pycom05g08410

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009109
Physical Location & Seq
Forward (+)
11023487 .. 11026731
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1917 bp
ATGGAGATCGCCCCGGCCCTGCCGCCGTCCGCTGACCGGGACATCCCAATCGATACCGGTAAGACGTCGTCGTCTCAGGAGAACGAAGCGAGAGCCGCGGCGGCGGCGACGACGACGACCTCGTCTTCGTCGGTGGCGGTCAAGTACGACGATGAGGAAGAGGAGGAGGACGTGTGCCGGATCTGTAGAAACCCGGGCGACGCCGATAACCCGCTCCGGTACCCGTGCGCTTGTAGCGGAAGCATCAAATTTGTCCACCAGGATTGCCTCCTGCAGTGGCTCAATCACAGCAATGCTCGCCAATGCGAGGTTTGCAAGCATGCATTTTCGTTCTCTCCGGTTTATGCTGAGAATGCCCCTGCAAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTCTTTCTGCGCCTTGGTTTTGTGCTTTCTGTTTGGCTCCTCATTATACCTTTTATTACATTTTGGATATGGCGGTTGGCTTTTGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTTAGTCATTTATCCACTACAGTAATCCTTACCGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTTATTTTTCTCGGGGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTAGGAGGACAGGATGCTGACAGAGAAGATGAAGGGGAAAGAAATGGTGCCCGTCCTGCAAGGAGAGCTCCTGGACAAGCTAACAGGAACTTTGTTGGTGATGTGAATGGGGAAGATGCTGCTGGAGCACAAGGGATTGCTGGAGCAGGTCAAATGATTAGAAGGAATGCAGAAAATGTTGCTGCTCGGTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTGGAACAGATGTTTGATGGTCTGGATGATGCTGATGGCGCTGAGGATGTACCGTTTGATGAGCTCGTTGGCATGCAGGGACCTGTATTTCATTTAGTTGAAAATGCATTCACTGTTCTGGCCAGCAATATGATATTCCTTGGCGTAGTAATCTTTGTGCCTTTCTCAATTGGTCGGATAATACTCTATCATTTGTCTTGGCTTTTCTCCACTGCTACTCGTCCAGTATTGTCAACGGTCATGCCACTCACAGAGTCAGCCCTTTCCTTGGCAAATGTGACATTGAAGAATGCATTAACGGCCGTAACAAATTTATCATCTGAAAGCCAACAAAGCGGTATGGTCAGCCAGGTTGAAGAGACCCTGAAAGCAAACATGAGTGGACTAAATGAGGTAGCAAACAATATAAGTTCACCACTTTCAGCAGACTTCCTGAAAGGGGCAACGCTTGGGACATCAAGGCTGTCTGATGTTACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGCATTGTTGCTTTGATTCGGTACACTAGAGGTGAGCCATTGACTTTGGGAAGGTTCTATGGTATTGCTTCTATGGCAGAGACAATTCCATCTCTCTTCAGGCAGTTCTTGGCAGCAATGAGGCATTTGATGACTATGATTAAGGTTGCATTTCTTCTAGTCATTGAACTGGGGGTATTTCCATTGATGTGTGGATGGTGGCTAGATGTTTGCACAATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTTCAGTTCTTCTCAGCATCTCCCTTAGCCAGTTCACTGGTCCATTGGGTTGTTGGAATTGTATACATGCTACAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCGATCTGATCCAAACTACAATCCCTTCCGCGATCTTAATGATCGATCCGGTGCACAAACATGCTCGCAGGGTCCTGTTATCTGTTGCGGTGGTGTCGGACCCATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

639

Amino Acids

70.32

Weight (kDa)

5.68

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 59 - 105 5.1e-17 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 70, 121
AatII GACGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 803
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 2 cut(s) 219, 713
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 1728
AccII CGCG 2 cut(s) 98, 1836
AclWI GGATC 3 cut(s) 188, 1808, 1845
AcoI YGGCCR 2 cut(s) 1008, 1188
AcsI RAATTY 2 cut(s) 248, 1198
AcuI CTGAAG 2 cut(s) 634, 1498
AcyI GRCGYC 2 cut(s) 65, 201
AfaI GTAC 4 cut(s) 146, 221, 939, 1439
