Rorug05G0579300

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000561
Physical Location & Seq
Forward (+)
77528966 .. 77555350
26385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0579300.1

Sequence Viewer

Length: 6021 bp
ATGGCTCAAGTTGAGGAGCGATGGGAGCGGTTAGTCCGCGCAGTATTGAGGAGGGAAAGGATGGGGCCTGATGCATACGAGCAGCATGGGACTGGCATTGCCGGAAATGTTCCATCTTCACTTGAAAATAATAGGGATATTGATGAAATTCTCAGAGTTGCAGATGAAATCCAAGATGAAGATCCCAATATCTCTAGAATCCAATGTGAGCATGGTTATTCGCTGGCTCAAAATCTAGACCCCAATAGTGAAGGAAGGGGAGTTTTACACTTCGAGACCGGATTGATGTCTGTCATTAAGAAAATGGCTCAAGTTGAGGATCGATGGGAGCGGTTAGTCCGCGCAGTATTGAGGAGGGAAAGGATGGGGCCTGATGCATACGAGCGGCATGGGACTGGCATTGCCGGAAATGTTCCATCTTCACTTGAAAATAATAGGGATATTGATGAAATTCTCAGAGTTGCAGATGAAATCCAAGATGAAGATCCCAATATCTCTAGAATCCTATGTGAGCATGGTTATTCGTTGGCTCAAAATCTAGACCCCAATAGTGAAGGAAGGGGAGTATTACAGTTCAAGACTGGATTGATGTCTGTCATTAAGCAAAAGCTAGCGAAGAGGGAAGGGGCAACCATAGATAGAAGCCAAGACATTGTTCGCTTACAAGAGTTCTATAAACAGTATAGACAGAAAAATAATGTTGAACAATTGCGTGAGGAAGAAATGAAGTTGAGGGAGTCTGGTGTTTTCAGTGGAAACCTGGGCGAACTGGAAAGGAAAACAGTGAAGAGGAAAAGAGTATTCGCGACTCTAAGAGTTCTGGGCAAAGTTTTGGAGCAGCTGACAGAGGACATTCCAGAAGAGCTCAAGCACCAAATGGAGATAGATGCCGCAATGACTGAAGACCTGATTGCGTACAATATTATTCCACTGGATGCTCCTAGCATTACAAATGTTATCATGTCCTTGGCTGAGGTTCAAGCAGCAGTTTCTGGGTTAAAGTACTTCAGGGGCCTACCAAAGTTACCGACAGATTTTCCAATTCCTGCTACACGAGAGCCTGACATGCTTGATTTCCTGCATTATGTATTTGGATTTCAGAAAGACAATGTTTGTAACCAGCGCGAACATATTGTGCATCTCCTTGCAAATGAGCAATCACGGCTTCGTATTCCTGAAGAAACTGAACCTATATTGGATGAGGCTGCGGTGCAAAATATATTTTTAAGGTCCCTAGACAACTATATTAAGTGGTGTAACTACTTGTGCATTCAGCCAGTTTGGAGCAATTTGCAGTCAGTTAGCAAGGAGAAAAAGCTACTGTTTGTTTCCATGTACTGTTTGATCTGGGGTGAAGCAGCCAATGTTAGATTTCTTCCAGAATGCTTGTGCTACATATTTCATCATATGGCAAGGGAAATGGATGAAATTCTGAGGCAGCAGACTGCACAGCCAGCCAACAGTTGTAATTCTGAAAATGATGGAGTTTCTTTCCTTGATCAAGTTATATATCCTCTGTTTGAGATTGTGTCTGCGGAAGCTGGAAACAATGAAAATGGACGAGCACCACATTCTGCTTGGCGCAATTATGATGATTTTAATGAGTACTTCTGGTCACTCCATTGTTTTGAACTTAGTTGGCCCTGGCGCAAAGGTTCATCATTTTTTCAAAAGCCAACGCCAAGGTCAAAGAATATTCTCAAATCTGGCAGAAGTCAACATCGTGGAAAAACTTCATTTGTTGAGCATAGGACATTTCTCCATCTGTATCATAGTTTCCACCGTCTGTGGATATTCCTTGTGATGATGTTTCAGGGACTTGCGATCATTGCATTCAATGGAAGTTTTGATGCAAAATGTATTAGAGAAATTCTCAGTCTCGGTCCGACATTTGTTGGAATGAAATTTCTCGAGAGTGTTCTTGATGTTCTTATGATGTATGGCGCCTATTCTACAACACGGCGCCTAGCAGTTTCTCGAATTTTTCTCCGATTTGTTTGGTTTGGCACTGCATCAGTGGTCATATCTTTTCTTTATGTGAAAGCTCTCCAGGAAGAGAGTAAACAAAATGGAAACCCTATTATCTACAAGTTGTATCTGCTCATTGTAGGCATATATGCTGGTATCCAGTTCTTCATTAGTTTTTTCATGCGCATACCAGCCTGCCACAATCTGACTAATCAATGTGATCGCTGGCCTCTGATTCGCCTTGTCAAGTGGATGCGCCAGGAACGGTACTATGTTGGACGGGGCATGTATGAGAGGACTACTGATTTCATCAAGTACATGTTTTTCTGGCTTGTGATTTTGAGTGGAAAGTTTTCATTTGCATACTTTCTCCAGATCAAGCCGTTGGTGGAACCAACCAGAGTTATAGTGAACACAAATGCTATAACGTATACTTGGCATGATTTTGTTTCTAAAAACAACTATAATGCTCTGACAGTTGCTGCTTTGTGGGCTCCCGTGATAATTATTTATCTTTTAGATCTCTATGTATTTTACACCGTTATATCTGCTGTGTGGGGATTCTTGCTTGGTGCCAGAGATCGTCTTGGAGAGATTAGATCATTGGAAGCACTTCATAAACTATTTGAGCAGTTTCCGGGAGCTTTTATGGACACTCTTCATATCCGCCTGCCTAACAGGGCTTACCATCAGTCTTCCAGTGAGGATATTGAGAAGAACAAGGTTGATGCTAGTCGATTTTCTCCCTTTTGGAATGAAATCATAAAAAATCTGAGGGAAGAAGATTATATCACTGACCTAGAAATGGAGCTGCTTGTAATGCCTAAAAATTCTGGAAGTCTTCCCTTAGTTCAGTGGCCCCTTTTCCTTCTTGCTAGCAAGATATTTGTTGCCAAAGATATTGCTCTTGAAACCAGGGATTCACAAGATGAGCTTTGGGAAAGAATTTCTAGAGATGACTATATGAAGTATGCTGTCCAGGAGTGCTTCCATAGCATTAAGCTTATCCTAAGTGAAATATTGGAGGGTGAAGGAAAGCTGTGGGTTGAAAGGATATATGATGATATTCAAGTAAGCATAGTGAAGAAGAGTATTCAGGCTGACTTTCAACTGAACAAGCTGCCACTCGTGATTTCAAGAGTTACTGCATTAATGGGAATTCTGAAAAAAGAGGAAACATCTGAACTGGTGGATGGTGCTATTAAGGCTGTTCAAGATCTCTACGACATTGTGCGCCATGATGTCCTTTCTATTAACATGAGGGAAAACTATGAAACATGGAATTTGTTGTCAAAAGCACGGACGGATGGTCGGTTGTTTGCCAAGTTGAAGTGGCCCAAGGATCCTTCATTGAGAGCACAAGTCAAAAGATTGTATTCATTGTTGACAATTCAAGATTCAGCTGCCAATGTCCCCCAAAATCTGGAGGCCGGACGTAGGCTGGAGTTCTTTACAAATTCTCTTTTCATGGAGATGCCCGAAGCAAGGCCAGTCCGTGAAATGTTGTCGTTCAGTGTTTTTACTCCATATTATGCTGAGACTGTGCTCTATAGTATCGCTGAACTCCAGAAGAAAAATGAAGATGGGATATCAGTATTGTTTTACCTTCAAAAGATTTACCCAGATGAGTGGAAGAACTTTCTTTTACGGATTGGCCGTGACGAAAATGCACATGACTCGGAACTTTATGATAATCCCTCTGATATTCTTGAGCTTCGGTTTTGGGCGTCTTACCGAGGCCAAACATTGGCCAGAACTGTTCGTGGGATGATGTATTATAGGAAGGCTCTTATGCTTCAGACCTATTTGGAGAGATTGAATTCTGGAGTATCAGATGTGGAGGCTGCAATTTCTAGTGGTGATGCGGCTGAAACTCGAGCATTTGAGTTATCTCCTGAAGCACGAGCTCAGGCAGACCTGAAGTTTACATATGTAGTTACTTGTCAAATATATGGAAAACAGAAAGAAGGCCAAAAACCTGAGGCTGCTGATATAGCATTGCTTATGCAAAGAAATGAGGCTCTTCGAGTTGCTTTTATCGATGAGGTTGAGACTTTGAAGGATGGTAAAGTGAACAAAGAGTTCTATTCAAAACTTGTAAAGGCAGATATCAATGGGAAGGACAAGGAAATCTACTCTATAAAACTGCCTGGAAATCCAAAAATAGGTGAAGGGAAACCGGAGAACCAAAATCATGCAATTGTATTTACTCGTGGAAATGCAATTCAGACAATTGACATGAATCAGGATAACTATTTTGAGGAAGCTTTGAAGATGAGAAATTTACTCGAAGAATTCCACCGTGATCATGGTATCCGTCGTCCTACAATTCTTGGTGTCAGGGAACATGTCTTTACGGGAAGTGTTTCTTCTTTAGCGTCATTCATGTCCAATCAGGAAACAAGCTTTGTAACTCTAGCTCAGCGGGTTCTTGCCAATCCTTTGAAGGTCCGTATGCATTATGGTCATCCTGATGTGTTTGATAGAGTTTTCCACATAACACGGGGTGGTATTAGCAAGGCTTCTCGAGTGATTAACATCAGTGAAGACATCTTTGCAGGCTTCAACTCAACTTTACGCCAAGGAAATGTGACTCACCATGAGTATATTCAGGTTGGCAAAGGGCGGGACGTTGGGCTCAACCAAATTGCAGTATTTGAGGGGAAGGTTGCTGGTGGTAATGGTGAACAGGTTCTGAGTCGTGATGTATACAGGCTTGGGCAGCAGTTTGATTTCTTCCGCATGCTGTCATTTTACTTTACCACTGTCGGCTACTACTTCTGTACAATGTTAACAGTGCTGACGGTTTACATTTTTCTCTACGGGAAAACATATATGGCGCTATCTGGGGTTGGTGAATACTTGCAGAACGTAGCTGAGGTAACCAAGAATACCGCACTGACTGCTGCTCTAAATACACAATTCCTCCTTCAAATTGGTATCTTTACTGCTGTCCCGATGATTTTAGGCTCCATCTTGGAACAGGGTTTCCTGCGGGCCATAGTAACTTTCCTCACAATGCAGTTGCAACTTTGTTCTGTCTTCTTCACATTCTCCTTGGGAACCAAAACACATTATTTTGGCCGGACAATTCTCCATGGTGGTGCAAAGTATCAAGCAACCGGCAGGGGATTTGTAGTCCGGCATGTTAAGTTTACAGAGAATTACAGGCTTTACTCCCGCAGTCATTTTACCAAAGGGTTGGAAGTAGTTCTTTTGTTGGTAGTGTACCTTGCTTATGGATATGATGATGGCGGTGCTATATCATACATTCTTCTTTCAATAACCAGTTGGTTCATGGCTCTTTCTTGGCTTTTTGCTCCCTATTTGTTCAACCCATCTGGGTTTGAGTGGCAGAAGGTTGTGGAGGATTTCCGAGATTGGACAAATTGGCTTCTTTATAGAGGTGGAATTGGAGTTAAGGGTGAAGAAAGCTGGGAAGCTTGGTGGGAGGAAGAACTGGCGCATATTAGAACCTTGGGAGGAAGGATTGCAGAGACAATTTTGAGTCTAAGATTCTTTATCTTCCAGTATGGAATTATCTACCGGCTAGATGTGAAGGGATCAGATACATCACTAACGGTGTATGGCCTCTCATGGATCGTGCTGGCGGTGCTTATAATTCTTTTCAAGGTTTTCACCTTCAGCCAGAAGATATCTGTCAACTTCCAGCTTCTGTTGCGCTTCATTCAAGGGGTCGCCTTCATGTTGGCACTGGCTGGTTTAGCAGTAGCAGTCAAATTTACAGATCTGACAATCGCAGACGTTTTTGCTTCCATTTTAGCGTTTGTACCCACTGGGTGGGGAATTCTTTCCATTTGCATAGCTTGGAAACCCTTGATGAAGAAACTGGGCTTGTGGAAATCCATCCGTTCCATTGCCCTTCTTTATGATGCTGGGATGGGTATGGTTATATTCATTCCCATTGCGTTGTCATCATGGTTCCCATTTGTATCAACGTTTCAAGCTCGGCTCATGTTCAACCAGGCTTTCAGTCGAGGGTTGGAAATCTCTGTGCTACTTGCTGGAAACAACCCCAATTCTGGATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

2006

Amino Acids

230.5

Weight (kDa)

6.83

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 439 - 547 5.9e-40 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 943 - 1120 6.5e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1127 - 1817 1.3e-232 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5588
Acc16I TGCGCA 1 cut(s) 2153
AccB1I GGYRCC 3 cut(s) 1943, 1962, 2540
AccB7I CCANNNNNTGG 3 cut(s) 3391, 3715, 5769
AccBSI CCGCTC 3 cut(s) 28, 331, 385
AccI GTMKAC 2 cut(s) 2399, 4677
AccII CGCG 4 cut(s) 39, 342, 806, 1125
AclI AACGTT 1 cut(s) 5927
AclWI GGATC 7 cut(s) 176, 327, 479, 3305, 3318, 5539, 5576
AcoI YGGCCR 3 cut(s) 3622, 3717, 5050
AcuI CTGAAG 7 cut(s) 921, 991, 1197, 3749, 3885, 3908, 5596
AcyI GRCGYC 3 cut(s) 1944, 1963, 3695
AdeI CACNNNGTG 2 cut(s) 4475, 5769
AfaI GTAC 9 cut(s) 917, 1004, 1337, 1607, 2237, 2285, 4753, 5198, 5760
AfiI CCNNNNNNNGG 8 cut(s) 2773, 3391, 3405, 3453, 3715, 4133, 5769, 6011
AflIII ACRYGT 2 cut(s) 2286, 4315
AhdI GACNNNNNGTC 1 cut(s) 3276
AjnI CCWGG 8 cut(s) 759, 1643, 2049, 2226, 2882, 2946, 4117, 5952
AjuI GAANNNNNNNTTGG 2 cut(s) 639, 671
AloI GAACNNNNNNTCC 2 cut(s) 4941, 4973
Alw21I GWGCWC 5 cut(s) 867, 1567, 3328, 3516, 3877
Alw26I GTCTC 5 cut(s) 269, 1883, 3500, 4013, 5459
