pycom02g14670

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003836
Physical Location & Seq
Forward (+)
11250178 .. 11253798
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2496 bp
ATGGAGATATCATTGTTCAAAGCACTTCTTAATAACATCTCCTCTTTTTTCCATTTATCATCTCATGACAACATAAACTTTGACCCGGTTTTGAAGTACTGCAAAAGAGCTGAAGAGATATTAAAATTGTTGAAGACTGTACTTGATGCCATTGCTGATTCTGAAATAGCTTCTTATGAACTGCTTAATAAGCCATTTGAAGAACTGGGTCAATATGTTGATGAGTTGAGGGAGCAAATTGAAGACTGGCAGCCATTGTTTAGTAAAGTCAAGCTTGTTCTTCAGGTTGAGTCATTGATATCAAAGATTTGGACTTCTGGCCTGGATATCTTTCAACTGTTGAAGACATCCCCGCAGCATCTTCCTGATGAATTGGGTTCAGCATCTCTTGAGCACTGCATACAAAAATTTAAGCACATGGGATATGAACAAATGTCAACTGTCATTAAAGATGCTATAAACGATCAAGTGGAGGGTGTTGGACCAAGCTCAGAGATCCTAGTGAAAATTGCAGAGGGCCTTAGCTTGAGGTCCAACCAGGAGATTCTTATTGAGGCTGTAGCACTTGAAAAGTTGAAGGAAAATGCTGAACAATCTGAAAAGATTGAGGAAGCAGAGTATATTGAGCATCTGATATCCCTTGTTACCTGTATGCACGAGCGCCTTATTACGATAAAGCAATCCGAGAGCTGCAGCCCCGTTCCAATACCTGCTGATTTCTGCTGCCCCCTCTCTCTGGAGCTGATGACGGATCCAGTGATCGTGGCATCAGGTCAAACCTACGAGAGGACTTTCATCAAGAACTGGATCGGTCTTGGCCTCACTGTTTGTCCAAAGACACGGCAGACTTTGGCTCACACCAATTTGATACCCAATTACACTGTGAAGGCACTAATTGCAAATTGGTGCGAGTCAAACAATGTGAAACTGCCTGATCCCGCTAAGGCAGCACACCTTCTCGGGCAAGCAGAGCCTGGTGCACCAAAGGATTCATCTATTTTTCCTCATTCCAGGGTCAGTCAATCGATGCCACCTGAATCAACTCGGTCTACTGGTTCACCCTCGAAGAACTTGTTCTCATCTGGTCCTCTGTATCAGGAGAGAAGTTCTCCATTGCATCCTCGTTCTACATCAGAAGGTTCCTTGCCTGGTATAGTTGGAACTAGGCAGGATTTGGATATTGAAAGAATATCACTAGCAAATTCTGAAGACAGGTCTGCTAACTTAGAAGAAAGGAGTACAGATCTAGATAGCCAACACTCTATGTCACCATCTCGAGATGAACTTCCTAATTTTATTGAAGTTGAGCAGTCATCTCAAAGCCATAATAGAACCGCGTCAGCTTCCAGCGTACTTTCCAATGCCAATTGTAGTCAAGGAACTCCAGTCAATGGCAATGGGGCCTTACAGGTATCGACCAGTCTGTCAGGCTACAGCAGTGATGCTTCTGGGGAGTTGAAATCAGAACCACAAACTGTTGCTACCTTGGCCCCCCAACAGAGACAAACTGAGTCGCCAACCAGAATAGTAGAGGCAAGACCTCGAAACCAAATGTGGCGCCGACCATCTAGCTTAATCCCGAGGATAGTTTCTTCTCCACCTATTGAAACAAGACCTGACCTTTCTGAACTTGAAGCCCAAGTTCGTAACCTGGTTGAGGACTTGAAAAGCACTTCACTTGATACACAAAGAGAGGCAACATTTCAACTCCGGTTACTTGCCAAACATAATATGGATAATCGGATTGTTATTGCAAGTTGTGGGGCCATTGGCTTGTTGGTGGATTTGCTCCGCTCTGCAGATACAAGAGTTCAGGAGAATGCTGTCACAGCACTTCTTAACTTATCAATCAATGATAACAACAAAACTGCAATTGCTACTGCCAATGCAATTGAACCTCTGATTCATGTCCTTGAGACAGGGAGTGCAGAGGCCAAGGAGAACTCAGCTGCCACTCTCTTCAGCCTTTCGGTGATCGAGGACAACAAGGTTCGCATTGGAAGGTCAGGGGCTATCAGGCCTCTGGTTGATTTATTGGGGAATGGGAGTCCGAGAGGGAGGAAAGATGCAGCCACAGCTTTGTTTAATTTGTCAATTTTTCATGAGAATAAGGGTCGAATTGTTCAAGCTGGTGCTGTTAAGTACCTAGTGGAGTTGATGGACCCAGCGGCTGGAATGGTTGACAAGGCAGTTGCTGTTTTGGCTAATCTTTCAACAATTCCTGATGGGAGGACAGCAATTGGTCAGGAGGGTGGGATCCCTGTTCTTGTCGAGGTTGTTGAATTGGGTTCTGCAAGAGGGAAGGAAAATGCAGCTGCTGCTCTTTTACAGCTCTGCACAAACAGTAATCGATATTGCAGTCAGGTTCTCCAAGAAGGAGCTGTCCCACCATTAGTAGTATTGTCACAATCTGGCACCCCAAGGGCCAAAGAGAAGGCGCAGACGCTCCTTAGCTACTTCAGAAACCATAGACAAGGTAATGCTGGTAGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

832

Amino Acids

90.59

Weight (kDa)

5.51

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 2 - 141 1.3e-61 PUB2-like, N-terminal domain
U-box PF04564 236 - 306 1.6e-21 U-box domain
ARM_PUB PF25598 547 - 823 2.7e-28 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 548 - 720 5.2e-09 ARMC5
Arm PF00514 579 - 616 6.8e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 661 - 698 2.1e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 692 - 818 2.5e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1423
Acc36I ACCTGC 1 cut(s) 718
AccB1I GGYRCC 2 cut(s) 1557, 2414
AccB7I CCANNNNNTGG 2 cut(s) 1391, 2171
AccBSI CCGCTC 1 cut(s) 1794
AccI GTMKAC 1 cut(s) 1049
AccII CGCG 1 cut(s) 1337
AciI CCGC 5 cut(s) 353, 939, 1335, 1792, 2168
AclWI GGATC 7 cut(s) 490, 746, 759, 815, 929, 2251, 2264
AcsI RAATTY 2 cut(s) 407, 1201
AcuI CTGAAG 5 cut(s) 132, 266, 1227, 1945, 2443
AcyI GRCGYC 1 cut(s) 1558
AfaI GTAC 5 cut(s) 98, 141, 1240, 1353, 2144
AfiI CCNNNNNNNGG 5 cut(s) 736, 786, 1391, 1657, 2171
AjnI CCWGG 6 cut(s) 321, 537, 973, 1010, 1147, 1650
Alw21I GWGCWC 2 cut(s) 396, 982
Alw26I GTCTC 2 cut(s) 1495, 1910
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 7 cut(s) 490, 746, 759, 815, 929, 2251, 2264
AlwNI CAGNNNCTG 4 cut(s) 974, 2171, 2195, 2318
Ama87I CYCGRG 3 cut(s) 959, 1275, 1579
AoxI GGCC 9 cut(s) 319, 517, 817, 1401, 1488, 1764, 1932, 2018, 2424
ApaLI GTGCAC 1 cut(s) 978
ApoI RAATTY 2 cut(s) 407, 1201
Asp700I GAANNNNTTC 1 cut(s) 1073
AspLEI GCGC 3 cut(s) 663, 1560, 2440
AspS9I GGNCC 9 cut(s) 482, 517, 531, 1085, 1401, 1489, 1764, 2161, 2424
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 1050, 1260, 1984
AvaI CYCGRG 3 cut(s) 959, 1275, 1579
AvaII GGWCC 4 cut(s) 482, 531, 1085, 2161
BaeGI GKGCMC 1 cut(s) 982
BaeI ACNNNNGTAYC 2 cut(s) 1794, 1827
BamHI GGATCC 2 cut(s) 751, 2256
BanI GGYRCC 2 cut(s) 1557, 2414
BauI CACGAG 1 cut(s) 656
BbsI GAAGAC 4 cut(s) 140, 249, 350, 1215
Bbv12I GWGCWC 2 cut(s) 396, 982
