pycom02g13940

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003099
Physical Location & Seq
Reverse (-)
10535981 .. 10540516
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
ATGAAGGGACCAAGAAGGATTGTTGCCCCCCGAAGGCAAAAGTTGGCGACGGAGGATTGTTTATCTGTAGCAGACCTAGCGGTTGGGTTTATGCAAATGCACCAAAGAACACATAGTATCCTTAAAGCAGTATATATACAAACACTATATCTACCACCAACGCACCAACGCACCATGGCCTGCGACAAAGGATTTTCGAGTAGCTATTTTCTACTGAAGCCAGAAGAAGTTGGCTTCTTTGATCTGGTTCACATTCTGTTCTCCTCCAACGTTGAGGAAGGAAGGTATGCAGATTACTCTAAGAGTGAGGGCACAGTCACAGATGAAACGATCAGCTTCAGACGAAGATGGCTTATGTTTATCTCCATCTTGGCTCAAAAGTTTCTGCAACTTGTGTCAAAGCCACTCTCAGTTTTGGGGTCGGCGACTGAAATGTGTTTGAACCTTCTGTCAAGCAACCGCAATATTTTTGTGCTCATGCTCAATTCCTTCCGAGGGAAGCTGGTAAAGCCAGATAAATCATCGGCAATATTTCTGTCATTTATAGGGAATATGGACAAGCGGGTGAAGTTAGACAGTAGCATCAAACATGATGATGCCAATTACCATGCAGCACTCTCTATGATGGCTTCTAAAGCTTCTTATGAAAACAAGACCTACATTGAAACTACTGTGAATGATCACTGGAAGATGGAGTTCTTGGGATTTTATGACTTCTGGAATGACTATCAACAAAAAGCCACAACACAAGCCTTCTTGCTTTGTGATAAAACTGGTGACCGTGACACCATTGTTGTGGCCTTTAGGGGAACTGAACCTTTCGATGCCGATGCATGGTGCTCCGACTTTGACATCTCATGGTACGAGCTTTCAGCTGGGGTTGGAAAAATTCATGGTGGCTTTATGAAAGCTTTAGGCTTACAAAAGAATGTTGGTTGGCCTAAAGAAATCAAAAAAGAAGATGGGCAGCCAGCCGTAGCTTACTATGCCATTAGAGAGATGCTAAAAGAAATTTTGAGCCAAAATGATCAAGCGAGATACATTGTGACAGGGCATAGTTTGGGTGGGGCTCTAGCAATTCTGTTTCCTGCAGTTCTGGCTCTGCACAATGAGGCATGGTTATTGGAGAGGTTGCAAGGGGTGTATACATTTGGGCAGCCAAGGGTAGGAGATGAGAAATTTGGAGAGTTCATGAGAGAAAAATTGAAAGAGCATGGTATTCGATATTTTAGATTTGTTTATGGTAACGATATGGTGCCTAGGTTGCCTTATGATGACAAGGACTTAATGTTCAAGCATTTTGGGACATGCCTCTACTATAATCGGCAGTATGAAGTGAAGGTTGTTGCAGAAGAACCGAACAAGAATTACTTTTCACCACTGGGGGCAATACCAATGATGATAAATGCCTTCTGCGAGCTGATTAGAAGCTTCACTATTTCACGCACAAAGGGACCAGACTATAAAGAAGGATCTTTTCTAAGAATTTTTAGGGTCATTGGCTTGGTACTTCCAGGCGTTCCAGCTCATAGCACCCAAGATTATGTTAATGCTACCCGGTTGGGATCATCAAACGTATTCCTCCCACCCAGAAACATACCGCAAGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

538

Amino Acids

61.31

Weight (kDa)