AlwI GGATC 7 cut(s) 176, 327, 479, 3305, 3318, 5539, 5576
AlwNI CAGNNNCTG 4 cut(s) 992, 2450, 4663, 5644
Ama87I CYCGRG 3 cut(s) 1910, 3843, 4494
AseI ATTAAT 1 cut(s) 3119
Asp700I GAANNNNTTC 7 cut(s) 1732, 2320, 2580, 4333, 4659, 5178, 5779
AsuC2I CCSGG 1 cut(s) 2607
AsuHPI GGTGA 9 cut(s) 1364, 3008, 3839, 4148, 4556, 4664, 4835, 5405, 5599
AsuNHI GCTAGC 2 cut(s) 610, 2843
AvaI CYCGRG 3 cut(s) 1910, 3843, 4494
AvaII GGWCC 3 cut(s) 1230, 1883, 4417
AxyI CCTNAGG 1 cut(s) 3948
BalI TGGCCA 1 cut(s) 3719
BamHI GGATCC 1 cut(s) 3310
BanI GGYRCC 3 cut(s) 1943, 1962, 2540
BanII GRGCYC 4 cut(s) 867, 2464, 3877, 4608
BauI CACGAG 4 cut(s) 1053, 3095, 3870, 4179
BbsI GAAGAC 5 cut(s) 909, 2655, 2801, 4521, 5002
Bbv12I GWGCWC 5 cut(s) 867, 1567, 3328, 3516, 3877
BbvCI CCTCAGC 2 cut(s) 972, 4845
BceAI ACGGC 4 cut(s) 1178, 1976, 2335, 3609
BcgI CGANNNNNNTGC 2 cut(s) 3627, 3661
BciT130I CCWGG 8 cut(s) 761, 1645, 2051, 2228, 2884, 2948, 4119, 5954
BciVI GTATCC 2 cut(s) 2135, 4292
BclI TGATCA 2 cut(s) 1498, 4273
BcnI CCSGG 1 cut(s) 2607
BcoDI GTCTC 5 cut(s) 269, 1883, 3500, 4013, 5459
BfmI CTRYAG 1 cut(s) 3517
BfoI RGCGCY 3 cut(s) 1947, 1966, 4811
BfuI GTATCC 2 cut(s) 2135, 4292
BglII AGATCT 3 cut(s) 2488, 3184, 5716
BlpI GCTNAGC 1 cut(s) 4389
BmcAI AGTACT 2 cut(s) 1004, 1607
Bme1390I CCNGG 9 cut(s) 761, 1645, 2051, 2228, 2607, 2884, 2948, 4119, 5954
Bme18I GGWCC 3 cut(s) 1230, 1883, 4417
BmeRI GACNNNNNGTC 1 cut(s) 3276
BmeT110I CYCGRG 3 cut(s) 1910, 3843, 4494
BmrFI CCNGG 9 cut(s) 761, 1645, 2051, 2228, 2607, 2884, 2948, 4119, 5954
BmrI ACTGGG 2 cut(s) 5775, 5828
BmtI GCTAGC 2 cut(s) 614, 2847
BmuI ACTGGG 2 cut(s) 5775, 5828
BpiI GAAGAC 5 cut(s) 909, 2655, 2801, 4521, 5002
BplI GAGNNNNNCTC 2 cut(s) 1857, 1889
BpmI CTGGAG 6 cut(s) 2033, 2324, 3413, 3431, 3518, 3813
Bpu10I CCTNAGC 3 cut(s) 972, 3876, 4845
Bpu1102I GCTNAGC 1 cut(s) 4389
BpuEI CTTGAG 3 cut(s) 294, 851, 3698
BpuMI CCSGG 1 cut(s) 2607
Bsa29I ATCGAT 2 cut(s) 322, 4008
BsaBI GATNNNNATC 3 cut(s) 180, 483, 4505
BsaHI GRCGYC 3 cut(s) 1944, 1963, 3695
BsaI GGTCTC 1 cut(s) 269
BsaWI WCCGGW 2 cut(s) 278, 4147
BsaXI ACNNNNNCTCC 2 cut(s) 4878, 4908
Bsc4I CCNNNNNNNGG 8 cut(s) 2773, 3391, 3405, 3453, 3715, 4133, 5769, 6011
Bse118I RCCGGY 2 cut(s) 5090, 5514
Bse21I CCTNAGG 1 cut(s) 3948
Bse3DI GCAATG 7 cut(s) 96, 399, 900, 1827, 3965, 5844, 5892
Bse8I GATNNNNATC 3 cut(s) 180, 483, 4505
BseBI CCWGG 8 cut(s) 761, 1645, 2051, 2228, 2884, 2948, 4119, 5954
BseCI ATCGAT 2 cut(s) 322, 4008
BseJI GATNNNNATC 3 cut(s) 180, 483, 4505
