pycom04g17710

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002647
Physical Location & Seq
Reverse (-)
19993218 .. 19995059
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGGCCCAGGAAGACTTGAGCGTGCCGGTGCAGCAACAGATGACCGAGGAGGAGGAACAGAGGCTCAAGTACCTCCAATTTGTTCAAGTGGCTGCAGCTCACGCTGCGGTTTGCTTCATAAACCTGTACGGTTTTGCCAAGGAAAGGTCGGGGCCTTTAAAGCCTGGTGTTGAGTCCGTGGAGGGAACAGTTAAGACCGTTGTTGGACCTGTCTACAACAAGTACCATGATGTCCCCGCTCACCTCCTCACCTTTGTGGACCACAAGGTTGATCAATCTGTTACCAAGCTGGACAGCCGTGTGCCACCACTGGTTAAGCAAGCCTCATCCAAAGCTCTATCCGCCGCTCAAAAGGCCCCAGAGGCAGCACGAGCGGTTGCCTCTGAGCTTAAGCGTGCAGGGGTTGTAGACACTGCCTCGAGCTGTGCTAAATCTGTCTACTCCAAGTGTGAACCCACAGCTAAGGACCTGTATGCCAAGTACGAACCAAAAGCCGAGCAATGTGCTGTTTCTGCTTGGCGAAAGGTGAACCAAGTCCTACCGAAGGTAGTTGATGTTGTTGTACCAACCGCTGCGTATTGTTCCGAAAAGTACAACCAGACTGTGCAGAGTACTGCGGAGAAGGGCTACAGGGTCTCCTCCTATCTGCCTTTGGTTCCTACGGAGAAAATTGCCAAGGTTTTCGCCGAGAAGGCACCCGAAGCAGAGCCTTTGCTTGCAAGTCACGGTGAAACTGAAGTCGCCACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

251

Amino Acids

27.18

Weight (kDa)