AfiI CCNNNNNNNGG 5 cut(s) 36, 307, 363, 1156, 1327
AflIII ACRYGT 2 cut(s) 171, 1383
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 5 cut(s) 377, 989, 1174, 1244, 1583
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 3 cut(s) 258, 736, 1236
AluBI AGCT 6 cut(s) 527, 667, 734, 746, 872, 952
AluI AGCT 6 cut(s) 527, 667, 734, 746, 872, 952
Alw21I GWGCWC 4 cut(s) 736, 796, 954, 1861
Alw26I GTCTC 4 cut(s) 78, 1241, 1490, 1662
Alw44I GTGCAC 1 cut(s) 1857
AlwI GGATC 3 cut(s) 188, 1808, 1845
Ama87I CYCGRG 2 cut(s) 193, 623
AoxI GGCC 4 cut(s) 15, 627, 1008, 1188
ApaLI GTGCAC 1 cut(s) 1857
ApeKI GCWGC 4 cut(s) 785, 848, 869, 1529
ApoI RAATTY 2 cut(s) 248, 1198
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
AsiGI ACCGGT 1 cut(s) 56
Asp718I GGTACC 1 cut(s) 219
AspLEI GCGC 3 cut(s) 230, 435, 929
AspS9I GGNCC 6 cut(s) 16, 627, 968, 1705, 1877, 1904
AsuC2I CCSGG 4 cut(s) 14, 38, 194, 195
AsuHPI GGTGA 4 cut(s) 533, 776, 1293, 1460
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 2 cut(s) 193, 623
AvaII GGWCC 4 cut(s) 968, 1705, 1877, 1904
BaeGI GKGCMC 2 cut(s) 718, 1861
BalI TGGCCA 1 cut(s) 1010
BanI GGYRCC 2 cut(s) 219, 713
BanII GRGCYC 2 cut(s) 736, 954
BbsI GAAGAC 2 cut(s) 117, 1751
Bbv12I GWGCWC 4 cut(s) 736, 796, 954, 1861
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 4 cut(s) 772, 835, 881, 1541
BccI CCATC 5 cut(s) 388, 899, 917, 1513, 1605
BceAI ACGGC 3 cut(s) 10, 1175, 1203
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 3 cut(s) 260, 738, 1238
BciVI GTATCC 1 cut(s) 1373
BcnI CCSGG 4 cut(s) 14, 38, 194, 195
BcoDI GTCTC 4 cut(s) 78, 1241, 1490, 1662
BfaI CTAG 6 cut(s) 603, 668, 1400, 1443, 1574, 1619
BfmI CTRYAG 3 cut(s) 184, 272, 558
BfoI RGCGCY 1 cut(s) 930
BfuAI ACCTGC 1 cut(s) 803
BfuI GTATCC 1 cut(s) 1373
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 9 cut(s) 23, 96, 99, 102, 105, 786, 849, 870, 1530
BlsI GCNGC 9 cut(s) 24, 97, 100, 103, 106, 787, 850, 871, 1531
Bme1390I CCNGG 7 cut(s) 14, 38, 194, 195, 260, 738, 1238
Bme18I GGWCC 4 cut(s) 968, 1705, 1877, 1904
BmeT110I CYCGRG 2 cut(s) 193, 623
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 6 cut(s) 16, 627, 968, 1705, 1877, 1904
BmiI GGNNCC 8 cut(s) 221, 461, 590, 628, 715, 969, 1878, 1906
BmrFI CCNGG 7 cut(s) 14, 38, 194, 195, 260, 738, 1238
BmrI ACTGGG 1 cut(s) 1595
BmsI GCATC 6 cut(s) 252, 670, 772, 852, 907, 1691
BmtI GCTAGC 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 1595
BpiI GAAGAC 2 cut(s) 117, 1751
BpmI CTGGAG 2 cut(s) 810, 828
Bpu10I CCTNAGC 2 cut(s) 930, 1690
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 4 cut(s) 14, 38, 194, 195
Bsa29I ATCGAT 3 cut(s) 51, 1807, 1849
BsaAI YACGTR 1 cut(s) 662
BsaHI GRCGYC 2 cut(s) 65, 201
BsaI GGTCTC 1 cut(s) 1241
BsaJI CCNNGG 7 cut(s) 12, 96, 193, 436, 1027, 1155, 1414
BsaWI WCCGGW 4 cut(s) 56, 216, 337, 1853
