pycom02g03680

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013733
Physical Location & Seq
Reverse (-)
2471743 .. 2472279
537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGACAGCCCACGAAGCCTCCTCTTCATCCTCCTTCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTTAGCGGCGAAGACACGCGCAAGGGCTTCACGGGCCACCTCCACGATGCAATCGAAGACAGGGGATACAAAGCTTATATGGATGAGGACGATCTAAAAAGAGGGGAAGAAATAAAAGAGGAACTCTACCGGGGAATCGAAGAGTCGAGGATCTCTATCATTGTCTTCTCAAAGAAGTACGCAGATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGAGTGCAGATCCAAAATGGGGCGATATGTTTTGCCAATATTCTATCATGTTGATCCTTCACATGTCAGGAAGCAGAACGGAGATTTAGCCAAAACATTTCAGAATTACGAAGAGGACATCCGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGGGTAAATCAGTGGAGAGAGGCTCTTACAGAGGCTGCAAATTTGGCCGGCCACCATATTCAACTCACTGACAATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

179

Amino Acids

20.9

Weight (kDa)

5.69

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 175 6.2e-48 TIR domain
TIR_2 PF13676 21 - 136 1.4e-20 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AciI CCGC 1 cut(s) 78
AclWI GGATC 3 cut(s) 230, 297, 341
AcoI YGGCCR 2 cut(s) 501, 505
AcsI RAATTY 1 cut(s) 496
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 312
AflIII ACRYGT 2 cut(s) 60, 355
AgsI TTSAA 2 cut(s) 37, 518
AjiI CACGTC 1 cut(s) 61
AluBI AGCT 4 cut(s) 72, 146, 280, 446
AluI AGCT 4 cut(s) 72, 146, 280, 446
AlwI GGATC 3 cut(s) 230, 297, 341
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 3 cut(s) 106, 501, 505
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 496
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 295
BbsI GAAGAC 3 cut(s) 90, 135, 229
BbvI GCAGC 1 cut(s) 478
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 2 cut(s) 79, 492
BlsI GCNGC 2 cut(s) 80, 493
Bme1390I CCNGG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 3 cut(s) 90, 135, 229
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 202
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse118I RCCGGY 1 cut(s) 503
Bse8I GATNNNNATC 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 3 cut(s) 26, 160, 411
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 10
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 3 cut(s) 108, 503, 507
BsiSI CCGG 2 cut(s) 202, 504
BslI CCNNNNNNNGG 1 cut(s) 312
BsnI GGCC 3 cut(s) 108, 503, 507
Bsp143I GATC 5 cut(s) 163, 222, 288, 302, 346
BspACI CCGC 1 cut(s) 78
BspANI GGCC 3 cut(s) 108, 503, 507
BspFNI CGCG 1 cut(s) 91
BspPI GGATC 3 cut(s) 230, 297, 341
BsrFI RCCGGY 1 cut(s) 503
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 5 cut(s) 163, 222, 288, 302, 346
Bst6I CTCTTC 3 cut(s) 28, 207, 399
BstC8I GCNNGC 1 cut(s) 505
BstF5I GGATG 3 cut(s) 26, 160, 411
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 5 cut(s) 166, 225, 291, 305, 349
BstMBI GATC 5 cut(s) 163, 222, 288, 302, 346
BstMWI GCNNNNNNNGC 4 cut(s) 14, 78, 105, 500
BstNSI RCATGY 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 201
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 3 cut(s) 90, 135, 229
BstX2I RGATCY 2 cut(s) 222, 302
BstYI RGATCY 2 cut(s) 222, 302
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 3 cut(s) 108, 503, 507
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 3 cut(s) 26, 160, 411