BccI CCATC 4 cut(s) 1279, 1573, 2152, 2219
BceAI ACGGC 1 cut(s) 857
BciT130I CCWGG 6 cut(s) 323, 539, 975, 1012, 1149, 1652
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 1495, 1910
BfaI CTAG 6 cut(s) 500, 1164, 1196, 1247, 1569, 2147
BfmI CTRYAG 4 cut(s) 558, 691, 1432, 1797
BfoI RGCGCY 2 cut(s) 664, 1561
BfuAI ACCTGC 1 cut(s) 718
BglII AGATCT 1 cut(s) 1243
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 7 cut(s) 86, 323, 539, 975, 1012, 1149, 1652
Bme18I GGWCC 4 cut(s) 482, 531, 1085, 2161
BmeT110I CYCGRG 3 cut(s) 959, 1275, 1579
BmgT120I GGNCC 9 cut(s) 482, 517, 531, 1085, 1401, 1489, 1764, 2161, 2424
BmiI GGNNCC 9 cut(s) 753, 1141, 1402, 1491, 1559, 1765, 2163, 2258, 2416
BmrFI CCNGG 7 cut(s) 86, 323, 539, 975, 1012, 1149, 1652
BmrI ACTGGG 1 cut(s) 215
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 4 cut(s) 140, 249, 350, 1215
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
BpmI CTGGAG 2 cut(s) 758, 1368
Bpu10I CCTNAGC 3 cut(s) 521, 942, 2450
BpuEI CTTGAG 3 cut(s) 410, 547, 1934
BpuMI CCSGG 1 cut(s) 86
Bsa29I ATCGAT 2 cut(s) 1025, 2350
BsaHI GRCGYC 1 cut(s) 1558
BsaJI CCNNGG 5 cut(s) 1011, 1485, 1580, 1935, 2420
BsaWI WCCGGW 1 cut(s) 1710
Bsc4I CCNNNNNNNGG 5 cut(s) 736, 786, 1391, 1657, 2171
Bse1I ACTGG 7 cut(s) 210, 251, 755, 809, 1057, 1385, 1419
Bse3DI GCAATG 3 cut(s) 150, 1112, 1402
BseBI CCWGG 6 cut(s) 323, 539, 975, 1012, 1149, 1652
BseCI ATCGAT 2 cut(s) 1025, 2350
BseDI CCNNGG 5 cut(s) 1011, 1485, 1580, 1935, 2420
BseGI GGATG 2 cut(s) 347, 1117
BseLI CCNNNNNNNGG 5 cut(s) 736, 786, 1391, 1657, 2171
BseMI GCAATG 3 cut(s) 150, 1112, 1402
BseMII CTCAG 3 cut(s) 504, 1500, 1959
BseNI ACTGG 7 cut(s) 210, 251, 755, 809, 1057, 1385, 1419
BseRI GAGGAG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 982
BseYI CCCAGC 1 cut(s) 2164
BsgI GTGCAG 2 cut(s) 1947, 2320
Bsh1236I CGCG 1 cut(s) 1337
BshFI GGCC 9 cut(s) 321, 519, 819, 1403, 1490, 1766, 1934, 2020, 2426
BshNI GGYRCC 2 cut(s) 1557, 2414
BshVI ATCGAT 2 cut(s) 1025, 2350
BsiHKAI GWGCWC 2 cut(s) 396, 982
BsiHKCI CYCGRG 3 cut(s) 959, 1275, 1579
BsiSI CCGG 2 cut(s) 86, 1711
BslFI GGGAC 1 cut(s) 2369
BslI CCNNNNNNNGG 5 cut(s) 736, 786, 1391, 1657, 2171
BsmAI GTCTC 2 cut(s) 1495, 1910
BsmFI GGGAC 1 cut(s) 2369
BsmI GAATGC 1 cut(s) 1825
BsnI GGCC 9 cut(s) 321, 519, 819, 1403, 1490, 1766, 1934, 2020, 2426
