pycom04g04940

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004661
Physical Location & Seq
Reverse (-)
4567487 .. 4569465
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGACGTTAACTCTCCATTCTTCAACTTCGTTCGTCAATCCTAAAGACCACAAGGGCCTGAAAACCTCCGATGACTCCCCCGGCACGATATACTTTGCGCAAATCAAGCCATCATGCCGTCTCCGGGCAAAGAGTTCAATGCAAGAAACACAGCTCTTGCATGAGAAGAACTTAATGACTATCTCCGATGGAAGGAGAGAGTTGCTCCACGCACTCCCAAATACTGCTCACAGCAGCACTGACAACAAGGTGCCTGTGTATGTGATGCTCCCGCTCGACACGGTGAGTCACGGAGGGCATTTGAATAAGCCAAGAGCAATGAATGCGAGTTTGATGGCCCTGAAGCAAGCAGGGGTTGAAGGAGTTATGGTGGATGCGTGGTGGGGATTGGTGGAGAAGGACGGACCTTCCCAGTACAATTGGGAAGGGTATGATGAGCTTGTGCAGATGGTGCAAAAGCATGGCATGAAAATCCAAGTCGTCATGTCTTTTCATCAGTGTGGAGGAAATGTTGGAGACTCATGCAGCATTCCTCTACCTCCATGGGTGCTTGAAGAAATCAGCAAGAATCCTGACCTTGTTTACACAGACAAATCAGGCAGGAGGAATCCTGAGTACATATCCTTGGGATGCGATTCATTGCCTGTTCTCAGAGGAAGAACTCCCATTCAGGTCTACACCGATTACATGAGGAGCTTCCGCGACAGATTCATTGACTACTTGGGCGATGTTATTGTGGAAATTCAAGTAGGCATGGGTCCTTGTGGGGAGCTCAGATATCCAGCTTATCCAGAAAGCAACGGAACATGGAGGTTTCCTGGAATTGGAGAATTCCAATGCTATGACAAGTACATGAGAGCTTCCCTGGAAGCATCAGCAGAGGCACTGGGGAAGAGAGATTGGGGAAGAAGTGGACCACATGATTCCGGCCACTACAATCAATTTCCTGAAGACACAGGATTTTTCAAAAGGGATGGAACTTGGAACACTGAGTATGGACAGTTCTTCCTTGAATGGTACTCCGGAAAGCTACTAAGACACGGAGATAGAATACTGTCAGCTGCAAAAGGGGTATTCCAAGGATCATCCCAGTGGATCTAA

Protein Analysis

367

Amino Acids

41.25

Weight (kDa)