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 505, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 307, 363, 1156, 1327
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 4 cut(s) 1112, 1590, 1695, 1707
Bse3DI GCAATG 3 cut(s) 298, 405, 1539
BseBI CCWGG 3 cut(s) 260, 738, 1238
BseCI ATCGAT 3 cut(s) 51, 1807, 1849
BseDI CCNNGG 7 cut(s) 12, 96, 193, 436, 1027, 1155, 1414
BseGI GGATG 7 cut(s) 42, 399, 685, 919, 940, 1616, 1644
BseLI CCNNNNNNNGG 5 cut(s) 36, 307, 363, 1156, 1327
BseMI GCAATG 3 cut(s) 298, 405, 1539
BseMII CTCAG 6 cut(s) 89, 339, 542, 629, 921, 1692
BseNI ACTGG 4 cut(s) 1112, 1590, 1695, 1707
BseRI GAGGAG 4 cut(s) 176, 179, 452, 526
BseSI GKGCMC 2 cut(s) 718, 1861
BseX3I CGGCCG 1 cut(s) 1188
BseXI GCAGC 4 cut(s) 772, 835, 881, 1541
Bsh1236I CGCG 2 cut(s) 98, 1836
Bsh1285I CGRYCG 1 cut(s) 1191
BshFI GGCC 4 cut(s) 17, 629, 1010, 1190
BshNI GGYRCC 2 cut(s) 219, 713
BshTI ACCGGT 1 cut(s) 56
BshVI ATCGAT 3 cut(s) 51, 1807, 1849
BsiEI CGRYCG 1 cut(s) 1191
BsiHKAI GWGCWC 4 cut(s) 736, 796, 954, 1861
BsiHKCI CYCGRG 2 cut(s) 193, 623
BsiSI CCGG 8 cut(s) 14, 37, 57, 178, 194, 217, 338, 1854
BslFI GGGAC 3 cut(s) 53, 981, 1354
BslI CCNNNNNNNGG 5 cut(s) 36, 307, 363, 1156, 1327
BsmAI GTCTC 4 cut(s) 78, 1241, 1490, 1662
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 3 cut(s) 53, 981, 1354
BsmI GAATGC 4 cut(s) 358, 838, 995, 1183
BsnI GGCC 4 cut(s) 17, 629, 1010, 1190
Bso31I GGTCTC 1 cut(s) 1241
BsoBI CYCGRG 2 cut(s) 193, 623
Bsp1286I GDGCHC 5 cut(s) 718, 736, 796, 954, 1861
Bsp143I GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
BspANI GGCC 4 cut(s) 17, 629, 1010, 1190
BspCNI CTCAG 6 cut(s) 88, 340, 541, 630, 922, 1691
BspDI ATCGAT 3 cut(s) 51, 1807, 1849
BspFNI CGCG 2 cut(s) 98, 1836
BspLI GGNNCC 8 cut(s) 221, 461, 590, 628, 715, 969, 1878, 1906
BspMAI CTGCAG 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 803
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 3 cut(s) 188, 1808, 1845
BspT107I GGYRCC 2 cut(s) 219, 713
BspTNI GGTCTC 1 cut(s) 1241
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 3 cut(s) 298, 405, 1539
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 4 cut(s) 1112, 1590, 1695, 1707
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 7 cut(s) 12, 96, 193, 436, 1027, 1155, 1414
BssMI GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
BssNAI GTATAC 1 cut(s) 1729
BssNI GRCGYC 2 cut(s) 65, 201
BssT1I CCWWGG 4 cut(s) 436, 1027, 1155, 1414
Bst1107I GTATAC 1 cut(s) 1729
Bst2UI CCWGG 3 cut(s) 260, 738, 1238
Bst4CI ACNGT 4 cut(s) 562, 942, 1003, 1126
Bst6I CTCTTC 3 cut(s) 153, 1239, 1517
BstACI GRCGYC 2 cut(s) 65, 201
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 8 cut(s) 298, 317, 321, 604, 867, 962, 1012, 1871
BstDEI CTNAG 9 cut(s) 75, 348, 528, 542, 638, 930, 1678, 1690, 1799
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 7 cut(s) 42, 399, 685, 919, 940, 1616, 1644
BstFNI CGCG 2 cut(s) 98, 1836
