RLG00000019749

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003997
Physical Location & Seq
Forward (+)
56286868 .. 56290172
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019749

Sequence Viewer

Length: 624 bp
ATGGCTTCTCGCAGACGCATGCTTCTAAAGGTCATTATCCTCGGCGACAGCGGGGTGGGGAAGACCTCGCTTATGAATCAGTATGTGAATCGTAAGTTTAGTAATCAGTACAAGGCGACAATCGGAGCGGATTTTCTGACCAAGGAGGTTCAGTTTGAAGATAGGTTGTTCACATTGCAGATTTGGGATACTGCTGGGCAAGAAAGGTTTCAAAGTCTTGGTGTGGCTTTCTATCGTGGTGCAGATTGTTGTGTGCTTGTGTATGATGTGAATGTCATGAAATCATTTGATAATCTTAACAACTGGAGGGAAGAATTTCTGATTCAGGCCAGTCCATCCGATCCTGAGAACTTTCCATTCGTTGTGTTGGGGAACAAGATTGATGTTGATGGTGGAAATAGTCGAGTGGTTTCTGAAAAGAAAGCGAAGGCCTGGTGTGCTTCCAAGGGAAACATACCATACTTTGAGACCTCTGCAAAAGAAGGCTTTAACGTGGATGATGCCTTCCAATGTATAGCAAAAAACGCACTGAAGAATGAACCTGAAGAAGAAATATACCTTCCTGACACAATCGATGTTGCTGGTGGAGGGAGGCAGCAAAGATCTACTGGCTGTGAATGTTAA

Protein Analysis

208

Amino Acids

23.23

Weight (kDa)