6.46

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 87 - 362 1.4e-146 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 250
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 675
AccII CGCG 1 cut(s) 702
AccIII TCCGGA 1 cut(s) 1022
AciI CCGC 2 cut(s) 272, 700
AclWI GGATC 1 cut(s) 1090
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 2 cut(s) 741, 830
AcuI CTGAAG 2 cut(s) 362, 969
AfaI GTAC 4 cut(s) 416, 617, 851, 1019
AfiI CCNNNNNNNGG 3 cut(s) 124, 192, 824
AgsI TTSAA 8 cut(s) 24, 138, 304, 359, 554, 746, 967, 1013
AjnI CCWGG 2 cut(s) 817, 864
AluBI AGCT 8 cut(s) 154, 439, 696, 772, 785, 860, 1030, 1061
AluI AGCT 8 cut(s) 154, 439, 696, 772, 785, 860, 1030, 1061
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 2 cut(s) 125, 510
AlwI GGATC 1 cut(s) 1090
Aor13HI TCCGGA 1 cut(s) 1022
AoxI GGCC 3 cut(s) 55, 336, 928
ApeKI GCWGC 3 cut(s) 234, 525, 1061
ApoI RAATTY 2 cut(s) 741, 830
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 5 cut(s) 55, 337, 404, 758, 914
AsuC2I CCSGG 2 cut(s) 81, 125
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 3 cut(s) 404, 758, 914
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 774
BbsI GAAGAC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 246, 537, 1048
BccI CCATC 5 cut(s) 118, 182, 328, 442, 968
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 819, 866
BcnI CCSGG 2 cut(s) 81, 125
BcoDI GTCTC 2 cut(s) 125, 510
BisI GCNGC 3 cut(s) 235, 526, 1062
BlsI GCNGC 3 cut(s) 236, 527, 1063
Bme1390I CCNGG 4 cut(s) 81, 125, 819, 866
Bme18I GGWCC 3 cut(s) 404, 758, 914
BmgT120I GGNCC 5 cut(s) 55, 337, 404, 758, 914
BmiI GGNNCC 2 cut(s) 252, 759
BmrFI CCNGG 4 cut(s) 81, 125, 819, 866
BmrI ACTGGG 3 cut(s) 406, 896, 1084
BmsI GCATC 4 cut(s) 255, 364, 620, 881
BmuI ACTGGG 3 cut(s) 406, 896, 1084
BpiI GAAGAC 1 cut(s) 957
BplI GAGNNNNNCTC 2 cut(s) 189, 221
BpuMI CCSGG 2 cut(s) 81, 125
BsaJI CCNNGG 5 cut(s) 79, 542, 624, 864, 1078
BsaWI WCCGGW 1 cut(s) 1022
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 495, 525
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 192, 824
Bse1I ACTGG 3 cut(s) 412, 891, 1090
Bse3DI GCAATG 2 cut(s) 324, 638
BseAI TCCGGA 1 cut(s) 1022
BseBI CCWGG 2 cut(s) 819, 866
BseDI CCNNGG 5 cut(s) 79, 542, 624, 864, 1078
BseGI GGATG 4 cut(s) 379, 635, 979, 1085
BseLI CCNNNNNNNGG 3 cut(s) 124, 192, 824
BseMI GCAATG 2 cut(s) 324, 638
BseMII CTCAG 4 cut(s) 603, 664, 787, 981
BseNI ACTGG 3 cut(s) 412, 891, 1090
BseRI GAGGAG 1 cut(s) 706
BseXI GCAGC 3 cut(s) 246, 537, 1048
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 702
BshFI GGCC 3 cut(s) 57, 338, 930
BshNI GGYRCC 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 4 cut(s) 81, 124, 927, 1023
BslI CCNNNNNNNGG 3 cut(s) 124, 192, 824
BsmAI GTCTC 2 cut(s) 125, 510
BsmBI CGTCTC 1 cut(s) 125
BsmI GAATGC 2 cut(s) 328, 528
BsnI GGCC 3 cut(s) 57, 338, 930
Bsp1286I GDGCHC 1 cut(s) 774
Bsp13I TCCGGA 1 cut(s) 1022
Bsp143I GATC 2 cut(s) 1082, 1095
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 2 cut(s) 272, 700
BspANI GGCC 3 cut(s) 57, 338, 930
BspCNI CTCAG 4 cut(s) 604, 663, 786, 982
BspEI TCCGGA 1 cut(s) 1022
