Rroxscaffold_176G00431370

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0003997
Physical Location & Seq
Reverse (-)
998962 .. 1002594
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431370.1

Sequence Viewer

Length: 642 bp
ATGCCGTCTCGGAGAAGAACGCTTCTCAAGGTCATCATCCTCGGCGACAGCGGGGTTGGGAAGACTTCTTTGATGAATCAGTATCCTTTGGAAGCTCTTAAATATGTTAATAAGAAGTTCAGCAATCAATACAAGGCGACTATTGGAGCCGATTTCTTGACGAAAGAAGTGCAGTTCGAAGATAGGCTCTTCACCTTACAGATCTGGGATACCGCTGGTCAAGAACGGTTTCAGAGCCTTGGTGTAGCATTTTATCGTGGTGCTGATTGCTGTGTTCTTGTTTATGACGTTAATTCGATGAAATCTTTTGATAACCTTAACAACTGGAAGGAAGAATTCCTTATTCAGGCTAGTCCTAATGACCCAGAAAACTTTCCATTTGTTGTTATTGGAAACAAGATTGATGTAGATGGTGGAAATAGTAGAGTGGTGTCGGAGAAAAAGGCTCGAGCTTGGTGTGCATCAAAAGGAGACATTCCATACTATGAAACGTCTGCCAAGGAAGGCATTAATGTGGAAGAAGCTTTCCAGTGCATAGCAAGGGATGCTCTGAAGAGTGGAGAAGAGGAGGAGATATACTTGCCGGATACCATTGATGTTGGAAGCAGCAGTCAGCCAAGGTCGAGTGGATGCGAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

213

Amino Acids

23.87

Weight (kDa)