BseLI CCNNNNNNNGG 8 cut(s) 2773, 3391, 3405, 3453, 3715, 4133, 5769, 6011
BseMI GCAATG 7 cut(s) 96, 399, 900, 1827, 3965, 5844, 5892
BseRI GAGGAG 3 cut(s) 29, 64, 367
BseYI CCCAGC 2 cut(s) 5403, 5864
BsgI GTGCAG 1 cut(s) 1431
Bsh1236I CGCG 4 cut(s) 39, 342, 806, 1125
BshNI GGYRCC 3 cut(s) 1943, 1962, 2540
BshVI ATCGAT 2 cut(s) 322, 4008
BsiHKAI GWGCWC 5 cut(s) 867, 1567, 3328, 3516, 3877
BsiHKCI CYCGRG 3 cut(s) 1910, 3843, 4494
BslFI GGGAC 7 cut(s) 103, 406, 1216, 1830, 3365, 4610, 4907
BslI CCNNNNNNNGG 8 cut(s) 2773, 3391, 3405, 3453, 3715, 4133, 5769, 6011
BsmAI GTCTC 5 cut(s) 269, 1883, 3500, 4013, 5459
BsmFI GGGAC 7 cut(s) 103, 406, 1216, 1830, 3365, 4610, 4907
BsmI GAATGC 3 cut(s) 1269, 1388, 1832
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 3 cut(s) 1910, 3843, 4494
Bsp1286I GDGCHC 7 cut(s) 867, 1567, 2464, 3328, 3516, 3877, 4608
Bsp1407I TGTACA 1 cut(s) 4751
Bsp1720I GCTNAGC 1 cut(s) 4389
Bsp19I CCATGG 1 cut(s) 5065
Bsp68I TCGCGA 1 cut(s) 806
BspDI ATCGAT 2 cut(s) 322, 4008
BspFNI CGCG 4 cut(s) 39, 342, 806, 1125
BspOI GCTAGC 2 cut(s) 614, 2847
BspPI GGATC 7 cut(s) 176, 327, 479, 3305, 3318, 5539, 5576
BspQI GCTCTTC 2 cut(s) 855, 3996
BspT107I GGYRCC 3 cut(s) 1943, 1962, 2540
BspTNI GGTCTC 1 cut(s) 269
BsrBI CCGCTC 3 cut(s) 28, 331, 385
BsrDI GCAATG 7 cut(s) 96, 399, 900, 1827, 3965, 5844, 5892
BsrFI RCCGGY 2 cut(s) 5090, 5514
BsrGI TGTACA 1 cut(s) 4751
BssAI RCCGGY 2 cut(s) 5090, 5514
BssNAI GTATAC 2 cut(s) 2400, 4678
BssNI GRCGYC 3 cut(s) 1944, 1963, 3695
BssSI CACGAG 4 cut(s) 1053, 3095, 3870, 4179
BssT1I CCWWGG 7 cut(s) 966, 1682, 3306, 4549, 5025, 5065, 5445
Bst1107I GTATAC 2 cut(s) 2400, 4678
Bst2BI CACGAG 4 cut(s) 1053, 3095, 3870, 4179
Bst2UI CCWGG 8 cut(s) 761, 1645, 2051, 2228, 2884, 2948, 4119, 5954
Bst6I CTCTTC 7 cut(s) 611, 782, 855, 2049, 2631, 3050, 3996
BstACI GRCGYC 3 cut(s) 1944, 1963, 3695
BstAPI GCANNNNNTGC 1 cut(s) 4871
BstAUI TGTACA 1 cut(s) 4751
BstDSI CCRYGG 1 cut(s) 5065
BstEII GGTNACC 1 cut(s) 4849
BstFNI CGCG 4 cut(s) 39, 342, 806, 1125
BstH2I RGCGCY 3 cut(s) 1947, 1966, 4811
BstMAI GTCTC 5 cut(s) 269, 1883, 3500, 4013, 5459
BstNI CCWGG 8 cut(s) 761, 1645, 2051, 2228, 2884, 2948, 4119, 5954
BstNSI RCATGY 6 cut(s) 1069, 2257, 2290, 4319, 4714, 5117
BstPI GGTNACC 1 cut(s) 4849
BstSCI CCNGG 9 cut(s) 759, 1643, 2049, 2226, 2605, 2882, 2946, 4117, 5952
BstSFI CTRYAG 1 cut(s) 3517
BstUI CGCG 4 cut(s) 39, 342, 806, 1125
BstV2I GAAGAC 5 cut(s) 909, 2655, 2801, 4521, 5002
BstX2I RGATCY 6 cut(s) 181, 484, 2488, 3184, 3310, 5716
BstXI CCANNNNNNTGG 2 cut(s) 3597, 5170