BstH2I RGCGCY 1 cut(s) 930
BstHHI GCGC 3 cut(s) 230, 435, 929
BstKTI GATC 8 cut(s) 9, 183, 1807, 1811, 1816, 1840, 1849, 1853
BstMAI GTCTC 4 cut(s) 78, 1241, 1490, 1662
BstMBI GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
BstMCI CGRYCG 1 cut(s) 1191
BstNI CCWGG 3 cut(s) 260, 738, 1238
BstNSI RCATGY 5 cut(s) 323, 964, 1387, 1735, 1869
BstSCI CCNGG 7 cut(s) 12, 36, 192, 193, 258, 736, 1236
BstSFI CTRYAG 3 cut(s) 184, 272, 558
BstSLI GKGCMC 2 cut(s) 718, 1861
BstUI CGCG 2 cut(s) 98, 1836
BstV1I GCAGC 4 cut(s) 772, 835, 881, 1541
BstV2I GAAGAC 2 cut(s) 117, 1751
BstX2I RGATCY 1 cut(s) 180
BstXI CCANNNNNNTGG 1 cut(s) 1702
BstYI RGATCY 1 cut(s) 180
BstZ17I GTATAC 1 cut(s) 1729
BstZI CGGCCG 1 cut(s) 1188
Bsu15I ATCGAT 3 cut(s) 51, 1807, 1849
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 4 cut(s) 17, 629, 1010, 1190
BsuTUI ATCGAT 3 cut(s) 51, 1807, 1849
BtgI CCRYGG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 7 cut(s) 42, 399, 685, 919, 940, 1616, 1644
BtsI GCAGTG 2 cut(s) 281, 1098
BtsIMutI CAGTG 4 cut(s) 281, 999, 1098, 1700
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 8 cut(s) 298, 317, 321, 604, 867, 962, 1012, 1871
CfoI GCGC 3 cut(s) 230, 435, 929
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 6 cut(s) 16, 627, 968, 1705, 1877, 1904
Cfr42I CCGCGG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 193
ClaI ATCGAT 3 cut(s) 51, 1807, 1849
CseI GACGC 1 cut(s) 209
Csp6I GTAC 4 cut(s) 145, 220, 938, 1438
CspAI ACCGGT 1 cut(s) 56
CspCI CAANNNNNGTGG 2 cut(s) 245, 280
CviQI GTAC 4 cut(s) 145, 220, 938, 1438
DdeI CTNAG 9 cut(s) 75, 348, 528, 542, 638, 930, 1678, 1690, 1799
DpnI GATC 8 cut(s) 8, 182, 1806, 1810, 1815, 1839, 1848, 1852
DpnII GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
DrdI GACNNNNNNGTC 2 cut(s) 70, 121
DseDI GACNNNNNNGTC 2 cut(s) 70, 121
EaeI YGGCCR 2 cut(s) 1008, 1188
EagI CGGCCG 1 cut(s) 1188
Eam1104I CTCTTC 3 cut(s) 153, 1239, 1517
EarI CTCTTC 3 cut(s) 153, 1239, 1517
Ecl136II GAGCTC 2 cut(s) 734, 952
EclXI CGGCCG 1 cut(s) 1188
Eco130I CCWWGG 4 cut(s) 436, 1027, 1155, 1414
Eco24I GRGCYC 2 cut(s) 736, 954
Eco31I GGTCTC 1 cut(s) 1241
Eco47I GGWCC 4 cut(s) 968, 1705, 1877, 1904
Eco52I CGGCCG 1 cut(s) 1188
Eco53kI GAGCTC 2 cut(s) 734, 952
Eco57I CTGAAG 2 cut(s) 634, 1498
Eco88I CYCGRG 2 cut(s) 193, 623
EcoICRI GAGCTC 2 cut(s) 734, 952
EcoO109I RGGNCCY 2 cut(s) 968, 1877
EcoRII CCWGG 3 cut(s) 258, 736, 1236
EcoT14I CCWWGG 4 cut(s) 436, 1027, 1155, 1414
EcoT22I ATGCAT 3 cut(s) 325, 997, 1183
EcoT38I GRGCYC 2 cut(s) 736, 954
ErhI CCWWGG 4 cut(s) 436, 1027, 1155, 1414
Esp3I CGTCTC 1 cut(s) 78
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 3 cut(s) 53, 981, 1354
FauI CCCGC 1 cut(s) 219
FblI GTMKAC 1 cut(s) 1728
Fnu4HI GCNGC 9 cut(s) 23, 96, 99, 102, 105, 786, 849, 870, 1530
FokI GGATG 7 cut(s) 29, 406, 692, 926, 947, 1623, 1651
FriOI GRGCYC 2 cut(s) 736, 954
Fsp4HI GCNGC 9 cut(s) 23, 96, 99, 102, 105, 786, 849, 870, 1530
FspBI CTAG 6 cut(s) 603, 668, 1400, 1443, 1574, 1619
GlaI GCGC 3 cut(s) 229, 434, 928
GluI GCNGC 9 cut(s) 23, 96, 99, 102, 105, 786, 849, 870, 1530
GsuI CTGGAG 2 cut(s) 810, 828
HaeII RGCGCY 1 cut(s) 930
HaeIII GGCC 4 cut(s) 17, 629, 1010, 1190
HapII CCGG 8 cut(s) 14, 37, 57, 178, 194, 217, 338, 1854
HgaI GACGC 1 cut(s) 209
HhaI GCGC 3 cut(s) 230, 435, 929
Hin1I GRCGYC 2 cut(s) 65, 201
Hin6I GCGC 3 cut(s) 228, 433, 927
HinP1I GCGC 3 cut(s) 228, 433, 927
HincII GTYRAC 1 cut(s) 1122
HindII GTYRAC 1 cut(s) 1122
HinfI GANTC 2 cut(s) 1142, 1432
HpaII CCGG 8 cut(s) 14, 37, 57, 178, 194, 217, 338, 1854
HphI GGTGA 4 cut(s) 533, 776, 1293, 1460
Hpy166II GTNNAC 8 cut(s) 256, 1122, 1271, 1301, 1440, 1700, 1729, 1859
Hpy188I TCNGA 7 cut(s) 531, 639, 1065, 1210, 1357, 1813, 1904
Hpy188III TCNNGA 5 cut(s) 77, 377, 878, 911, 1321
Hpy8I GTNNAC 8 cut(s) 256, 1122, 1271, 1301, 1440, 1700, 1729, 1859
Hpy99I CGWCG 8 cut(s) 70, 73, 112, 115, 118, 133, 152, 203
HpyAV CCTTC 4 cut(s) 692, 822, 1461, 1840
HpyCH4III ACNGT 4 cut(s) 562, 942, 1003, 1126
HpyCH4IV ACGT 3 cut(s) 65, 171, 661
HpyF3I CTNAG 9 cut(s) 75, 348, 528, 542, 638, 930, 1678, 1690, 1799
HpySE526I ACGT 3 cut(s) 65, 171, 661
Hsp92I GRCGYC 2 cut(s) 65, 201
HspAI GCGC 3 cut(s) 228, 433, 927
KpnI GGTACC 1 cut(s) 223
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
LmnI GCTCC 5 cut(s) 219, 465, 739, 791, 809
Lsp1109I GCAGC 4 cut(s) 772, 835, 881, 1541
LweI GCATC 6 cut(s) 252, 670, 772, 852, 907, 1691
MaeI CTAG 6 cut(s) 603, 668, 1400, 1443, 1574, 1619
MaeII ACGT 3 cut(s) 65, 171, 661
MaeIII GTNAC 3 cut(s) 1165, 1192, 1360
MalI GATC 8 cut(s) 8, 182, 1806, 1810, 1815, 1839, 1848, 1852
MbiI CCGCTC 1 cut(s) 214
MboI GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 5 cut(s) 718, 736, 796, 954, 1861
MlsI TGGCCA 1 cut(s) 1010
MluCI AATT 6 cut(s) 248, 422, 1056, 1198, 1500, 1722
MluNI TGGCCA 1 cut(s) 1010
MlyI GAGTC 1 cut(s) 1151
MmeI TCCRAC 3 cut(s) 1043, 1699, 1882
Mox20I TGGCCA 1 cut(s) 1010
Mph1103I ATGCAT 3 cut(s) 325, 997, 1183
MscI TGGCCA 1 cut(s) 1010
MseI TTAA 3 cut(s) 1184, 1557, 1842
MslI CAYNNNNRTG 4 cut(s) 291, 660, 1603, 1864
Msp20I TGGCCA 1 cut(s) 1010
MspA1I CMGCKG 2 cut(s) 32, 98
MspI CCGG 8 cut(s) 14, 37, 57, 178, 194, 217, 338, 1854
MspR9I CCNGG 7 cut(s) 14, 38, 194, 195, 260, 738, 1238
MunI CAATTG 1 cut(s) 1056
Mva1269I GAATGC 4 cut(s) 358, 838, 995, 1183
MvaI CCWGG 3 cut(s) 260, 738, 1238
MvnI CGCG 2 cut(s) 98, 1836
NciI CCSGG 4 cut(s) 14, 38, 194, 195
NdeII GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
NheI GCTAGC 1 cut(s) 602
NlaIV GGNNCC 8 cut(s) 221, 461, 590, 628, 715, 969, 1878, 1906
NmuCI GTSAC 1 cut(s) 1165
NsiI ATGCAT 3 cut(s) 325, 997, 1183
NspI RCATGY 5 cut(s) 323, 964, 1387, 1735, 1869
PaeI GCATGC 2 cut(s) 323, 964
