut(s)	501
BmrFI	CCNGG	2 cut(s)	563, 1151
BmsI	GCATC	4 cut(s)	45, 89, 583, 1408
BpuEI	CTTGAG	1 cut(s)	313
BpuMI	CCSGG	1 cut(s)	1151
Bsa29I	ATCGAT	2 cut(s)	1011, 1165
BsaAI	YACGTR	2 cut(s)	429, 587
BsaJI	CCNNGG	1 cut(s)	244
Bsc4I	CCNNNNNNNGG	1 cut(s)	1131
Bse1I	ACTGG	3 cut(s)	567, 1297, 1304
Bse3DI	GCAATG	1 cut(s)	120
BseBI	CCWGG	1 cut(s)	563
BseCI	ATCGAT	2 cut(s)	1011, 1165
BseDI	CCNNGG	1 cut(s)	244
BseGI	GGATG	3 cut(s)	80, 1062, 1470
BseLI	CCNNNNNNNGG	1 cut(s)	1131
BseMI	GCAATG	1 cut(s)	120
BseMII	CTCAG	3 cut(s)	676, 1206, 1357
BseNI	ACTGG	3 cut(s)	567, 1297, 1304
BseSI	GKGCMC	1 cut(s)	483
BseXI	GCAGC	4 cut(s)	45, 209, 419, 1475
BsgI	GTGCAG	3 cut(s)	216, 950, 1482
Bsh1236I	CGCG	1 cut(s)	1268
BshFI	GGCC	6 cut(s)	566, 765, 1124, 1149, 1296, 1486
BshVI	ATCGAT	2 cut(s)	1011, 1165
BsiHKAI	GWGCWC	1 cut(s)	483
BsiSI	CCGG	2 cut(s)	1151, 1487
BslI	CCNNNNNNNGG	1 cut(s)	1131
BsmAI	GTCTC	2 cut(s)	633, 1065
BsmBI	CGTCTC	1 cut(s)	1065
BsnI	GGCC	6 cut(s)	566, 765, 1124, 1149, 1296, 1486
Bsp1286I	GDGCHC	1 cut(s)	483
Bsp68I	TCGCGA	1 cut(s)	1268
BspACI	CCGC	4 cut(s)	572, 605, 1333, 1499
BspANI	GGCC	6 cut(s)	566, 765, 1124, 1149, 1296, 1486
BspCNI	CTCAG	3 cut(s)	675, 1207, 1356
BspDI	ATCGAT	2 cut(s)	1011, 1165
BspFNI	CGCG	1 cut(s)	1268
BspHI	TCATGA	1 cut(s)	145
BspLI	GGNNCC	1 cut(s)	501
BspMAI	CTGCAG	1 cut(s)	318
BspPI	GGATC	5 cut(s)	542, 553, 658, 1388, 1447
BspQI	GCTCTTC	2 cut(s)	207, 1323
BsrBI	CCGCTC	1 cut(s)	1333
BsrDI	GCAATG	1 cut(s)	120
BsrI	ACTGG	3 cut(s)	567, 1297, 1304
BssECI	CCNNGG	1 cut(s)	244
BssNAI	GTATAC	1 cut(s)	742
BssT1I	CCWWGG	1 cut(s)	244
Bst1107I	GTATAC	1 cut(s)	742
Bst2UI	CCWGG	1 cut(s)	563
Bst4CI	ACNGT	4 cut(s)	99, 739, 938, 1198
Bst6I	CTCTTC	2 cut(s)	207, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	580
BstBAI	YACGTR	2 cut(s)	429, 587
BstC8I	GCNNGC	2 cut(s)	69, 846
BstDEI	CTNAG	5 cut(s)	662, 944, 1215, 1343, 1538
BstF5I	GGATG	3 cut(s)	80, 1062, 1470
BstFNI	CGCG	1 cut(s)	1268
BstMAI	GTCTC	2 cut(s)	633, 1065
BstMWI	GCNNNNNNNGC	4 cut(s)	64, 77, 580, 1121
BstNI	CCWGG	1 cut(s)	563
BstSCI	CCNGG	2 cut(s)	561, 1149
BstSFI	CTRYAG	2 cut(s)	314, 1413
BstSLI	GKGCMC	1 cut(s)	483
BstUI	CGCG	1 cut(s)	1268
BstV1I	GCAGC	4 cut(s)	45, 209, 419, 1475
BstX2I	RGATCY	2 cut(s)	547, 558
BstYI	RGATCY	2 cut(s)	547, 558
BstZ17I	GTATAC	1 cut(s)	742
Bsu15I	ATCGAT	2 cut(s)	1011, 1165
BsuRI	GGCC	6 cut(s)	566, 765, 1124, 1149, 1296, 1486
BsuTUI	ATCGAT	2 cut(s)	1011, 1165
BtgZI	GCGATG	1 cut(s)	22
BtrI	CACGTC	1 cut(s)	1075
BtsCI	GGATG	3 cut(s)	80, 1062, 1470
BtsI	GCAGTG	2 cut(s)	48, 835
BtsIMutI	CAGTG	3 cut(s)	48, 835, 1311
BtuMI	TCGCGA	1 cut(s)	1268
