pycom02g03740

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013733
Physical Location & Seq
Reverse (-)
2497290 .. 2497826
537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGACAGCCTACGAAGCCTCCTCTTCATCCTCCTTCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTTAGCGGCGAAGACACGCGCAAGGGCTTCACGGGCCACCTCTACCATGCAATCGAAGACAGGGGATACAAGGCTTATATGGATGAGGACGATCTAAAAAGAGGGGAAGATATAAAAGAAGAACTCTACCGGGGAATCGAAGAGTCAATGATCTCTATCATTGTCTTCTCAAAGAAGTACGTGGATTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGGAGTGCAGATCCAAACTGGGGCGACATGTTTTGCCAATATTCTATCATGTCGATCCTTCACATGTCAGGAAGCAGGACGGAGATTTAGCCGAAGCATTTCAGAAGCACGAAGAGGGCATCAGTGAAGAAAAAGATGACAAGAAACGTGAAGCTAAACAAGAAAGAGTAAAACAGTGGAGAGAGGCTCTTACAAAAGCTGCAAATTTGTCTGGCCACCATCTTCAAATCACTGACAATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

179

Amino Acids

20.79

Weight (kDa)

5.92

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 174 3.8e-49 TIR domain
TIR_2 PF13676 21 - 125 5.7e-20 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AciI CCGC 1 cut(s) 78
AclWI GGATC 2 cut(s) 297, 341
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 1 cut(s) 496
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 312
AflIII ACRYGT 3 cut(s) 60, 319, 355
AgsI TTSAA 2 cut(s) 37, 518
AjiI CACGTC 1 cut(s) 61
AluBI AGCT 4 cut(s) 72, 280, 446, 491
AluI AGCT 4 cut(s) 72, 280, 446, 491
AlwI GGATC 2 cut(s) 297, 341
AoxI GGCC 2 cut(s) 106, 505
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 496
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 295
BalI TGGCCA 1 cut(s) 507
BbsI GAAGAC 3 cut(s) 90, 135, 229
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 519
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 2 cut(s) 79, 492
BlsI GCNGC 2 cut(s) 80, 493
Bme1390I CCNGG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 203
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 3 cut(s) 90, 135, 229
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 202
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse1I ACTGG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 2 cut(s) 26, 160
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 10
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 2 cut(s) 108, 507
BsiSI CCGG 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 312
BsnI GGCC 2 cut(s) 108, 507
Bsp143I GATC 5 cut(s) 163, 222, 288, 302, 346
BspACI CCGC 1 cut(s) 78
BspANI GGCC 2 cut(s) 108, 507
BspFNI CGCG 1 cut(s) 91
BspPI GGATC 2 cut(s) 297, 341
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 5 cut(s) 163, 222, 288, 302, 346
Bst4CI ACNGT 1 cut(s) 468
Bst6I CTCTTC 3 cut(s) 28, 207, 399
BstBAI YACGTR 1 cut(s) 253
BstF5I GGATG 2 cut(s) 26, 160
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 5 cut(s) 166, 225, 291, 305, 349
BstMBI GATC 5 cut(s) 163, 222, 288, 302, 346
BstMWI GCNNNNNNNGC 3 cut(s) 14, 78, 105
BstNSI RCATGY 2 cut(s) 323, 359
BstSCI CCNGG 1 cut(s) 201
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 3 cut(s) 90, 135, 229
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 2 cut(s) 108, 507
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 26, 160
BtsIMutI CAGTG 3 cut(s) 421, 473, 522
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 250
CviAII CATG 5 cut(s) 119, 292, 320, 341, 356
CviQI GTAC 1 cut(s) 250
DpnI GATC 5 cut(s) 165, 224, 290, 304, 348
DpnII GATC 5 cut(s) 163, 222, 288, 302, 346
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 3 cut(s) 28, 207, 399
EarI CTCTTC 3 cut(s) 28, 207, 399
FaeI CATG 5 cut(s) 122, 295, 323, 344, 359
FaiI YATR 8 cut(s) 120, 150, 152, 185, 293, 321, 342, 357
FatI CATG 5 cut(s) 118, 291, 319, 340, 355
Fnu4HI GCNGC 2 cut(s) 79, 492
FokI GGATG 2 cut(s) 13, 167
Fsp4HI GCNGC 2 cut(s) 79, 492
GlaI GCGC 1 cut(s) 92
GluI GCNGC 2 cut(s) 79, 492
HaeIII GGCC 2 cut(s) 108, 507
HapII CCGG 1 cut(s) 202
HhaI GCGC 1 cut(s) 93
Hin1II CATG 5 cut(s) 122, 295, 323, 344, 359
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 3 cut(s) 207, 215, 257
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 396
Hpy188III TCNNGA 3 cut(s) 67, 272, 361
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 43, 360
HpyCH4III ACNGT 1 cut(s) 468
HpyCH4IV ACGT 3 cut(s) 60, 252, 439
HpyCH4V TGCA 3 cut(s) 122, 300, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 78, 105
HpySE526I ACGT 3 cut(s) 60, 252, 439
Hsp92II CATG 5 cut(s) 122, 295, 323, 344, 359
HspAI GCGC 1 cut(s) 91
Kzo9I GATC 5 cut(s) 163, 222, 288, 302, 346
LpnPI CCDG 7 cut(s) 118, 215, 266, 296, 346, 353, 489
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 420
MaeII ACGT 3 cut(s) 60, 252, 439
MalI GATC 5 cut(s) 165, 224, 290, 304, 348
MboI GATC 5 cut(s) 163, 222, 288, 302, 346
MflI RGATCY 1 cut(s) 302
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 2 cut(s) 52, 496
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 8 cut(s) 28, 31, 40, 122, 151, 167, 400, 469
Mox20I TGGCCA 1 cut(s) 507
MroXI GAANNNNTTC 1 cut(s) 390
MscI TGGCCA 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 507
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 3 cut(s) 14, 78, 105
NciI CCSGG 1 cut(s) 203
NdeII GATC 5 cut(s) 163, 222, 288, 302, 346
NlaIII CATG 5 cut(s) 122, 295, 323, 344, 359
NspI RCATGY 2 cut(s) 323, 359
PciI ACATGT 2 cut(s) 319, 355
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 2 cut(s) 207, 257
PkrI GCNGC 2 cut(s) 80, 493
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 253
PscI ACATGT 2 cut(s) 319, 355
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SatI GCNGC 2 cut(s) 79, 492
Sau3AI GATC 5 cut(s) 163, 222, 288, 302, 346
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 203
SetI ASST 9 cut(s) 63, 74, 114, 255, 282, 442, 448, 493, 536
SfaNI GCATC 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 52, 496
SsiI CCGC 1 cut(s) 78
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 468
TaiI ACGT 3 cut(s) 63, 255, 442
TaqI TCGA 4 cut(s) 126, 210, 260, 345
TasI AATT 2 cut(s) 52, 496
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 207, 257
TscAI CASTG 3 cut(s) 421, 473, 529
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 1 cut(s) 15
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 3 cut(s) 421, 473, 529
XapI RAATTY 1 cut(s) 496
XceI RCATGY 2 cut(s) 323, 359
XmnI GAANNNNTTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.