BtsIMutI CAGTG 2 cut(s) 473, 522
Cac8I GCNNGC 1 cut(s) 505
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 250
CviAII CATG 3 cut(s) 292, 341, 356
CviQI GTAC 1 cut(s) 250
DpnI GATC 5 cut(s) 165, 224, 290, 304, 348
DpnII GATC 5 cut(s) 163, 222, 288, 302, 346
EaeI YGGCCR 2 cut(s) 501, 505
Eam1104I CTCTTC 3 cut(s) 28, 207, 399
EarI CTCTTC 3 cut(s) 28, 207, 399
FaeI CATG 3 cut(s) 295, 344, 359
FaiI YATR 7 cut(s) 150, 152, 293, 321, 342, 357, 513
FatI CATG 3 cut(s) 291, 340, 355
Fnu4HI GCNGC 2 cut(s) 79, 492
FokI GGATG 3 cut(s) 13, 167, 398
FseI GGCCGGCC 1 cut(s) 507
Fsp4HI GCNGC 2 cut(s) 79, 492
GlaI GCGC 1 cut(s) 92
GluI GCNGC 2 cut(s) 79, 492
HaeIII GGCC 3 cut(s) 108, 503, 507
HapII CCGG 2 cut(s) 202, 504
HhaI GCGC 1 cut(s) 93
Hin1II CATG 3 cut(s) 295, 344, 359
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 3 cut(s) 207, 215, 257
HpaII CCGG 2 cut(s) 202, 504
HphI GGTGA 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 396
Hpy188III TCNNGA 3 cut(s) 67, 272, 361
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 43, 360
HpyCH4IV ACGT 2 cut(s) 60, 439
HpyCH4V TGCA 3 cut(s) 122, 300, 494
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 78, 105, 500
HpySE526I ACGT 2 cut(s) 60, 439
Hsp92II CATG 3 cut(s) 295, 344, 359
HspAI GCGC 1 cut(s) 91
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
Kzo9I GATC 5 cut(s) 163, 222, 288, 302, 346
LpnPI CCDG 5 cut(s) 118, 215, 266, 346, 517
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 109
MaeII ACGT 2 cut(s) 60, 439
MalI GATC 5 cut(s) 165, 224, 290, 304, 348
MboI GATC 5 cut(s) 163, 222, 288, 302, 346
MboII GAAGA 9 cut(s) 15, 95, 140, 191, 224, 229, 298, 416, 431
MflI RGATCY 2 cut(s) 222, 302
MluCI AATT 3 cut(s) 52, 397, 496
MlyI GAGTC 1 cut(s) 224
MroNI GCCGGC 1 cut(s) 503
MspI CCGG 2 cut(s) 202, 504
MspR9I CCNGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 4 cut(s) 14, 78, 105, 500
NaeI GCCGGC 1 cut(s) 505
NciI CCSGG 1 cut(s) 203
NdeII GATC 5 cut(s) 163, 222, 288, 302, 346
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 3 cut(s) 295, 344, 359
NspI RCATGY 1 cut(s) 359
PciI ACATGT 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 207, 257
PkrI GCNGC 2 cut(s) 80, 493
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PscI ACATGT 1 cut(s) 355
PspPI GGNCC 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 2 cut(s) 222, 302
RigI GGCCGGCC 1 cut(s) 507
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SatI GCNGC 2 cut(s) 79, 492
Sau3AI GATC 5 cut(s) 163, 222, 288, 302, 346
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 203
SetI ASST 8 cut(s) 63, 74, 114, 148, 282, 442, 448, 536
SfaNI GCATC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 3 cut(s) 52, 397, 496
SsiI CCGC 1 cut(s) 78
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 201
TaiI ACGT 2 cut(s) 63, 442
TaqI TCGA 4 cut(s) 126, 210, 218, 260
TasI AATT 3 cut(s) 52, 397, 496
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 207, 257
TscAI CASTG 2 cut(s) 473, 529
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 15
TspGWI ACGGA 2 cut(s) 387, 404
TspRI CASTG 2 cut(s) 473, 529
XapI RAATTY 1 cut(s) 496
XceI RCATGY 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.