Prupe.8G122500_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000561
Physical Location & Seq
Forward (+)
14815603 .. 14818803
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G122500.2

Sequence Viewer

Length: 1353 bp
ATGGCGATGACCCACGAGTGGAAACACAGTAAAAACTTCATGCTCACCATGAATGTTAAGGAAGAAGAACTCTCTGACCCTGAGTATGTTCCACAAGAAAATGAAGAAGAAGAAGAAGAAGAAGAAGAAGACTATTTGAGTGTTTCCCAGTTTTCTCTCCGCAGCCGCCGAAGAACCCATCATTCTTCCCTTTCGGTAAAGCCGAGGGAGCCATGGAAGACCAAGAAAAATGTGCTCAACACTCGAAGAAGTCCTTTAAGTCCGACCCATATGACCAAGCGAAAAGTTCCTGGGGATCCTCGGAAGCATGGAGGCTGTAAACGGAAGAGAACCATGTTTGATAATTTTAACGAACATGAAAAGTCTTCTGCCAGCACCCAAGTTGAGGAGATCCAGGCAATTCTTGGAGCTGAATACCCCATCGTTATTAAGTCTATGATACATTCAAATGTTTCCTCTTGGAATTGTCTGACACTGCCGAAGCAATTCTGCCATTTGCATTTACCATTGCATAATACAACATTAACCCTGGAAGTCGAAAGTGGGGAAAAATATAGAATTATTTTTCTTGCCGGCAAGAGGATACTAAGTGCTGGATGGAAAAAGTTTTGTACTGCACACAAGTTACGAGTTGGTGATCTCTTAGTTTTACGTTTGGTCAGGCCTTTGAAGTTTAAGGTGTACATAATGGGAAGACATAATCTAGCTGAAGTAGATGCAGCTTTTAGCCTCTTGAAATTGAATGCCTGCGCCAAACATGAAGTGCTTGAGGAAAACCATCCAAAGCCTCTTTCAAGTGATGTTTGCCAAGGCAACATTCGAAAGACAAGTCTGATGTTAGATACTAAGCCTGAGCTTATAGATAATCAAACTGAGGAAGACAATGAGGAATTTGGCTCCAAATTTCCAGGAGGCATTAGATATCCAGTACCAGCAGCTGATTTCGAAGATGTTATAAGTATTGAGAATTTCACCATTCTTGTGAATGGTGTGACTATAGATTCTTGGCTTTCCAAACAGCTCAGGACTAACTATTACGATCTATGCTGCTCACGAGGTTCATTTCTGCATGATCATCTCCTCAAAAGTATTAACAGTAAGTTGGCTGCTGAAATAATAACTGAAACTACTAACATTGCTAATGCCATTAGAGCTCGCAAGCTGTCCGCCTCTCATACTGATTACGAAATGTGGGAGAAGACTTTAAATGCCTTTGAGCTGTTGGGTATGGATGTTGGATTCTTAAATGCACAACTGGAGCGGCTTCTGAGTCCCTCCTTCAGATCAGAAGAGGCTGTGGAATTAAAGAGGATAGAAGCTAAAGTTGAGCAAATTTGTGCAGGGGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

451

Amino Acids

51.55

Weight (kDa)