BstYI RGATCY 6 cut(s) 181, 484, 2488, 3184, 3310, 5716
BstZ17I GTATAC 2 cut(s) 2400, 4678
Bsu15I ATCGAT 2 cut(s) 322, 4008
Bsu36I CCTNAGG 1 cut(s) 3948
BsuI GTATCC 2 cut(s) 2135, 4292
BsuTUI ATCGAT 2 cut(s) 322, 4008
BtgI CCRYGG 1 cut(s) 5065
BtgZI GCGATG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 2007
BtuMI TCGCGA 1 cut(s) 806
CaiI CAGNNNCTG 4 cut(s) 992, 2450, 4663, 5644
Cfr10I RCCGGY 2 cut(s) 5090, 5514
ClaI ATCGAT 2 cut(s) 322, 4008
CpoI CGGWCCG 1 cut(s) 1883
CseI GACGC 2 cut(s) 3684, 4335
Csp6I GTAC 9 cut(s) 916, 1003, 1336, 1606, 2236, 2284, 4752, 5197, 5759
CspI CGGWCCG 1 cut(s) 1883
CviQI GTAC 9 cut(s) 916, 1003, 1336, 1606, 2236, 2284, 4752, 5197, 5759
DinI GGCGCC 2 cut(s) 1945, 1964
DraIII CACNNNGTG 2 cut(s) 4475, 5769
DriI GACNNNNNGTC 1 cut(s) 3276
EaeI YGGCCR 3 cut(s) 3622, 3717, 5050
Eam1104I CTCTTC 7 cut(s) 611, 782, 855, 2049, 2631, 3050, 3996
Eam1105I GACNNNNNGTC 1 cut(s) 3276
EarI CTCTTC 7 cut(s) 611, 782, 855, 2049, 2631, 3050, 3996
EciI GGCGGA 1 cut(s) 2624
Ecl136II GAGCTC 2 cut(s) 865, 3875
Eco130I CCWWGG 7 cut(s) 966, 1682, 3306, 4549, 5025, 5065, 5445
Eco24I GRGCYC 4 cut(s) 867, 2464, 3877, 4608
Eco31I GGTCTC 1 cut(s) 269
Eco32I GATATC 3 cut(s) 3558, 4078, 5625
Eco47I GGWCC 3 cut(s) 1230, 1883, 4417
Eco53kI GAGCTC 2 cut(s) 865, 3875
Eco57I CTGAAG 7 cut(s) 921, 991, 1197, 3749, 3885, 3908, 5596
Eco81I CCTNAGG 1 cut(s) 3948
Eco88I CYCGRG 3 cut(s) 1910, 3843, 4494
Eco91I GGTNACC 1 cut(s) 4849
EcoICRI GAGCTC 2 cut(s) 865, 3875
EcoO109I RGGNCCY 4 cut(s) 65, 368, 1012, 1230
EcoO65I GGTNACC 1 cut(s) 4849
EcoRI GAATTC 4 cut(s) 3126, 3787, 4262, 5775
EcoRII CCWGG 8 cut(s) 759, 1643, 2049, 2226, 2882, 2946, 4117, 5952
EcoRV GATATC 3 cut(s) 3558, 4078, 5625
EcoT14I CCWWGG 7 cut(s) 966, 1682, 3306, 4549, 5025, 5065, 5445
EcoT22I ATGCAT 3 cut(s) 76, 379, 4428
EcoT38I GRGCYC 4 cut(s) 867, 2464, 3877, 4608
EgeI GGCGCC 2 cut(s) 1945, 1964
EheI GGCGCC 2 cut(s) 1945, 1964
ErhI CCWWGG 7 cut(s) 966, 1682, 3306, 4549, 5025, 5065, 5445
FalI AAGNNNNNCTT 6 cut(s) 2886, 2918, 4321, 4353, 5166, 5198
FaqI GGGAC 7 cut(s) 103, 406, 1216, 1830, 3365, 4610, 4907
FauI CCCGC 4 cut(s) 4386, 4587, 4956, 5156
FauNDI CATATG 2 cut(s) 1407, 3898
FbaI TGATCA 2 cut(s) 1498, 4273
FblI GTMKAC 2 cut(s) 2399, 4677
FriOI GRGCYC 4 cut(s) 867, 2464, 3877, 4608
FspAI RTGCGCAY 1 cut(s) 2153
FspI TGCGCA 1 cut(s) 2153
GsaI CCCAGC 2 cut(s) 5407, 5868
GsuI CTGGAG 6 cut(s) 2033, 2324, 3413, 3431, 3518, 3813
HaeII RGCGCY 3 cut(s) 1947, 1966, 4811
HgaI GACGC 2 cut(s) 3684, 4335
Hin1I GRCGYC 3 cut(s) 1944, 1963, 3695
