pycom02g18150
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006635
Physical Location & Seq
Forward (+)
16458468 .. 16460247
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 834 bp
ATGGACGGGTCCAAAAGTCAATCCAAATCGATCAGCCAAGACTCCGATCCGCCCACGGCCCACCACTTCCAAAACGTCGTTGTCATGCGCCACGGCGACCGCATCGACAACTTCGAGCCCCTGTGGACATCGCAATCACCCAGGCCGTGGGACCCGCCTCTGATCGAAGAGGGCAAGCTCCGGGCCTTCTGCACGGGTACCAAGCTCCGATCTGATCTCGGGTTTCCAATACACCGCGTCTTGGTCTCGCCATTCGTGCGATGCGTCGAGACTGCCGTCCAAGTCGTCACTGCCCTTTCCGCCACCGAAGGCCCTTCTCTCTCCAAATACGAGAGCGACCAGCCCATCCCCGTCGACCCTTCTAAGCTTAAGGTCTCAATTGAATATGGGTTATGCGAGATGCTAAACAAAGAGGCGATTCACGGTGATTTGGCTCCAAAAGATGAGCAATGGGATTTCAACATACCAGATCTTGAGGCTATGTTCCCGGCTGGGACAGTTGATCAAACTGCGAAACGAGTGTACAAAGAGTTGCCCCATTGGGGAGAGAGCGTGGCGGGTGCGCGTGCTAGATATGCTCAGGTCATTGAGGCCCTTGCTGATAAATACCCTGCAGAAAACTTGCTCCTTGTCACTCATGGTGAAGGAGTTGGTTCTTCAGTTTCCGCATTCCTGGAGGGTGCAACAGTATACGAAGTAGAATACTGTGCATATTCAGAACTAAGAAGGCCCATCAAAGACCAGTCATCAGCTGCAGGAAAGTTTGAGGTAATCGGAAATCGTGGCCGGACTGGGGTAAGTTACTCACTCCTCGCCTCAGGAACAGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

278

Amino Acids

30.4

Weight (kDa)