7.62

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 16 - 229 1.3e-89 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 694
AccBSI CCGCTC 3 cut(s) 239, 347, 374
AccI GTMKAC 3 cut(s) 213, 408, 438
AciI CCGC 7 cut(s) 107, 237, 342, 345, 374, 570, 617
AfaI GTAC 7 cut(s) 71, 128, 224, 482, 564, 593, 613
AfiI CCNNNNNNNGG 2 cut(s) 144, 544
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 2 cut(s) 6, 163
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 6 cut(s) 98, 289, 335, 388, 423, 461
AluI AGCT 6 cut(s) 98, 289, 335, 388, 423, 461
Alw26I GTCTC 1 cut(s) 640
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 3 cut(s) 3, 152, 354
ApeKI GCWGC 6 cut(s) 31, 92, 95, 104, 365, 572
AspS9I GGNCC 6 cut(s) 4, 152, 206, 259, 355, 466
AsuHPI GGTGA 4 cut(s) 233, 241, 538, 740
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 3 cut(s) 206, 259, 466
BanI GGYRCC 1 cut(s) 694
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 6 cut(s) 43, 79, 91, 107, 377, 559
BceAI ACGGC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 2 cut(s) 8, 165
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 640
BfmI CTRYAG 2 cut(s) 93, 628
BfrI CTTAAG 1 cut(s) 389
BglI GCCNNNNNGGC 2 cut(s) 362, 692
BisI GCNGC 7 cut(s) 32, 93, 96, 105, 345, 366, 573
BlsI GCNGC 7 cut(s) 33, 94, 97, 106, 346, 367, 574
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 2 cut(s) 8, 165
Bme18I GGWCC 3 cut(s) 206, 259, 466
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 6 cut(s) 4, 152, 206, 259, 355, 466
BmiI GGNNCC 4 cut(s) 153, 357, 657, 696
BmrFI CCNGG 2 cut(s) 8, 165
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 37, 50
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 5 cut(s) 6, 45, 138, 177, 675
BsaXI ACNNNNNCTCC 2 cut(s) 620, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 544
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 315
Bse3DI GCAATG 1 cut(s) 506
BseBI CCWGG 2 cut(s) 8, 165
BseDI CCNNGG 5 cut(s) 6, 45, 138, 177, 675
BseGI GGATG 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 144, 544
BseMI GCAATG 1 cut(s) 506
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 4 cut(s) 62, 65, 236, 628
BseXI GCAGC 6 cut(s) 43, 79, 91, 107, 377, 559
BsgI GTGCAG 3 cut(s) 50, 417, 626
BshFI GGCC 3 cut(s) 5, 154, 356
BshNI GGYRCC 1 cut(s) 694
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 144, 544
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 3 cut(s) 5, 154, 356
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 418
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 7 cut(s) 107, 237, 342, 345, 374, 570, 617
BspANI GGCC 3 cut(s) 5, 154, 356
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 153, 357, 657, 696
BspMAI CTGCAG 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 694
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 3 cut(s) 239, 347, 374
BsrDI GCAATG 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 6, 45, 138, 177, 675
BssMI GATC 1 cut(s) 271
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 138, 675
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 8, 165
Bst4CI ACNGT 5 cut(s) 131, 190, 199, 604, 728
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 4 cut(s) 23, 321, 396, 717
BstDEI CTNAG 2 cut(s) 384, 462
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 326
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 1 cut(s) 271
BstNI CCWGG 2 cut(s) 8, 165
BstSCI CCNGG 2 cut(s) 6, 163
BstSFI CTRYAG 2 cut(s) 93, 628
BstV1I GCAGC 6 cut(s) 43, 79, 91, 107, 377, 559
BstV2I GAAGAC 1 cut(s) 18
BsuRI GGCC 3 cut(s) 5, 154, 356
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 326
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 308, 411
Cac8I GCNNGC 4 cut(s) 23, 321, 396, 717
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 6 cut(s) 4, 152, 206, 259, 355, 466
Csp6I GTAC 7 cut(s) 70, 127, 223, 481, 563, 592, 612
CviAII CATG 1 cut(s) 227
CviQI GTAC 7 cut(s) 70, 127, 223, 481, 563, 592, 612
DdeI CTNAG 2 cut(s) 384, 462
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 159
EciI GGCGGA 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 138, 675
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 3 cut(s) 206, 259, 466
Eco88I CYCGRG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 3 cut(s) 152, 355, 466
EcoRII CCWGG 2 cut(s) 6, 163
EcoT14I CCWWGG 2 cut(s) 138, 675
ErhI CCWWGG 2 cut(s) 138, 675
FaeI CATG 1 cut(s) 230
FaiI YATR 3 cut(s) 119, 228, 474
FaqI GGGAC 1 cut(s) 218
FatI CATG 1 cut(s) 226
FauI CCCGC 1 cut(s) 244
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 3 cut(s) 213, 408, 438
Fnu4HI GCNGC 7 cut(s) 32, 93, 96, 105, 345, 366, 573
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 7 cut(s) 32, 93, 96, 105, 345, 366, 573
GluI GCNGC 7 cut(s) 32, 93, 96, 105, 345, 366, 573
HaeIII GGCC 3 cut(s) 5, 154, 356
HapII CCGG 1 cut(s) 26
Hin1II CATG 1 cut(s) 230
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 26
HphI GGTGA 4 cut(s) 233, 241, 538, 740
Hpy166II GTNNAC 6 cut(s) 214, 259, 409, 439, 452, 529
Hpy188I TCNGA 2 cut(s) 385, 586
Hpy8I GTNNAC 6 cut(s) 214, 259, 409, 439, 452, 529
HpyAV CCTTC 3 cut(s) 538, 616, 685
HpyCH4III ACNGT 5 cut(s) 131, 190, 199, 604, 728
HpyCH4V TGCA 5 cut(s) 31, 95, 398, 607, 719
HpyF3I CTNAG 2 cut(s) 384, 462
Hsp92II CATG 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 1 cut(s) 271
Lsp1109I GCAGC 6 cut(s) 43, 79, 91, 107, 377, 559
MaeIII GTNAC 2 cut(s) 280, 722
MalI GATC 1 cut(s) 273
MbiI CCGCTC 3 cut(s) 239, 347, 374
MboI GATC 1 cut(s) 271
MboII GAAGA 1 cut(s) 23
MluCI AATT 2 cut(s) 77, 669
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 184
MseI TTAA 4 cut(s) 158, 192, 315, 390
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 572
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 8, 165
MvaI CCWGG 2 cut(s) 8, 165
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 230
NlaIV GGNNCC 4 cut(s) 153, 357, 657, 696
NmeAIII GCCGAG 2 cut(s) 520, 712
NmuCI GTSAC 1 cut(s) 722
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 418
PkrI GCNGC 7 cut(s) 33, 94, 97, 106, 346, 367, 574
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 2 cut(s) 6, 163
PspGI CCWGG 2 cut(s) 6, 163
PspN4I GGNNCC 4 cut(s) 153, 357, 657, 696
PspPI GGNCC 6 cut(s) 4, 152, 206, 259, 355, 466
PspPPI RGGWCCY 1 cut(s) 466
PspXI VCTCGAGB 1 cut(s) 418
PstI CTGCAG 1 cut(s) 97
RsaI GTAC 7 cut(s) 71, 128, 224, 482, 564, 593, 613
RsaNI GTAC 7 cut(s) 70, 127, 223, 481, 563, 592, 612
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 4 cut(s) 158, 192, 315, 390
SatI GCNGC 7 cut(s) 32, 93, 96, 105, 345, 366, 573
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 6 cut(s) 4, 152, 206, 259, 355, 466
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 8, 165
SfcI CTRYAG 2 cut(s) 93, 628
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 3 cut(s) 206, 259, 466
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 4 cut(s) 16, 65, 389, 418
SmoI CTYRAG 4 cut(s) 16, 65, 389, 418
Sse9I AATT 2 cut(s) 77, 669
SsiI CCGC 7 cut(s) 107, 237, 342, 345, 374, 570, 617
StyD4I CCNGG 2 cut(s) 6, 163
StyI CCWWGG 2 cut(s) 138, 675
TaaI ACNGT 5 cut(s) 131, 190, 199, 604, 728
TaqI TCGA 1 cut(s) 419
TaqII GACCGA 1 cut(s) 59
TasI AATT 2 cut(s) 77, 669
TatI WGTACW 2 cut(s) 591, 611
TauI GCSGC 1 cut(s) 347
Tru1I TTAA 4 cut(s) 158, 192, 315, 390
Tru9I TTAA 4 cut(s) 158, 192, 315, 390
TscAI CASTG 2 cut(s) 315, 418
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 6 cut(s) 31, 92, 95, 104, 365, 572
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 2 cut(s) 166, 677
TspRI CASTG 2 cut(s) 315, 418
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 3 cut(s) 206, 259, 466
XagI CCTNNNNNAGG 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 3 cut(s) 213, 408, 438
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.