5.21

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 7 - 174 5.6e-13 ADP-ribosylation factor family
Ras PF00071 10 - 176 7.8e-55 Ras family
Roc PF08477 10 - 128 8.3e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 105 4.1e-07 50S ribosome-binding GTPase
GTP_EFTU PF00009 47 - 174 2.1e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 128
AciI CCGC 2 cut(s) 51, 128
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 551, 564
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 2 cut(s) 158, 212
AjnI CCWGG 1 cut(s) 431
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 1 cut(s) 335
AoxI GGCC 2 cut(s) 327, 429
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 1 cut(s) 314
Asp700I GAANNNNTTC 2 cut(s) 207, 315
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 607
BccI CCATC 2 cut(s) 343, 383
BciT130I CCWGG 1 cut(s) 433
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 461
BfuI GTATCC 1 cut(s) 181
BglII AGATCT 1 cut(s) 602
BisI GCNGC 1 cut(s) 596
BlsI GCNGC 1 cut(s) 597
Bme1390I CCNGG 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 433
BmsI GCATC 1 cut(s) 490
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 325
Bsa29I ATCGAT 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 40, 141, 444
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bse1I ACTGG 3 cut(s) 308, 330, 613
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 3 cut(s) 40, 141, 444
BseGI GGATG 2 cut(s) 335, 502
BseMI GCAATG 1 cut(s) 173
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 3 cut(s) 308, 330, 613
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 194
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 2 cut(s) 329, 431
BshVI ATCGAT 1 cut(s) 573
BsmAI GTCTC 1 cut(s) 461
BsnI GGCC 2 cut(s) 329, 431
Bso31I GGTCTC 1 cut(s) 461
Bsp143I GATC 2 cut(s) 340, 602
BspACI CCGC 2 cut(s) 51, 128
BspANI GGCC 2 cut(s) 329, 431
BspCNI CTCAG 1 cut(s) 337
BspDI ATCGAT 1 cut(s) 573
BspHI TCATGA 1 cut(s) 276
BspPI GGATC 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 173
BsrI ACTGG 3 cut(s) 308, 330, 613
BssECI CCNNGG 3 cut(s) 40, 141, 444
BssMI GATC 2 cut(s) 340, 602
BssT1I CCWWGG 2 cut(s) 141, 444
Bst2UI CCWGG 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 2 cut(s) 335, 502
BstKTI GATC 2 cut(s) 343, 605
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 340, 602
BstMWI GCNNNNNNNGC 2 cut(s) 437, 524
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 329, 431
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 2 cut(s) 335, 502
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 276
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 19, 277
CviJI RGCY 6 cut(s) 5, 227, 329, 431, 486, 612
CviKI_1 RGCY 6 cut(s) 5, 227, 329, 431, 486, 612
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 1 cut(s) 345
DpnI GATC 2 cut(s) 342, 604
DpnII GATC 2 cut(s) 340, 602
Eco130I CCWWGG 2 cut(s) 141, 444
Eco147I AGGCCT 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 461
Eco57I CTGAAG 2 cut(s) 551, 564
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 141, 444
ErhI CCWWGG 2 cut(s) 141, 444
FaeI CATG 2 cut(s) 22, 280
FaiI YATR 9 cut(s) 20, 74, 84, 264, 278, 455, 460, 515, 556
FatI CATG 2 cut(s) 18, 276
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 596
FokI GGATG 2 cut(s) 322, 509
Fsp4HI GCNGC 1 cut(s) 596
GluI GCNGC 1 cut(s) 596
GsaI CCCAGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 2 cut(s) 329, 431
HgaI GACGC 1 cut(s) 24
Hin1II CATG 2 cut(s) 22, 280
HinfI GANTC 3 cut(s) 76, 88, 322
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 5 cut(s) 125, 138, 321, 340, 415
Hpy188III TCNNGA 3 cut(s) 277, 344, 563
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 4 cut(s) 421, 476, 514, 569
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 3 cut(s) 178, 242, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 524
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 2 cut(s) 22, 280
Kzo9I GATC 2 cut(s) 340, 602
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 1 cut(s) 490
MaeII ACGT 1 cut(s) 492
MalI GATC 2 cut(s) 342, 604
MbiI CCGCTC 1 cut(s) 128
MboI GATC 2 cut(s) 340, 602
MboII GAAGA 6 cut(s) 73, 170, 323, 544, 557, 560
MflI RGATCY 1 cut(s) 602
MluCI AATT 1 cut(s) 314
MnlI CCTC 7 cut(s) 50, 76, 139, 300, 481, 581, 585
MroXI GAANNNNTTC 2 cut(s) 207, 315
MseI TTAA 3 cut(s) 297, 489, 622
MspA1I CMGCKG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 433
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 437, 524
NdeII GATC 2 cut(s) 340, 602
NlaIII CATG 2 cut(s) 22, 280
NmeAIII GCCGAG 1 cut(s) 21
NspI RCATGY 1 cut(s) 22
PaeI GCATGC 1 cut(s) 22
PagI TCATGA 1 cut(s) 276
PceI AGGCCT 1 cut(s) 431
PdmI GAANNNNTTC 2 cut(s) 207, 315
PfeI GAWTC 3 cut(s) 76, 88, 322
PkrI GCNGC 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 602
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 297, 489, 622
SatI GCNGC 1 cut(s) 596
Sau3AI GATC 2 cut(s) 340, 602
ScrFI CCNGG 1 cut(s) 433
SetI ASST 9 cut(s) 33, 68, 150, 167, 209, 473, 495, 544, 561
SfaNI GCATC 1 cut(s) 490
SphI GCATGC 1 cut(s) 22
Sse9I AATT 1 cut(s) 314
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 2 cut(s) 51, 128
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 1 cut(s) 431
StyI CCWWGG 2 cut(s) 141, 444
TaiI ACGT 1 cut(s) 495
TaqI TCGA 2 cut(s) 403, 573
TasI AATT 1 cut(s) 314
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 3 cut(s) 76, 88, 322
Tru1I TTAA 3 cut(s) 297, 489, 622
Tru9I TTAA 3 cut(s) 297, 489, 622
TscAI CASTG 1 cut(s) 534
TseI GCWGC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 89, 293, 552
TspRI CASTG 1 cut(s) 534
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 22
XmnI GAANNNNTTC 2 cut(s) 207, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.