PciI ACATGT 1 cut(s) 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 4 cut(s) 358, 838, 995, 1183
PfeI GAWTC 1 cut(s) 1432
PflFI GACNNNGTC 2 cut(s) 67, 121
PfoI TCCNGGA 1 cut(s) 736
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 9 cut(s) 24, 97, 100, 103, 106, 787, 850, 871, 1531
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 2 cut(s) 968, 1877
PscI ACATGT 1 cut(s) 1383
Psp124BI GAGCTC 2 cut(s) 736, 954
Psp5II RGGWCCY 2 cut(s) 968, 1877
Psp6I CCWGG 3 cut(s) 258, 736, 1236
PspGI CCWGG 3 cut(s) 258, 736, 1236
PspN4I GGNNCC 8 cut(s) 221, 461, 590, 628, 715, 969, 1878, 1906
PspPI GGNCC 6 cut(s) 16, 627, 968, 1705, 1877, 1904
PspPPI RGGWCCY 2 cut(s) 968, 1877
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 180
PsyI GACNNNGTC 2 cut(s) 67, 121
RsaI GTAC 4 cut(s) 146, 221, 939, 1439
RsaNI GTAC 4 cut(s) 145, 220, 938, 1438
RseI CAYNNNNRTG 4 cut(s) 291, 660, 1603, 1864
SacI GAGCTC 2 cut(s) 736, 954
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 3 cut(s) 1184, 1557, 1842
SatI GCNGC 9 cut(s) 23, 96, 99, 102, 105, 786, 849, 870, 1530
Sau3AI GATC 8 cut(s) 6, 180, 1804, 1808, 1813, 1837, 1846, 1850
Sau96I GGNCC 6 cut(s) 16, 627, 968, 1705, 1877, 1904
SchI GAGTC 1 cut(s) 1151
ScrFI CCNGG 7 cut(s) 14, 38, 194, 195, 260, 738, 1238
SduI GDGCHC 5 cut(s) 718, 736, 796, 954, 1861
SfaNI GCATC 6 cut(s) 252, 670, 772, 852, 907, 1691
SfcI CTRYAG 3 cut(s) 184, 272, 558
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 4 cut(s) 968, 1705, 1877, 1904
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 4 cut(s) 291, 660, 1603, 1864
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 2 cut(s) 323, 964
Sse9I AATT 6 cut(s) 248, 422, 1056, 1198, 1500, 1722
SspMI CTAG 6 cut(s) 603, 668, 1400, 1443, 1574, 1619
SstI GAGCTC 2 cut(s) 736, 954
StyD4I CCNGG 7 cut(s) 12, 36, 192, 193, 258, 736, 1236
StyI CCWWGG 4 cut(s) 436, 1027, 1155, 1414
TaaI ACNGT 4 cut(s) 562, 942, 1003, 1126
TaiI ACGT 3 cut(s) 68, 174, 664
TaqI TCGA 4 cut(s) 51, 1765, 1807, 1849
TasI AATT 6 cut(s) 248, 422, 1056, 1198, 1500, 1722
TauI GCSGC 5 cut(s) 25, 98, 101, 104, 107
TfiI GAWTC 1 cut(s) 1432
Tru1I TTAA 3 cut(s) 1184, 1557, 1842
Tru9I TTAA 3 cut(s) 1184, 1557, 1842
TscAI CASTG 4 cut(s) 281, 1006, 1105, 1707
TseFI GTSAC 1 cut(s) 1165
TseI GCWGC 4 cut(s) 785, 848, 869, 1529
Tsp45I GTSAC 1 cut(s) 1165
TspDTI ATGAA 3 cut(s) 416, 711, 968
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 4 cut(s) 281, 1006, 1105, 1707
Tth111I GACNNNGTC 2 cut(s) 67, 121
VneI GTGCAC 1 cut(s) 1857
VpaK11BI GGWCC 4 cut(s) 968, 1705, 1877, 1904
XapI RAATTY 2 cut(s) 248, 1198
XceI RCATGY 5 cut(s) 323, 964, 1387, 1735, 1869
XcmI CCANNNNNNNNNTGG 1 cut(s) 1715
XmaI CCCGGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 1728
XspI CTAG 6 cut(s) 603, 668, 1400, 1443, 1574, 1619
ZraI GACGTC 1 cut(s) 66
Zsp2I ATGCAT 3 cut(s) 325, 997, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.