FvH4_2g06330

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000561
Physical Location & Seq
Reverse (-)
5261681 .. 5264312
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06330.t2

Sequence Viewer

Length: 1128 bp
ATGATTTGTATCAGAGAATCCTTTTCGACAAGTAATCGTCCCTATCATGGTTCCACAAACTGGACAAGCCGCAGTGACGTGGCAACTGTCCGTCTGAGAAGGACGGAGAAAACGTCTGAGTATTACCGAGAAGAAGAAGAAGAAGAAGAAGAAGACGAGGAGGTACAGGAGGACAGCATGGACATTTCCCCGCTCTCTGTCCCCAGACAACAATTTGACAATCTGACCCTTGATTCACCCTCGGTACAGCAGAGGAAGCGCTCTAAGACCCGCATAAGTCGTTTTAGTCCAACCCAACTTGTCAAATTCCACAATTTGACCCTCAATACTCCAAACTCGGAACAGAGGCAGCCATTGGAGGAAGCTAACAAATATGGGTTCAAAACTCCCAAACGGAAAAGACATTTGAGTCCAAGCCTTAACACCAACCCAGAAATATCTAGTGATCCTCAGAAGCATGGAGGCTGCAAACGGAGTAGAACAAACAGGTCCATGTCCTGGCATTTTGGCCATCCTGAAGAGTCTCCTGACAGAAGCCAAGTTAAAGAAATTCCAGAAAATCTAGAAGCTGATGGATTCCCCTTCTTTATCAAGTCCATGATACGATCAAATATTGCTGTTTGCTTTATGTTGACACTGCCTAGGAAATTCTGCCACTTGCATTTACCTTTGTATGGCACAGCAACTATCACATTGGAAGATAAAGAAGGGAAAGAGTTTGAAACAAATTTTCTTGCCGACAAAAGGGCGCTTAGTGGTGGATGGAAAGGGTTTTGTCAAGCAAAAGACTTGCGTCTTGATGATGTTCTAGTTTTCCAGTTGGTTAGCCCTTTGAAGTTTAAGGTGCACAAATTGAGACCAGACTATGATCTAAATGAAGAAGATGTAGATGCAGCTTTTGTCCTCTTGAAAATGAGTACCTCTGAACAAACCTTGTTAGAGGAAGACCCTCCGACGCCTCATCCAAGGGATGTTTTCCACGGCAATATTCGAGAGATATGTCTAATACCAGATTTAAATGTTGAGCTTGTAGATGATGATGAGACTGAGGATCGGATTACAAAGACGATAAATTTGGCTCCAATTCTACAGGGTGGTGGATTTGATCTAGCCAGGACAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

376

Amino Acids

43.19

Weight (kDa)

5.59

Isoelectric Point (pI)

