pycom04g02500

NifU-like protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011072
Physical Location & Seq
Forward (+)
2234079 .. 2235863
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGAGAGGGCTACGAAGATTGCTTGGACGAAGTCTTTCGTCTCACACAAGGTGCCAACCTCCTCGGCATCGCAATGGCACCCAGTTCATCTCTCGCCGAACCTATTTCCTATCTGCTCATTCAGCTCCCACGACGTCGTACCTGGCTCACAACAACTCCCCCTTTTCCGATGCATCTCCTTTCAATTCTCTGAAATGGGGCCTTCTCGGCGGGCAGAAGAGAAGCATGTTTATCCAAACTCAATCCACACCCAACCCTGCATCTCACATGTTTTATCCTGGGAAACCCGTTATGGAAGTTGGCAGTGCAGACTTCCCGAATGCTCGTTCGGCCATGAATTCACCACTTGCCAAAGCCCTTTATGGAATTGATGGAGTCACTCGAGTGTTTTATGGATCAGATTTCGTTACTGTAACAAAATCTGAAGATACTTCTTGGGATCTATTGAAGCCTGAAATCTTTGCTGCAATCATGGATTTTTATTCTTCTGGGCAGCCACTCTTTCTAGACGAAAAAACTGCATCAGCAATGGACACAGCTATTCAAGAAGTATGTCATCAAATTTCTCTCTGTGAACTTTATAAGTTTTGGGGCCAACATGTTAACAGAGCGATTAGATCAATGTTTTACCTTTGCTTTGAAGATATTTTCATTTTTCTTTAG

Protein Analysis

221

Amino Acids

24.85

Weight (kDa)

