RLG00000014297

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003997
Physical Location & Seq
Forward (+)
50884742 .. 50887927
3186 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014297

Sequence Viewer

Length: 558 bp
ATGCCGTCTCGGAGAAGAACGCTCCTCAAGGTCATCATCCTCGGCGACAGCGGGGTTGGGAAGACTTCTTTGATGAATCAATATGTTAATAAGAAGTTCAGCAATCAATACAAGGCGACTATTGGAGCCGATTTCTTGACGAAAGAAGTGCAGTTCGAAGATAGGCTCTTCACCTTACAGATCTGGGATACTGCTGGTCAAGAACGGTTTCAGAGCCTTGGTGTAGCATTTTATCGCGGTGCTGATTGCTGTGTTCTTGTTTATGACGTTAATTCGATGAAATCTTTTGATAACCTTAACAACTGGAAGGAAGAATTCCTTATTCAGGCTAGTCCTAACGACCCAGAAAACTTTCCATTTGTTGTTATTGGAAACAAGATTGATGTAGATGGTGGAAATAGTAGAGTGGTGTCGGAGAAAAAGGCTCGAGCTTGGTGTGCATCAAAAGGAGACATTCCGTACTATGAAACGTCTGCCAAGGAAGGCATTAATGTGGAAGAAGCTTTCCAGTGCATAGCAAGGGATGCTCTGAAGAGTGGAGAAGAGGAGGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

186

Amino Acids

20.89

Weight (kDa)

5.07

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 173 4.2e-13 ADP-ribosylation factor family
Ras PF00071 10 - 177 1.5e-56 Ras family
Roc PF08477 10 - 128 6.6e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 106 1.3e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 10 - 129 2.4e-06 Gtr1/RagA G protein conserved region
GTP_EFTU PF00009 45 - 172 1.1e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 237
AciI CCGC 2 cut(s) 51, 237
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 325
AluBI AGCT 2 cut(s) 431, 503
AluI AGCT 2 cut(s) 431, 503
Alw26I GTCTC 2 cut(s) 12, 444
Ama87I CYCGRG 1 cut(s) 426
ApoI RAATTY 1 cut(s) 314
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 2 cut(s) 207, 351
AsuHPI GGTGA 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 156
AvaI CYCGRG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 68
BccI CCATC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 12, 444
BfaI CTAG 1 cut(s) 330
BfuI GTATCC 1 cut(s) 181
BglII AGATCT 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 426
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 2 cut(s) 449, 514
BpiI GAAGAC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 11
BsaJI CCNNGG 3 cut(s) 40, 217, 477
BsaXI ACNNNNNCTCC 1 cut(s) 539
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 308, 508
BseDI CCNNGG 3 cut(s) 40, 217, 477
BseGI GGATG 2 cut(s) 36, 529
BseLI CCNNNNNNNGG 1 cut(s) 325
BseNI ACTGG 2 cut(s) 308, 508
BseRI GAGGAG 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 2 cut(s) 12, 444
BsmBI CGTCTC 1 cut(s) 12
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 2 cut(s) 51, 237
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 173
BspT104I TTCGAA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 308, 508
BssECI CCNNGG 3 cut(s) 40, 217, 477
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 217, 477
Bst4CI ACNGT 1 cut(s) 207
Bst6I CTCTTC 3 cut(s) 173, 527, 537
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 2 cut(s) 36, 529
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 2 cut(s) 12, 444
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 437, 524
BstUI CGCG 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 36, 529
BtsIMutI CAGTG 1 cut(s) 515
Csp6I GTAC 1 cut(s) 460
CviJI RGCY 7 cut(s) 128, 166, 216, 329, 425, 431, 503
CviKI_1 RGCY 7 cut(s) 128, 166, 216, 329, 425, 431, 503
CviQI GTAC 1 cut(s) 460
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 3 cut(s) 173, 527, 537
EarI CTCTTC 3 cut(s) 173, 527, 537
Eco130I CCWWGG 2 cut(s) 217, 477
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 217, 477
ErhI CCWWGG 2 cut(s) 217, 477
Esp3I CGTCTC 1 cut(s) 12
FaiI YATR 4 cut(s) 84, 264, 465, 515
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FauI CCCGC 1 cut(s) 44
FokI GGATG 2 cut(s) 23, 536
FspBI CTAG 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 1 cut(s) 76
HphI GGTGA 1 cut(s) 163
Hpy188I TCNGA 4 cut(s) 12, 213, 415, 531
Hpy188III TCNNGA 2 cut(s) 136, 200
HpyAV CCTTC 2 cut(s) 301, 476
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 2 cut(s) 267, 470
HpyCH4V TGCA 3 cut(s) 151, 440, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 524
HpySE526I ACGT 2 cut(s) 267, 470
Kzo9I GATC 1 cut(s) 180
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 2 cut(s) 27, 125
LpnPI CCDG 6 cut(s) 169, 180, 289, 311, 357, 521
LweI GCATC 2 cut(s) 449, 514
MaeI CTAG 1 cut(s) 330
MaeII ACGT 2 cut(s) 267, 470
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 8 cut(s) 27, 73, 160, 170, 323, 509, 544, 554
MflI RGATCY 1 cut(s) 180
MluCI AATT 2 cut(s) 271, 314
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 4 cut(s) 35, 50, 538, 541
MroXI GAANNNNTTC 2 cut(s) 207, 351
MseI TTAA 4 cut(s) 87, 270, 297, 489
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 51
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 437, 524
NdeII GATC 1 cut(s) 180
NlaIV GGNNCC 1 cut(s) 127
NmeAIII GCCGAG 1 cut(s) 21
NspV TTCGAA 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 173
PdmI GAANNNNTTC 2 cut(s) 207, 351
PfeI GAWTC 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 426
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 4 cut(s) 87, 270, 297, 489
Sau3AI GATC 1 cut(s) 180
SetI ASST 7 cut(s) 33, 176, 270, 297, 433, 473, 505
SfaNI GCATC 2 cut(s) 449, 514
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 156
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 26, 426
SmoI CTYRAG 2 cut(s) 26, 426
Sse9I AATT 2 cut(s) 271, 314
SsiI CCGC 2 cut(s) 51, 237
SspMI CTAG 1 cut(s) 330
StyI CCWWGG 2 cut(s) 217, 477
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 2 cut(s) 270, 473
TaqI TCGA 3 cut(s) 156, 275, 427
TasI AATT 2 cut(s) 271, 314
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 4 cut(s) 87, 270, 297, 489
Tru9I TTAA 4 cut(s) 87, 270, 297, 489
TscAI CASTG 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 89, 293, 480
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 1 cut(s) 515
VspI ATTAAT 1 cut(s) 489
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 207, 351
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.