8.69

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 265 - 425 5.5e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1255
AccI GTMKAC 1 cut(s) 1145
AciI CCGC 4 cut(s) 80, 460, 562, 1601
AclI AACGTT 1 cut(s) 270
AclWI GGATC 2 cut(s) 1480, 1573
AcsI RAATTY 4 cut(s) 888, 1011, 1178, 1485
AcuI CTGAAG 2 cut(s) 236, 322
AfaI GTAC 2 cut(s) 863, 1509
AfiI CCNNNNNNNGG 4 cut(s) 33, 495, 834, 1166
AgsI TTSAA 4 cut(s) 442, 665, 1207, 1294
AjnI CCWGG 1 cut(s) 1513
AloI GAACNNNNNNTCC 2 cut(s) 1274, 1306
Alw21I GWGCWC 2 cut(s) 477, 842
AlwI GGATC 2 cut(s) 1480, 1573
AoxI GGCC 3 cut(s) 177, 798, 938
ApeKI GCWGC 3 cut(s) 611, 967, 1156
ApoI RAATTY 4 cut(s) 888, 1011, 1178, 1485
AspA2I CCTAGG 1 cut(s) 1259
AspS9I GGNCC 2 cut(s) 8, 1454
AsuC2I CCSGG 1 cut(s) 1558
AsuHPI GGTGA 3 cut(s) 577, 788, 1368
AvaII GGWCC 2 cut(s) 8, 1454
AvrII CCTAGG 1 cut(s) 1259
BaeGI GKGCMC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 1255
BanII GRGCYC 1 cut(s) 1072
Bbv12I GWGCWC 2 cut(s) 477, 842
BbvI GCAGC 3 cut(s) 623, 979, 1168
BccI CCATC 5 cut(s) 342, 374, 619, 685, 956
BceAI ACGGC 1 cut(s) 959
BcgI CGANNNNNNTGC 4 cut(s) 812, 846, 1202, 1236
BciT130I CCWGG 1 cut(s) 1515
BciVI GTATCC 1 cut(s) 128
BclI TGATCA 2 cut(s) 679, 1027
BcnI CCSGG 1 cut(s) 1558
BfaI CTAG 3 cut(s) 77, 1073, 1260
BfmI CTRYAG 2 cut(s) 66, 1089
BfuI GTATCC 1 cut(s) 128
BisI GCNGC 3 cut(s) 612, 968, 1157
BlnI CCTAGG 1 cut(s) 1259
BlsI GCNGC 3 cut(s) 613, 969, 1158
Bme1390I CCNGG 2 cut(s) 1515, 1558
Bme18I GGWCC 2 cut(s) 8, 1454
BmgT120I GGNCC 2 cut(s) 8, 1454
BmiI GGNNCC 3 cut(s) 9, 1257, 1455
BmrFI CCNGG 2 cut(s) 1515, 1558
BmrI ACTGGG 1 cut(s) 1391
BmsI GCATC 5 cut(s) 586, 591, 814, 820, 990
BmuI ACTGGG 1 cut(s) 1391
BpuMI CCSGG 1 cut(s) 1558
BsaJI CCNNGG 4 cut(s) 174, 493, 1160, 1259
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 495, 834, 1166
Bse1I ACTGG 3 cut(s) 689, 778, 1386
BseBI CCWGG 1 cut(s) 1515
BseDI CCNNGG 4 cut(s) 174, 493, 1160, 1259
BseLI CCNNNNNNNGG 4 cut(s) 33, 495, 834, 1166
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 689, 778, 1386
BseRI GAGGAG 1 cut(s) 253
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 3 cut(s) 623, 979, 1168
BseYI CCCAGC 1 cut(s) 875
BsgI GTGCAG 1 cut(s) 1088
BshFI GGCC 3 cut(s) 179, 800, 940
BshNI GGYRCC 1 cut(s) 1255
BsiHKAI GWGCWC 2 cut(s) 477, 842
BsiSI CCGG 1 cut(s) 1558
BslFI GGGAC 3 cut(s) 21, 1318, 1467
BslI CCNNNNNNNGG 4 cut(s) 33, 495, 834, 1166
BsmFI GGGAC 3 cut(s) 21, 1318, 1467
BsnI GGCC 3 cut(s) 179, 800, 940
Bsp1286I GDGCHC 4 cut(s) 314, 477, 842, 1072
Bsp143I GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 4 cut(s) 80, 460, 562, 1601
BspANI GGCC 3 cut(s) 179, 800, 940
BspCNI CTCAG 1 cut(s) 422
BspHI TCATGA 1 cut(s) 1191
BspLI GGNNCC 3 cut(s) 9, 1257, 1455
BspMAI CTGCAG 1 cut(s) 1093
BspPI GGATC 2 cut(s) 1480, 1573
BspT107I GGYRCC 1 cut(s) 1255
BsrI ACTGG 3 cut(s) 689, 778, 1386