BspFNI CGCG 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 252, 759
BspPI GGATC 1 cut(s) 1090
BspT107I GGYRCC 1 cut(s) 250
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 2 cut(s) 324, 638
BsrI ACTGG 3 cut(s) 412, 891, 1090
BssECI CCNNGG 5 cut(s) 79, 542, 624, 864, 1078
BssMI GATC 2 cut(s) 1082, 1095
BssT1I CCWWGG 3 cut(s) 542, 624, 1078
Bst2UI CCWGG 2 cut(s) 819, 866
Bst4CI ACNGT 3 cut(s) 283, 1002, 1056
Bst6I CTCTTC 1 cut(s) 887
BstAPI GCANNNNNTGC 2 cut(s) 323, 451
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 5 cut(s) 612, 650, 773, 990, 1034
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 4 cut(s) 379, 635, 979, 1085
BstFNI CGCG 1 cut(s) 702
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 2 cut(s) 1085, 1098
BstMAI GTCTC 2 cut(s) 125, 510
BstMBI GATC 2 cut(s) 1082, 1095
BstMWI GCNNNNNNNGC 3 cut(s) 106, 323, 451
BstNI CCWGG 2 cut(s) 819, 866
BstSCI CCNGG 4 cut(s) 79, 123, 817, 864
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 3 cut(s) 246, 537, 1048
BstV2I GAAGAC 1 cut(s) 957
BstX2I RGATCY 1 cut(s) 1095
BstYI RGATCY 1 cut(s) 1095
BsuRI GGCC 3 cut(s) 57, 338, 930
BtgI CCRYGG 1 cut(s) 542
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 4 cut(s) 379, 635, 979, 1085
BtsIMutI CAGTG 5 cut(s) 237, 503, 884, 987, 1097
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 5 cut(s) 55, 337, 404, 758, 914
Csp6I GTAC 4 cut(s) 415, 616, 850, 1018
CviQI GTAC 4 cut(s) 415, 616, 850, 1018
DdeI CTNAG 5 cut(s) 612, 650, 773, 990, 1034
DpnI GATC 2 cut(s) 1084, 1097
DpnII GATC 2 cut(s) 1082, 1095
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 3 cut(s) 542, 624, 1078
Eco24I GRGCYC 1 cut(s) 774
Eco32I GATATC 1 cut(s) 779
Eco47I GGWCC 3 cut(s) 404, 758, 914
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 2 cut(s) 362, 969
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 2 cut(s) 55, 758
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 2 cut(s) 817, 864
EcoRV GATATC 1 cut(s) 779
EcoT14I CCWWGG 3 cut(s) 542, 624, 1078
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 3 cut(s) 542, 624, 1078
Esp3I CGTCTC 1 cut(s) 125
FauI CCCGC 1 cut(s) 279
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 235, 526, 1062
FokI GGATG 4 cut(s) 386, 642, 986, 1072
FriOI GRGCYC 1 cut(s) 774
Fsp4HI GCNGC 3 cut(s) 235, 526, 1062
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 235, 526, 1062
HaeIII GGCC 3 cut(s) 57, 338, 930
HapII CCGG 4 cut(s) 81, 124, 927, 1023
HhaI GCGC 1 cut(s) 100
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 8 cut(s) 74, 286, 518, 568, 607, 635, 708, 923
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 4 cut(s) 81, 124, 927, 1023
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 4 cut(s) 9, 583, 676, 914
Hpy188I TCNGA 4 cut(s) 70, 187, 653, 776
Hpy188III TCNNGA 5 cut(s) 572, 611, 791, 947, 1023
Hpy8I GTNNAC 4 cut(s) 9, 583, 676, 914
HpyAV CCTTC 5 cut(s) 186, 353, 391, 417, 419
HpyCH4III ACNGT 3 cut(s) 283, 1002, 1056
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 6 cut(s) 142, 160, 445, 454, 525, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 323, 451
HpyF3I CTNAG 5 cut(s) 612, 650, 773, 990, 1034
