Rorug06G0007200

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003997
Physical Location & Seq
Forward (+)
1040032 .. 1064330
24299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0007200.1

Sequence Viewer

Length: 3312 bp
ATGGGGCAACAAGTCATGCCAGTGTTCTACCAAGTGGATCCATCTCAAGTGCGGAAGAACAAGGGATATTTTTTGCAGGCCTTTACAGAACACGAAGAAGTTCATTGGGAGAACAGGGATAAAGTAAGGAAGTGGAGGGCTGCTTTGACAGGCGTGGCCAATCTTTCTGGGTGGGATTTGAGGAACATGGATGACGGAAAATTGATTGAGGACATTGTTACGTATATACTTCGGAGACTAGGTCATACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGTATAGGTAAGACAACACTTGCTCGAGCCATCCATGATCGAATTTGTCAGGATTTTGACCAAAGCTGCATTCTTTCCAATGTTAGAGAAAAATCTGAAAAGGATGGCCTAGTTTCTCTTCAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTATTATGATAGAAAGGCGGTTATGTAAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCGGGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGATGTTGATGATACATACAAGGCTACTGGGTTAGCTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAATAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATCTGGTAGAAGCACCAATGAATGGGAAAGTACAATAGAGAGGCTACATAATACACCAAATAGAAAAGTTATGGGTGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAAGGTTCTTGAGGAAAAATCTCTCATTACTATTTTTAAGAACAAGCTGTCGATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTATCCTGAAGTTATCCACAATGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGAGTTGCCTGAGTTAGAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAACGTGGATCTTCCCAAAGGCCTCACTTTTCTGTCTAATTCCTTGAGACTCCTTGAATGGAGTGGGTACCCCTTAAGATCCCTCCCACAGAATTTTGAACCAGACGAACTTTTTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGAAAGGAGCAAAGAATTTTGACAAGTTGAAGTTCATCAAACTATGCCATTCTCAAAAAATAGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCTGTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAGTCTTGAGAATCTGCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTGATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCCGAGTTCGCTGGAAATATGAAGCGTTTATCAAAGCTTTATCTCAATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCACTGAATCTTAGCAACTGCAAAAATCTTGTATGCCTTGCAAGCACCTTAGAACTGCTTAGTAGATTGGAGTCTCTTAAAAAGCTTGATCTTTCTGGATGTTTGAAACTCGGCGAACAGGATAGTGTGAGGGAGAATGGTGCATTTAGAACTGCTGTAAATCATCTTACAAAACGTGGTTGTGAAGTTGTTGGGAAATATTTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCTGATATAGAACCAATGAGTATGCAGTTGCCTGCATCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAGTGAGTATCCGGTTGCCACTATCTCGTCTATGTAATTTAACAGATCTAAACATAAGTAACTGCAATCTTGTTGACGCTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAAAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGAAAATGCTGTTTGATGTATCCAATTTCAATCGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCAGCTGTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGCAAGTTTCTAGTGTGACGGAAACTTTTCAAATTGTAATTCCTGGAAGTAGAATTCCTGAGTGGTTTCATCATCGAAGATTTGCGAGTTCATTAACTGTAGACCTACTTCCAGATTGGAATCAGAGTAGGTTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCAGGTTGATGAGCTTGATATCTGTCAGTTCAAGACTTTTAAAGCTACTCATCATCTTGTATGTTGCCTGAAGCTGAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCAGAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACAAGAGGGCCAGGCCTGAGGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGTTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTCCTCCATATGAGGATATATTGACTGATTTTGAGAATCAAGTTGAAGGGGAAACCATTCACCCAAAAAAAAAGTTGGAAGGGGAAACTTGTGGTACTGGTAGTAGAACCTGCACCCTTGAAGAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

1103

Amino Acids

125.45

Weight (kDa)

6.75

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 93 5.2e-17 TIR domain
NB-ARC PF00931 114 - 265 5.3e-15 NB-ARC domain
WHD_ROQ1 PF23282 332 - 403 9e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 503 - 621 2.6e-06 Leucine-rich repeat region
LRR_13 PF23286 592 - 681 6.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 898 - 1046 2.4e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3299
Acc65I GGTACC 1 cut(s) 1458
AccB1I GGYRCC 1 cut(s) 1458
AccB7I CCANNNNNTGG 1 cut(s) 912
AccI GTMKAC 2 cut(s) 2754, 2928
AciI CCGC 5 cut(s) 52, 295, 329, 370, 574
AclWI GGATC 8 cut(s) 32, 45, 352, 365, 624, 1062, 1407, 1464
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 5 cut(s) 414, 1360, 1483, 1558, 2706
AcuI CTGAAG 4 cut(s) 956, 1269, 1289, 2921
AdeI CACNNNGTG 1 cut(s) 1264
AfaI GTAC 7 cut(s) 922, 1037, 1460, 2817, 3028, 3114, 3277
AfiI CCNNNNNNNGG 4 cut(s) 335, 912, 1100, 1303
AflII CTTAAG 1 cut(s) 1465
AjnI CCWGG 5 cut(s) 1093, 1219, 2695, 2970, 3085
AjuI GAANNNNNNNTTGG 4 cut(s) 426, 458, 1083, 1115
Alw21I GWGCWC 1 cut(s) 2829
Alw26I GTCTC 7 cut(s) 229, 1371, 1432, 1637, 1868, 2004, 3186
AlwI GGATC 8 cut(s) 32, 45, 352, 365, 624, 1062, 1407, 1464
AlwNI CAGNNNCTG 1 cut(s) 2409
Ama87I CYCGRG 2 cut(s) 396, 1826
AoxI GGCC 7 cut(s) 78, 156, 478, 1411, 1918, 3083, 3088
ApeKI GCWGC 5 cut(s) 140, 331, 438, 2318, 2610
ApoI RAATTY 5 cut(s) 414, 1360, 1483, 1558, 2706
ArsI GACNNNNNNTTYG 4 cut(s) 1487, 1519, 2306, 2338
AseI ATTAAT 1 cut(s) 2430
Asp700I GAANNNNTTC 7 cut(s) 99, 289, 762, 797, 1194, 2679, 3237
Asp718I GGTACC 1 cut(s) 1458
AspS9I GGNCC 4 cut(s) 665, 2136, 2406, 3083
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 4 cut(s) 767, 2978, 3109, 3233
AvaI CYCGRG 2 cut(s) 396, 1826
AvaII GGWCC 3 cut(s) 665, 2136, 2406
AxyI CCTNAGG 2 cut(s) 518, 3092
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 2 cut(s) 37, 357
BanI GGYRCC 1 cut(s) 1458
BarI GAAGNNNNNNTAC 2 cut(s) 2745, 2777
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 1370
Bbv12I GWGCWC 1 cut(s) 2829
BbvCI CCTCAGC 1 cut(s) 2636
BbvI GCAGC 5 cut(s) 127, 343, 425, 2305, 2622
BccI CCATC 6 cut(s) 49, 410, 470, 980, 2482, 2498
BceAI ACGGC 1 cut(s) 2947
BciT130I CCWGG 5 cut(s) 1095, 1221, 2697, 2972, 3087
BciVI GTATCC 3 cut(s) 1254, 2258, 2548