BssECI CCNNGG 4 cut(s) 174, 493, 1160, 1259
BssMI GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
BssNAI GTATAC 1 cut(s) 1146
BssT1I CCWWGG 3 cut(s) 174, 1160, 1259
Bst1107I GTATAC 1 cut(s) 1146
Bst2UI CCWGG 1 cut(s) 1515
Bst4CI ACNGT 4 cut(s) 316, 578, 673, 782
BstC8I GCNNGC 3 cut(s) 181, 972, 1418
BstDEI CTNAG 3 cut(s) 300, 409, 1481
BstDSI CCRYGG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 776
BstKTI GATC 6 cut(s) 244, 333, 682, 1030, 1475, 1568
BstMBI GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
BstMWI GCNNNNNNNGC 6 cut(s) 77, 508, 635, 986, 1097, 1264
BstNI CCWGG 1 cut(s) 1515
BstNSI RCATGY 1 cut(s) 1311
BstPI GGTNACC 1 cut(s) 776
BstSCI CCNGG 2 cut(s) 1513, 1556
BstSFI CTRYAG 2 cut(s) 66, 1089
BstSLI GKGCMC 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 623, 979, 1168
BstX2I RGATCY 1 cut(s) 1472
BstXI CCANNNNNNTGG 1 cut(s) 796
BstYI RGATCY 1 cut(s) 1472
BstZ17I GTATAC 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 3 cut(s) 179, 800, 940
BtgI CCRYGG 1 cut(s) 174
BtsIMutI CAGTG 2 cut(s) 682, 1379
Cac8I GCNNGC 3 cut(s) 181, 972, 1418
CciI TCATGA 1 cut(s) 1191
Cfr13I GGNCC 2 cut(s) 8, 1454
Csp6I GTAC 2 cut(s) 862, 1508
CviQI GTAC 2 cut(s) 862, 1508
DdeI CTNAG 3 cut(s) 300, 409, 1481
DpnI GATC 6 cut(s) 243, 332, 681, 1029, 1474, 1567
DpnII GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
Eco130I CCWWGG 3 cut(s) 174, 1160, 1259
Eco24I GRGCYC 1 cut(s) 1072
Eco47I GGWCC 2 cut(s) 8, 1454
Eco57I CTGAAG 2 cut(s) 236, 322
Eco91I GGTNACC 1 cut(s) 776
EcoO65I GGTNACC 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 1513
EcoT14I CCWWGG 3 cut(s) 174, 1160, 1259
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 1072
ErhI CCWWGG 3 cut(s) 174, 1160, 1259
FalI AAGNNNNNCTT 2 cut(s) 1355, 1387
FaqI GGGAC 3 cut(s) 21, 1318, 1467
FauI CCCGC 1 cut(s) 555
FbaI TGATCA 2 cut(s) 679, 1027
FblI GTMKAC 1 cut(s) 1145
Fnu4HI GCNGC 3 cut(s) 612, 968, 1157
FriOI GRGCYC 1 cut(s) 1072
Fsp4HI GCNGC 3 cut(s) 612, 968, 1157
FspBI CTAG 3 cut(s) 77, 1073, 1260
GluI GCNGC 3 cut(s) 612, 968, 1157
GsaI CCCAGC 1 cut(s) 879
HaeIII GGCC 3 cut(s) 179, 800, 940
HapII CCGG 1 cut(s) 1558
HindIII AAGCTT 3 cut(s) 636, 909, 1429
HpaII CCGG 1 cut(s) 1558
HphI GGTGA 3 cut(s) 577, 788, 1368
Hpy166II GTNNAC 2 cut(s) 250, 1146
Hpy188I TCNGA 3 cut(s) 341, 494, 844
Hpy188III TCNNGA 2 cut(s) 718, 1192
Hpy8I GTNNAC 2 cut(s) 250, 1146
Hpy99I CGWCG 1 cut(s) 52
HpyCH4III ACNGT 4 cut(s) 316, 578, 673, 782
HpyCH4IV ACGT 2 cut(s) 270, 1575
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 508, 635, 986, 1097, 1264
HpyF3I CTNAG 3 cut(s) 300, 409, 1481
HpySE526I ACGT 2 cut(s) 270, 1575
Ksp22I TGATCA 2 cut(s) 679, 1027
Kzo9I GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
LmnI GCTCC 1 cut(s) 845
Lsp1109I GCAGC 3 cut(s) 623, 979, 1168
LweI GCATC 5 cut(s) 586, 591, 814, 820, 990
MaeI CTAG 3 cut(s) 77, 1073, 1260
