FvH4_2g06320

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000561
Physical Location & Seq
Reverse (-)
5251498 .. 5254191
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06320.t8

Sequence Viewer

Length: 816 bp
ATGAATCTGGGGCAGGAGAATTTCTTCCAGTATTTCCAGGAAGACGAAGAGAATCCCACCACCATCCCTGACCTCTCTGTCCCTTCTACCACTACTACTACAACTCCATTTGACGATTTCACCCTTCATTCCCACACCTCGCCGCCTTCGGTAGAGCAGCACTGCCAAAATGGGTTCAAGCCTTGGAAGTGGAAAAGTCCCTTGAGTCCAATTCCTAAGCGAAAAAATGCTGGGGATCCCCTGAAGCATCGAGGCTATAAGCGCAAGATGACAAAGTCCAACTATCATGAAAAGTCTTCTGTCAGAAACCAACTGCAAGATATCCTGGCAAATCTAGGACCTGAGTGCCCCTTCTTTTTGAAGTCTATGATACATTGTAATGTTGTACATGGCTTTAGTTTGACGTTGCCAAAGCAATTCTGCTATTTGTATTTGCCATTCCATGATACAACTATAACCTTGGAAGATGAAAGAGGTGAAGAATATAGAACAGTATATCTTGGGGACAAGAGGAGATTGAGTGGTGGGTGGAGAGCGTTTTGTGTCGCAATGAACTTGGTTCTTGGTGACCTTTTAGTTTTCCATTTGGTCGGTCCTTTGAAGTTTAAGGTGTACAAAGTGGAACCGGATAATTTAAATGAAGTGGATGCAGCTTTTATCCTCTTGAAATTGAGTCTCTCCACACTATTTGCTAAAATGTTAACGGTAAATGACGGTGCAGAAGAGTACCATCCAACGTCTCCTCCAAAAGATATTTGCCAAGAAAATATTCGAACGACAAGTCTGGAAATAGATGCCATTTTTTTGAGCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

272

Amino Acids

31.0

Weight (kDa)