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 7 cut(s) 229, 1371, 1432, 1637, 1868, 2004, 3186
BfaI CTAG 8 cut(s) 239, 482, 992, 1073, 1088, 2148, 2664, 3140
BfmI CTRYAG 3 cut(s) 2217, 2751, 3308
BfrI CTTAAG 1 cut(s) 1465
BfuAI ACCTGC 1 cut(s) 3299
BfuI GTATCC 3 cut(s) 1254, 2258, 2548
BglI GCCNNNNNGGC 1 cut(s) 1917
BglII AGATCT 2 cut(s) 1651, 2284
BisI GCNGC 5 cut(s) 141, 332, 439, 2319, 2611
BlsI GCNGC 5 cut(s) 142, 333, 440, 2320, 2612
Bme1390I CCNGG 6 cut(s) 669, 1095, 1221, 2697, 2972, 3087
Bme18I GGWCC 3 cut(s) 665, 2136, 2406
BmeT110I CYCGRG 2 cut(s) 396, 1826
BmgT120I GGNCC 4 cut(s) 665, 2136, 2406, 3083
BmiI GGNNCC 3 cut(s) 39, 359, 1460
BmrFI CCNGG 6 cut(s) 669, 1095, 1221, 2697, 2972, 3087
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 4 cut(s) 1149, 1898, 2207, 2409
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 1370
Bpu10I CCTNAGC 3 cut(s) 710, 849, 2636
BpuEI CTTGAG 6 cut(s) 30, 1136, 1456, 1661, 1766, 3080
BpuMI CCSGG 1 cut(s) 669
Bsa29I ATCGAT 1 cut(s) 2551
BsaAI YACGTR 1 cut(s) 222
BsaBI GATNNNNATC 2 cut(s) 615, 2772
BsaJI CCNNGG 3 cut(s) 361, 668, 1302
BsaWI WCCGGW 1 cut(s) 2250
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 912, 1100, 1303
Bse1I ACTGG 4 cut(s) 20, 662, 745, 3283
Bse21I CCTNAGG 2 cut(s) 518, 3092
Bse8I GATNNNNATC 2 cut(s) 615, 2772
BseBI CCWGG 5 cut(s) 1095, 1221, 2697, 2972, 3087
BseCI ATCGAT 1 cut(s) 2551
BseDI CCNNGG 3 cut(s) 361, 668, 1302
BseJI GATNNNNATC 2 cut(s) 615, 2772
BseLI CCNNNNNNNGG 4 cut(s) 335, 912, 1100, 1303
BseMII CTCAG 9 cut(s) 509, 863, 1311, 1387, 1904, 2357, 2627, 2703, 3083
BseNI ACTGG 4 cut(s) 20, 662, 745, 3283
BseRI GAGGAG 1 cut(s) 3174
BseXI GCAGC 5 cut(s) 127, 343, 425, 2305, 2622
BsgI GTGCAG 1 cut(s) 3277
BshFI GGCC 7 cut(s) 80, 158, 480, 1413, 1920, 3085, 3090
BshNI GGYRCC 1 cut(s) 1458
BshVI ATCGAT 1 cut(s) 2551
BsiHKAI GWGCWC 1 cut(s) 2829
BsiHKCI CYCGRG 2 cut(s) 396, 1826
BsiSI CCGG 2 cut(s) 668, 2251
BslFI GGGAC 3 cut(s) 668, 1192, 2992
BslI CCNNNNNNNGG 4 cut(s) 335, 912, 1100, 1303
BsmAI GTCTC 7 cut(s) 229, 1371, 1432, 1637, 1868, 2004, 3186
BsmFI GGGAC 3 cut(s) 668, 1192, 2992
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 7 cut(s) 80, 158, 480, 1413, 1920, 3085, 3090
BsoBI CYCGRG 2 cut(s) 396, 1826
Bsp1286I GDGCHC 1 cut(s) 2829
Bsp1407I TGTACA 1 cut(s) 1035
Bsp19I CCATGG 2 cut(s) 361, 1302
BspACI CCGC 5 cut(s) 52, 295, 329, 370, 574
BspANI GGCC 7 cut(s) 80, 158, 480, 1413, 1920, 3085, 3090
BspCNI CTCAG 9 cut(s) 510, 862, 1312, 1386, 1905, 2358, 2628, 2704, 3084
BspDI ATCGAT 1 cut(s) 2551
BspLI GGNNCC 3 cut(s) 39, 359, 1460
BspMI ACCTGC 1 cut(s) 3299
BspPI GGATC 8 cut(s) 32, 45, 352, 365, 624, 1062, 1407, 1464
BspT107I GGYRCC 1 cut(s) 1458
BspTI CTTAAG 1 cut(s) 1465
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 4 cut(s) 20, 662, 745, 3283
