pycom03g19910

asparagine--tRNA ligase, cytoplasmic 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005074
Physical Location & Seq
Reverse (-)
20535681 .. 20539398
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1710 bp
ATGGCTGATGATTCCACGCCTCCAAGTGCCCAGCTTGCTGCAGTAACCGTAAGCGACGATGCCTCCGTTCTAAAAGCGGAGTTTTCTGATAGAGTCCTCATCCGTTCCATTATTTCACGACCTGATGGAGGGTCTGGGTTGGCTGGGCAGAAAATTCGGATTGGTGGGTGGGTGAAGACTGGTCGCAAGGCAGACAAGGATGCATTTGCATTCCTGGAGCTGAATGATGGGTCATGCGCCAGCAACCTTCAGGTGATTGTGAAAGCTGACAAGTATGACCTTGGGCAGCTTGTGCAGACCGGTACTTGCCTGTCTGTGGATGGTGTGCTTAAGCTTCCCCCAGATGGGAAAAAACAGAAGGTGGAGCTTGATGTTGACAAGGTGATCCATGTGGGTCCGGTTGACCCAGCTAAATACCCATTGCCCAAGACCAGGCTCACCCTCGAGTTTTTGAGGGATCATGTTCATTTGCGTTCCAGAACCAACACTATCTCTGCAGTTACCCGAATCAGAAATGCATTGGCATTTGCAACCCACTCATTTTTCCATAAGCATGGATTTCTTTATGTGCAAACTCCAATTATCACCACTAGTGATTGTGAGGGTGCTGGTGAGATGTTTCAAGTCACAACCTCGTTTAGTGAAGCTGAAAAGTTGGAAAAGGATCTGATTGAGAACCCTCCTCCATCAGAAGCAGACATAGAAGCTGCCAAGCTTATCATCAAGGAGAGAGGAGATGATGTTGCTCAGTTGAAATCTGCTAAAGCAAGTAAGCAGGAAATTGGTGCTGCTGTGGCTGAGCTTAAAAAGGCAAAGGATAATGTCTTAAAGATGGAGGAGCGATCTAAGCTTAAACCTGGTATCCCTCAAAAGGATGGGAAGGTTGATTATACCCAAGATTTCTTTGCCCGTCAAGCCTTTTTGACTGTTTCTGGCCAATTACAAGTCGAATCTTATGCGTGTGCTCTTAGTAGCGTCTATACATTTGGGCCTACTTTTCGAGCTGAGAACTCCCATACTTCAAGGCATTTGGCAGAGTTCTGGATGGTGGAGCCAGAAATTGCATTTGCAGAACTTGAGGATGATATGAACTGTGCAGAGGCTTACGTGAAATTTCTGTGTCAGTGGTTACTTGACGACTGCAGGGAAGACATGCAATTTATGGCTGACAAAATTGATAAAACTTGCATTGATCGTCTAACAATGGTTGCTAAGACACCCTTTAAACGGATTACCTATACAGAAGAAGTAGATCTGCTAATTGATGCTGTGAAAAAGGGCAAGAAGTTTGAGAACCATGTAGAGTGGGGGATTGATTTGGCATCTGAGCACGAAAGATACCTGACAGAAGTATTATTTCAAAAACCTGTTATCGTTTACAACTATCCTAAAGGGATCAAAGCGTTCTACATGAGGCTCAATGATGATTTAAAAACAGTGGCTGCTATGGATGTTTTGGTACCAAAGGTGGGAGAGTTGATTGGGGGAAGCCAAAGGGAAGAGCGTTACGATGTTATTATGAGCAGGATTGAGGAAATGGGTCTCCCTGCTGAGCCATATGAGTGGTACCTTGACTTGCGGCGTTTTGGGACAGTCAAACATTGTGGTTTTGGGTTAGGATTCGAACGTATGGTTCTCTTTGCTACCGGGATTGACAACATCAGAGATGTTATTCCCTTCCCTAGATATCCAGGAAGAGCAGATCTTTGA

Protein Analysis

570

Amino Acids

63.74

Weight (kDa)

5.65

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 53 - 128 2.5e-07 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 152 - 212 3.6e-07 tRNA synthetases class II (D, K and N)
WHEP-TRS PF00458 241 - 274 4.4e-06 WHEP-TRS domain
tRNA-synt_2 PF00152 300 - 562 1.8e-49 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1459, 1566
AccB1I GGYRCC 2 cut(s) 1459, 1566
AciI CCGC 2 cut(s) 77, 1579
AclWI GGATC 4 cut(s) 379, 465, 672, 1403
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 2 cut(s) 153, 1112
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 3 cut(s) 304, 1461, 1568
AfiI CCNNNNNNNGG 8 cut(s) 128, 316, 344, 345, 432, 871, 1227, 1469
AflII CTTAAG 1 cut(s) 329
AgeI ACCGGT 1 cut(s) 299
AgsI TTSAA 4 cut(s) 623, 754, 1023, 1361
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 4 cut(s) 213, 431, 856, 1690
Alw21I GWGCWC 2 cut(s) 967, 1332
