pycom05g27410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005526
Physical Location & Seq
Forward (+)
28396844 .. 28397134
291 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27410.1

Sequence Viewer

Length: 291 bp
ATGCACTCCGTCGGTCGGAAACCCTTTTCTTTCTTCTTAACCAGTATATTAATATTTCTCTTAGTTTTGGCACCCATGGCATCTGCAAGTCGAACCTACCCAACACCAAATGTACTGGATTGTAAAAGCCAAGGCGCTTCGGAGACGCCTGCAGCTGTGCCTCGGCTGGTGCTGTTAGATTTGCAGGAGAGGCTAGTGCCTGTTTTTCATCATGTTATTTCTAAGCGTCCTGTCCTGCCTTCTGGTCCAAGTCATAGGAGTAACAAGGCTCCCAATTCCTCAAGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.52

Weight (kDa)

10.92

Isoelectric Point (pI)

68.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 15
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 152
AseI ATTAAT 1 cut(s) 50
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 245
AvaII GGWCC 1 cut(s) 245
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 72, 270
BmsI GCATC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 75, 130, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 2 cut(s) 42, 120
BseDI CCNNGG 3 cut(s) 75, 130, 161
BseLI CCNNNNNNNGG 1 cut(s) 15
BseNI ACTGG 2 cut(s) 42, 120
BseXI GCAGC 1 cut(s) 164
Bsh1285I CGRYCG 1 cut(s) 16
BshNI GGYRCC 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 137
BsmBI CGTCTC 1 cut(s) 137
Bsp19I CCATGG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 72, 270
BspMAI CTGCAG 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 70
BsrI ACTGG 2 cut(s) 42, 120
BssECI CCNNGG 3 cut(s) 75, 130, 161
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 75, 130
BstACI GRCGYC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 61, 222
BstDSI CCRYGG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstMAI GTCTC 1 cut(s) 137
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 77, 190
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 150
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 2 cut(s) 154, 215
Csp6I GTAC 1 cut(s) 113
CviAII CATG 2 cut(s) 76, 212
CviJI RGCY 5 cut(s) 129, 155, 166, 193, 269
CviKI_1 RGCY 5 cut(s) 129, 155, 166, 193, 269
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 2 cut(s) 61, 222
Eco130I CCWWGG 2 cut(s) 75, 130
Eco47I GGWCC 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 75, 130
ErhI CCWWGG 2 cut(s) 75, 130
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 2 cut(s) 79, 215
FaiI YATR 4 cut(s) 47, 77, 213, 255
FatI CATG 2 cut(s) 75, 211
Fnu4HI GCNGC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 153
HaeII RGCGCY 1 cut(s) 138
HgaI GACGC 2 cut(s) 154, 215
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 2 cut(s) 79, 215
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
Hpy188I TCNGA 2 cut(s) 18, 142
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 249
HpyCH4V TGCA 4 cut(s) 4, 86, 152, 184
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 190
HpyF3I CTNAG 2 cut(s) 61, 222
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 2 cut(s) 79, 215
HspAI GCGC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 9 cut(s) 55, 101, 152, 162, 170, 213, 228, 243, 248
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 1 cut(s) 89
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 260
MboII GAAGA 1 cut(s) 25
MluCI AATT 1 cut(s) 274
MnlI CCTC 3 cut(s) 171, 183, 289
MseI TTAA 2 cut(s) 38, 50
MspA1I CMGCKG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 77, 190
NcoI CCATGG 1 cut(s) 75
NlaIII CATG 2 cut(s) 79, 215
NlaIV GGNNCC 2 cut(s) 72, 270
NmeAIII GCCGAG 1 cut(s) 142
PkrI GCNGC 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 50
PspN4I GGNNCC 2 cut(s) 72, 270
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 38, 50
SatI GCNGC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 245
SetI ASST 2 cut(s) 98, 157
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 1 cut(s) 274
SspI AATATT 1 cut(s) 54
SspMI CTAG 1 cut(s) 194
StyI CCWWGG 2 cut(s) 75, 130
TaqI TCGA 1 cut(s) 91
TasI AATT 1 cut(s) 274
TatI WGTACW 1 cut(s) 112
Tru1I TTAA 2 cut(s) 38, 50
Tru9I TTAA 2 cut(s) 38, 50
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 50
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.