pycom03g21820

Aldo-keto reductase-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006387
Physical Location & Seq
Forward (+)
22040800 .. 22042108
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21820.1

Sequence Viewer

Length: 645 bp
ATGGCGGTGCCGCTCGTCAACCTCGCCCCGAATCTAACAGTCTCAAAGCTCTGCTTGGGCACGACGACTTTCGGCGAGCAAAACTCGTTGCCGGAGTCCTTTCGACTCCTCGGCGGAGCTGTCGAGGCCGGAATCAACTTCTTCGACTCGGCAGAAATGTACCCGGTGGTTCAGCGAGCGGAGACTCAGGGGACGAGCGAGGAGTACCTCGGCCGCTGGATTAGGGAGAGCAAAACTCCTCGAAACAGCGTCGTTTTGGCCACCAAGTGTTTGGATGCCGGGAATATCGCCGACGCCATTGATGGCAGTTTAAGGCGGTTGCAGACGGATTACACCGATCTTTATCAGATTCATTGGCCCGATCGATTTGTATTTCCCATGTTTGGGGAAACTGAATGTGATCCAACGCGGCAGTTTTGTTCAATTCCTATGAAGGAACAACTGGATTCTCTTGAGAGAGCTGTTGATTCGGGTAAGATCAGATATGTTGGTCTCAGTAACGAAACACCATACGGTGTCATGAAGTTTGTTCAGGTATCTGAAAGGAGCACTTGCTGTCCAAAGATTGTATCTCTGCAGGTTGTCTATTTGATGGAATTCATACTCCCACTCAAATGGTTACTGTATTGCTCTATCGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

215

Amino Acids

24.12

Weight (kDa)