7.02

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 956
AccBSI CCGCTC 1 cut(s) 1261
AciI CCGC 4 cut(s) 160, 166, 1167, 1261
AclWI GGATC 3 cut(s) 290, 303, 385
AcsI RAATTY 4 cut(s) 890, 902, 967, 1332
AcuI CTGAAG 2 cut(s) 729, 1264
AfaI GTAC 3 cut(s) 613, 683, 930
AfiI CCNNNNNNNGG 3 cut(s) 18, 385, 579
AgsI TTSAA 5 cut(s) 447, 670, 736, 742, 795
AjnI CCWGG 4 cut(s) 289, 393, 528, 907
AjuI GAANNNNNNNTTGG 4 cut(s) 775, 801, 807, 833
Alw21I GWGCWC 2 cut(s) 237, 1156
AlwI GGATC 3 cut(s) 290, 303, 385
AlwNI CAGNNNCTG 1 cut(s) 938
AoxI GGCC 1 cut(s) 662
ApeKI GCWGC 5 cut(s) 162, 719, 935, 1047, 1106
ApoI RAATTY 4 cut(s) 890, 902, 967, 1332
ArsI GACNNNNNNTTYG 2 cut(s) 814, 846
Asp700I GAANNNNTTC 1 cut(s) 485
AspLEI GCGC 1 cut(s) 752
AsuHPI GGTGA 3 cut(s) 37, 647, 964
AsuII TTCGAA 2 cut(s) 820, 945
BamHI GGATCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 1156
BauI CACGAG 2 cut(s) 14, 1053
BbsI GAAGAC 6 cut(s) 135, 224, 357, 700, 885, 1205
Bbv12I GWGCWC 2 cut(s) 237, 1156
BbvI GCAGC 5 cut(s) 174, 731, 947, 1034, 1093
BccI CCATC 4 cut(s) 186, 428, 591, 786
BciT130I CCWGG 4 cut(s) 291, 395, 530, 909
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 1072
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 996
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 7 cut(s) 163, 166, 720, 936, 1048, 1107, 1262
BlsI GCNGC 7 cut(s) 164, 167, 721, 937, 1049, 1108, 1263
Bme1390I CCNGG 4 cut(s) 291, 395, 530, 909
BmiI GGNNCC 3 cut(s) 210, 297, 898
BmrFI CCNGG 4 cut(s) 291, 395, 530, 909
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 706
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 6 cut(s) 135, 224, 357, 700, 885, 1205
BpmI CTGGAG 1 cut(s) 1277
Bpu10I CCTNAGC 2 cut(s) 852, 1022
Bpu14I TTCGAA 2 cut(s) 820, 945
BpuEI CTTGAG 1 cut(s) 788
BsaJI CCNNGG 6 cut(s) 203, 212, 290, 299, 528, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 385, 579
Bse118I RCCGGY 1 cut(s) 572
Bse1I ACTGG 3 cut(s) 148, 926, 1260
Bse3DI GCAATG 2 cut(s) 506, 1134
BseBI CCWGG 4 cut(s) 291, 395, 530, 909
BseDI CCNNGG 6 cut(s) 203, 212, 290, 299, 528, 808
BseGI GGATG 3 cut(s) 602, 778, 1237
BseLI CCNNNNNNNGG 3 cut(s) 18, 385, 579
BseMI GCAATG 2 cut(s) 506, 1134
BseMII CTCAG 5 cut(s) 72, 843, 864, 1036, 1259
BseNI ACTGG 3 cut(s) 148, 926, 1260
BseRI GAGGAG 2 cut(s) 401, 1070
BseXI GCAGC 5 cut(s) 174, 731, 947, 1034, 1093
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 237, 1156
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 3 cut(s) 18, 385, 579
BsmFI GGGAC 1 cut(s) 1257
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 1 cut(s) 664
Bsp119I TTCGAA 2 cut(s) 820, 945
Bsp1286I GDGCHC 2 cut(s) 237, 1156
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
Bsp19I CCATGG 1 cut(s) 212
BspACI CCGC 4 cut(s) 160, 166, 1167, 1261
BspANI GGCC 1 cut(s) 664
BspCNI CTCAG 5 cut(s) 73, 844, 865, 1035, 1260
BspLI GGNNCC 3 cut(s) 210, 297, 898
BspPI GGATC 3 cut(s) 290, 303, 385
BspT104I TTCGAA 2 cut(s) 820, 945
BsrBI CCGCTC 1 cut(s) 1261
BsrDI GCAATG 2 cut(s) 506, 1134
BsrFI RCCGGY 1 cut(s) 572
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 3 cut(s) 148, 926, 1260
BssAI RCCGGY 1 cut(s) 572
BssECI CCNNGG 6 cut(s) 203, 212, 290, 299, 528, 808
BssMI GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
BssSI CACGAG 2 cut(s) 14, 1053