Cac8I	GCNNGC	2 cut(s)	69, 846
CaiI	CAGNNNCTG	1 cut(s)	551
CciI	TCATGA	1 cut(s)	145
Cfr13I	GGNCC	1 cut(s)	1148
ClaI	ATCGAT	2 cut(s)	1011, 1165
CseI	GACGC	1 cut(s)	896
Csp6I	GTAC	2 cut(s)	656, 939
CviAII	CATG	7 cut(s)	140, 146, 599, 968, 1022, 1108, 1492
CviQI	GTAC	2 cut(s)	656, 939
DdeI	CTNAG	5 cut(s)	662, 944, 1215, 1343, 1538
DriI	GACNNNNNGTC	2 cut(s)	1078, 1296
EaeI	YGGCCR	4 cut(s)	564, 763, 1122, 1294
Eam1104I	CTCTTC	2 cut(s)	207, 1323
Eam1105I	GACNNNNNGTC	2 cut(s)	1078, 1296
EarI	CTCTTC	2 cut(s)	207, 1323
Eco130I	CCWWGG	1 cut(s)	244
Eco57I	CTGAAG	1 cut(s)	528
Eco72I	CACGTG	1 cut(s)	429
EcoRI	GAATTC	2 cut(s)	169, 821
EcoRII	CCWGG	1 cut(s)	561
EcoT14I	CCWWGG	1 cut(s)	244
ErhI	CCWWGG	1 cut(s)	244
Esp3I	CGTCTC	1 cut(s)	1065
FaeI	CATG	7 cut(s)	143, 149, 602, 971, 1025, 1111, 1495
FalI	AAGNNNNNCTT	4 cut(s)	228, 260, 785, 817
FatI	CATG	7 cut(s)	139, 145, 598, 967, 1021, 1107, 1491
FauI	CCCGC	1 cut(s)	1326
FbaI	TGATCA	2 cut(s)	115, 856
FblI	GTMKAC	1 cut(s)	741
Fnu4HI	GCNGC	5 cut(s)	59, 198, 408, 605, 1464
FokI	GGATG	3 cut(s)	67, 1049, 1457
Fsp4HI	GCNGC	5 cut(s)	59, 198, 408, 605, 1464
FspBI	CTAG	5 cut(s)	278, 609, 615, 881, 980
GluI	GCNGC	5 cut(s)	59, 198, 408, 605, 1464
HaeIII	GGCC	6 cut(s)	566, 765, 1124, 1149, 1296, 1486
HapII	CCGG	2 cut(s)	1151, 1487
HgaI	GACGC	1 cut(s)	896
Hin1II	CATG	7 cut(s)	143, 149, 602, 971, 1025, 1111, 1495
HinfI	GANTC	4 cut(s)	323, 515, 670, 865
HpaII	CCGG	2 cut(s)	1151, 1487
HphI	GGTGA	2 cut(s)	731, 1231
Hpy166II	GTNNAC	6 cut(s)	481, 593, 742, 1156, 1258, 1518
Hpy188I	TCNGA	9 cut(s)	207, 268, 547, 675, 864, 1051, 1346, 1431, 1475
Hpy188III	TCNNGA	8 cut(s)	146, 551, 956, 1070, 1208, 1267, 1357, 1549
Hpy8I	GTNNAC	6 cut(s)	481, 593, 742, 1156, 1258, 1518
Hpy99I	CGWCG	2 cut(s)	391, 1302
HpyAV	CCTTC	7 cut(s)	230, 313, 716, 787, 890, 1364, 1450
HpyCH4III	ACNGT	4 cut(s)	99, 739, 938, 1198
HpyCH4IV	ACGT	5 cut(s)	380, 428, 586, 784, 1074
HpyF10VI	GCNNNNNNNGC	4 cut(s)	64, 77, 580, 1121
HpyF3I	CTNAG	5 cut(s)	662, 944, 1215, 1343, 1538
HpySE526I	ACGT	5 cut(s)	380, 428, 586, 784, 1074
Hsp92II	CATG	7 cut(s)	143, 149, 602, 971, 1025, 1111, 1495
Ksp22I	TGATCA	2 cut(s)	115, 856
LguI	GCTCTTC	2 cut(s)	207, 1323
LmnI	GCTCC	1 cut(s)	499
Lsp1109I	GCAGC	4 cut(s)	45, 209, 419, 1475
LweI	GCATC	4 cut(s)	45, 89, 583, 1408
MaeI	CTAG	5 cut(s)	278, 609, 615, 881, 980
MaeII	ACGT	5 cut(s)	380, 428, 586, 784, 1074
MaeIII	GTNAC	3 cut(s)	251, 685, 1336
MbiI	CCGCTC	1 cut(s)	1333
MboII	GAAGA	6 cut(s)	194, 534, 1028, 1085, 1340, 1372
MfeI	CAATTG	1 cut(s)	62
MflI	RGATCY	2 cut(s)	547, 558
MhlI	GDGCHC	1 cut(s)	483
MlsI	TGGCCA	2 cut(s)	566, 765