Alw26I GTCTC 1 cut(s) 1547
AlwI GGATC 4 cut(s) 379, 465, 672, 1403
AlwNI CAGNNNCTG 1 cut(s) 1442
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 2 cut(s) 934, 989
ApeKI GCWGC 5 cut(s) 38, 286, 707, 788, 1442
ApoI RAATTY 2 cut(s) 153, 1112
AsiGI ACCGGT 1 cut(s) 299
Asp718I GGTACC 2 cut(s) 1459, 1566
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 2 cut(s) 395, 989
AsuC2I CCSGG 1 cut(s) 1648
AsuHPI GGTGA 6 cut(s) 184, 265, 394, 430, 577, 623
AsuII TTCGAA 1 cut(s) 1623
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 2 cut(s) 1459, 1566
BbsI GAAGAC 2 cut(s) 182, 1155
Bbv12I GWGCWC 2 cut(s) 967, 1332
BbvI GCAGC 5 cut(s) 25, 298, 694, 775, 1429
BccI CCATC 8 cut(s) 119, 221, 314, 338, 694, 826, 869, 1039
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 938, 972
BciT130I CCWGG 4 cut(s) 215, 433, 858, 1692
BciVI GTATCC 1 cut(s) 872
BcnI CCSGG 1 cut(s) 1648
BcoDI GTCTC 1 cut(s) 1547
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 2 cut(s) 591, 1683
BfmI CTRYAG 3 cut(s) 39, 495, 1141
BfrI CTTAAG 1 cut(s) 329
BfuI GTATCC 1 cut(s) 872
BglII AGATCT 2 cut(s) 1252, 1702
BisI GCNGC 6 cut(s) 39, 287, 708, 789, 1443, 1580
BlpI GCTNAGC 2 cut(s) 798, 1551
BlsI GCNGC 6 cut(s) 40, 288, 709, 790, 1444, 1581
Bme1390I CCNGG 5 cut(s) 215, 433, 858, 1648, 1692
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 395, 989
BmiI GGNNCC 4 cut(s) 396, 1053, 1461, 1568
BmrFI CCNGG 5 cut(s) 215, 433, 858, 1648, 1692
BmsI GCATC 4 cut(s) 49, 190, 1255, 1331
BpiI GAAGAC 2 cut(s) 182, 1155
BpmI CTGGAG 1 cut(s) 236
Bpu1102I GCTNAGC 2 cut(s) 798, 1551
Bpu14I TTCGAA 1 cut(s) 1623
BpuEI CTTGAG 1 cut(s) 1097
BpuMI CCSGG 1 cut(s) 1648
BsaAI YACGTR 1 cut(s) 1108
BsaI GGTCTC 1 cut(s) 1547
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 299, 397
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 726, 756
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 316, 344, 345, 432, 871, 1227, 1469
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 419
BseBI CCWGG 4 cut(s) 215, 433, 858, 1692
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 7 cut(s) 99, 205, 325, 880, 1050, 1087, 1456
BseLI CCNNNNNNNGG 8 cut(s) 128, 316, 344, 345, 432, 871, 1227, 1469
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 5 cut(s) 761, 789, 996, 1317, 1542
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 3 cut(s) 672, 747, 851
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 5 cut(s) 25, 298, 694, 775, 1429
BseYI CCCAGC 3 cut(s) 30, 143, 406
BsgI GTGCAG 2 cut(s) 314, 1116
BshFI GGCC 2 cut(s) 936, 991
BshNI GGYRCC 2 cut(s) 1459, 1566
BshTI ACCGGT 1 cut(s) 299
BsiHKAI GWGCWC 2 cut(s) 967, 1332
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 3 cut(s) 300, 398, 1647
BslFI GGGAC 1 cut(s) 1603
BslI CCNNNNNNNGG 8 cut(s) 128, 316, 344, 345, 432, 871, 1227, 1469
BsmAI GTCTC 1 cut(s) 1547
BsmFI GGGAC 1 cut(s) 1603