BsoBI CYCGRG 3 cut(s) 959, 1275, 1579
Bsp1286I GDGCHC 2 cut(s) 396, 982
Bsp143I GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
BspACI CCGC 5 cut(s) 353, 939, 1335, 1792, 2168
BspANI GGCC 9 cut(s) 321, 519, 819, 1403, 1490, 1766, 1934, 2020, 2426
BspCNI CTCAG 3 cut(s) 503, 1501, 1958
BspDI ATCGAT 2 cut(s) 1025, 2350
BspFNI CGCG 1 cut(s) 1337
BspHI TCATGA 2 cut(s) 64, 2101
BspLI GGNNCC 9 cut(s) 753, 1141, 1402, 1491, 1559, 1765, 2163, 2258, 2416
BspMAI CTGCAG 2 cut(s) 695, 1801
BspMI ACCTGC 1 cut(s) 718
BspPI GGATC 7 cut(s) 490, 746, 759, 815, 929, 2251, 2264
BspT107I GGYRCC 2 cut(s) 1557, 2414
BsrBI CCGCTC 1 cut(s) 1794
BsrDI GCAATG 3 cut(s) 150, 1112, 1402
BsrI ACTGG 7 cut(s) 210, 251, 755, 809, 1057, 1385, 1419
BssECI CCNNGG 5 cut(s) 1011, 1485, 1580, 1935, 2420
BssMI GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
BssNI GRCGYC 1 cut(s) 1558
BssSI CACGAG 1 cut(s) 656
BssT1I CCWWGG 3 cut(s) 1485, 1935, 2420
Bst2BI CACGAG 1 cut(s) 656
Bst2UI CCWGG 6 cut(s) 323, 539, 975, 1012, 1149, 1652
Bst4CI ACNGT 7 cut(s) 139, 339, 442, 826, 883, 1477, 2345
Bst6I CTCTTC 2 cut(s) 108, 1964
BstACI GRCGYC 1 cut(s) 1558
BstAPI GCANNNNNTGC 2 cut(s) 896, 2318
BstC8I GCNNGC 1 cut(s) 966
BstDEI CTNAG 7 cut(s) 490, 521, 942, 1225, 1509, 1945, 2450
BstENI CCTNNNNNAGG 2 cut(s) 784, 1655
BstF5I GGATG 2 cut(s) 347, 1117
BstFNI CGCG 1 cut(s) 1337
BstH2I RGCGCY 2 cut(s) 664, 1561
BstHHI GCGC 3 cut(s) 663, 1560, 2440
BstKTI GATC 9 cut(s) 466, 498, 754, 762, 810, 937, 1246, 1977, 2259
BstMAI GTCTC 2 cut(s) 1495, 1910
BstMBI GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
BstMWI GCNNNNNNNGC 9 cut(s) 190, 896, 947, 970, 1487, 1829, 2075, 2201, 2318
BstNI CCWGG 6 cut(s) 323, 539, 975, 1012, 1149, 1652
BstSCI CCNGG 7 cut(s) 84, 321, 537, 973, 1010, 1147, 1650
BstSFI CTRYAG 4 cut(s) 558, 691, 1432, 1797
BstSLI GKGCMC 1 cut(s) 982
BstUI CGCG 1 cut(s) 1337
BstV2I GAAGAC 4 cut(s) 140, 249, 350, 1215
BstX2I RGATCY 4 cut(s) 495, 751, 1243, 2256
BstYI RGATCY 4 cut(s) 495, 751, 1243, 2256
Bsu15I ATCGAT 2 cut(s) 1025, 2350
BsuRI GGCC 9 cut(s) 321, 519, 819, 1403, 1490, 1766, 1934, 2020, 2426
BsuTUI ATCGAT 2 cut(s) 1025, 2350
BtsCI GGATG 2 cut(s) 347, 1117
BtsI GCAGTG 2 cut(s) 394, 1444
BtsIMutI CAGTG 5 cut(s) 394, 762, 822, 879, 1444
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 1 cut(s) 966