MluNI	TGGCCA	2 cut(s)	566, 765
MlyI	GAGTC	1 cut(s)	332
MmeI	TCCRAC	1 cut(s)	1110
MnlI	CCTC	8 cut(s)	288, 297, 349, 719, 1093, 1399, 1417, 1547
Mox20I	TGGCCA	2 cut(s)	566, 765
MroXI	GAANNNNTTC	1 cut(s)	1439
MscI	TGGCCA	2 cut(s)	566, 765
MseI	TTAA	2 cut(s)	47, 459
MslI	CAYNNNNRTG	3 cut(s)	41, 144, 1345
Msp20I	TGGCCA	2 cut(s)	566, 765
MspI	CCGG	2 cut(s)	1151, 1487
MspR9I	CCNGG	2 cut(s)	563, 1151
MunI	CAATTG	1 cut(s)	62
MvaI	CCWGG	1 cut(s)	563
MvnI	CGCG	1 cut(s)	1268
MwoI	GCNNNNNNNGC	4 cut(s)	64, 77, 580, 1121
NciI	CCSGG	1 cut(s)	1151
NlaIII	CATG	7 cut(s)	143, 149, 602, 971, 1025, 1111, 1495
NlaIV	GGNNCC	1 cut(s)	501
NruI	TCGCGA	1 cut(s)	1268
PagI	TCATGA	1 cut(s)	145
PciSI	GCTCTTC	2 cut(s)	207, 1323
PcsI	WCGNNNNNNNCGW	2 cut(s)	386, 583
PdmI	GAANNNNTTC	1 cut(s)	1439
PfeI	GAWTC	3 cut(s)	515, 670, 865
PkrI	GCNGC	5 cut(s)	60, 199, 409, 606, 1465
PleI	GAGTC	1 cut(s)	331
PmaCI	CACGTG	1 cut(s)	429
PmlI	CACGTG	1 cut(s)	429
PpsI	GAGTC	1 cut(s)	331
Ppu21I	YACGTR	2 cut(s)	429, 587
Psp6I	CCWGG	1 cut(s)	561
PspCI	CACGTG	1 cut(s)	429
PspGI	CCWGG	1 cut(s)	561
PspN4I	GGNNCC	1 cut(s)	501
PspPI	GGNCC	1 cut(s)	1148
PstI	CTGCAG	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	551
PsuI	RGATCY	2 cut(s)	547, 558
RruI	TCGCGA	1 cut(s)	1268
RsaI	GTAC	2 cut(s)	657, 940
RsaNI	GTAC	2 cut(s)	656, 939
RseI	CAYNNNNRTG	3 cut(s)	41, 144, 1345
SapI	GCTCTTC	2 cut(s)	207, 1323
SaqAI	TTAA	2 cut(s)	47, 459
SatI	GCNGC	5 cut(s)	59, 198, 408, 605, 1464
Sau96I	GGNCC	1 cut(s)	1148
SchI	GAGTC	1 cut(s)	332
ScrFI	CCNGG	2 cut(s)	563, 1151
SduI	GDGCHC	1 cut(s)	483
SfaNI	GCATC	4 cut(s)	45, 89, 583, 1408
SfcI	CTRYAG	2 cut(s)	314, 1413
SmiMI	CAYNNNNRTG	3 cut(s)	41, 144, 1345
SmlI	CTYRAG	1 cut(s)	292
SmoI	CTYRAG	1 cut(s)	292
SpeI	ACTAGT	1 cut(s)	277
SsiI	CCGC	4 cut(s)	572, 605, 1333, 1499
SspMI	CTAG	5 cut(s)	278, 609, 615, 881, 980
StyD4I	CCNGG	2 cut(s)	561, 1149
StyI	CCWWGG	1 cut(s)	244
TaaI	ACNGT	4 cut(s)	99, 739, 938, 1198
TaiI	ACGT	5 cut(s)	383, 431, 589, 787, 1077
TaqI	TCGA	5 cut(s)	345, 1011, 1069, 1165, 1443
TatI	WGTACW	1 cut(s)	655
TauI	GCSGC	1 cut(s)	607
TfiI	GAWTC	3 cut(s)	515, 670, 865
Tru1I	TTAA	2 cut(s)	47, 459
Tru9I	TTAA	2 cut(s)	47, 459
TscAI	CASTG	3 cut(s)	48, 835, 1311
TseI	GCWGC	4 cut(s)	58, 197, 407, 1463
TspDTI	ATGAA	7 cut(s)	162, 909, 1238, 1379, 1383, 1448, 1508
TspGWI	ACGGA	2 cut(s)	527, 935
TspRI	CASTG	3 cut(s)	48, 835, 1311
VneI	GTGCAC	1 cut(s)	479
XapI	RAATTY	5 cut(s)	169, 505, 808, 821, 1284
XmiI	GTMKAC	1 cut(s)	741
XmnI	GAANNNNTTC	1 cut(s)	1439
XspI	CTAG	5 cut(s)	278, 609, 615, 881, 980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.