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 936, 991
Bso31I GGTCTC 1 cut(s) 1547
BsoBI CYCGRG 1 cut(s) 443
Bsp119I TTCGAA 1 cut(s) 1623
Bsp1286I GDGCHC 3 cut(s) 31, 967, 1332
Bsp143I GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
Bsp1720I GCTNAGC 2 cut(s) 798, 1551
BspACI CCGC 2 cut(s) 77, 1579
BspANI GGCC 2 cut(s) 936, 991
BspCNI CTCAG 5 cut(s) 760, 790, 997, 1318, 1543
BspLI GGNNCC 4 cut(s) 396, 1053, 1461, 1568
BspMAI CTGCAG 3 cut(s) 43, 499, 1145
BspPI GGATC 4 cut(s) 379, 465, 672, 1403
BspQI GCTCTTC 2 cut(s) 1494, 1690
BspT104I TTCGAA 1 cut(s) 1623
BspT107I GGYRCC 2 cut(s) 1459, 1566
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 1547
BsrDI GCAATG 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 4 cut(s) 215, 433, 858, 1692
Bst4CI ACNGT 5 cut(s) 49, 928, 1094, 1438, 1594
Bst6I CTCTTC 2 cut(s) 1494, 1690
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 1108
BstBI TTCGAA 1 cut(s) 1623
BstC8I GCNNGC 2 cut(s) 36, 241
BstDEI CTNAG 8 cut(s) 747, 798, 846, 968, 1005, 1212, 1326, 1551
BstENI CCTNNNNNAGG 1 cut(s) 126
BstF5I GGATG 7 cut(s) 99, 205, 325, 880, 1050, 1087, 1456
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 8 cut(s) 387, 460, 667, 845, 1195, 1255, 1398, 1705
BstMAI GTCTC 1 cut(s) 1547
BstMBI GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
BstMWI GCNNNNNNNGC 5 cut(s) 35, 292, 794, 847, 914
BstNI CCWGG 4 cut(s) 215, 433, 858, 1692
BstNSI RCATGY 1 cut(s) 1156
BstSCI CCNGG 5 cut(s) 213, 431, 856, 1646, 1690
BstSFI CTRYAG 3 cut(s) 39, 495, 1141
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 5 cut(s) 25, 298, 694, 775, 1429
BstV2I GAAGAC 2 cut(s) 182, 1155
BstX2I RGATCY 3 cut(s) 664, 1252, 1702
BstXI CCANNNNNNTGG 2 cut(s) 554, 1563
BstYI RGATCY 3 cut(s) 664, 1252, 1702
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 2 cut(s) 936, 991
BtsCI GGATG 7 cut(s) 99, 205, 325, 880, 1050, 1087, 1456
BtsIMutI CAGTG 2 cut(s) 1130, 1443
Cac8I GCNNGC 2 cut(s) 36, 241
CaiI CAGNNNCTG 1 cut(s) 1442
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 395, 989
CseI GACGC 1 cut(s) 964
CsiI ACCWGGT 1 cut(s) 856
Csp6I GTAC 3 cut(s) 303, 1460, 1567
CspAI ACCGGT 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 1585, 1620
CviAII CATG 7 cut(s) 234, 389, 461, 554, 1153, 1298, 1411
CviQI GTAC 3 cut(s) 303, 1460, 1567
DdeI CTNAG 8 cut(s) 747, 798, 846, 968, 1005, 1212, 1326, 1551
DpnI GATC 8 cut(s) 386, 459, 666, 844, 1194, 1254, 1397, 1704
DpnII GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
DraI TTTAAA 2 cut(s) 1225, 1431
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 2 cut(s) 1494, 1690
EarI CTCTTC 2 cut(s) 1494, 1690
Eco130I CCWWGG 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 1547
Eco32I GATATC 1 cut(s) 1688
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoRII CCWGG 4 cut(s) 213, 431, 856, 1690
EcoRV GATATC 1 cut(s) 1688
EcoT14I CCWWGG 1 cut(s) 280