9.1

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nfu_N PF08712 78 - 164 2.1e-30 Scaffold protein Nfu/NifU N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51390 AT3G20970 AT3G20970
fragaria_vesca FvH4_1g23510 FvH4_1g23510
malus_domestica MD04G1028700.v1.1
prunus_persica Prupe.2G079800_v2.0.a1
pyrus_communis pycom04g02500
rosa_chinensis RchiOBHm_Chr2g0118911
rosa_laevigata RLG00000018435
rosa_multiflora Rmu_sc0001653.1_g000031 Rmu_sc0003492.1_g000006
rosa_roxburghii Rroxscaffold_2G00124590 Rroxscaffold_2G00144600
rosa_rugosa Rorug02G0219500
rosa_samantha Rh2AG276800 Rh2BG288500 Rh2DG283800 Rh2DG302900
rosa_wichuraiana Rw2G022050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AatII GACGTC 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 51, 77
AciI CCGC 1 cut(s) 210
AclWI GGATC 2 cut(s) 403, 447
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 2 cut(s) 337, 561
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 51
AfaI GTAC 1 cut(s) 140
AflIII ACRYGT 2 cut(s) 267, 598
AgsI TTSAA 4 cut(s) 184, 448, 545, 641
AjnI CCWGG 2 cut(s) 141, 277
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 2 cut(s) 125, 539
AluI AGCT 2 cut(s) 125, 539
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 2 cut(s) 403, 447
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 3 cut(s) 199, 330, 592
ApeKI GCWGC 2 cut(s) 464, 493
ApoI RAATTY 2 cut(s) 337, 561
Asp700I GAANNNNTTC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 199, 592
AsuHPI GGTGA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 381
BanI GGYRCC 2 cut(s) 51, 77
BbvI GCAGC 2 cut(s) 451, 505
BccI CCATC 1 cut(s) 365
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 2 cut(s) 143, 279
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 207
BisI GCNGC 2 cut(s) 465, 494
BlsI GCNGC 2 cut(s) 466, 495
Bme1390I CCNGG 2 cut(s) 143, 279
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 199, 592
BmiI GGNNCC 4 cut(s) 53, 79, 200, 593
BmrFI CCNGG 2 cut(s) 143, 279
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 5 cut(s) 76, 160, 182, 269, 530
BmuI ACTGGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 62, 278
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 2 cut(s) 79, 534
BseBI CCWGG 2 cut(s) 143, 279
BseDI CCNNGG 2 cut(s) 62, 278
BseMI GCAATG 2 cut(s) 79, 534
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 451, 505
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 3 cut(s) 201, 332, 594
BshNI GGYRCC 2 cut(s) 51, 77
BsiHKCI CYCGRG 1 cut(s) 381
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 3 cut(s) 201, 332, 594
BsoBI CYCGRG 1 cut(s) 381
Bsp143I GATC 3 cut(s) 395, 439, 617
BspACI CCGC 1 cut(s) 210
BspANI GGCC 3 cut(s) 201, 332, 594
BspLI GGNNCC 4 cut(s) 53, 79, 200, 593
BspPI GGATC 2 cut(s) 403, 447
BspT107I GGYRCC 2 cut(s) 51, 77
BsrDI GCAATG 2 cut(s) 79, 534
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 62, 278
BssMI GATC 3 cut(s) 395, 439, 617
BssNI GRCGYC 1 cut(s) 134
Bst2UI CCWGG 2 cut(s) 143, 279
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 398, 442, 620
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 395, 439, 617
BstMWI GCNNNNNNNGC 3 cut(s) 122, 207, 329
BstNI CCWGG 2 cut(s) 143, 279
BstNSI RCATGY 3 cut(s) 229, 271, 602
BstSCI CCNGG 2 cut(s) 141, 277
BstV1I GCAGC 2 cut(s) 451, 505
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 3 cut(s) 201, 332, 594
BtgZI GCGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 199, 592
Csp6I GTAC 1 cut(s) 139
CviAII CATG 5 cut(s) 226, 268, 334, 472, 599
CviQI GTAC 1 cut(s) 139
DpnI GATC 3 cut(s) 397, 441, 619
DpnII GATC 3 cut(s) 395, 439, 617
DraIII CACNNNGTG 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 444
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 141, 277
EcoT22I ATGCAT 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 5 cut(s) 229, 271, 337, 475, 602
FatI CATG 5 cut(s) 225, 267, 333, 471, 598
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 2 cut(s) 465, 494
Fsp4HI GCNGC 2 cut(s) 465, 494
FspBI CTAG 1 cut(s) 506
GluI GCNGC 2 cut(s) 465, 494
HaeIII GGCC 3 cut(s) 201, 332, 594
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 5 cut(s) 229, 271, 337, 475, 602
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 1 cut(s) 375
HpaI GTTAAC 1 cut(s) 604
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 2 cut(s) 575, 604
Hpy188I TCNGA 4 cut(s) 169, 192, 400, 424
Hpy188III TCNNGA 3 cut(s) 316, 506, 545
Hpy8I GTNNAC 2 cut(s) 575, 604
Hpy99I CGWCG 2 cut(s) 136, 139
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 5 cut(s) 173, 260, 308, 467, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 207, 329
HpySE526I ACGT 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 5 cut(s) 229, 271, 337, 475, 602
KspAI GTTAAC 1 cut(s) 604
Kzo9I GATC 3 cut(s) 395, 439, 617
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 8 cut(s) 95, 128, 155, 264, 270, 291, 465, 474
Lsp1109I GCAGC 2 cut(s) 451, 505
LweI GCATC 5 cut(s) 76, 160, 182, 269, 530
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 3 cut(s) 376, 406, 412
MalI GATC 3 cut(s) 397, 441, 619
MboI GATC 3 cut(s) 395, 439, 617
MboII GAAGA 5 cut(s) 27, 229, 437, 477, 653
MflI RGATCY 1 cut(s) 439
MluCI AATT 4 cut(s) 184, 337, 366, 561
MlyI GAGTC 1 cut(s) 384
MnlI CCTC 2 cut(s) 69, 72
Mph1103I ATGCAT 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 1 cut(s) 603
MslI CAYNNNNRTG 2 cut(s) 72, 383
MspR9I CCNGG 2 cut(s) 143, 279
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 2 cut(s) 143, 279
MwoI GCNNNNNNNGC 3 cut(s) 122, 207, 329
NdeII GATC 3 cut(s) 395, 439, 617
NlaIII CATG 5 cut(s) 229, 271, 337, 475, 602
NlaIV GGNNCC 4 cut(s) 53, 79, 200, 593
NmeAIII GCCGAG 2 cut(s) 43, 186
NmuCI GTSAC 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 3 cut(s) 229, 271, 602
OliI CACNNNNGTG 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 381
PciI ACATGT 2 cut(s) 267, 598
PctI GAATGC 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 34
PflFI GACNNNGTC 1 cut(s) 30
PkrI GCNGC 2 cut(s) 466, 495
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PscI ACATGT 2 cut(s) 267, 598
PsiI TTATAA 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 141, 277
PspGI CCWGG 2 cut(s) 141, 277
PspN4I GGNNCC 4 cut(s) 53, 79, 200, 593
PspPI GGNCC 2 cut(s) 199, 592
PspXI VCTCGAGB 1 cut(s) 381
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 30
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 2 cut(s) 72, 383
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 2 cut(s) 465, 494
Sau3AI GATC 3 cut(s) 395, 439, 617
Sau96I GGNCC 2 cut(s) 199, 592
SchI GAGTC 1 cut(s) 384
ScrFI CCNGG 2 cut(s) 143, 279
SetI ASST 8 cut(s) 53, 61, 104, 127, 137, 144, 541, 633
SfaNI GCATC 5 cut(s) 76, 160, 182, 269, 530
Sfr274I CTCGAG 1 cut(s) 381
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 2 cut(s) 72, 383
SmlI CTYRAG 1 cut(s) 381
SmoI CTYRAG 1 cut(s) 381
Sse9I AATT 4 cut(s) 184, 337, 366, 561
SsiI CCGC 1 cut(s) 210
SspMI CTAG 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 141, 277
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 382
TasI AATT 4 cut(s) 184, 337, 366, 561
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 464, 493
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 76, 350, 640
TspRI CASTG 1 cut(s) 310
Tth111I GACNNNGTC 1 cut(s) 30
XapI RAATTY 2 cut(s) 337, 561
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 3 cut(s) 229, 271, 602
XhoI CTCGAG 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 1 cut(s) 506
ZraI GACGTC 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.