Rroxscaffold_7G00203650

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003997
Physical Location & Seq
Forward (+)
52564066 .. 52567667
3602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00203650.1

Sequence Viewer

Length: 621 bp
ATGCCGTCTCGGAGAAGAACGCTCCTCAAGGTCATCATCCTTGGCGACAGCGGGGTTGGGAAGACTTCTTTGATGAATCAATATGTTAATAAGAAGTTCAGCAATCAATACAAGGCGACTATTGGAGCCGATTTCTTGACGAAAGAAGTGCAGTTCGAAGATAGGCTCTTCACCTTACAGATCTGGGATACTGCTGGTCAAGAACGGTTTCAGAGCCTTGGTGTAGCATTTTATCGTGGTGCTGATTGCTGTGTTCTTGTTTATGACGTTAATTCGATGAAATCTTTTGATAACCTTAACAACTGGAAGGAAGAATTCCTTATTCAGGCTAGTCCTAACGACCCAGAAAACTTTCCATTTGTTGTTATTGGAAACAAGATTGATGTAGATGGTGGAAATAGTAGAGTGGTGTCGGAGAAAAAGGCTCGAGCTTGGTGTGCATCAAAAGGGGACATTCCATACTATGAAACGTCTGCCAAGGAAGGCATTAATGTGGAAGAAGCTTTCCAGTGCATAGCAAGAGATGCTCTGAAGAGTGGAGAAGAGGAGGAGATATACTTGCCGGATACCATTGATGTTGGAAGCAGCAGTCAGCCAAGGTCGAGTGGATGCGAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

206

Amino Acids

23.06

Weight (kDa)

4.9

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 173 6.2e-13 ADP-ribosylation factor family
Ras PF00071 10 - 177 2.3e-56 Ras family
Roc PF08477 10 - 128 9e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 106 1.8e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 10 - 129 3.3e-06 Gtr1/RagA G protein conserved region
GTP_EFTU PF00009 46 - 172 1.5e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 51
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 325
AluBI AGCT 2 cut(s) 431, 503
AluI AGCT 2 cut(s) 431, 503
Alw26I GTCTC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 426
ApeKI GCWGC 1 cut(s) 585
ApoI RAATTY 1 cut(s) 314
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 2 cut(s) 207, 351
AsuHPI GGTGA 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 156
AvaI CYCGRG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 597
BccI CCATC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciVI GTATCC 2 cut(s) 181, 559
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 330
BfuI GTATCC 2 cut(s) 181, 559
BglII AGATCT 1 cut(s) 180
BisI GCNGC 1 cut(s) 586
BlsI GCNGC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 426
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 3 cut(s) 449, 514, 599
BpiI GAAGAC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 40, 217, 477, 596
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 308, 508
BseDI CCNNGG 4 cut(s) 40, 217, 477, 596
BseGI GGATG 2 cut(s) 36, 614
BseLI CCNNNNNNNGG 1 cut(s) 325
BseNI ACTGG 2 cut(s) 308, 508
BseRI GAGGAG 3 cut(s) 14, 560, 563
BseXI GCAGC 1 cut(s) 597
BsgI GTGCAG 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 1 cut(s) 563
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 173
BspT104I TTCGAA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 308, 508
BssECI CCNNGG 4 cut(s) 40, 217, 477, 596
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 4 cut(s) 40, 217, 477, 596
Bst4CI ACNGT 1 cut(s) 207
Bst6I CTCTTC 3 cut(s) 173, 527, 537
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 2 cut(s) 36, 614
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 437, 524
BstV1I GCAGC 1 cut(s) 597
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 2 cut(s) 181, 559
BtsCI GGATG 2 cut(s) 36, 614
BtsIMutI CAGTG 1 cut(s) 515
CviJI RGCY 8 cut(s) 128, 166, 216, 329, 425, 431, 503, 595
CviKI_1 RGCY 8 cut(s) 128, 166, 216, 329, 425, 431, 503, 595
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 3 cut(s) 173, 527, 537
EarI CTCTTC 3 cut(s) 173, 527, 537
Eco130I CCWWGG 4 cut(s) 40, 217, 477, 596
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 314
EcoT14I CCWWGG 4 cut(s) 40, 217, 477, 596
ErhI CCWWGG 4 cut(s) 40, 217, 477, 596
Esp3I CGTCTC 1 cut(s) 12
FaiI YATR 6 cut(s) 84, 264, 460, 465, 515, 556
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FaqI GGGAC 1 cut(s) 464
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 586
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 586
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 586
HapII CCGG 1 cut(s) 563
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 563
HphI GGTGA 1 cut(s) 163
Hpy188I TCNGA 4 cut(s) 12, 213, 415, 531
Hpy188III TCNNGA 2 cut(s) 136, 200
HpyAV CCTTC 2 cut(s) 301, 476
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 2 cut(s) 267, 470
HpyCH4V TGCA 3 cut(s) 151, 440, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 524
HpySE526I ACGT 2 cut(s) 267, 470
Kzo9I GATC 1 cut(s) 180
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 2 cut(s) 27, 125
LpnPI CCDG 7 cut(s) 169, 180, 289, 311, 357, 521, 576
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 3 cut(s) 449, 514, 599
MaeI CTAG 1 cut(s) 330
MaeII ACGT 2 cut(s) 267, 470
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 8 cut(s) 27, 73, 160, 170, 323, 509, 544, 554
MflI RGATCY 1 cut(s) 180
MluCI AATT 2 cut(s) 271, 314
MmeI TCCRAC 2 cut(s) 393, 559
MnlI CCTC 3 cut(s) 35, 538, 541
MroXI GAANNNNTTC 2 cut(s) 207, 351
MseI TTAA 5 cut(s) 87, 270, 297, 489, 619
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 437, 524
NdeII GATC 1 cut(s) 180
NlaIV GGNNCC 1 cut(s) 127
NspV TTCGAA 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 173
PdmI GAANNNNTTC 2 cut(s) 207, 351
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 426
PsuI RGATCY 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 5 cut(s) 87, 270, 297, 489, 619
SatI GCNGC 1 cut(s) 586
Sau3AI GATC 1 cut(s) 180
SetI ASST 8 cut(s) 33, 176, 270, 297, 433, 473, 505, 602
SfaNI GCATC 3 cut(s) 449, 514, 599
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 156
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 26, 426
SmoI CTYRAG 2 cut(s) 26, 426
Sse9I AATT 2 cut(s) 271, 314
SsiI CCGC 1 cut(s) 51
SspMI CTAG 1 cut(s) 330
StyI CCWWGG 4 cut(s) 40, 217, 477, 596
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 2 cut(s) 270, 473
TaqI TCGA 4 cut(s) 156, 275, 427, 602
TasI AATT 2 cut(s) 271, 314
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 5 cut(s) 87, 270, 297, 489, 619
Tru9I TTAA 5 cut(s) 87, 270, 297, 489, 619
TscAI CASTG 1 cut(s) 515
TseI GCWGC 1 cut(s) 585
TspDTI ATGAA 3 cut(s) 89, 293, 480
TspRI CASTG 1 cut(s) 515
VspI ATTAAT 1 cut(s) 489
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 207, 351
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.