pycom04g12300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004667
Physical Location & Seq
Reverse (-)
15278657 .. 15279940
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
ATGAAGGAGCTGCTCGGGAGACCTGGGTCCGTGAGCGGGTTGCTGCTGAGGGTATCGCAATTTTCATGTGCTGGTATCGCCATCGCCGTCATGGTCACCACCGACGGCTTCGCTGAATTCACTTGTTTCTGCTATTTGGTTGCGTCGATGGCACTTCAAGTGCTATGGAGCTTGGGGCTTGCTTGTCTTGATATTTATGCTTTGAGCAAAAAGAGAGACCTTCAAAATAAATTACTAGTGAGGCTATTTGTTGTCGGTGATTGGTTAACAGCTACTTTATCACTGGCTGCTGCAAGCTCTTCATCAGGGATTGCAATTCTGTTTGTAAGAGACTTTCATTTCTGCGCGGGTAGATGGGGTTTACCATGCGGTAGCTACAAGCTTGCAGTTTCCTTCACTTTTGTCACCTGGATCCTTACTTCCATATCCTTTCACGTGATGTTTTGGATCCTATCGGCATTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

156

Amino Acids

16.9

Weight (kDa)

8.85

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 139 1.1e-24 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 36
AccII CGCG 1 cut(s) 347
AciI CCGC 3 cut(s) 36, 347, 369
AclWI GGATC 4 cut(s) 406, 419, 442, 455
AcsI RAATTY 1 cut(s) 116
AcvI CACGTG 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 2 cut(s) 158, 224
AhlI ACTAGT 1 cut(s) 235
AjnI CCWGG 2 cut(s) 22, 407
AluBI AGCT 6 cut(s) 10, 171, 272, 297, 375, 382
AluI AGCT 6 cut(s) 10, 171, 272, 297, 375, 382
Alw26I GTCTC 3 cut(s) 13, 210, 324
AlwI GGATC 4 cut(s) 406, 419, 442, 455
Ama87I CYCGRG 1 cut(s) 14
ApeKI GCWGC 4 cut(s) 10, 43, 287, 290
ApoI RAATTY 1 cut(s) 116
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 3 cut(s) 88, 269, 397
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 27
BamHI GGATCC 2 cut(s) 411, 447
BbrPI CACGTG 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 30, 274, 277
BccI CCATC 3 cut(s) 89, 142, 348
BceAI ACGGC 2 cut(s) 71, 121
BciT130I CCWGG 2 cut(s) 24, 409
BcoDI GTCTC 3 cut(s) 13, 210, 324
BcuI ACTAGT 1 cut(s) 235
BfaI CTAG 1 cut(s) 236
BisI GCNGC 4 cut(s) 11, 44, 288, 291
BlsI GCNGC 4 cut(s) 12, 45, 289, 292
Bme1390I CCNGG 2 cut(s) 24, 409
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 28, 413, 449
BmrFI CCNGG 2 cut(s) 24, 409
BoxI GACNNNNGTC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 436
BsaI GGTCTC 2 cut(s) 13, 210
BsaJI CCNNGG 1 cut(s) 23
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 288
BseBI CCWGG 2 cut(s) 24, 409
BseDI CCNNGG 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 3 cut(s) 30, 274, 277
Bsh1236I CGCG 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 3 cut(s) 13, 210, 324
Bso31I GGTCTC 2 cut(s) 13, 210
BsoBI CYCGRG 1 cut(s) 14
Bsp143I GATC 2 cut(s) 411, 447
BspACI CCGC 3 cut(s) 36, 347, 369
BspCNI CTCAG 1 cut(s) 39
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 28, 413, 449
BspPI GGATC 4 cut(s) 406, 419, 442, 455
BspQI GCTCTTC 1 cut(s) 304
BspTNI GGTCTC 2 cut(s) 13, 210
BsrBI CCGCTC 1 cut(s) 36
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 2 cut(s) 411, 447
Bst2UI CCWGG 2 cut(s) 24, 409
Bst6I CTCTTC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 180, 295, 384
BstDEI CTNAG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 94
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 2 cut(s) 414, 450
BstMAI GTCTC 3 cut(s) 13, 210, 324
BstMBI GATC 2 cut(s) 411, 447
BstMWI GCNNNNNNNGC 2 cut(s) 77, 149
BstNI CCWGG 2 cut(s) 24, 409
BstPAI GACNNNNGTC 1 cut(s) 25
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 22, 407
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 30, 274, 277
BstX2I RGATCY 2 cut(s) 411, 447
BstYI RGATCY 2 cut(s) 411, 447