68.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 135 - 222 4.5e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 408
AccB7I CCANNNNNTGG 2 cut(s) 60, 498
AccBSI CCGCTC 1 cut(s) 193
AciI CCGC 3 cut(s) 70, 191, 271
AclWI GGATC 2 cut(s) 440, 1059
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 5 cut(s) 305, 549, 647, 727, 1072
AcuI CTGAAG 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 956
AfaI GTAC 3 cut(s) 165, 246, 919
AfeI AGCGCT 1 cut(s) 260
AfiI CCNNNNNNNGG 5 cut(s) 47, 60, 498, 674, 744
AgsI TTSAA 4 cut(s) 382, 722, 835, 910
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 497, 1112
AluBI AGCT 5 cut(s) 365, 569, 896, 1027, 1122
AluI AGCT 5 cut(s) 365, 569, 896, 1027, 1122
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 3 cut(s) 528, 850, 1037
Alw44I GTGCAC 1 cut(s) 845
AlwI GGATC 2 cut(s) 440, 1059
Aor51HI AGCGCT 1 cut(s) 260
AoxI GGCC 1 cut(s) 508
ApaLI GTGCAC 1 cut(s) 845
ApeKI GCWGC 4 cut(s) 349, 465, 893, 1122
ApoI RAATTY 5 cut(s) 305, 549, 647, 727, 1072
ArsI GACNNNNNNTTYG 2 cut(s) 1057, 1089
AspA2I CCTAGG 1 cut(s) 641
AspLEI GCGC 2 cut(s) 261, 751
AspS9I GGNCC 1 cut(s) 489
AsuHPI GGTGA 1 cut(s) 228
AvaII GGWCC 1 cut(s) 489
AvrII CCTAGG 1 cut(s) 641
BaeGI GKGCMC 1 cut(s) 849
BalI TGGCCA 1 cut(s) 510
BbsI GAAGAC 2 cut(s) 159, 951
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 4 cut(s) 361, 452, 905, 1109
BccI CCATC 3 cut(s) 519, 566, 756
BceAI ACGGC 1 cut(s) 997
BciT130I CCWGG 2 cut(s) 499, 1114
BcoDI GTCTC 3 cut(s) 528, 850, 1037
BfaI CTAG 5 cut(s) 441, 563, 642, 809, 1109
BfmI CTRYAG 1 cut(s) 1088
BfoI RGCGCY 2 cut(s) 262, 752
BisI GCNGC 5 cut(s) 70, 350, 466, 894, 1123
BlnI CCTAGG 1 cut(s) 641
BlsI GCNGC 5 cut(s) 71, 351, 467, 895, 1124
Bme1390I CCNGG 2 cut(s) 499, 1114
Bme18I GGWCC 1 cut(s) 489
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 489
BmiI GGNNCC 2 cut(s) 52, 1080
BmrFI CCNGG 2 cut(s) 499, 1114
BmsI GCATC 1 cut(s) 880
BoxI GACNNNNGTC 1 cut(s) 792
BpiI GAAGAC 2 cut(s) 159, 951
BsaBI GATNNNNATC 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 956
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 4 cut(s) 240, 641, 965, 979
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 60, 498, 674, 744
Bse1I ACTGG 2 cut(s) 65, 817
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 2 cut(s) 499, 1114
BseDI CCNNGG 4 cut(s) 240, 641, 965, 979
BseGI GGATG 4 cut(s) 511, 767, 961, 976
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 5 cut(s) 47, 60, 498, 674, 744
BseMII CTCAG 4 cut(s) 86, 108, 464, 1038
BseNI ACTGG 2 cut(s) 65, 817
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 849
BseXI GCAGC 4 cut(s) 361, 452, 905, 1109
BshFI GGCC 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 849
BslFI GGGAC 2 cut(s) 24, 185
BslI CCNNNNNNNGG 5 cut(s) 47, 60, 498, 674, 744
BsmAI GTCTC 3 cut(s) 528, 850, 1037
BsmFI GGGAC 2 cut(s) 24, 185
BsnI GGCC 1 cut(s) 510
Bso31I GGTCTC 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 849
Bsp143I GATC 5 cut(s) 445, 605, 868, 1051, 1105
BspACI CCGC 3 cut(s) 70, 191, 271
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 4 cut(s) 87, 109, 463, 1039