MaeII ACGT 2 cut(s) 270, 1575
MaeIII GTNAC 5 cut(s) 316, 776, 782, 1045, 1244
MalI GATC 6 cut(s) 243, 332, 681, 1029, 1474, 1567
MboI GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
MboII GAAGA 5 cut(s) 236, 357, 700, 971, 1364
MflI RGATCY 1 cut(s) 1472
MhlI GDGCHC 4 cut(s) 314, 477, 842, 1072
MluCI AATT 9 cut(s) 484, 601, 888, 1011, 1077, 1178, 1202, 1366, 1485
MmeI TCCRAC 3 cut(s) 291, 862, 867
MnlI CCTC 9 cut(s) 46, 268, 274, 301, 488, 1105, 1122, 1322, 1592
Mph1103I ATGCAT 1 cut(s) 835
MseI TTAA 3 cut(s) 123, 1286, 1548
MslI CAYNNNNRTG 2 cut(s) 594, 794
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 1 cut(s) 1558
MspR9I CCNGG 2 cut(s) 1515, 1558
MvaI CCWGG 1 cut(s) 1515
MwoI GCNNNNNNNGC 6 cut(s) 77, 508, 635, 986, 1097, 1264
NciI CCSGG 1 cut(s) 1558
NcoI CCATGG 1 cut(s) 174
NdeII GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
NlaIV GGNNCC 3 cut(s) 9, 1257, 1455
NmuCI GTSAC 4 cut(s) 316, 776, 782, 1045
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 1 cut(s) 1311
PagI TCATGA 1 cut(s) 1191
PkrI GCNGC 3 cut(s) 613, 969, 1158
Psp1406I AACGTT 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 1513
PspEI GGTNACC 1 cut(s) 776
PspFI CCCAGC 1 cut(s) 875
PspGI CCWGG 1 cut(s) 1513
PspN4I GGNNCC 3 cut(s) 9, 1257, 1455
PspPI GGNCC 2 cut(s) 8, 1454
PsrI GAACNNNNNNTAC 2 cut(s) 1352, 1384
PstI CTGCAG 1 cut(s) 1093
PsuI RGATCY 1 cut(s) 1472
PvuII CAGCTG 1 cut(s) 875
RsaI GTAC 2 cut(s) 863, 1509
RsaNI GTAC 2 cut(s) 862, 1508
RseI CAYNNNNRTG 2 cut(s) 594, 794
SaqAI TTAA 3 cut(s) 123, 1286, 1548
SatI GCNGC 3 cut(s) 612, 968, 1157
Sau3AI GATC 6 cut(s) 241, 330, 679, 1027, 1472, 1565
Sau96I GGNCC 2 cut(s) 8, 1454
ScrFI CCNGG 2 cut(s) 1515, 1558
SduI GDGCHC 4 cut(s) 314, 477, 842, 1072
SfaNI GCATC 5 cut(s) 586, 591, 814, 820, 990
SfcI CTRYAG 2 cut(s) 66, 1089
SinI GGWCC 2 cut(s) 8, 1454
SmiMI CAYNNNNRTG 2 cut(s) 594, 794
Sse9I AATT 9 cut(s) 484, 601, 888, 1011, 1077, 1178, 1202, 1366, 1485
SsiI CCGC 4 cut(s) 80, 460, 562, 1601
SspI AATATT 2 cut(s) 466, 531
SspMI CTAG 3 cut(s) 77, 1073, 1260
StyD4I CCNGG 2 cut(s) 1513, 1556
StyI CCWWGG 3 cut(s) 174, 1160, 1259
TaaI ACNGT 4 cut(s) 316, 578, 673, 782
TaiI ACGT 2 cut(s) 273, 1578
TaqI TCGA 3 cut(s) 197, 822, 1222
TasI AATT 9 cut(s) 484, 601, 888, 1011, 1077, 1178, 1202, 1366, 1485
Tru1I TTAA 3 cut(s) 123, 1286, 1548
Tru9I TTAA 3 cut(s) 123, 1286, 1548
TscAI CASTG 2 cut(s) 689, 1386
TseFI GTSAC 4 cut(s) 316, 776, 782, 1045
TseI GCWGC 3 cut(s) 611, 967, 1156
Tsp45I GTSAC 4 cut(s) 316, 776, 782, 1045
TspDTI ATGAA 7 cut(s) 17, 339, 660, 881, 920, 1180, 1347
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 2 cut(s) 689, 1386
VpaK11BI GGWCC 2 cut(s) 8, 1454
XapI RAATTY 4 cut(s) 888, 1011, 1178, 1485
XceI RCATGY 1 cut(s) 1311
XmaJI CCTAGG 1 cut(s) 1259
XmiI GTMKAC 1 cut(s) 1145
XspI CTAG 3 cut(s) 77, 1073, 1260
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.