pycom05g05870

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003997
Physical Location & Seq
Forward (+)
7389362 .. 7392634
3273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g05870.1

Sequence Viewer

Length: 621 bp
ATGCCGTCTCGGAGAAGAACGCTCTTAAAGGTCATCATCCTCGGCGACAGCGGGGTGGGGAAGACTTCTTTGATGAATCAATATGTAAACAAGAAGTTTAGCAATCAGTACAAGGCGACAATTGGAGCTGATTTTTTGACAAAAGAAGTGCAGTTTGAAGACAGGCTCTTCACCTTACAGATCTGGGATACAGCTGGCCAAGAACGATTCCAAAGCCTTGGTGTAGCTTTTTACCGTGGTGCTGATTGCTGTGTTCTTGTGTATGATGTGAATTCAATGAAGTCATTTGATAACCTTAACAACTGGAGGGAAGAATTTCTCATTCAGGCAAGTCCTTCAGATCCAGAAAACTTCCCATTTGTCGTTATTGGAAACAAGATTGATGTAGATGGTGGAAATAGTCGAGTGGTTTCAGAGAAAAAGGCTCGAGCTTGGTGTGCATCAAAGGGAAATATTCCATACTATGAAACCTCTGCAAAGGAAGGCATTAATGTGGAAGAAGCTTTCCAGTGCATAGCAAAGGATGCTCTGAAGAGTGGGGAAGAGGAAGAAATATACTTGCCGGATACCATTGATGTTGGAAGCAGCAATCAGCCAAGGTCAACCGGATGCGAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

207

Amino Acids

23.07

Weight (kDa)