HincII GTYRAC 4 cut(s) 1718, 3354, 4761, 5632
HindII GTYRAC 4 cut(s) 1718, 3354, 4761, 5632
HindIII AAGCTT 4 cut(s) 2969, 4233, 4372, 5409
HpaI GTTAAC 1 cut(s) 4761
HphI GGTGA 9 cut(s) 1364, 3008, 3839, 4148, 4556, 4664, 4835, 5405, 5599
Hpy99I CGWCG 1 cut(s) 4290
HpyCH4IV ACGT 6 cut(s) 2396, 3403, 4599, 4839, 5733, 5927
HpySE526I ACGT 6 cut(s) 2396, 3403, 4599, 4839, 5733, 5927
Hsp92I GRCGYC 3 cut(s) 1944, 1963, 3695
KasI GGCGCC 2 cut(s) 1943, 1962
Ksp22I TGATCA 2 cut(s) 1498, 4273
KspAI GTTAAC 1 cut(s) 4761
LguI GCTCTTC 2 cut(s) 855, 3996
MaeII ACGT 6 cut(s) 2396, 3403, 4599, 4839, 5733, 5927
MbiI CCGCTC 3 cut(s) 28, 331, 385
MfeI CAATTG 3 cut(s) 707, 4167, 4200
MflI RGATCY 6 cut(s) 181, 484, 2488, 3184, 3310, 5716
MhlI GDGCHC 7 cut(s) 867, 1567, 2464, 3328, 3516, 3877, 4608
MlsI TGGCCA 1 cut(s) 3719
MluNI TGGCCA 1 cut(s) 3719
Mly113I GGCGCC 2 cut(s) 1944, 1963
MlyI GAGTC 6 cut(s) 746, 802, 3638, 4555, 4675, 5485
MmeI TCCRAC 5 cut(s) 1876, 1910, 2224, 5151, 5952
Mox20I TGGCCA 1 cut(s) 3719
Mph1103I ATGCAT 3 cut(s) 76, 379, 4428
MroXI GAANNNNTTC 7 cut(s) 1732, 2320, 2580, 4333, 4659, 5178, 5779
MscI TGGCCA 1 cut(s) 3719
MslI CAYNNNNRTG 3 cut(s) 3440, 4440, 5070
Msp20I TGGCCA 1 cut(s) 3719
MspA1I CMGCKG 3 cut(s) 841, 3371, 4393
MspR9I CCNGG 9 cut(s) 761, 1645, 2051, 2228, 2607, 2884, 2948, 4119, 5954
MunI CAATTG 3 cut(s) 707, 4167, 4200
Mva1269I GAATGC 3 cut(s) 1269, 1388, 1832
MvaI CCWGG 8 cut(s) 761, 1645, 2051, 2228, 2884, 2948, 4119, 5954
MvnI CGCG 4 cut(s) 39, 342, 806, 1125
NarI GGCGCC 2 cut(s) 1944, 1963
NciI CCSGG 1 cut(s) 2607
NcoI CCATGG 1 cut(s) 5065
NdeI CATATG 2 cut(s) 1407, 3898
NheI GCTAGC 2 cut(s) 610, 2843
NmeAIII GCCGAG 1 cut(s) 5917
NmuCI GTSAC 3 cut(s) 1614, 3626, 4558
NruI TCGCGA 1 cut(s) 806
NsbI TGCGCA 1 cut(s) 2153
NsiI ATGCAT 3 cut(s) 76, 379, 4428
NspI RCATGY 6 cut(s) 1069, 2257, 2290, 4319, 4714, 5117
PaeI GCATGC 1 cut(s) 4714
PaeR7I CTCGAG 3 cut(s) 1910, 3843, 4494
PciI ACATGT 2 cut(s) 2286, 4315
PciSI GCTCTTC 2 cut(s) 855, 3996
PcsI WCGNNNNNNNCGW 1 cut(s) 3622
PctI GAATGC 3 cut(s) 1269, 1388, 1832
PdmI GAANNNNTTC 7 cut(s) 1732, 2320, 2580, 4333, 4659, 5178, 5779
PfeI GAWTC 8 cut(s) 198, 501, 2203, 2529, 2888, 3365, 4210, 5484
PflMI CCANNNNNTGG 3 cut(s) 3391, 3715, 5769
PfoI TCCNGGA 3 cut(s) 2049, 2605, 2946
PleI GAGTC 6 cut(s) 745, 802, 3638, 4555, 4674, 5484
PluTI GGCGCC 2 cut(s) 1947, 1966
PpsI GAGTC 6 cut(s) 745, 802, 3638, 4555, 4674, 5484
PpuMI RGGWCCY 1 cut(s) 1230
PscI ACATGT 2 cut(s) 2286, 4315
PshBI ATTAAT 1 cut(s) 3119
PsiI TTATAA 1 cut(s) 5588