BssT1I CCWWGG 2 cut(s) 212, 808
Bst2BI CACGAG 2 cut(s) 14, 1053
Bst2UI CCWGG 4 cut(s) 291, 395, 530, 909
Bst4CI ACNGT 2 cut(s) 29, 1097
Bst6I CTCTTC 2 cut(s) 320, 1284
BstAUI TGTACA 1 cut(s) 681
BstBI TTCGAA 2 cut(s) 820, 945
BstC8I GCNNGC 5 cut(s) 373, 574, 748, 1156, 1160
BstDEI CTNAG 8 cut(s) 81, 587, 643, 846, 852, 873, 1022, 1268
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 602, 778, 1237
BstHHI GCGC 1 cut(s) 752
BstKTI GATC 6 cut(s) 298, 393, 640, 1042, 1075, 1286
BstMBI GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
BstMWI GCNNNNNNNGC 2 cut(s) 208, 1151
BstNI CCWGG 4 cut(s) 291, 395, 530, 909
BstSCI CCNGG 4 cut(s) 289, 393, 528, 907
BstSFI CTRYAG 1 cut(s) 996
BstV1I GCAGC 5 cut(s) 174, 731, 947, 1034, 1093
BstV2I GAAGAC 6 cut(s) 135, 224, 357, 700, 885, 1205
BstX2I RGATCY 2 cut(s) 295, 390
BstYI RGATCY 2 cut(s) 295, 390
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 1 cut(s) 664
BtgI CCRYGG 1 cut(s) 212
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 602, 778, 1237
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 5 cut(s) 373, 574, 748, 1156, 1160
CaiI CAGNNNCTG 1 cut(s) 938
CfoI GCGC 1 cut(s) 752
Cfr10I RCCGGY 1 cut(s) 572
Csp6I GTAC 3 cut(s) 612, 682, 929
CviAII CATG 8 cut(s) 40, 49, 213, 308, 334, 356, 758, 1070
CviQI GTAC 3 cut(s) 612, 682, 929
DdeI CTNAG 8 cut(s) 81, 587, 643, 846, 852, 873, 1022, 1268
DpnI GATC 6 cut(s) 297, 392, 639, 1041, 1074, 1285
DpnII GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
DraI TTTAAA 1 cut(s) 1206
Eam1104I CTCTTC 2 cut(s) 320, 1284
EarI CTCTTC 2 cut(s) 320, 1284
EciI GGCGGA 1 cut(s) 1156
Ecl136II GAGCTC 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 212, 808
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 1156
Eco32I GATATC 1 cut(s) 923
Eco53kI GAGCTC 1 cut(s) 1154
Eco57I CTGAAG 2 cut(s) 729, 1264
EcoICRI GAGCTC 1 cut(s) 1154
EcoRII CCWGG 4 cut(s) 289, 393, 528, 907
EcoRV GATATC 1 cut(s) 923
EcoT14I CCWWGG 2 cut(s) 212, 808
EcoT38I GRGCYC 1 cut(s) 1156
ErhI CCWWGG 2 cut(s) 212, 808
FaeI CATG 8 cut(s) 43, 52, 216, 311, 337, 359, 761, 1073
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 1 cut(s) 1257
FatI CATG 8 cut(s) 39, 48, 212, 307, 333, 355, 757, 1069
FauNDI CATATG 1 cut(s) 270
FbaI TGATCA 1 cut(s) 1072
Fnu4HI GCNGC 7 cut(s) 163, 166, 720, 936, 1048, 1107, 1262
FokI GGATG 3 cut(s) 609, 765, 1244
FriOI GRGCYC 1 cut(s) 1156
Fsp4HI GCNGC 7 cut(s) 163, 166, 720, 936, 1048, 1107, 1262
FspBI CTAG 1 cut(s) 704
GlaI GCGC 1 cut(s) 751
GluI GCNGC 7 cut(s) 163, 166, 720, 936, 1048, 1107, 1262
GsuI CTGGAG 1 cut(s) 1277
HaeIII GGCC 1 cut(s) 664
HapII CCGG 1 cut(s) 573
HhaI GCGC 1 cut(s) 752
Hin1II CATG 8 cut(s) 43, 52, 216, 311, 337, 359, 761, 1073
Hin6I GCGC 1 cut(s) 750
HinP1I GCGC 1 cut(s) 750
HinfI GANTC 3 cut(s) 1001, 1239, 1270
HpaII CCGG 1 cut(s) 573
HphI GGTGA 3 cut(s) 37, 647, 964
Hpy166II GTNNAC 2 cut(s) 320, 682
Hpy188I TCNGA 8 cut(s) 76, 264, 303, 471, 834, 1269, 1283, 1288
Hpy188III TCNNGA 3 cut(s) 733, 1024, 1053
Hpy8I GTNNAC 2 cut(s) 320, 682
HpyAV CCTTC 1 cut(s) 1288
HpyCH4III ACNGT 2 cut(s) 29, 1097
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 7 cut(s) 499, 511, 617, 719, 1069, 1250, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 1151
HpyF3I CTNAG 8 cut(s) 81, 587, 643, 846, 852, 873, 1022, 1268
