pycom04g19240

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001548
Physical Location & Seq
Reverse (-)
21023594 .. 21026246
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGACTAGTCGGGTGGTTACTCTATGGTATCGACCTCCCGAGCTCCTTCTTGGGGCTACTGATTACGGTGTGGGTGTGGACCTCTGGAGTGCAGGTTGCATTTTAGCGGAGTTACTAGCTGGGAAGCCTATCATGCCTGGTCGTACCGAGGTGGAGCAACTACATAAGATATACAAATTATGTGGTTCACCTTCAGATGAATACTGGAGGAAAGCAAAGTTGCCCAATGCAACGCTATTTAGGCCTCGGGAGCCTTACAGAAGATCCATAAGAGAGACATTCAAAGATTTTCCCCCAACTTCATTTCCCCTTATCGAAACTCTTCTTGCAATTGATCCAGCAGAACGTCAGACAGCCACAGCTGCATTGAGTAGTGTGTTTTTCCTGTCAGAGCCTTATGCATGTGAACCTTCTAGCCTCCCGAAGTATCCTCCAACCAAAGAAATAGACGCCAAACGCCGGGACGATGAAGCTCGTAGACTACGAGCTGCTGGTAGATCCCAGGCTGATGGTCCAAAGAAAACACGCACACGGGAACGGGCTCCTAGGAGTGGTATGCCGGCTCCAGAAGCCAATGCGGAGCTTCAGTCCAATATTGATAGAAGGCGCCTAATTACGCATGCAAATGCAAAGAGCAAAAGCGAAAAGTTTCCTCCACCACATCAGGATGGGGCAGTTGGCTACCCCTTGGGATCTTCACATCATTTTGATCCTGCCCTTGTTCCTCCTGATGTCCCTTTCAGTACAACCTCATTCAGTTATCCTAAAGAATCACTACAGGCTTGGTCAGGTCCGTTGGTTGACCCTGCTTCTGCCGGTGGTCCAAGACGGAAGAAACATACTGGACACGATGGGCGAGAAACTTCAAAATCCTATACAGGAAACCTTAGAGAGAAACATGACAATGCTCGTGTTAAGGGAAAGAGAAGTGCGGCTTGA

Protein Analysis

313

Amino Acids

34.64

Weight (kDa)

9.7

Isoelectric Point (pI)