5.16

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 168 - 225 6.9e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 147
AccI GTMKAC 2 cut(s) 354, 690
AccII CGCG 2 cut(s) 237, 565
AciI CCGC 7 cut(s) 50, 100, 155, 235, 300, 557, 666
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 2 cut(s) 199, 524
AfiI CCNNNNNNNGG 4 cut(s) 147, 241, 542, 793
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 2 cut(s) 383, 460
AjnI CCWGG 2 cut(s) 140, 672
AluBI AGCT 4 cut(s) 178, 205, 367, 752
AluI AGCT 4 cut(s) 178, 205, 367, 752
Alw26I GTCTC 3 cut(s) 250, 263, 379
AlwI GGATC 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 7 cut(s) 57, 143, 183, 310, 591, 728, 784
ApeKI GCWGC 1 cut(s) 752
Asp718I GGTACC 1 cut(s) 197
AspLEI GCGC 2 cut(s) 90, 565
AspS9I GGNCC 7 cut(s) 9, 58, 151, 183, 311, 592, 729
AsuC2I CCSGG 2 cut(s) 182, 488
AsuHPI GGTGA 3 cut(s) 129, 437, 653
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 2 cut(s) 9, 151
AxyI CCTNAGG 1 cut(s) 817
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 739
BccI CCATC 2 cut(s) 353, 740
BceAI ACGGC 4 cut(s) 72, 109, 130, 260
BciT130I CCWGG 2 cut(s) 142, 674
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 2 cut(s) 182, 488
BcoDI GTCTC 3 cut(s) 250, 263, 379
BfaI CTAG 1 cut(s) 570
BfmI CTRYAG 2 cut(s) 612, 753
BfrI CTTAAG 1 cut(s) 368
BglII AGATCT 1 cut(s) 469
BisI GCNGC 1 cut(s) 753
BlsI GCNGC 1 cut(s) 754
Bme1390I CCNGG 4 cut(s) 142, 182, 488, 674
Bme18I GGWCC 2 cut(s) 9, 151
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 7 cut(s) 9, 58, 151, 183, 311, 592, 729
BmiI GGNNCC 5 cut(s) 10, 152, 153, 199, 435
BmrFI CCNGG 4 cut(s) 142, 182, 488, 674
BmrI ACTGGG 1 cut(s) 801
BmsI GCATC 3 cut(s) 111, 251, 390
BmuI ACTGGG 1 cut(s) 801
BoxI GACNNNNGTC 1 cut(s) 275
BpmI CTGGAG 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 182, 488
Bsa29I ATCGAT 1 cut(s) 29
BsaI GGTCTC 2 cut(s) 250, 379
BsaJI CCNNGG 4 cut(s) 54, 91, 140, 146
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 241, 542, 793
Bse1I ACTGG 2 cut(s) 742, 796
Bse21I CCTNAGG 1 cut(s) 817
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 142, 674
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 4 cut(s) 54, 91, 140, 146
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 4 cut(s) 147, 241, 542, 793
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 593, 831
BseNI ACTGG 2 cut(s) 742, 796
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 1 cut(s) 739
BseYI CCCAGC 1 cut(s) 491
BsgI GTGCAG 1 cut(s) 175
Bsh1236I CGCG 2 cut(s) 237, 565
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 7 cut(s) 59, 145, 185, 312, 593, 730, 786
BshNI GGYRCC 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 181, 488, 787
BslFI GGGAC 2 cut(s) 164, 508
BslI CCNNNNNNNGG 4 cut(s) 147, 241, 542, 793
BsmAI GTCTC 3 cut(s) 250, 263, 379
BsmFI GGGAC 2 cut(s) 164, 508
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 7 cut(s) 59, 145, 185, 312, 593, 730, 786
Bso31I GGTCTC 2 cut(s) 250, 379
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
BspACI CCGC 7 cut(s) 50, 100, 155, 235, 300, 557, 666
BspANI GGCC 7 cut(s) 59, 145, 185, 312, 593, 730, 786
BspCNI CTCAG 2 cut(s) 592, 830
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 2 cut(s) 237, 565
BspLI GGNNCC 5 cut(s) 10, 152, 153, 199, 435
BspMAI CTGCAG 2 cut(s) 616, 757
BspPI GGATC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 2 cut(s) 250, 379
BsrDI GCAATG 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 2 cut(s) 742, 796
BssECI CCNNGG 4 cut(s) 54, 91, 140, 146
BssMI GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
BssNAI GTATAC 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 691
Bst2UI CCWGG 2 cut(s) 142, 674
Bst4CI ACNGT 4 cut(s) 425, 499, 688, 707
Bst6I CTCTTC 1 cut(s) 162
BstAFI CTTAAG 1 cut(s) 368
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 176, 567
BstDEI CTNAG 4 cut(s) 363, 579, 722, 817
BstDSI CCRYGG 3 cut(s) 54, 91, 146
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 2 cut(s) 237, 565
BstHHI GCGC 2 cut(s) 90, 565
BstKTI GATC 7 cut(s) 33, 49, 165, 212, 217, 472, 505
BstMAI GTCTC 3 cut(s) 250, 263, 379
BstMBI GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 3 cut(s) 256, 299, 575
BstNI CCWGG 2 cut(s) 142, 674
BstPAI GACNNNNGTC 1 cut(s) 275
BstSCI CCNGG 4 cut(s) 140, 180, 486, 672
BstSFI CTRYAG 2 cut(s) 612, 753
BstUI CGCG 2 cut(s) 237, 565
BstV1I GCAGC 1 cut(s) 739
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BstZ17I GTATAC 1 cut(s) 691
Bsu15I ATCGAT 1 cut(s) 29
Bsu36I CCTNAGG 1 cut(s) 817
BsuRI GGCC 7 cut(s) 59, 145, 185, 312, 593, 730, 786
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 3 cut(s) 54, 91, 146
BtgZI GCGATG 2 cut(s) 114, 274
BtsCI GGATG 1 cut(s) 345
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 176, 567
CfoI GCGC 2 cut(s) 90, 565
Cfr13I GGNCC 7 cut(s) 9, 58, 151, 183, 311, 592, 729