EcoT22I ATGCAT 2 cut(s) 205, 520
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 7 cut(s) 237, 392, 464, 557, 1156, 1301, 1414
FalI AAGNNNNNCTT 2 cut(s) 1205, 1237
FaqI GGGAC 1 cut(s) 1603
FatI CATG 7 cut(s) 233, 388, 460, 553, 1152, 1297, 1410
FauNDI CATATG 1 cut(s) 1558
Fnu4HI GCNGC 6 cut(s) 39, 287, 708, 789, 1443, 1580
FokI GGATG 7 cut(s) 86, 212, 332, 887, 1057, 1094, 1463
Fsp4HI GCNGC 6 cut(s) 39, 287, 708, 789, 1443, 1580
FspBI CTAG 2 cut(s) 591, 1683
GlaI GCGC 1 cut(s) 238
GluI GCNGC 6 cut(s) 39, 287, 708, 789, 1443, 1580
GsaI CCCAGC 3 cut(s) 34, 147, 410
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 2 cut(s) 936, 991
HapII CCGG 3 cut(s) 300, 398, 1647
HgaI GACGC 1 cut(s) 964
HhaI GCGC 1 cut(s) 239
Hin1II CATG 7 cut(s) 237, 392, 464, 557, 1156, 1301, 1414
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 2 cut(s) 376, 403
HindII GTYRAC 2 cut(s) 376, 403
HindIII AAGCTT 3 cut(s) 332, 713, 848
HinfI GANTC 5 cut(s) 11, 93, 507, 950, 1620
HpaII CCGG 3 cut(s) 300, 398, 1647
HphI GGTGA 6 cut(s) 184, 265, 394, 430, 577, 623
Hpy166II GTNNAC 3 cut(s) 376, 403, 1378
Hpy188I TCNGA 7 cut(s) 88, 159, 512, 669, 691, 1327, 1664
Hpy188III TCNNGA 3 cut(s) 117, 477, 1042
Hpy8I GTNNAC 3 cut(s) 376, 403, 1378
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 4 cut(s) 257, 352, 874, 1687
HpyCH4III ACNGT 5 cut(s) 49, 928, 1094, 1438, 1594
HpyCH4IV ACGT 2 cut(s) 1107, 1627
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 292, 794, 847, 914
HpyF3I CTNAG 8 cut(s) 747, 798, 846, 968, 1005, 1212, 1326, 1551
HpySE526I ACGT 2 cut(s) 1107, 1627
Hsp92II CATG 7 cut(s) 237, 392, 464, 557, 1156, 1301, 1414
HspAI GCGC 1 cut(s) 237
KpnI GGTACC 2 cut(s) 1463, 1570
Kzo9I GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
LguI GCTCTTC 2 cut(s) 1494, 1690
LmnI GCTCC 4 cut(s) 217, 364, 838, 1051
Lsp1109I GCAGC 5 cut(s) 25, 298, 694, 775, 1429
LweI GCATC 4 cut(s) 49, 190, 1255, 1331
MabI ACCWGGT 1 cut(s) 856
MaeI CTAG 2 cut(s) 591, 1683
MaeII ACGT 2 cut(s) 1107, 1627
MaeIII GTNAC 5 cut(s) 43, 499, 625, 1128, 1505
MalI GATC 8 cut(s) 386, 459, 666, 844, 1194, 1254, 1397, 1704
MboI GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
MboII GAAGA 5 cut(s) 187, 1160, 1256, 1511, 1707
MflI RGATCY 3 cut(s) 664, 1252, 1702
MhlI GDGCHC 3 cut(s) 31, 967, 1332
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 9 cut(s) 153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 636
Mox20I TGGCCA 1 cut(s) 936
Mph1103I ATGCAT 2 cut(s) 205, 520
MscI TGGCCA 1 cut(s) 936
MseI TTAA 6 cut(s) 330, 804, 827, 852, 1224, 1430
MslI CAYNNNNRTG 2 cut(s) 552, 1561
Msp20I TGGCCA 1 cut(s) 936
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 300, 398, 1647
MspR9I CCNGG 5 cut(s) 215, 433, 858, 1648, 1692
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 4 cut(s) 215, 433, 858, 1692
MwoI GCNNNNNNNGC 5 cut(s) 35, 292, 794, 847, 914