63.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 128 7.8e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 606
AccI GTMKAC 1 cut(s) 478
AciI CCGC 3 cut(s) 107, 578, 932
AclWI GGATC 5 cut(s) 258, 329, 492, 700, 704
AcuI CTGAAG 2 cut(s) 177, 569
AcyI GRCGYC 2 cut(s) 450, 607
AfaI GTAC 2 cut(s) 145, 745
AfiI CCNNNNNNNGG 3 cut(s) 52, 459, 551
AgsI TTSAA 2 cut(s) 283, 867
AhlI ACTAGT 1 cut(s) 5
AjnI CCWGG 2 cut(s) 136, 501
AluBI AGCT 6 cut(s) 43, 119, 362, 473, 488, 583
AluI AGCT 6 cut(s) 43, 119, 362, 473, 488, 583
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 5 cut(s) 258, 329, 492, 700, 704
Ama87I CYCGRG 2 cut(s) 38, 246
AoxI GGCC 1 cut(s) 242
ApeKI GCWGC 2 cut(s) 362, 488
Asp700I GAANNNNTTC 2 cut(s) 321, 648
AspA2I CCTAGG 1 cut(s) 545
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 4 cut(s) 79, 512, 791, 821
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 180
AvaI CYCGRG 2 cut(s) 38, 246
AvaII GGWCC 4 cut(s) 79, 512, 791, 821
AvrII CCTAGG 1 cut(s) 545
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 2 cut(s) 45, 544
BauI CACGAG 1 cut(s) 909
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 2 cut(s) 349, 475
BccI CCATC 3 cut(s) 503, 662, 845
BciT130I CCWGG 2 cut(s) 138, 503
BciVI GTATCC 1 cut(s) 438
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 4 cut(s) 6, 116, 414, 546
BfmI CTRYAG 1 cut(s) 776
BfoI RGCGCY 1 cut(s) 610
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 1 cut(s) 438
BisI GCNGC 3 cut(s) 363, 489, 933
BlnI CCTAGG 1 cut(s) 545
BlsI GCNGC 3 cut(s) 364, 490, 934
Bme1390I CCNGG 3 cut(s) 138, 461, 503
Bme18I GGWCC 4 cut(s) 79, 512, 791, 821
BmeT110I CYCGRG 2 cut(s) 38, 246
BmgT120I GGNCC 4 cut(s) 79, 512, 791, 821
BmiI GGNNCC 4 cut(s) 252, 543, 564, 608
BmrFI CCNGG 3 cut(s) 138, 461, 503
BpmI CTGGAG 3 cut(s) 106, 226, 549
BpuMI CCSGG 1 cut(s) 461
BsaHI GRCGYC 2 cut(s) 450, 607
BsaJI CCNNGG 5 cut(s) 147, 245, 501, 545, 687
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 572, 602
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 459, 551
Bse118I RCCGGY 2 cut(s) 559, 815
Bse1I ACTGG 2 cut(s) 209, 847
BseBI CCWGG 2 cut(s) 138, 503
BseDI CCNNGG 5 cut(s) 147, 245, 501, 545, 687
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 3 cut(s) 52, 459, 551
BseNI ACTGG 2 cut(s) 209, 847
BseXI GCAGC 2 cut(s) 349, 475
BseYI CCCAGC 1 cut(s) 119
BsgI GTGCAG 1 cut(s) 111
BshFI GGCC 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 2 cut(s) 38, 246
BsiSI CCGG 3 cut(s) 460, 560, 816
BslFI GGGAC 2 cut(s) 476, 719
BslI CCNNNNNNNGG 3 cut(s) 52, 459, 551
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 476, 719
BsnI GGCC 1 cut(s) 244
BsoBI CYCGRG 2 cut(s) 38, 246
Bsp1286I GDGCHC 2 cut(s) 45, 544
Bsp143I GATC 5 cut(s) 263, 334, 497, 692, 709
BspACI CCGC 3 cut(s) 107, 578, 932
BspANI GGCC 1 cut(s) 244
BspLI GGNNCC 4 cut(s) 252, 543, 564, 608
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 5 cut(s) 258, 329, 492, 700, 704
BspT107I GGYRCC 1 cut(s) 606
BsrFI RCCGGY 2 cut(s) 559, 815
BsrI ACTGG 2 cut(s) 209, 847
BssAI RCCGGY 2 cut(s) 559, 815
BssECI CCNNGG 5 cut(s) 147, 245, 501, 545, 687
BssMI GATC 5 cut(s) 263, 334, 497, 692, 709
BssNI GRCGYC 2 cut(s) 450, 607