Psp124BI GAGCTC 2 cut(s) 867, 3877
Psp1406I AACGTT 1 cut(s) 5927
Psp5II RGGWCCY 1 cut(s) 1230
Psp6I CCWGG 8 cut(s) 759, 1643, 2049, 2226, 2882, 2946, 4117, 5952
PspEI GGTNACC 1 cut(s) 4849
PspFI CCCAGC 2 cut(s) 5403, 5864
PspGI CCWGG 8 cut(s) 759, 1643, 2049, 2226, 2882, 2946, 4117, 5952
PspPPI RGGWCCY 1 cut(s) 1230
PspXI VCTCGAGB 1 cut(s) 3843
PstNI CAGNNNCTG 4 cut(s) 992, 2450, 4663, 5644
PsuI RGATCY 6 cut(s) 181, 484, 2488, 3184, 3310, 5716
PvuII CAGCTG 2 cut(s) 841, 3371
RruI TCGCGA 1 cut(s) 806
RsaI GTAC 9 cut(s) 917, 1004, 1337, 1607, 2237, 2285, 4753, 5198, 5760
RsaNI GTAC 9 cut(s) 916, 1003, 1336, 1606, 2236, 2284, 4752, 5197, 5759
RseI CAYNNNNRTG 3 cut(s) 3440, 4440, 5070
Rsr2I CGGWCCG 1 cut(s) 1883
RsrII CGGWCCG 1 cut(s) 1883
SacI GAGCTC 2 cut(s) 867, 3877
SapI GCTCTTC 2 cut(s) 855, 3996
ScaI AGTACT 2 cut(s) 1004, 1607
SchI GAGTC 6 cut(s) 746, 802, 3638, 4555, 4675, 5485
ScrFI CCNGG 9 cut(s) 761, 1645, 2051, 2228, 2607, 2884, 2948, 4119, 5954
SduI GDGCHC 7 cut(s) 867, 1567, 2464, 3328, 3516, 3877, 4608
SfcI CTRYAG 1 cut(s) 3517
SfoI GGCGCC 2 cut(s) 1945, 1964
Sfr274I CTCGAG 3 cut(s) 1910, 3843, 4494
SinI GGWCC 3 cut(s) 1230, 1883, 4417
SlaI CTCGAG 3 cut(s) 1910, 3843, 4494
SmiMI CAYNNNNRTG 3 cut(s) 3440, 4440, 5070
SmlI CTYRAG 7 cut(s) 6, 309, 866, 1910, 3677, 3843, 4494
SmoI CTYRAG 7 cut(s) 6, 309, 866, 1910, 3677, 3843, 4494
SphI GCATGC 1 cut(s) 4714
SspDI GGCGCC 2 cut(s) 1943, 1962
SspI AATATT 3 cut(s) 922, 1696, 2988
SstI GAGCTC 2 cut(s) 867, 3877
StyD4I CCNGG 9 cut(s) 759, 1643, 2049, 2226, 2605, 2882, 2946, 4117, 5952
StyI CCWWGG 7 cut(s) 966, 1682, 3306, 4549, 5025, 5065, 5445
TaiI ACGT 6 cut(s) 2399, 3406, 4602, 4842, 5736, 5930
TaqII GACCGA 1 cut(s) 1871
TatI WGTACW 5 cut(s) 1002, 1335, 1605, 2283, 4751
TauI GCSGC 3 cut(s) 388, 893, 3836
TfiI GAWTC 8 cut(s) 198, 501, 2203, 2529, 2888, 3365, 4210, 5484
TseFI GTSAC 3 cut(s) 1614, 3626, 4558
Tsp45I GTSAC 3 cut(s) 1614, 3626, 4558
TspGWI ACGGA 7 cut(s) 3283, 3287, 3452, 3631, 4274, 4409, 5828
Van91I CCANNNNNTGG 3 cut(s) 3391, 3715, 5769
VpaK11BI GGWCC 3 cut(s) 1230, 1883, 4417
VspI ATTAAT 1 cut(s) 3119
XbaI TCTAGA 5 cut(s) 194, 235, 497, 538, 2918
XceI RCATGY 6 cut(s) 1069, 2257, 2290, 4319, 4714, 5117
XcmI CCANNNNNNNNNTGG 4 cut(s) 209, 1575, 4274, 5263
XhoI CTCGAG 3 cut(s) 1910, 3843, 4494
XmiI GTMKAC 2 cut(s) 2399, 4677
XmnI GAANNNNTTC 7 cut(s) 1732, 2320, 2580, 4333, 4659, 5178, 5779
ZrmI AGTACT 2 cut(s) 1004, 1607
Zsp2I ATGCAT 3 cut(s) 76, 379, 4428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.