NciI CCSGG 1 cut(s) 1648
NdeI CATATG 1 cut(s) 1558
NdeII GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
NlaIII CATG 7 cut(s) 237, 392, 464, 557, 1156, 1301, 1414
NlaIV GGNNCC 4 cut(s) 396, 1053, 1461, 1568
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 2 cut(s) 205, 520
NspI RCATGY 1 cut(s) 1156
NspV TTCGAA 1 cut(s) 1623
PaeR7I CTCGAG 1 cut(s) 443
PciSI GCTCTTC 2 cut(s) 1494, 1690
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 11, 507, 950, 1620
PfoI TCCNGGA 2 cut(s) 213, 1690
PinAI ACCGGT 1 cut(s) 299
PkrI GCNGC 6 cut(s) 40, 288, 709, 790, 1444, 1581
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 1108
Psp6I CCWGG 4 cut(s) 213, 431, 856, 1690
PspFI CCCAGC 3 cut(s) 30, 143, 406
PspGI CCWGG 4 cut(s) 213, 431, 856, 1690
PspN4I GGNNCC 4 cut(s) 396, 1053, 1461, 1568
PspPI GGNCC 2 cut(s) 395, 989
PspXI VCTCGAGB 1 cut(s) 443
PstI CTGCAG 3 cut(s) 43, 499, 1145
PstNI CAGNNNCTG 1 cut(s) 1442
PsuI RGATCY 3 cut(s) 664, 1252, 1702
RsaI GTAC 3 cut(s) 304, 1461, 1568
RsaNI GTAC 3 cut(s) 303, 1460, 1567
RseI CAYNNNNRTG 2 cut(s) 552, 1561
SapI GCTCTTC 2 cut(s) 1494, 1690
SaqAI TTAA 6 cut(s) 330, 804, 827, 852, 1224, 1430
SatI GCNGC 6 cut(s) 39, 287, 708, 789, 1443, 1580
Sau3AI GATC 8 cut(s) 384, 457, 664, 842, 1192, 1252, 1395, 1702
Sau96I GGNCC 2 cut(s) 395, 989
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 5 cut(s) 215, 433, 858, 1648, 1692
SduI GDGCHC 3 cut(s) 31, 967, 1332
SexAI ACCWGGT 1 cut(s) 856
SfaNI GCATC 4 cut(s) 49, 190, 1255, 1331
SfcI CTRYAG 3 cut(s) 39, 495, 1141
Sfr274I CTCGAG 1 cut(s) 443
SfuI TTCGAA 1 cut(s) 1623
SinI GGWCC 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 2 cut(s) 552, 1561
SmlI CTYRAG 3 cut(s) 329, 443, 1076
SmoI CTYRAG 3 cut(s) 329, 443, 1076
SpeI ACTAGT 1 cut(s) 590
Sse9I AATT 9 cut(s) 153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
SsiI CCGC 2 cut(s) 77, 1579
SspMI CTAG 2 cut(s) 591, 1683
StyD4I CCNGG 5 cut(s) 213, 431, 856, 1646, 1690
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 5 cut(s) 49, 928, 1094, 1438, 1594
TaiI ACGT 2 cut(s) 1110, 1630
TaqI TCGA 4 cut(s) 444, 948, 1000, 1623
TasI AATT 9 cut(s) 153, 579, 780, 938, 1059, 1112, 1157, 1173, 1260
TauI GCSGC 1 cut(s) 1582
TfiI GAWTC 4 cut(s) 11, 507, 950, 1620
Tru1I TTAA 6 cut(s) 330, 804, 827, 852, 1224, 1430
Tru9I TTAA 6 cut(s) 330, 804, 827, 852, 1224, 1430
TscAI CASTG 2 cut(s) 1130, 1443
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 5 cut(s) 38, 286, 707, 788, 1442
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 2 cut(s) 455, 1103
TspGWI ACGGA 3 cut(s) 55, 92, 1243
TspRI CASTG 2 cut(s) 1130, 1443
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 395
XagI CCTNNNNNAGG 1 cut(s) 126
XapI RAATTY 2 cut(s) 153, 1112
XceI RCATGY 1 cut(s) 1156
XhoI CTCGAG 1 cut(s) 443
XspI CTAG 2 cut(s) 591, 1683
Zsp2I ATGCAT 2 cut(s) 205, 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.