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 545, 687
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 138, 503
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 2 cut(s) 450, 607
BstC8I GCNNGC 2 cut(s) 561, 621
BstDEI CTNAG 1 cut(s) 887
BstF5I GGATG 1 cut(s) 673
BstH2I RGCGCY 1 cut(s) 610
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 5 cut(s) 266, 337, 500, 695, 712
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 5 cut(s) 263, 334, 497, 692, 709
BstMWI GCNNNNNNNGC 5 cut(s) 133, 241, 250, 362, 569
BstNI CCWGG 2 cut(s) 138, 503
BstNSI RCATGY 2 cut(s) 405, 623
BstSCI CCNGG 3 cut(s) 136, 459, 501
BstSFI CTRYAG 1 cut(s) 776
BstV1I GCAGC 2 cut(s) 349, 475
BstX2I RGATCY 3 cut(s) 263, 497, 692
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 3 cut(s) 263, 497, 692
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 673
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 561, 621
CfoI GCGC 1 cut(s) 609
Cfr10I RCCGGY 2 cut(s) 559, 815
Cfr13I GGNCC 4 cut(s) 79, 512, 791, 821
CseI GACGC 1 cut(s) 458
Csp6I GTAC 2 cut(s) 144, 744
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 4 cut(s) 133, 402, 620, 899
CviQI GTAC 2 cut(s) 144, 744
DdeI CTNAG 1 cut(s) 887
DinI GGCGCC 1 cut(s) 608
DpnI GATC 5 cut(s) 265, 336, 499, 694, 711
DpnII GATC 5 cut(s) 263, 334, 497, 692, 709
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 545, 687
Eco147I AGGCCT 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 45, 544
Eco47I GGWCC 4 cut(s) 79, 512, 791, 821
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 177, 569
Eco88I CYCGRG 2 cut(s) 38, 246
EcoICRI GAGCTC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 136, 501
EcoT14I CCWWGG 2 cut(s) 545, 687
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 45, 544
EgeI GGCGCC 1 cut(s) 608
EheI GGCGCC 1 cut(s) 608
ErhI CCWWGG 2 cut(s) 545, 687
FaeI CATG 4 cut(s) 136, 405, 623, 902
FalI AAGNNNNNCTT 1 cut(s) 919
FaqI GGGAC 2 cut(s) 476, 719
FatI CATG 4 cut(s) 132, 401, 619, 898
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 3 cut(s) 363, 489, 933
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 2 cut(s) 45, 544
Fsp4HI GCNGC 3 cut(s) 363, 489, 933
FspBI CTAG 4 cut(s) 6, 116, 414, 546
GlaI GCGC 1 cut(s) 608
GluI GCNGC 3 cut(s) 363, 489, 933
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 3 cut(s) 106, 226, 549
HaeII RGCGCY 1 cut(s) 610
HaeIII GGCC 1 cut(s) 244
HapII CCGG 3 cut(s) 460, 560, 816
HgaI GACGC 1 cut(s) 458
HhaI GCGC 1 cut(s) 609
Hin1I GRCGYC 2 cut(s) 450, 607
Hin1II CATG 4 cut(s) 136, 405, 623, 902
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 1 cut(s) 770
HpaII CCGG 3 cut(s) 460, 560, 816
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 5 cut(s) 79, 188, 407, 479, 802
Hpy188I TCNGA 3 cut(s) 196, 351, 391
Hpy188III TCNNGA 7 cut(s) 38, 85, 248, 421, 566, 665, 728
Hpy8I GTNNAC 5 cut(s) 79, 188, 407, 479, 802
HpyAV CCTTC 4 cut(s) 56, 201, 420, 597
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 8 cut(s) 92, 99, 230, 329, 365, 401, 623, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 241, 250, 362, 569
HpyF3I CTNAG 1 cut(s) 887
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 2 cut(s) 450, 607
Hsp92II CATG 4 cut(s) 136, 405, 623, 902
HspAI GCGC 1 cut(s) 607