HpySE526I ACGT 1 cut(s) 5
HspAI GCGC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 1022
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 2 cut(s) 1082, 1095
LmnI GCTCC 4 cut(s) 210, 273, 693, 769
Lsp1109I GCAGC 3 cut(s) 246, 537, 1048
LweI GCATC 4 cut(s) 255, 364, 620, 881
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 287
MalI GATC 2 cut(s) 1084, 1097
MbiI CCGCTC 1 cut(s) 274
MboI GATC 2 cut(s) 1082, 1095
MboII GAAGA 8 cut(s) 12, 178, 566, 669, 904, 918, 962, 997
MfeI CAATTG 1 cut(s) 418
MflI RGATCY 1 cut(s) 1095
MhlI GDGCHC 1 cut(s) 774
MluCI AATT 5 cut(s) 418, 741, 822, 830, 941
MlyI GAGTC 3 cut(s) 68, 295, 512
MmeI TCCRAC 1 cut(s) 493
MroI TCCGGA 1 cut(s) 1022
MseI TTAA 2 cut(s) 8, 173
MslI CAYNNNNRTG 2 cut(s) 498, 1090
MspA1I CMGCKG 1 cut(s) 1061
MspI CCGG 4 cut(s) 81, 124, 927, 1023
MspR9I CCNGG 4 cut(s) 81, 125, 819, 866
MunI CAATTG 1 cut(s) 418
Mva1269I GAATGC 2 cut(s) 328, 528
MvaI CCWGG 2 cut(s) 819, 866
MvnI CGCG 1 cut(s) 702
MwoI GCNNNNNNNGC 3 cut(s) 106, 323, 451
NciI CCSGG 2 cut(s) 81, 125
NcoI CCATGG 1 cut(s) 542
NdeII GATC 2 cut(s) 1082, 1095
NlaIV GGNNCC 2 cut(s) 252, 759
NmuCI GTSAC 1 cut(s) 287
NsbI TGCGCA 1 cut(s) 99
PctI GAATGC 2 cut(s) 328, 528
PfeI GAWTC 5 cut(s) 568, 607, 635, 708, 923
PfoI TCCNGGA 1 cut(s) 817
PkrI GCNGC 3 cut(s) 236, 527, 1063
PleI GAGTC 3 cut(s) 68, 294, 512
PpsI GAGTC 3 cut(s) 68, 294, 512
PpuMI RGGWCCY 1 cut(s) 758
Psp124BI GAGCTC 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 817, 864
PspGI CCWGG 2 cut(s) 817, 864
PspN4I GGNNCC 2 cut(s) 252, 759
PspPI GGNCC 5 cut(s) 55, 337, 404, 758, 914
PspPPI RGGWCCY 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 986, 1018
PsuI RGATCY 1 cut(s) 1095
PvuII CAGCTG 1 cut(s) 1061
RsaI GTAC 4 cut(s) 416, 617, 851, 1019
RsaNI GTAC 4 cut(s) 415, 616, 850, 1018
RseI CAYNNNNRTG 2 cut(s) 498, 1090
SacI GAGCTC 1 cut(s) 774
SaqAI TTAA 2 cut(s) 8, 173
SatI GCNGC 3 cut(s) 235, 526, 1062
Sau3AI GATC 2 cut(s) 1082, 1095
Sau96I GGNCC 5 cut(s) 55, 337, 404, 758, 914
SchI GAGTC 3 cut(s) 68, 295, 512
ScrFI CCNGG 4 cut(s) 81, 125, 819, 866
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 4 cut(s) 255, 364, 620, 881
SinI GGWCC 3 cut(s) 404, 758, 914
SmiMI CAYNNNNRTG 2 cut(s) 498, 1090
Sse9I AATT 5 cut(s) 418, 741, 822, 830, 941
SsiI CCGC 2 cut(s) 272, 700
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 4 cut(s) 79, 123, 817, 864
StyI CCWWGG 3 cut(s) 542, 624, 1078
TaaI ACNGT 3 cut(s) 283, 1002, 1056
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 276
TasI AATT 5 cut(s) 418, 741, 822, 830, 941
TatI WGTACW 3 cut(s) 414, 615, 849
TfiI GAWTC 5 cut(s) 568, 607, 635, 708, 923
Tru1I TTAA 2 cut(s) 8, 173
Tru9I TTAA 2 cut(s) 8, 173
TscAI CASTG 5 cut(s) 244, 503, 891, 994, 1097
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 3 cut(s) 234, 525, 1061
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 5 cut(s) 335, 482, 482, 627, 700
TspGWI ACGGA 4 cut(s) 306, 417, 816, 1056
TspRI CASTG 5 cut(s) 244, 503, 891, 994, 1097
VpaK11BI GGWCC 3 cut(s) 404, 758, 914
XapI RAATTY 2 cut(s) 741, 830
XmiI GTMKAC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.