CaiI CAGNNNCTG 4 cut(s) 974, 2171, 2195, 2318
CciI TCATGA 2 cut(s) 64, 2101
CfoI GCGC 3 cut(s) 663, 1560, 2440
Cfr13I GGNCC 9 cut(s) 482, 517, 531, 1085, 1401, 1489, 1764, 2161, 2424
ClaI ATCGAT 2 cut(s) 1025, 2350
CseI GACGC 2 cut(s) 1326, 2452
CsiI ACCWGGT 1 cut(s) 1650
Csp6I GTAC 5 cut(s) 97, 140, 1239, 1352, 2143
CviAII CATG 4 cut(s) 65, 418, 1907, 2102
CviQI GTAC 5 cut(s) 97, 140, 1239, 1352, 2143
DdeI CTNAG 7 cut(s) 490, 521, 942, 1225, 1509, 1945, 2450
DinI GGCGCC 1 cut(s) 1559
DpnI GATC 9 cut(s) 465, 497, 753, 761, 809, 936, 1245, 1976, 2258
DpnII GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
DrdI GACNNNNNNGTC 1 cut(s) 1423
DseDI GACNNNNNNGTC 1 cut(s) 1423
Eam1104I CTCTTC 2 cut(s) 108, 1964
EarI CTCTTC 2 cut(s) 108, 1964
Eco130I CCWWGG 3 cut(s) 1485, 1935, 2420
Eco147I AGGCCT 1 cut(s) 2020
Eco32I GATATC 4 cut(s) 9, 300, 328, 636
Eco47I GGWCC 4 cut(s) 482, 531, 1085, 2161
Eco57I CTGAAG 5 cut(s) 132, 266, 1227, 1945, 2443
Eco88I CYCGRG 3 cut(s) 959, 1275, 1579
EcoNI CCTNNNNNAGG 2 cut(s) 784, 1655
EcoO109I RGGNCCY 2 cut(s) 517, 1401
EcoRII CCWGG 6 cut(s) 321, 537, 973, 1010, 1147, 1650
EcoRV GATATC 4 cut(s) 9, 300, 328, 636
EcoT14I CCWWGG 3 cut(s) 1485, 1935, 2420
EgeI GGCGCC 1 cut(s) 1559
EheI GGCGCC 1 cut(s) 1559
ErhI CCWWGG 3 cut(s) 1485, 1935, 2420
FaeI CATG 4 cut(s) 68, 421, 1910, 2105
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 258, 290
FaqI GGGAC 1 cut(s) 2369
FatI CATG 4 cut(s) 64, 417, 1906, 2101
FauI CCCGC 2 cut(s) 360, 946
FblI GTMKAC 1 cut(s) 1049
FokI GGATG 2 cut(s) 334, 1104
FspBI CTAG 6 cut(s) 500, 1164, 1196, 1247, 1569, 2147
GlaI GCGC 3 cut(s) 662, 1559, 2439
GsaI CCCAGC 1 cut(s) 2168
GsuI CTGGAG 2 cut(s) 758, 1368
HaeII RGCGCY 2 cut(s) 664, 1561
HaeIII GGCC 9 cut(s) 321, 519, 819, 1403, 1490, 1766, 1934, 2020, 2426
HapII CCGG 2 cut(s) 86, 1711
HgaI GACGC 2 cut(s) 1326, 2452
HhaI GCGC 3 cut(s) 663, 1560, 2440
Hin1I GRCGYC 1 cut(s) 1558
Hin1II CATG 4 cut(s) 68, 421, 1910, 2105
Hin6I GCGC 3 cut(s) 661, 1558, 2438
HinP1I GCGC 3 cut(s) 661, 1558, 2438
HincII GTYRAC 2 cut(s) 438, 2182
HindII GTYRAC 2 cut(s) 438, 2182
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 9 cut(s) 158, 290, 544, 911, 989, 1037, 1511, 1903, 2047
HpaII CCGG 2 cut(s) 86, 1711
HphI GGTGA 3 cut(s) 1050, 1260, 1984
Hpy166II GTNNAC 5 cut(s) 438, 980, 1050, 1058, 2182
Hpy8I GTNNAC 5 cut(s) 438, 980, 1050, 1058, 2182