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 8 cut(s) 43, 52, 216, 311, 337, 359, 761, 1073
HspAI GCGC 1 cut(s) 750
KroI GCCGGC 1 cut(s) 572
KroNI GCCGGC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 1072
Kzo9I GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
LmnI GCTCC 4 cut(s) 208, 407, 902, 1258
Lsp1109I GCAGC 5 cut(s) 174, 731, 947, 1034, 1093
LweI GCATC 1 cut(s) 706
MaeI CTAG 1 cut(s) 704
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 2 cut(s) 624, 991
MalI GATC 6 cut(s) 297, 392, 639, 1041, 1074, 1285
MbiI CCGCTC 1 cut(s) 1261
MboI GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
MflI RGATCY 2 cut(s) 295, 390
MhlI GDGCHC 2 cut(s) 237, 1156
MlyI GAGTC 1 cut(s) 1279
MmeI TCCRAC 2 cut(s) 287, 1216
MroNI GCCGGC 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 485
MslI CAYNNNNRTG 2 cut(s) 447, 980
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 4 cut(s) 291, 395, 530, 909
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 4 cut(s) 291, 395, 530, 909
MwoI GCNNNNNNNGC 2 cut(s) 208, 1151
NaeI GCCGGC 1 cut(s) 574
NcoI CCATGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 270
NdeII GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
NgoMIV GCCGGC 1 cut(s) 572
NlaIII CATG 8 cut(s) 43, 52, 216, 311, 337, 359, 761, 1073
NlaIV GGNNCC 3 cut(s) 210, 297, 898
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 991
NspV TTCGAA 2 cut(s) 820, 945
PceI AGGCCT 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PctI GAATGC 1 cut(s) 748
PdiI GCCGGC 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 2 cut(s) 1001, 1239
PfoI TCCNGGA 1 cut(s) 907
PkrI GCNGC 7 cut(s) 164, 167, 721, 937, 1049, 1108, 1263
PleI GAGTC 1 cut(s) 1278
PpsI GAGTC 1 cut(s) 1278
PsiI TTATAA 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 1156
Psp6I CCWGG 4 cut(s) 289, 393, 528, 907
PspGI CCWGG 4 cut(s) 289, 393, 528, 907
PspN4I GGNNCC 3 cut(s) 210, 297, 898
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 2 cut(s) 295, 390
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 3 cut(s) 613, 683, 930
RsaNI GTAC 3 cut(s) 612, 682, 929
RseI CAYNNNNRTG 2 cut(s) 447, 980
SacI GAGCTC 1 cut(s) 1156
SatI GCNGC 7 cut(s) 163, 166, 720, 936, 1048, 1107, 1262
Sau3AI GATC 6 cut(s) 295, 390, 637, 1039, 1072, 1283
SchI GAGTC 1 cut(s) 1279
ScrFI CCNGG 4 cut(s) 291, 395, 530, 909
SduI GDGCHC 2 cut(s) 237, 1156
SfaNI GCATC 1 cut(s) 706
SfcI CTRYAG 1 cut(s) 996
SfuI TTCGAA 2 cut(s) 820, 945
SmiMI CAYNNNNRTG 2 cut(s) 447, 980
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 4 cut(s) 160, 166, 1167, 1261
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 1156
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 4 cut(s) 289, 393, 528, 907
StyI CCWWGG 2 cut(s) 212, 808
TaaI ACNGT 2 cut(s) 29, 1097
TaiI ACGT 1 cut(s) 655
TaqI TCGA 4 cut(s) 244, 537, 820, 945
TatI WGTACW 2 cut(s) 611, 681
TauI GCSGC 2 cut(s) 168, 1264
TfiI GAWTC 2 cut(s) 1001, 1239
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 1 cut(s) 991
TseI GCWGC 5 cut(s) 162, 719, 935, 1047, 1106
Tsp45I GTSAC 1 cut(s) 991
TspDTI ATGAA 6 cut(s) 28, 65, 117, 372, 774, 1050
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 1 cut(s) 480
XapI RAATTY 4 cut(s) 890, 902, 967, 1332
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.