BssECI CCNNGG 3 cut(s) 361, 668, 1302
BssNAI GTATAC 1 cut(s) 2929
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 2 cut(s) 361, 1302
Bst1107I GTATAC 1 cut(s) 2929
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 5 cut(s) 1095, 1221, 2697, 2972, 3087
Bst4CI ACNGT 3 cut(s) 2752, 2831, 3047
Bst6I CTCTTC 3 cut(s) 262, 495, 2908
BstAFI CTTAAG 1 cut(s) 1465
BstAPI GCANNNNNTGC 2 cut(s) 1168, 3136
BstAUI TGTACA 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 222
BstC8I GCNNGC 6 cut(s) 78, 368, 1085, 1969, 2196, 2943
BstDSI CCRYGG 2 cut(s) 361, 1302
BstMAI GTCTC 7 cut(s) 229, 1371, 1432, 1637, 1868, 2004, 3186
BstMWI GCNNNNNNNGC 7 cut(s) 1159, 1168, 1917, 1968, 2324, 2333, 3136
BstNI CCWGG 5 cut(s) 1095, 1221, 2697, 2972, 3087
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 6 cut(s) 667, 1093, 1219, 2695, 2970, 3085
BstSFI CTRYAG 3 cut(s) 2217, 2751, 3308
BstSNI TACGTA 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 127, 343, 425, 2305, 2622
BstV2I GAAGAC 1 cut(s) 1370
BstX2I RGATCY 6 cut(s) 37, 357, 1399, 1469, 1651, 2284
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 6 cut(s) 37, 357, 1399, 1469, 1651, 2284
BstZ17I GTATAC 1 cut(s) 2929
Bsu15I ATCGAT 1 cut(s) 2551
Bsu36I CCTNAGG 2 cut(s) 518, 3092
BsuI GTATCC 3 cut(s) 1254, 2258, 2548
BsuRI GGCC 7 cut(s) 80, 158, 480, 1413, 1920, 3085, 3090
BsuTUI ATCGAT 1 cut(s) 2551
BtgI CCRYGG 2 cut(s) 361, 1302
BtsIMutI CAGTG 4 cut(s) 27, 1928, 2247, 2516
BveI ACCTGC 1 cut(s) 3299
Cac8I GCNNGC 6 cut(s) 78, 368, 1085, 1969, 2196, 2943
CaiI CAGNNNCTG 1 cut(s) 2409
Cfr13I GGNCC 4 cut(s) 665, 2136, 2406, 3083
ClaI ATCGAT 1 cut(s) 2551
CpoI CGGWCCG 1 cut(s) 665
CseI GACGC 1 cut(s) 2324
Csp6I GTAC 7 cut(s) 921, 1036, 1459, 2816, 3027, 3113, 3276
CspI CGGWCCG 1 cut(s) 665
CviAII CATG 9 cut(s) 16, 187, 362, 367, 407, 1163, 1303, 2133, 2822
CviQI GTAC 7 cut(s) 921, 1036, 1459, 2816, 3027, 3113, 3276
DraI TTTAAA 3 cut(s) 780, 1107, 2872
DraIII CACNNNGTG 1 cut(s) 1264
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 3 cut(s) 262, 495, 2908
EarI CTCTTC 3 cut(s) 262, 495, 2908
Eco105I TACGTA 1 cut(s) 222
Eco130I CCWWGG 2 cut(s) 361, 1302
Eco147I AGGCCT 3 cut(s) 80, 1413, 3090
Eco32I GATATC 1 cut(s) 2851
Eco47I GGWCC 3 cut(s) 665, 2136, 2406
Eco57I CTGAAG 4 cut(s) 956, 1269, 1289, 2921
Eco81I CCTNAGG 2 cut(s) 518, 3092
Eco88I CYCGRG 2 cut(s) 396, 1826
EcoO109I RGGNCCY 1 cut(s) 2406
EcoRI GAATTC 1 cut(s) 2706
EcoRII CCWGG 5 cut(s) 1093, 1219, 2695, 2970, 3085
EcoRV GATATC 1 cut(s) 2851
EcoT14I CCWWGG 2 cut(s) 361, 1302
EcoT22I ATGCAT 1 cut(s) 1164
ErhI CCWWGG 2 cut(s) 361, 1302
FaeI CATG 9 cut(s) 19, 190, 365, 370, 410, 1166, 1306, 2136, 2825
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 3 cut(s) 668, 1192, 2992
FatI CATG 9 cut(s) 15, 186, 361, 366, 406, 1162, 1302, 2132, 2821
FauI CCCGC 2 cut(s) 302, 363