4.98

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 174 6.6e-13 ADP-ribosylation factor family
Ras PF00071 10 - 176 1.7e-56 Ras family
Roc PF08477 10 - 128 1.5e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 106 2.2e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 10 - 129 6e-06 Gtr1/RagA G protein conserved region
GTP_EFTU PF00009 46 - 173 2e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 51
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 2 cut(s) 271, 314
AcuI CTGAAG 2 cut(s) 321, 551
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 2 cut(s) 158, 276
AluBI AGCT 5 cut(s) 128, 194, 227, 431, 503
AluI AGCT 5 cut(s) 128, 194, 227, 431, 503
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 585
ApoI RAATTY 2 cut(s) 271, 314
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 426
BalI TGGCCA 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 68, 165
BbvI GCAGC 1 cut(s) 597
BccI CCATC 1 cut(s) 383
BciVI GTATCC 2 cut(s) 181, 559
BcoDI GTCTC 1 cut(s) 12
BfuI GTATCC 2 cut(s) 181, 559
BglII AGATCT 1 cut(s) 180
BisI GCNGC 1 cut(s) 586
BlsI GCNGC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 426
BmsI GCATC 3 cut(s) 449, 514, 599
BpiI GAAGAC 2 cut(s) 68, 165
BpmI CTGGAG 1 cut(s) 325
BsaJI CCNNGG 4 cut(s) 40, 217, 235, 596
BsaWI WCCGGW 1 cut(s) 605
Bse1I ACTGG 2 cut(s) 308, 508
BseDI CCNNGG 4 cut(s) 40, 217, 235, 596
BseGI GGATG 3 cut(s) 36, 529, 614
BseNI ACTGG 2 cut(s) 308, 508
BseXI GCAGC 1 cut(s) 597
BsgI GTGCAG 1 cut(s) 170
BshFI GGCC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 2 cut(s) 563, 606
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 426
Bsp143I GATC 2 cut(s) 180, 340
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 198
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 1 cut(s) 173
BsrI ACTGG 2 cut(s) 308, 508
BssECI CCNNGG 4 cut(s) 40, 217, 235, 596
BssMI GATC 2 cut(s) 180, 340
BssT1I CCWWGG 2 cut(s) 217, 596
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 3 cut(s) 173, 527, 537
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 196
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 36, 529, 614
BstKTI GATC 2 cut(s) 183, 343
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 180, 340
BstMWI GCNNNNNNNGC 2 cut(s) 437, 524
BstV1I GCAGC 1 cut(s) 597
BstV2I GAAGAC 2 cut(s) 68, 165
BstX2I RGATCY 2 cut(s) 180, 340
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 2 cut(s) 180, 340
BsuI GTATCC 2 cut(s) 181, 559
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 3 cut(s) 36, 529, 614
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 109
CviQI GTAC 1 cut(s) 109
DpnI GATC 2 cut(s) 182, 342
DpnII GATC 2 cut(s) 180, 340
EaeI YGGCCR 1 cut(s) 196
Eam1104I CTCTTC 3 cut(s) 173, 527, 537
EarI CTCTTC 3 cut(s) 173, 527, 537
Eco130I CCWWGG 2 cut(s) 217, 596
Eco57I CTGAAG 2 cut(s) 321, 551
Eco88I CYCGRG 1 cut(s) 426
EcoRI GAATTC 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 217, 596
ErhI CCWWGG 2 cut(s) 217, 596
Esp3I CGTCTC 1 cut(s) 12
FaiI YATR 6 cut(s) 84, 264, 460, 465, 515, 556
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 586
FokI GGATG 2 cut(s) 23, 536
Fsp4HI GCNGC 1 cut(s) 586
GluI GCNGC 1 cut(s) 586
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 198
HapII CCGG 2 cut(s) 563, 606
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 2 cut(s) 76, 207
HpaII CCGG 2 cut(s) 563, 606
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 2 cut(s) 88, 603
Hpy188I TCNGA 4 cut(s) 12, 340, 415, 531
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 2 cut(s) 88, 603
HpyAV CCTTC 2 cut(s) 345, 476
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4V TGCA 4 cut(s) 151, 440, 476, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 524
Kzo9I GATC 2 cut(s) 180, 340
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 8 cut(s) 148, 169, 180, 289, 311, 357, 521, 576
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 3 cut(s) 449, 514, 599
MalI GATC 2 cut(s) 182, 342
MboI GATC 2 cut(s) 180, 340
MboII GAAGA 9 cut(s) 27, 73, 160, 170, 323, 509, 544, 554, 560
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 180, 340
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 3 cut(s) 120, 271, 314
MluNI TGGCCA 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 4 cut(s) 50, 300, 481, 538
Mox20I TGGCCA 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 198
MseI TTAA 3 cut(s) 26, 297, 489
MslI CAYNNNNRTG 1 cut(s) 491
Msp20I TGGCCA 1 cut(s) 198
MspA1I CMGCKG 2 cut(s) 51, 194
MspI CCGG 2 cut(s) 563, 606
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 620
MwoI GCNNNNNNNGC 2 cut(s) 437, 524
NdeII GATC 2 cut(s) 180, 340
NmeAIII GCCGAG 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 173
PctI GAATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 76, 207
PkrI GCNGC 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 489
PspXI VCTCGAGB 1 cut(s) 426
PsuI RGATCY 2 cut(s) 180, 340
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 3 cut(s) 26, 297, 489
SatI GCNGC 1 cut(s) 586
Sau3AI GATC 2 cut(s) 180, 340
SfaNI GCATC 3 cut(s) 449, 514, 599
Sfr274I CTCGAG 1 cut(s) 426
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 3 cut(s) 120, 271, 314
SsiI CCGC 1 cut(s) 51
SspI AATATT 1 cut(s) 454
StyI CCWWGG 2 cut(s) 217, 596
TaaI ACNGT 1 cut(s) 236
TaqI TCGA 2 cut(s) 403, 427
TasI AATT 3 cut(s) 120, 271, 314
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 2 cut(s) 76, 207
Tru1I TTAA 3 cut(s) 26, 297, 489
Tru9I TTAA 3 cut(s) 26, 297, 489
TscAI CASTG 1 cut(s) 515
TseI GCWGC 1 cut(s) 585
TspDTI ATGAA 3 cut(s) 89, 293, 480
TspRI CASTG 1 cut(s) 515
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 2 cut(s) 271, 314
XhoI CTCGAG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.