6.36

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 30 - 113 1e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AciI CCGC 1 cut(s) 143
AclWI GGATC 2 cut(s) 230, 243
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 3 cut(s) 387, 614, 728
AgsI TTSAA 4 cut(s) 178, 361, 601, 667
AjnI CCWGG 2 cut(s) 36, 324
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 1 cut(s) 653
AluI AGCT 1 cut(s) 653
Alw26I GTCTC 2 cut(s) 680, 744
AlwI GGATC 2 cut(s) 230, 243
ApeKI GCWGC 2 cut(s) 157, 650
ApoI RAATTY 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
Asp700I GAANNNNTTC 2 cut(s) 23, 768
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 338, 593
AsuHPI GGTGA 3 cut(s) 112, 488, 578
AsuII TTCGAA 1 cut(s) 772
AvaII GGWCC 2 cut(s) 338, 593
BaeGI GKGCMC 1 cut(s) 350
BamHI GGATCC 1 cut(s) 235
BbsI GAAGAC 2 cut(s) 48, 288
BbvI GCAGC 2 cut(s) 169, 662
BccI CCATC 2 cut(s) 71, 738
BciT130I CCWGG 2 cut(s) 38, 326
BcoDI GTCTC 2 cut(s) 680, 744
BfaI CTAG 1 cut(s) 335
BisI GCNGC 3 cut(s) 143, 158, 651
BlsI GCNGC 3 cut(s) 144, 159, 652
Bme1390I CCNGG 2 cut(s) 38, 326
Bme18I GGWCC 2 cut(s) 338, 593
BmgT120I GGNCC 2 cut(s) 338, 593
BmiI GGNNCC 2 cut(s) 237, 624
BmrFI CCNGG 2 cut(s) 38, 326
BmsI GCATC 3 cut(s) 256, 637, 784
BpiI GAAGAC 2 cut(s) 48, 288
Bpu10I CCTNAGC 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 182, 459
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 6 cut(s) 88, 118, 505, 535, 727, 757
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 555
BseBI CCWGG 2 cut(s) 38, 326
BseDI CCNNGG 2 cut(s) 182, 459
BseGI GGATG 3 cut(s) 63, 652, 730
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 2 cut(s) 526, 732
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 2 cut(s) 169, 662
BseYI CCCAGC 1 cut(s) 230
BsgI GTGCAG 1 cut(s) 738
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 3 cut(s) 65, 183, 518
BsmAI GTCTC 2 cut(s) 680, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 3 cut(s) 65, 183, 518
Bsp119I TTCGAA 1 cut(s) 772
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 2 cut(s) 385, 612
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 237, 624
BspPI GGATC 2 cut(s) 230, 243
BspT104I TTCGAA 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 555
BsrGI TGTACA 2 cut(s) 385, 612
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 182, 459
BssMI GATC 1 cut(s) 235
BssT1I CCWWGG 2 cut(s) 182, 459
Bst2UI CCWGG 2 cut(s) 38, 326
Bst4CI ACNGT 3 cut(s) 493, 706, 716
Bst6I CTCTTC 2 cut(s) 42, 717
BstAUI TGTACA 2 cut(s) 385, 612
BstBI TTCGAA 1 cut(s) 772
BstDEI CTNAG 2 cut(s) 216, 342
BstEII GGTNACC 1 cut(s) 566
BstF5I GGATG 3 cut(s) 63, 652, 730
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 2 cut(s) 680, 744
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 38, 326
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 36, 324
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 2 cut(s) 169, 662
BstV2I GAAGAC 2 cut(s) 48, 288
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BtsCI GGATG 3 cut(s) 63, 652, 730
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 160
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 2 cut(s) 338, 593
Csp6I GTAC 3 cut(s) 386, 613, 727
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviAII CATG 3 cut(s) 287, 389, 443
CviJI RGCY 4 cut(s) 181, 255, 393, 653
CviKI_1 RGCY 4 cut(s) 181, 255, 393, 653
CviQI GTAC 3 cut(s) 386, 613, 727
DdeI CTNAG 2 cut(s) 216, 342
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DraI TTTAAA 1 cut(s) 636
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 2 cut(s) 42, 717
EarI CTCTTC 2 cut(s) 42, 717
Eco130I CCWWGG 2 cut(s) 182, 459
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 338, 593
Eco57I CTGAAG 1 cut(s) 263
Eco91I GGTNACC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 338
EcoO65I GGTNACC 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 36, 324
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 182, 459