HpyAV CCTTC 8 cut(s) 571, 880, 965, 1130, 1995, 2296, 2369, 2428
HpyCH4III ACNGT 7 cut(s) 139, 339, 442, 826, 883, 1477, 2345
HpyF10VI GCNNNNNNNGC 9 cut(s) 190, 896, 947, 970, 1487, 1829, 2075, 2201, 2318
HpyF3I CTNAG 7 cut(s) 490, 521, 942, 1225, 1509, 1945, 2450
Hsp92I GRCGYC 1 cut(s) 1558
Hsp92II CATG 4 cut(s) 68, 421, 1910, 2105
HspAI GCGC 3 cut(s) 661, 1558, 2438
KasI GGCGCC 1 cut(s) 1557
Kzo9I GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
LmnI GCTCC 5 cut(s) 232, 739, 1794, 2378, 2451
MabI ACCWGGT 1 cut(s) 1650
MaeI CTAG 6 cut(s) 500, 1164, 1196, 1247, 1569, 2147
MaeIII GTNAC 6 cut(s) 643, 1266, 1646, 1713, 1825, 2403
MalI GATC 9 cut(s) 465, 497, 753, 761, 809, 936, 1245, 1976, 2258
MbiI CCGCTC 1 cut(s) 1794
MboI GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
MfeI CAATTG 4 cut(s) 1366, 1872, 1890, 2238
MflI RGATCY 4 cut(s) 495, 751, 1243, 2256
MhlI GDGCHC 2 cut(s) 396, 982
Mly113I GGCGCC 1 cut(s) 1558
MlyI GAGTC 4 cut(s) 299, 920, 1520, 2056
MmeI TCCRAC 3 cut(s) 460, 558, 1138
MroXI GAANNNNTTC 1 cut(s) 1073
MseI TTAA 9 cut(s) 30, 122, 186, 411, 447, 1574, 1839, 2085, 2139
MspA1I CMGCKG 3 cut(s) 1949, 2168, 2315
MspI CCGG 2 cut(s) 86, 1711
MspR9I CCNGG 7 cut(s) 86, 323, 539, 975, 1012, 1149, 1652
MunI CAATTG 4 cut(s) 1366, 1872, 1890, 2238
Mva1269I GAATGC 1 cut(s) 1825
MvaI CCWGG 6 cut(s) 323, 539, 975, 1012, 1149, 1652
MvnI CGCG 1 cut(s) 1337
MwoI GCNNNNNNNGC 9 cut(s) 190, 896, 947, 970, 1487, 1829, 2075, 2201, 2318
NarI GGCGCC 1 cut(s) 1558
NciI CCSGG 1 cut(s) 86
NdeII GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
NlaIII CATG 4 cut(s) 68, 421, 1910, 2105
NlaIV GGNNCC 9 cut(s) 753, 1141, 1402, 1491, 1559, 1765, 2163, 2258, 2416
NmuCI GTSAC 3 cut(s) 1266, 1825, 2403
PaeR7I CTCGAG 1 cut(s) 1275
PagI TCATGA 2 cut(s) 64, 2101
PceI AGGCCT 1 cut(s) 2020
PctI GAATGC 1 cut(s) 1825
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 5 cut(s) 158, 544, 989, 1037, 1903
PflMI CCANNNNNTGG 2 cut(s) 1391, 2171
PleI GAGTC 4 cut(s) 298, 919, 1519, 2055
PluTI GGCGCC 1 cut(s) 1561
PpsI GAGTC 4 cut(s) 298, 919, 1519, 2055
Psp6I CCWGG 6 cut(s) 321, 537, 973, 1010, 1147, 1650
PspFI CCCAGC 1 cut(s) 2164
PspGI CCWGG 6 cut(s) 321, 537, 973, 1010, 1147, 1650
PspN4I GGNNCC 9 cut(s) 753, 1141, 1402, 1491, 1559, 1765, 2163, 2258, 2416
PspPI GGNCC 9 cut(s) 482, 517, 531, 1085, 1401, 1489, 1764, 2161, 2424