4.92

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 180 2.6e-10 ADP-ribosylation factor family
GTP_EFTU PF00009 9 - 179 3.8e-07 Elongation factor Tu GTP binding domain
Ras PF00071 10 - 184 9.8e-53 Ras family
Roc PF08477 10 - 135 6.8e-30 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 51, 213
AcsI RAATTY 1 cut(s) 335
AcuI CTGAAG 1 cut(s) 572
AfiI CCNNNNNNNGG 1 cut(s) 346
AluBI AGCT 3 cut(s) 95, 452, 524
AluI AGCT 3 cut(s) 95, 452, 524
Alw26I GTCTC 2 cut(s) 12, 465
Ama87I CYCGRG 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 606
ApoI RAATTY 1 cut(s) 335
AseI ATTAAT 1 cut(s) 510
Asp700I GAANNNNTTC 2 cut(s) 228, 372
AsuHPI GGTGA 1 cut(s) 184
AsuII TTCGAA 1 cut(s) 177
AvaI CYCGRG 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 618
BccI CCATC 1 cut(s) 404
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciVI GTATCC 3 cut(s) 93, 202, 580
BcoDI GTCTC 2 cut(s) 12, 465
BfaI CTAG 1 cut(s) 351
BfuI GTATCC 3 cut(s) 93, 202, 580
BglII AGATCT 1 cut(s) 201
BisI GCNGC 1 cut(s) 607
BlsI GCNGC 1 cut(s) 608
BmeT110I CYCGRG 1 cut(s) 447
BmiI GGNNCC 1 cut(s) 148
BmsI GCATC 3 cut(s) 470, 535, 620
BpiI GAAGAC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 40, 238, 498, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 329, 529
BseDI CCNNGG 4 cut(s) 40, 238, 498, 617
BseGI GGATG 3 cut(s) 36, 550, 635
BseLI CCNNNNNNNGG 1 cut(s) 346
BseNI ACTGG 2 cut(s) 329, 529
BseRI GAGGAG 2 cut(s) 581, 584
BseXI GCAGC 1 cut(s) 618
BsgI GTGCAG 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 1 cut(s) 584
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 12, 465
BsmBI CGTCTC 1 cut(s) 12
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 177
Bsp143I GATC 1 cut(s) 201
BspACI CCGC 2 cut(s) 51, 213
BspLI GGNNCC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 194
BspT104I TTCGAA 1 cut(s) 177
BsrI ACTGG 2 cut(s) 329, 529
BssECI CCNNGG 4 cut(s) 40, 238, 498, 617
BssMI GATC 1 cut(s) 201
BssT1I CCWWGG 3 cut(s) 238, 498, 617
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 3 cut(s) 194, 548, 558
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBI TTCGAA 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 3 cut(s) 36, 550, 635
BstKTI GATC 1 cut(s) 204
BstMAI GTCTC 2 cut(s) 12, 465
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 458, 545
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BsuI GTATCC 3 cut(s) 93, 202, 580
BtsCI GGATG 3 cut(s) 36, 550, 635
BtsIMutI CAGTG 1 cut(s) 536
CviJI RGCY 9 cut(s) 95, 149, 187, 237, 350, 446, 452, 524, 616
CviKI_1 RGCY 9 cut(s) 95, 149, 187, 237, 350, 446, 452, 524, 616
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
Eam1104I CTCTTC 3 cut(s) 194, 548, 558
EarI CTCTTC 3 cut(s) 194, 548, 558
Eco130I CCWWGG 3 cut(s) 238, 498, 617
Eco57I CTGAAG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 447
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 335
EcoT14I CCWWGG 3 cut(s) 238, 498, 617
ErhI CCWWGG 3 cut(s) 238, 498, 617
Esp3I CGTCTC 1 cut(s) 12
FaiI YATR 6 cut(s) 105, 285, 481, 486, 536, 577
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 607
FokI GGATG 2 cut(s) 23, 557
Fsp4HI GCNGC 1 cut(s) 607
FspBI CTAG 1 cut(s) 351
GluI GCNGC 1 cut(s) 607
HapII CCGG 1 cut(s) 584
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 584
HphI GGTGA 1 cut(s) 184
Hpy188I TCNGA 4 cut(s) 12, 234, 436, 552
Hpy188III TCNNGA 2 cut(s) 157, 221
HpyAV CCTTC 2 cut(s) 322, 497
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4IV ACGT 2 cut(s) 288, 491
HpyCH4V TGCA 3 cut(s) 172, 461, 534
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 545
HpySE526I ACGT 2 cut(s) 288, 491
Kzo9I GATC 1 cut(s) 201
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 7 cut(s) 190, 201, 310, 332, 378, 542, 597
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 3 cut(s) 470, 535, 620
MaeI CTAG 1 cut(s) 351
MaeII ACGT 2 cut(s) 288, 491
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MboII GAAGA 8 cut(s) 27, 73, 181, 191, 344, 530, 565, 575
MflI RGATCY 1 cut(s) 201
MluCI AATT 2 cut(s) 292, 335
MmeI TCCRAC 2 cut(s) 414, 580
MnlI CCTC 3 cut(s) 50, 559, 562
MroXI GAANNNNTTC 2 cut(s) 228, 372
MseI TTAA 6 cut(s) 99, 108, 291, 318, 510, 640
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 2 cut(s) 51, 215
MspI CCGG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 458, 545
NdeII GATC 1 cut(s) 201
NlaIV GGNNCC 1 cut(s) 148
NmeAIII GCCGAG 1 cut(s) 21
NspV TTCGAA 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 194
PdmI GAANNNNTTC 2 cut(s) 228, 372
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 1 cut(s) 608
PshBI ATTAAT 1 cut(s) 510
PspN4I GGNNCC 1 cut(s) 148
PspXI VCTCGAGB 1 cut(s) 447
PsuI RGATCY 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 512
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 6 cut(s) 99, 108, 291, 318, 510, 640
SatI GCNGC 1 cut(s) 607
Sau3AI GATC 1 cut(s) 201
SetI ASST 9 cut(s) 33, 97, 197, 291, 318, 454, 494, 526, 623
SfaNI GCATC 3 cut(s) 470, 535, 620
Sfr274I CTCGAG 1 cut(s) 447
SfuI TTCGAA 1 cut(s) 177
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 2 cut(s) 26, 447
SmoI CTYRAG 2 cut(s) 26, 447
Sse9I AATT 2 cut(s) 292, 335
SsiI CCGC 2 cut(s) 51, 213
SspMI CTAG 1 cut(s) 351
StyI CCWWGG 3 cut(s) 238, 498, 617
TaaI ACNGT 1 cut(s) 228
TaiI ACGT 2 cut(s) 291, 494
TaqI TCGA 4 cut(s) 177, 296, 448, 623
TasI AATT 2 cut(s) 292, 335
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 6 cut(s) 99, 108, 291, 318, 510, 640
Tru9I TTAA 6 cut(s) 99, 108, 291, 318, 510, 640
TscAI CASTG 1 cut(s) 536
TseI GCWGC 1 cut(s) 606
TspDTI ATGAA 3 cut(s) 89, 314, 501
TspRI CASTG 1 cut(s) 536
VspI ATTAAT 1 cut(s) 510
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 447
XmnI GAANNNNTTC 2 cut(s) 228, 372
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.