KasI GGCGCC 1 cut(s) 606
KroI GCCGGC 1 cut(s) 559
KroNI GCCGGC 1 cut(s) 561
Kzo9I GATC 5 cut(s) 263, 334, 497, 692, 709
LmnI GCTCC 6 cut(s) 48, 154, 250, 547, 568, 580
Lsp1109I GCAGC 2 cut(s) 349, 475
MaeI CTAG 4 cut(s) 6, 116, 414, 546
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 16, 111
MalI GATC 5 cut(s) 265, 336, 499, 694, 711
MboI GATC 5 cut(s) 263, 334, 497, 692, 709
MboII GAAGA 4 cut(s) 273, 314, 687, 844
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 3 cut(s) 263, 497, 692
MhlI GDGCHC 2 cut(s) 45, 544
MluCI AATT 3 cut(s) 176, 330, 612
Mly113I GGCGCC 1 cut(s) 607
MmeI TCCRAC 1 cut(s) 458
Mph1103I ATGCAT 1 cut(s) 403
MroNI GCCGGC 1 cut(s) 559
MroXI GAANNNNTTC 2 cut(s) 321, 648
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 3 cut(s) 624, 666, 903
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 3 cut(s) 460, 560, 816
MspR9I CCNGG 3 cut(s) 138, 461, 503
MunI CAATTG 1 cut(s) 330
MvaI CCWGG 2 cut(s) 138, 503
MwoI GCNNNNNNNGC 5 cut(s) 133, 241, 250, 362, 569
NaeI GCCGGC 1 cut(s) 561
NarI GGCGCC 1 cut(s) 607
NciI CCSGG 1 cut(s) 461
NdeII GATC 5 cut(s) 263, 334, 497, 692, 709
NgoMIV GCCGGC 1 cut(s) 559
NlaIII CATG 4 cut(s) 136, 405, 623, 902
NlaIV GGNNCC 4 cut(s) 252, 543, 564, 608
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 2 cut(s) 405, 623
PaeI GCATGC 1 cut(s) 623
PceI AGGCCT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PdiI GCCGGC 1 cut(s) 561
PdmI GAANNNNTTC 2 cut(s) 321, 648
PfeI GAWTC 1 cut(s) 770
PkrI GCNGC 3 cut(s) 364, 490, 934
PluTI GGCGCC 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 2 cut(s) 136, 501
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 136, 501
PspN4I GGNNCC 4 cut(s) 252, 543, 564, 608
PspPI GGNCC 4 cut(s) 79, 512, 791, 821
PsuI RGATCY 3 cut(s) 263, 497, 692
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 2 cut(s) 145, 745
RsaNI GTAC 2 cut(s) 144, 744
RseI CAYNNNNRTG 3 cut(s) 624, 666, 903
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 3 cut(s) 363, 489, 933
Sau3AI GATC 5 cut(s) 263, 334, 497, 692, 709
Sau96I GGNCC 4 cut(s) 79, 512, 791, 821
ScrFI CCNGG 3 cut(s) 138, 461, 503
SduI GDGCHC 2 cut(s) 45, 544
SfcI CTRYAG 1 cut(s) 776
SfoI GGCGCC 1 cut(s) 608
SinI GGWCC 4 cut(s) 79, 512, 791, 821
SmiMI CAYNNNNRTG 3 cut(s) 624, 666, 903
SpeI ACTAGT 1 cut(s) 5
SphI GCATGC 1 cut(s) 623
Sse9I AATT 3 cut(s) 176, 330, 612
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 3 cut(s) 107, 578, 932
SspDI GGCGCC 1 cut(s) 606
SspI AATATT 1 cut(s) 595
SspMI CTAG 4 cut(s) 6, 116, 414, 546
SstI GAGCTC 1 cut(s) 45
StuI AGGCCT 1 cut(s) 244
StyD4I CCNGG 3 cut(s) 136, 459, 501
StyI CCWWGG 2 cut(s) 545, 687
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 349
TaqI TCGA 2 cut(s) 31, 315
TasI AATT 3 cut(s) 176, 330, 612
TatI WGTACW 1 cut(s) 743
TauI GCSGC 1 cut(s) 935
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TseI GCWGC 2 cut(s) 362, 488
TspDTI ATGAA 3 cut(s) 213, 291, 483
TspGWI ACGGA 2 cut(s) 783, 844
VpaK11BI GGWCC 4 cut(s) 79, 512, 791, 821
XceI RCATGY 2 cut(s) 405, 623
XmaJI CCTAGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 478
XmnI GAANNNNTTC 2 cut(s) 321, 648
XspI CTAG 4 cut(s) 6, 116, 414, 546
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.