PstI CTGCAG 2 cut(s) 695, 1801
PstNI CAGNNNCTG 4 cut(s) 974, 2171, 2195, 2318
PsuI RGATCY 4 cut(s) 495, 751, 1243, 2256
PvuII CAGCTG 2 cut(s) 1949, 2315
RsaI GTAC 5 cut(s) 98, 141, 1240, 1353, 2144
RsaNI GTAC 5 cut(s) 97, 140, 1239, 1352, 2143
SaqAI TTAA 9 cut(s) 30, 122, 186, 411, 447, 1574, 1839, 2085, 2139
Sau3AI GATC 9 cut(s) 463, 495, 751, 759, 807, 934, 1243, 1974, 2256
Sau96I GGNCC 9 cut(s) 482, 517, 531, 1085, 1401, 1489, 1764, 2161, 2424
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 4 cut(s) 299, 920, 1520, 2056
ScrFI CCNGG 7 cut(s) 86, 323, 539, 975, 1012, 1149, 1652
SduI GDGCHC 2 cut(s) 396, 982
SexAI ACCWGGT 1 cut(s) 1650
SfcI CTRYAG 4 cut(s) 558, 691, 1432, 1797
SfoI GGCGCC 1 cut(s) 1559
Sfr274I CTCGAG 1 cut(s) 1275
SinI GGWCC 4 cut(s) 482, 531, 1085, 2161
SlaI CTCGAG 1 cut(s) 1275
SmlI CTYRAG 4 cut(s) 389, 526, 1275, 1913
SmoI CTYRAG 4 cut(s) 389, 526, 1275, 1913
SseBI AGGCCT 1 cut(s) 2020
SsiI CCGC 5 cut(s) 353, 939, 1335, 1792, 2168
SspDI GGCGCC 1 cut(s) 1557
SspMI CTAG 6 cut(s) 500, 1164, 1196, 1247, 1569, 2147
StuI AGGCCT 1 cut(s) 2020
StyD4I CCNGG 7 cut(s) 84, 321, 537, 973, 1010, 1147, 1650
StyI CCWWGG 3 cut(s) 1485, 1935, 2420
TaaI ACNGT 7 cut(s) 139, 339, 442, 826, 883, 1477, 2345
TaqI TCGA 9 cut(s) 1025, 1064, 1276, 1415, 1543, 1977, 2116, 2271, 2350
TaqII GACCGA 2 cut(s) 800, 1035
TatI WGTACW 3 cut(s) 96, 139, 1238
TauI GCSGC 1 cut(s) 2171
TfiI GAWTC 5 cut(s) 158, 544, 989, 1037, 1903
Tru1I TTAA 9 cut(s) 30, 122, 186, 411, 447, 1574, 1839, 2085, 2139
Tru9I TTAA 9 cut(s) 30, 122, 186, 411, 447, 1574, 1839, 2085, 2139
TscAI CASTG 5 cut(s) 401, 762, 829, 886, 1444
TseFI GTSAC 3 cut(s) 1266, 1825, 2403
Tsp45I GTSAC 3 cut(s) 1266, 1825, 2403
TspDTI ATGAA 8 cut(s) 192, 384, 441, 784, 981, 1296, 1895, 2090
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 5 cut(s) 401, 762, 829, 886, 1444
Van91I CCANNNNNTGG 2 cut(s) 1391, 2171
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 4 cut(s) 482, 531, 1085, 2161
XagI CCTNNNNNAGG 2 cut(s) 784, 1655
XapI RAATTY 2 cut(s) 407, 1201
XbaI TCTAGA 1 cut(s) 1246
XcmI CCANNNNNNNNNTGG 2 cut(s) 1729, 1774
XhoI CTCGAG 1 cut(s) 1275
XmiI GTMKAC 1 cut(s) 1049
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 6 cut(s) 500, 1164, 1196, 1247, 1569, 2147
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.