FauNDI CATATG 2 cut(s) 323, 3190
FblI GTMKAC 2 cut(s) 2754, 2928
Fnu4HI GCNGC 5 cut(s) 141, 332, 439, 2319, 2611
Fsp4HI GCNGC 5 cut(s) 141, 332, 439, 2319, 2611
FspBI CTAG 8 cut(s) 239, 482, 992, 1073, 1088, 2148, 2664, 3140
GluI GCNGC 5 cut(s) 141, 332, 439, 2319, 2611
HaeIII GGCC 7 cut(s) 80, 158, 480, 1413, 1920, 3085, 3090
HapII CCGG 2 cut(s) 668, 2251
HgaI GACGC 1 cut(s) 2324
Hin1II CATG 9 cut(s) 19, 190, 365, 370, 410, 1166, 1306, 2136, 2825
HincII GTYRAC 3 cut(s) 268, 532, 2314
HindII GTYRAC 3 cut(s) 268, 532, 2314
HindIII AAGCTT 4 cut(s) 1859, 2009, 2387, 2417
HpaII CCGG 2 cut(s) 668, 2251
HphI GGTGA 4 cut(s) 767, 2978, 3109, 3233
Hpy166II GTNNAC 7 cut(s) 268, 532, 584, 1226, 2314, 2755, 2929
Hpy8I GTNNAC 7 cut(s) 268, 532, 584, 1226, 2314, 2755, 2929
Hpy99I CGWCG 1 cut(s) 1203
HpyAV CCTTC 6 cut(s) 1039, 1652, 2154, 3094, 3221, 3254
HpyCH4III ACNGT 3 cut(s) 2752, 2831, 3047
HpyCH4IV ACGT 4 cut(s) 221, 1395, 2101, 3115
HpyF10VI GCNNNNNNNGC 7 cut(s) 1159, 1168, 1917, 1968, 2324, 2333, 3136
HpySE526I ACGT 4 cut(s) 221, 1395, 2101, 3115
Hsp92II CATG 9 cut(s) 19, 190, 365, 370, 410, 1166, 1306, 2136, 2825
KpnI GGTACC 1 cut(s) 1462
LmnI GCTCC 2 cut(s) 554, 1550
Lsp1109I GCAGC 5 cut(s) 127, 343, 425, 2305, 2622
LweI GCATC 4 cut(s) 1149, 1898, 2207, 2409
MaeI CTAG 8 cut(s) 239, 482, 992, 1073, 1088, 2148, 2664, 3140
MaeII ACGT 4 cut(s) 221, 1395, 2101, 3115
MaeIII GTNAC 7 cut(s) 217, 685, 1060, 1391, 2297, 2615, 2668
MfeI CAATTG 2 cut(s) 1902, 2581
MflI RGATCY 6 cut(s) 37, 357, 1399, 1469, 1651, 2284
MhlI GDGCHC 1 cut(s) 2829
MlsI TGGCCA 1 cut(s) 158
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 5 cut(s) 1214, 1434, 1750, 2006, 2987
MmeI TCCRAC 2 cut(s) 1675, 3237
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 1164
MroXI GAANNNNTTC 7 cut(s) 99, 289, 762, 797, 1194, 2679, 3237
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 3 cut(s) 20, 284, 1307
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 2613
MspCI CTTAAG 1 cut(s) 1465
MspI CCGG 2 cut(s) 668, 2251
MspR9I CCNGG 6 cut(s) 669, 1095, 1221, 2697, 2972, 3087
MunI CAATTG 2 cut(s) 1902, 2581
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 5 cut(s) 1095, 1221, 2697, 2972, 3087
MwoI GCNNNNNNNGC 7 cut(s) 1159, 1168, 1917, 1968, 2324, 2333, 3136
NciI CCSGG 1 cut(s) 669
NcoI CCATGG 2 cut(s) 361, 1302
NdeI CATATG 2 cut(s) 323, 3190
NlaIII CATG 9 cut(s) 19, 190, 365, 370, 410, 1166, 1306, 2136, 2825
NlaIV GGNNCC 3 cut(s) 39, 359, 1460
NmeAIII GCCGAG 1 cut(s) 2016
NmuCI GTSAC 2 cut(s) 1060, 2668
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 1 cut(s) 370
PaeI GCATGC 1 cut(s) 370
PaeR7I CTCGAG 1 cut(s) 396
PceI AGGCCT 3 cut(s) 80, 1413, 3090
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 7 cut(s) 99, 289, 762, 797, 1194, 2679, 3237