BspLI GGNNCC 2 cut(s) 52, 1080
BspPI GGATC 2 cut(s) 440, 1059
BspTNI GGTCTC 1 cut(s) 850
BsrBI CCGCTC 1 cut(s) 193
BsrI ACTGG 2 cut(s) 65, 817
BssECI CCNNGG 4 cut(s) 240, 641, 965, 979
BssMI GATC 5 cut(s) 445, 605, 868, 1051, 1105
BssNI GRCGYC 1 cut(s) 956
BssT1I CCWWGG 2 cut(s) 641, 965
Bst2UI CCWGG 2 cut(s) 499, 1114
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 1 cut(s) 513
BstACI GRCGYC 1 cut(s) 956
BstDEI CTNAG 6 cut(s) 95, 117, 264, 450, 752, 1047
BstDSI CCRYGG 1 cut(s) 979
BstF5I GGATG 4 cut(s) 511, 767, 961, 976
BstH2I RGCGCY 2 cut(s) 262, 752
BstHHI GCGC 2 cut(s) 261, 751
BstKTI GATC 5 cut(s) 448, 608, 871, 1054, 1108
BstMAI GTCTC 3 cut(s) 528, 850, 1037
BstMBI GATC 5 cut(s) 445, 605, 868, 1051, 1105
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNI CCWGG 2 cut(s) 499, 1114
BstPAI GACNNNNGTC 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 497, 1112
BstSFI CTRYAG 1 cut(s) 1088
BstSLI GKGCMC 1 cut(s) 849
BstV1I GCAGC 4 cut(s) 361, 452, 905, 1109
BstV2I GAAGAC 2 cut(s) 159, 951
BsuRI GGCC 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 979
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 4 cut(s) 511, 767, 961, 976
BtsI GCAGTG 2 cut(s) 79, 635
BtsIMutI CAGTG 2 cut(s) 79, 635
CfoI GCGC 2 cut(s) 261, 751
Cfr13I GGNCC 1 cut(s) 489
CseI GACGC 2 cut(s) 782, 964
Csp6I GTAC 3 cut(s) 164, 245, 918
CviAII CATG 5 cut(s) 47, 178, 458, 493, 598
CviQI GTAC 3 cut(s) 164, 245, 918
DdeI CTNAG 6 cut(s) 95, 117, 264, 450, 752, 1047
DpnI GATC 5 cut(s) 447, 607, 870, 1053, 1107
DpnII GATC 5 cut(s) 445, 605, 868, 1051, 1105
DraI TTTAAA 1 cut(s) 1017
DrdI GACNNNNNNGTC 1 cut(s) 408
DseDI GACNNNNNNGTC 1 cut(s) 408
EaeI YGGCCR 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco130I CCWWGG 2 cut(s) 641, 965
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 1 cut(s) 489
Eco47III AGCGCT 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 537
EcoRII CCWGG 2 cut(s) 497, 1112
EcoT14I CCWWGG 2 cut(s) 641, 965
ErhI CCWWGG 2 cut(s) 641, 965
FaeI CATG 5 cut(s) 50, 181, 461, 496, 601
FaqI GGGAC 2 cut(s) 24, 185
FatI CATG 5 cut(s) 46, 177, 457, 492, 597
FauI CCCGC 2 cut(s) 198, 278
Fnu4HI GCNGC 5 cut(s) 70, 350, 466, 894, 1123
FokI GGATG 4 cut(s) 498, 774, 948, 983
Fsp4HI GCNGC 5 cut(s) 70, 350, 466, 894, 1123
FspBI CTAG 5 cut(s) 441, 563, 642, 809, 1109
GlaI GCGC 2 cut(s) 260, 750
GluI GCNGC 5 cut(s) 70, 350, 466, 894, 1123
HaeII RGCGCY 2 cut(s) 262, 752
HaeIII GGCC 1 cut(s) 510
HgaI GACGC 2 cut(s) 782, 964
HhaI GCGC 2 cut(s) 261, 751
Hin1I GRCGYC 1 cut(s) 956
Hin1II CATG 5 cut(s) 50, 181, 461, 496, 601
Hin6I GCGC 2 cut(s) 259, 749
HinP1I GCGC 2 cut(s) 259, 749
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HinfI GANTC 5 cut(s) 17, 233, 409, 521, 576
HphI GGTGA 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 633, 847
Hpy188I TCNGA 9 cut(s) 14, 96, 118, 225, 340, 453, 925, 954, 1056
Hpy188III TCNNGA 7 cut(s) 515, 527, 554, 563, 797, 907, 992
Hpy8I GTNNAC 2 cut(s) 633, 847
Hpy99I CGWCG 1 cut(s) 958
HpyAV CCTTC 3 cut(s) 93, 592, 701
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 78, 113