ErhI CCWWGG 2 cut(s) 182, 459
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 3 cut(s) 290, 392, 446
FaiI YATR 8 cut(s) 258, 288, 368, 390, 444, 455, 486, 496
FaqI GGGAC 3 cut(s) 65, 183, 518
FatI CATG 3 cut(s) 286, 388, 442
Fnu4HI GCNGC 3 cut(s) 143, 158, 651
FokI GGATG 3 cut(s) 50, 659, 717
Fsp4HI GCNGC 3 cut(s) 143, 158, 651
FspBI CTAG 1 cut(s) 335
GlaI GCGC 1 cut(s) 263
GluI GCNGC 3 cut(s) 143, 158, 651
GsaI CCCAGC 1 cut(s) 234
HapII CCGG 1 cut(s) 626
HhaI GCGC 1 cut(s) 264
Hin1II CATG 3 cut(s) 290, 392, 446
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HinfI GANTC 4 cut(s) 4, 52, 205, 673
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 1 cut(s) 626
HphI GGTGA 3 cut(s) 112, 488, 578
Hpy166II GTNNAC 2 cut(s) 613, 702
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 3 cut(s) 287, 664, 785
Hpy8I GTNNAC 2 cut(s) 613, 702
HpyAV CCTTC 4 cut(s) 93, 134, 156, 361
HpyCH4III ACNGT 3 cut(s) 493, 706, 716
HpyCH4IV ACGT 2 cut(s) 404, 737
HpyCH4V TGCA 3 cut(s) 316, 650, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 216, 342
HpySE526I ACGT 2 cut(s) 404, 737
Hsp92II CATG 3 cut(s) 290, 392, 446
HspAI GCGC 1 cut(s) 262
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 169, 662
LweI GCATC 3 cut(s) 256, 637, 784
MaeI CTAG 1 cut(s) 335
MaeII ACGT 2 cut(s) 404, 737
MaeIII GTNAC 1 cut(s) 566
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 7 cut(s) 16, 53, 59, 288, 476, 491, 734
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 5 cut(s) 19, 210, 416, 631, 668
MlyI GAGTC 2 cut(s) 214, 682
MmeI TCCRAC 2 cut(s) 303, 758
MnlI CCTC 7 cut(s) 83, 148, 245, 467, 504, 671, 753
MroXI GAANNNNTTC 2 cut(s) 23, 768
MseI TTAA 3 cut(s) 606, 635, 701
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 2 cut(s) 38, 326
MvaI CCWGG 2 cut(s) 38, 326
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 1 cut(s) 235
NlaIII CATG 3 cut(s) 290, 392, 446
NlaIV GGNNCC 2 cut(s) 237, 624
NmuCI GTSAC 1 cut(s) 566
NspV TTCGAA 1 cut(s) 772
PagI TCATGA 1 cut(s) 286
PdmI GAANNNNTTC 2 cut(s) 23, 768
PfeI GAWTC 2 cut(s) 4, 52
PflFI GACNNNGTC 1 cut(s) 274
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 3 cut(s) 144, 159, 652
PleI GAGTC 2 cut(s) 213, 681
PpsI GAGTC 2 cut(s) 213, 681
PpuMI RGGWCCY 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 36, 324
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 36, 324
PspN4I GGNNCC 2 cut(s) 237, 624
PspPI GGNCC 2 cut(s) 338, 593
PspPPI RGGWCCY 1 cut(s) 338
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 274
RsaI GTAC 3 cut(s) 387, 614, 728
RsaNI GTAC 3 cut(s) 386, 613, 727
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 606, 635, 701
SatI GCNGC 3 cut(s) 143, 158, 651
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 2 cut(s) 338, 593
SchI GAGTC 2 cut(s) 214, 682
ScrFI CCNGG 2 cut(s) 38, 326
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 3 cut(s) 256, 637, 784
SfuI TTCGAA 1 cut(s) 772
SinI GGWCC 2 cut(s) 338, 593
SmiI ATTTAAAT 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 5 cut(s) 19, 210, 416, 631, 668
SsiI CCGC 1 cut(s) 143
SspI AATATT 1 cut(s) 769
SspMI CTAG 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 36, 324
StyI CCWWGG 2 cut(s) 182, 459
SwaI ATTTAAAT 1 cut(s) 636
TaaI ACNGT 3 cut(s) 493, 706, 716
TaiI ACGT 2 cut(s) 407, 740
TaqI TCGA 2 cut(s) 250, 772
TaqII GACCGA 1 cut(s) 581
TasI AATT 5 cut(s) 19, 210, 416, 631, 668
TatI WGTACW 2 cut(s) 385, 612
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 4, 52
Tru1I TTAA 3 cut(s) 606, 635, 701
Tru9I TTAA 3 cut(s) 606, 635, 701
TscAI CASTG 1 cut(s) 167
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 2 cut(s) 157, 650
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 6 cut(s) 17, 116, 303, 483, 566, 654
TspRI CASTG 1 cut(s) 167
Tth111I GACNNNGTC 1 cut(s) 274
VpaK11BI GGWCC 2 cut(s) 338, 593
XapI RAATTY 1 cut(s) 19
XmnI GAANNNNTTC 2 cut(s) 23, 768
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.