PflFI GACNNNGTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 912
PfoI TCCNGGA 1 cut(s) 2695
PkrI GCNGC 5 cut(s) 142, 333, 440, 2320, 2612
PleI GAGTC 5 cut(s) 1213, 1434, 1749, 2005, 2986
PpsI GAGTC 5 cut(s) 1213, 1434, 1749, 2005, 2986
Ppu21I YACGTR 1 cut(s) 222
PpuMI RGGWCCY 1 cut(s) 2406
PshBI ATTAAT 1 cut(s) 2430
Psp5II RGGWCCY 1 cut(s) 2406
Psp6I CCWGG 5 cut(s) 1093, 1219, 2695, 2970, 3085
PspGI CCWGG 5 cut(s) 1093, 1219, 2695, 2970, 3085
PspN4I GGNNCC 3 cut(s) 39, 359, 1460
PspPI GGNCC 4 cut(s) 665, 2136, 2406, 3083
PspPPI RGGWCCY 1 cut(s) 2406
PspXI VCTCGAGB 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 2409
PsuI RGATCY 6 cut(s) 37, 357, 1399, 1469, 1651, 2284
PsyI GACNNNGTC 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 2613
RsaI GTAC 7 cut(s) 922, 1037, 1460, 2817, 3028, 3114, 3277
RsaNI GTAC 7 cut(s) 921, 1036, 1459, 2816, 3027, 3113, 3276
RseI CAYNNNNRTG 3 cut(s) 20, 284, 1307
Rsr2I CGGWCCG 1 cut(s) 665
RsrII CGGWCCG 1 cut(s) 665
SatI GCNGC 5 cut(s) 141, 332, 439, 2319, 2611
Sau96I GGNCC 4 cut(s) 665, 2136, 2406, 3083
SchI GAGTC 5 cut(s) 1214, 1434, 1750, 2006, 2987
ScrFI CCNGG 6 cut(s) 669, 1095, 1221, 2697, 2972, 3087
SduI GDGCHC 1 cut(s) 2829
SfaNI GCATC 4 cut(s) 1149, 1898, 2207, 2409
SfcI CTRYAG 3 cut(s) 2217, 2751, 3308
Sfr274I CTCGAG 1 cut(s) 396
SinI GGWCC 3 cut(s) 665, 2136, 2406
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 3 cut(s) 20, 284, 1307
SmlI CTYRAG 8 cut(s) 45, 396, 1115, 1435, 1465, 1640, 1745, 3059
SmoI CTYRAG 8 cut(s) 45, 396, 1115, 1435, 1465, 1640, 1745, 3059
SnaBI TACGTA 1 cut(s) 222
SphI GCATGC 1 cut(s) 370
SseBI AGGCCT 3 cut(s) 80, 1413, 3090
SsiI CCGC 5 cut(s) 52, 295, 329, 370, 574
SspI AATATT 1 cut(s) 2126
SspMI CTAG 8 cut(s) 239, 482, 992, 1073, 1088, 2148, 2664, 3140
StuI AGGCCT 3 cut(s) 80, 1413, 3090
StyD4I CCNGG 6 cut(s) 667, 1093, 1219, 2695, 2970, 3085
StyI CCWWGG 2 cut(s) 361, 1302
TaaI ACNGT 3 cut(s) 2752, 2831, 3047
TaiI ACGT 4 cut(s) 224, 1398, 2104, 3118
TaqI TCGA 6 cut(s) 397, 412, 1157, 1201, 2551, 2728
TaqII GACCGA 1 cut(s) 653
TatI WGTACW 3 cut(s) 920, 1035, 2815
TscAI CASTG 4 cut(s) 27, 1935, 2247, 2523
TseFI GTSAC 2 cut(s) 1060, 2668
TseI GCWGC 5 cut(s) 140, 331, 438, 2318, 2610
Tsp45I GTSAC 2 cut(s) 1060, 2668
TspGWI ACGGA 3 cut(s) 210, 849, 2687
TspRI CASTG 4 cut(s) 27, 1935, 2247, 2523
Tth111I GACNNNGTC 1 cut(s) 240
Van91I CCANNNNNTGG 1 cut(s) 912
Vha464I CTTAAG 1 cut(s) 1465
VpaK11BI GGWCC 3 cut(s) 665, 2136, 2406
VspI ATTAAT 1 cut(s) 2430
XapI RAATTY 5 cut(s) 414, 1360, 1483, 1558, 2706
XbaI TCTAGA 1 cut(s) 2147
XceI RCATGY 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 396
XmiI GTMKAC 2 cut(s) 2754, 2928
XmnI GAANNNNTTC 7 cut(s) 99, 289, 762, 797, 1194, 2679, 3237
XspI CTAG 8 cut(s) 239, 482, 992, 1073, 1088, 2148, 2664, 3140
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.