HpyCH4V TGCA 4 cut(s) 468, 661, 847, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 6 cut(s) 95, 117, 264, 450, 752, 1047
HpySE526I ACGT 2 cut(s) 78, 113
Hsp92I GRCGYC 1 cut(s) 956
Hsp92II CATG 5 cut(s) 50, 181, 461, 496, 601
HspAI GCGC 2 cut(s) 259, 749
Kzo9I GATC 5 cut(s) 445, 605, 868, 1051, 1105
LmnI GCTCC 1 cut(s) 1084
Lsp1109I GCAGC 4 cut(s) 361, 452, 905, 1109
LweI GCATC 1 cut(s) 880
MaeI CTAG 5 cut(s) 441, 563, 642, 809, 1109
MaeII ACGT 2 cut(s) 78, 113
MaeIII GTNAC 1 cut(s) 74
MalI GATC 5 cut(s) 447, 607, 870, 1053, 1107
MbiI CCGCTC 1 cut(s) 193
MboI GATC 5 cut(s) 445, 605, 868, 1051, 1105
MhlI GDGCHC 1 cut(s) 849
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 9 cut(s) 212, 305, 313, 549, 647, 727, 851, 1072, 1083
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 2 cut(s) 418, 530
MmeI TCCRAC 2 cut(s) 314, 977
Mox20I TGGCCA 1 cut(s) 510
MscI TGGCCA 1 cut(s) 510
MseI TTAA 4 cut(s) 420, 543, 840, 1016
Msp20I TGGCCA 1 cut(s) 510
MspR9I CCNGG 2 cut(s) 499, 1114
MvaI CCWGG 2 cut(s) 499, 1114
MwoI GCNNNNNNNGC 1 cut(s) 256
NdeII GATC 5 cut(s) 445, 605, 868, 1051, 1105
NlaIII CATG 5 cut(s) 50, 181, 461, 496, 601
NlaIV GGNNCC 2 cut(s) 52, 1080
NmuCI GTSAC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PfeI GAWTC 3 cut(s) 17, 233, 576
PflMI CCANNNNNTGG 2 cut(s) 60, 498
PkrI GCNGC 5 cut(s) 71, 351, 467, 895, 1124
PleI GAGTC 2 cut(s) 417, 529
PpsI GAGTC 2 cut(s) 417, 529
PshAI GACNNNNGTC 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 497, 1112
PspGI CCWGG 2 cut(s) 497, 1112
PspN4I GGNNCC 2 cut(s) 52, 1080
PspPI GGNCC 1 cut(s) 489
RsaI GTAC 3 cut(s) 165, 246, 919
RsaNI GTAC 3 cut(s) 164, 245, 918
SaqAI TTAA 4 cut(s) 420, 543, 840, 1016
SatI GCNGC 5 cut(s) 70, 350, 466, 894, 1123
Sau3AI GATC 5 cut(s) 445, 605, 868, 1051, 1105
Sau96I GGNCC 1 cut(s) 489
SchI GAGTC 2 cut(s) 418, 530
ScrFI CCNGG 2 cut(s) 499, 1114
SduI GDGCHC 1 cut(s) 849
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 1088
SinI GGWCC 1 cut(s) 489
SmiI ATTTAAAT 1 cut(s) 1017
Sse9I AATT 9 cut(s) 212, 305, 313, 549, 647, 727, 851, 1072, 1083
SsiI CCGC 3 cut(s) 70, 191, 271
SspI AATATT 2 cut(s) 613, 988
SspMI CTAG 5 cut(s) 441, 563, 642, 809, 1109
StyD4I CCNGG 2 cut(s) 497, 1112
StyI CCWWGG 2 cut(s) 641, 965
SwaI ATTTAAAT 1 cut(s) 1017
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 2 cut(s) 81, 116
TaqI TCGA 2 cut(s) 26, 991
TasI AATT 9 cut(s) 212, 305, 313, 549, 647, 727, 851, 1072, 1083
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 3 cut(s) 17, 233, 576
Tru1I TTAA 4 cut(s) 420, 543, 840, 1016
Tru9I TTAA 4 cut(s) 420, 543, 840, 1016
TscAI CASTG 2 cut(s) 79, 642
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 4 cut(s) 349, 465, 893, 1122
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 4 cut(s) 80, 119, 409, 487
TspRI CASTG 2 cut(s) 79, 642
Van91I CCANNNNNTGG 2 cut(s) 60, 498
VneI GTGCAC 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 489
XapI RAATTY 5 cut(s) 305, 549, 647, 727, 1072
XbaI TCTAGA 1 cut(s) 562
XmaJI CCTAGG 1 cut(s) 641
XspI CTAG 5 cut(s) 441, 563, 642, 809, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.