BtgZI GCGATG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 3 cut(s) 180, 295, 384
CfoI GCGC 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 1 cut(s) 132
CviAII CATG 3 cut(s) 66, 91, 366
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 413, 449
DpnII GATC 2 cut(s) 411, 447
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Eco31I GGTCTC 2 cut(s) 13, 210
Eco47I GGWCC 1 cut(s) 27
Eco72I CACGTG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 22, 407
FaeI CATG 3 cut(s) 69, 94, 369
FaiI YATR 6 cut(s) 67, 92, 166, 198, 367, 425
FatI CATG 3 cut(s) 65, 90, 365
FauI CCCGC 2 cut(s) 29, 340
Fnu4HI GCNGC 4 cut(s) 11, 44, 288, 291
Fsp4HI GCNGC 4 cut(s) 11, 44, 288, 291
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 346
GluI GCNGC 4 cut(s) 11, 44, 288, 291
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 347
Hin1II CATG 3 cut(s) 69, 94, 369
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 380
HpaI GTTAAC 1 cut(s) 267
HphI GGTGA 3 cut(s) 88, 269, 397
Hpy166II GTNNAC 2 cut(s) 267, 362
Hpy188III TCNNGA 2 cut(s) 16, 188
Hpy8I GTNNAC 2 cut(s) 267, 362
Hpy99I CGWCG 2 cut(s) 107, 148
HpyAV CCTTC 2 cut(s) 230, 403
HpyCH4IV ACGT 1 cut(s) 435
HpyCH4V TGCA 3 cut(s) 293, 314, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 149
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 435
Hsp92II CATG 3 cut(s) 69, 94, 369
HspAI GCGC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 267
Kzo9I GATC 2 cut(s) 411, 447
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 2 cut(s) 7, 168
LpnPI CCDG 7 cut(s) 9, 36, 57, 269, 291, 394, 421
Lsp1109I GCAGC 3 cut(s) 30, 274, 277
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 435
MaeIII GTNAC 2 cut(s) 94, 403
MalI GATC 2 cut(s) 413, 449
MbiI CCGCTC 1 cut(s) 36
MboI GATC 2 cut(s) 411, 447
MboII GAAGA 1 cut(s) 291
MflI RGATCY 2 cut(s) 411, 447
MluCI AATT 4 cut(s) 59, 116, 230, 315
MnlI CCTC 2 cut(s) 42, 234
MseI TTAA 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 24, 409
MvaI CCWGG 2 cut(s) 24, 409
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 77, 149
NdeII GATC 2 cut(s) 411, 447
NlaIII CATG 3 cut(s) 69, 94, 369
NlaIV GGNNCC 3 cut(s) 28, 413, 449
NmuCI GTSAC 2 cut(s) 94, 403
PciSI GCTCTTC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PkrI GCNGC 4 cut(s) 12, 45, 289, 292
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 436
PshAI GACNNNNGTC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 22, 407
PspCI CACGTG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 94
PspGI CCWGG 2 cut(s) 22, 407
PspN4I GGNNCC 3 cut(s) 28, 413, 449
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 2 cut(s) 411, 447
SapI GCTCTTC 1 cut(s) 304
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 4 cut(s) 11, 44, 288, 291
Sau3AI GATC 2 cut(s) 411, 447
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 2 cut(s) 24, 409
SinI GGWCC 1 cut(s) 27
SpeI ACTAGT 1 cut(s) 235
Sse9I AATT 4 cut(s) 59, 116, 230, 315
SsiI CCGC 3 cut(s) 36, 347, 369
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 22, 407
TaiI ACGT 1 cut(s) 438
TaqI TCGA 1 cut(s) 146
TasI AATT 4 cut(s) 59, 116, 230, 315
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 2 cut(s) 94, 403
TseI GCWGC 4 cut(s) 10, 43, 287, 290
Tsp45I GTSAC 2 cut(s) 94, 403
TspDTI ATGAA 4 cut(s) 17, 54, 291, 326
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 288
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.