4.93

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 16 - 198 2.9e-39 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 7
AccBSI CCGCTC 2 cut(s) 13, 179
AccII CGCG 1 cut(s) 409
AciI CCGC 7 cut(s) 5, 11, 114, 179, 214, 316, 409
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 2 cut(s) 211, 258
AcsI RAATTY 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 294
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 2 cut(s) 161, 206
AfiI CCNNNNNNNGG 2 cut(s) 383, 384
AgsI TTSAA 1 cut(s) 423
AluBI AGCT 3 cut(s) 49, 119, 461
AluI AGCT 3 cut(s) 49, 119, 461
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 3 cut(s) 46, 176, 497
AlwI GGATC 1 cut(s) 395
AoxI GGCC 4 cut(s) 126, 211, 258, 356
ApoI RAATTY 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 2 cut(s) 164, 280
BaeGI GKGCMC 1 cut(s) 62
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 551
BccI CCATC 2 cut(s) 296, 586
BcnI CCSGG 2 cut(s) 164, 280
BcoDI GTCTC 3 cut(s) 46, 176, 497
BfmI CTRYAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 568
BisI GCNGC 3 cut(s) 11, 214, 410
BlsI GCNGC 3 cut(s) 12, 215, 411
Bme1390I CCNGG 2 cut(s) 164, 280
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 164, 280
BmsI GCATC 1 cut(s) 265
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 220, 252
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 164, 280
Bsa29I ATCGAT 2 cut(s) 364, 636
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 2 cut(s) 109, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 384
Bse1I ACTGG 1 cut(s) 447
Bse8I GATNNNNATC 1 cut(s) 342
BseCI ATCGAT 2 cut(s) 364, 636
BseDI CCNNGG 2 cut(s) 109, 208
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 383, 384
BseMII CTCAG 2 cut(s) 200, 508
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 3 cut(s) 98, 215, 228
BseSI GKGCMC 1 cut(s) 62
BseX3I CGGCCG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 409
Bsh1285I CGRYCG 2 cut(s) 214, 364
BshFI GGCC 4 cut(s) 128, 213, 260, 358
BshNI GGYRCC 1 cut(s) 7
BshVI ATCGAT 2 cut(s) 364, 636
BsiEI CGRYCG 2 cut(s) 214, 364
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 4 cut(s) 92, 129, 164, 279
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 2 cut(s) 383, 384
BsmAI GTCTC 3 cut(s) 46, 176, 497
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 4 cut(s) 128, 213, 260, 358
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 2 cut(s) 62, 551
Bsp143I GATC 4 cut(s) 337, 361, 400, 477
BspACI CCGC 7 cut(s) 5, 11, 114, 179, 214, 316, 409
BspANI GGCC 4 cut(s) 128, 213, 260, 358
BspCNI CTCAG 2 cut(s) 199, 507
BspDI ATCGAT 2 cut(s) 364, 636
BspFNI CGCG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 579
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 497
BsrBI CCGCTC 2 cut(s) 13, 179
BsrI ACTGG 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 109, 208
BssMI GATC 4 cut(s) 337, 361, 400, 477
BssNI GRCGYC 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 40, 515, 624
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 77, 177
BstDEI CTNAG 2 cut(s) 186, 494
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 409
BstKTI GATC 4 cut(s) 340, 364, 403, 480
BstMAI GTCTC 3 cut(s) 46, 176, 497
BstMBI GATC 4 cut(s) 337, 361, 400, 477
BstMCI CGRYCG 2 cut(s) 214, 364
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 162, 278
BstSFI CTRYAG 1 cut(s) 575
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 1 cut(s) 409
BstXI CCANNNNNNTGG 2 cut(s) 271, 615
BstZI CGGCCG 1 cut(s) 211
Bsu15I ATCGAT 2 cut(s) 364, 636
BsuRI GGCC 4 cut(s) 128, 213, 260, 358
BsuTUI ATCGAT 2 cut(s) 364, 636
BtsCI GGATG 1 cut(s) 280
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 2 cut(s) 77, 177
CciI TCATGA 1 cut(s) 519
Cfr13I GGNCC 1 cut(s) 357
ClaI ATCGAT 2 cut(s) 364, 636
CseI GACGC 2 cut(s) 238, 302
Csp6I GTAC 2 cut(s) 160, 205
CviAII CATG 2 cut(s) 379, 520
CviJI RGCY 7 cut(s) 49, 119, 128, 213, 260, 358, 461
CviKI_1 RGCY 7 cut(s) 49, 119, 128, 213, 260, 358, 461
CviQI GTAC 2 cut(s) 160, 205
DdeI CTNAG 2 cut(s) 186, 494
DpnI GATC 4 cut(s) 339, 363, 402, 479
DpnII GATC 4 cut(s) 337, 361, 400, 477
DraIII CACNNNGTG 1 cut(s) 267
EaeI YGGCCR 2 cut(s) 211, 258
EagI CGGCCG 1 cut(s) 211
EciI GGCGGA 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 211
Eco31I GGTCTC 1 cut(s) 497
Eco52I CGGCCG 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 596
FaeI CATG 2 cut(s) 382, 523
FaiI YATR 6 cut(s) 380, 431, 486, 511, 521, 602
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 535, 567
FaqI GGGAC 1 cut(s) 205
FatI CATG 2 cut(s) 378, 519
Fnu4HI GCNGC 3 cut(s) 11, 214, 410
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 3 cut(s) 11, 214, 410
GluI GCNGC 3 cut(s) 11, 214, 410
HaeIII GGCC 4 cut(s) 128, 213, 260, 358
HapII CCGG 4 cut(s) 92, 129, 164, 279
HgaI GACGC 2 cut(s) 238, 302
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 382, 523
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 9 cut(s) 31, 95, 105, 132, 146, 184, 349, 446, 467
HpaII CCGG 4 cut(s) 92, 129, 164, 279
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 3 cut(s) 348, 482, 541
Hpy188III TCNNGA 2 cut(s) 452, 520
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 3 cut(s) 67, 254, 296
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 3 cut(s) 40, 515, 624
HpyCH4V TGCA 2 cut(s) 322, 577
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 186, 494
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 382, 523
Kzo9I GATC 4 cut(s) 337, 361, 400, 477
LmnI GCTCC 2 cut(s) 116, 546
LpnPI CCDG 9 cut(s) 105, 142, 173, 177, 202, 292, 428, 518, 563
LweI GCATC 1 cut(s) 265
MaeIII GTNAC 2 cut(s) 497, 618
MalI GATC 4 cut(s) 339, 363, 402, 479
MbiI CCGCTC 2 cut(s) 13, 179
MboI GATC 4 cut(s) 337, 361, 400, 477
MboII GAAGA 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 62, 551
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 2 cut(s) 423, 596
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 4 cut(s) 99, 104, 140, 178
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 6 cut(s) 32, 118, 119, 193, 218, 249
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MseI TTAA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 613
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 4 cut(s) 92, 129, 164, 279
MspR9I CCNGG 2 cut(s) 164, 280
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 125
NciI CCSGG 2 cut(s) 164, 280
NdeII GATC 4 cut(s) 337, 361, 400, 477
NlaIII CATG 2 cut(s) 382, 523
NlaIV GGNNCC 1 cut(s) 9
NmeAIII GCCGAG 3 cut(s) 90, 128, 189
PagI TCATGA 1 cut(s) 519
PfeI GAWTC 5 cut(s) 31, 132, 349, 446, 467
PkrI GCNGC 3 cut(s) 12, 215, 411
Ple19I CGATCG 1 cut(s) 364
PleI GAGTC 4 cut(s) 99, 103, 140, 178
PpsI GAGTC 4 cut(s) 99, 103, 140, 178
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 579
PvuI CGATCG 1 cut(s) 364
RsaI GTAC 2 cut(s) 161, 206
RsaNI GTAC 2 cut(s) 160, 205
RseI CAYNNNNRTG 1 cut(s) 613
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 3 cut(s) 11, 214, 410
Sau3AI GATC 4 cut(s) 337, 361, 400, 477
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 4 cut(s) 99, 104, 140, 178
ScrFI CCNGG 2 cut(s) 164, 280
SduI GDGCHC 2 cut(s) 62, 551
SetI ASST 7 cut(s) 24, 51, 121, 210, 463, 537, 582
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 613
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 2 cut(s) 423, 596
SsiI CCGC 7 cut(s) 5, 11, 114, 179, 214, 316, 409
StyD4I CCNGG 2 cut(s) 162, 278
TaaI ACNGT 3 cut(s) 40, 515, 624
TaqI TCGA 6 cut(s) 103, 123, 144, 241, 364, 636
TasI AATT 2 cut(s) 423, 596
TauI GCSGC 3 cut(s) 13, 216, 412
TfiI GAWTC 5 cut(s) 31, 132, 349, 446, 467
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 341, 446, 536, 589
TspGWI ACGGA 1 cut(s) 341
XapI RAATTY 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.