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 226, 253
Csp6I GTAC 2 cut(s) 198, 523
CviAII CATG 2 cut(s) 85, 638
CviQI GTAC 2 cut(s) 198, 523
DdeI CTNAG 4 cut(s) 363, 579, 722, 817
DpnI GATC 7 cut(s) 32, 48, 164, 211, 216, 471, 504
DpnII GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EciI GGCGGA 2 cut(s) 39, 289
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 2 cut(s) 250, 379
Eco47I GGWCC 2 cut(s) 9, 151
Eco57I CTGAAG 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 3 cut(s) 151, 311, 592
EcoRII CCWGG 2 cut(s) 140, 672
EcoT38I GRGCYC 1 cut(s) 120
FaeI CATG 2 cut(s) 88, 641
FaiI YATR 9 cut(s) 86, 387, 394, 464, 482, 576, 639, 691, 712
FaqI GGGAC 2 cut(s) 164, 508
FatI CATG 2 cut(s) 84, 637
FauI CCCGC 2 cut(s) 162, 550
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 2 cut(s) 354, 690
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 1 cut(s) 570
GlaI GCGC 2 cut(s) 89, 564
GluI GCNGC 1 cut(s) 753
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 7 cut(s) 59, 145, 185, 312, 593, 730, 786
HapII CCGG 3 cut(s) 181, 488, 787
HgaI GACGC 2 cut(s) 226, 253
HhaI GCGC 2 cut(s) 90, 565
Hin1II CATG 2 cut(s) 88, 641
Hin6I GCGC 2 cut(s) 88, 563
HinP1I GCGC 2 cut(s) 88, 563
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 2 cut(s) 41, 418
HpaII CCGG 3 cut(s) 181, 488, 787
HphI GGTGA 3 cut(s) 129, 437, 653
Hpy166II GTNNAC 4 cut(s) 126, 355, 523, 691
Hpy188I TCNGA 6 cut(s) 46, 162, 209, 214, 718, 776
Hpy188III TCNNGA 3 cut(s) 268, 473, 819
Hpy8I GTNNAC 4 cut(s) 126, 355, 523, 691
Hpy99I CGWCG 3 cut(s) 80, 269, 356
HpyAV CCTTC 6 cut(s) 196, 302, 324, 369, 638, 720
HpyCH4III ACNGT 4 cut(s) 425, 499, 688, 707
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 192, 614, 683, 710, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 299, 575
HpyF3I CTNAG 4 cut(s) 363, 579, 722, 817
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 2 cut(s) 88, 641
HspAI GCGC 2 cut(s) 88, 563
KflI GGGWCCC 1 cut(s) 151
KpnI GGTACC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
LmnI GCTCC 4 cut(s) 183, 210, 439, 630
Lsp1109I GCAGC 1 cut(s) 739
LweI GCATC 3 cut(s) 111, 251, 390
MaeI CTAG 1 cut(s) 570
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 286, 631, 800
MalI GATC 7 cut(s) 32, 48, 164, 211, 216, 471, 504
MboI GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
MboII GAAGA 2 cut(s) 179, 648
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 1 cut(s) 378
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 9 cut(s) 163, 168, 406, 469, 583, 670, 760, 821, 826
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 3 cut(s) 181, 488, 787
MspR9I CCNGG 4 cut(s) 142, 182, 488, 674
MunI CAATTG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 2 cut(s) 142, 674
MvnI CGCG 2 cut(s) 237, 565
MwoI GCNNNNNNNGC 3 cut(s) 256, 299, 575
NciI CCSGG 2 cut(s) 182, 488
NdeII GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
NlaIII CATG 2 cut(s) 88, 641
NlaIV GGNNCC 5 cut(s) 10, 152, 153, 199, 435
NmuCI GTSAC 2 cut(s) 286, 631
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 261, 273
PctI GAATGC 1 cut(s) 668
PfeI GAWTC 1 cut(s) 418
PflMI CCANNNNNTGG 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 672
PkrI GCNGC 1 cut(s) 754
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 151
PshAI GACNNNNGTC 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 140, 672
PspFI CCCAGC 1 cut(s) 491
PspGI CCWGG 2 cut(s) 140, 672
PspN4I GGNNCC 5 cut(s) 10, 152, 153, 199, 435
PspPI GGNCC 7 cut(s) 9, 58, 151, 183, 311, 592, 729
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 2 cut(s) 616, 757
PsuI RGATCY 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 2 cut(s) 199, 524
RsaNI GTAC 2 cut(s) 198, 523
SalI GTCGAC 1 cut(s) 353
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 7 cut(s) 30, 46, 162, 209, 214, 469, 502
Sau96I GGNCC 7 cut(s) 9, 58, 151, 183, 311, 592, 729
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 4 cut(s) 142, 182, 488, 674
SduI GDGCHC 1 cut(s) 120
SetI ASST 8 cut(s) 78, 180, 207, 369, 375, 585, 754, 771
SfaNI GCATC 3 cut(s) 111, 251, 390
SfcI CTRYAG 2 cut(s) 612, 753
SinI GGWCC 2 cut(s) 9, 151
SmlI CTYRAG 2 cut(s) 368, 473
SmoI CTYRAG 2 cut(s) 368, 473
Sse9I AATT 1 cut(s) 378
SsiI CCGC 7 cut(s) 50, 100, 155, 235, 300, 557, 666
SspMI CTAG 1 cut(s) 570
StyD4I CCNGG 4 cut(s) 140, 180, 486, 672
TaaI ACNGT 4 cut(s) 425, 499, 688, 707
TaiI ACGT 1 cut(s) 78
TaqI TCGA 6 cut(s) 29, 105, 114, 165, 267, 354
TasI AATT 1 cut(s) 378
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 286, 631
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 2 cut(s) 286, 631
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 147
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 2 cut(s) 9, 151
XmiI GTMKAC 2 cut(s) 354, 690
XspI CTAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.