Rh6AG096700

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000561
Physical Location & Seq
Reverse (-)
14244989 .. 14247734
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG096700.1

Sequence Viewer

Length: 1155 bp
ATGTTCGAGAATATTGACAGTCCTGAAGATTCTTCAGTCAAAAGCCAAGTTGAAGAGATCAAAGCAAAAATAGGAGTGGAATCCCCCATCTTTGTGAAGGCTATGCAGCCATCAAATGTTGCCAGTGGCTTTTTGTTGGCATTGCCTAAGCAATTCTGCCATTTGCATTTGCCATCCCATGATGCAACAATAATTTTACAAGATGAAAGCGGGGAGAAATACAGAACAAAGTTTATTGTGCAAAAGGACAAGAGCATACTGAGTAGTGGATGGAGAAGGTTTTGCTTTGCTAACAAGTTAGTTGTTGGTGATGTTTTAGTATTCCACTTGGTCAGGCCTTTAAAGTTCAAGGTGTACATAATGGGAATACATAGTTTAGCTGAAGTGGAAGCGGCTTTCAGCCTCTTGAAGTTCGGTTCCTGCACCAAACAAACAGCTTTTGAGGAAATACGTCCAATGCCTCTTCCAAAAGATGTCTGTCAAGTTCATATTGGTGAGAAGACAAGTTTGACATTAGATTCTAATGCTGCACTGATAGATGAGGAGTACAATGAAGAATTTGGCTTCAACATTTTAGAAGATGTTGGACTTCCAATACCAGCTACTGATTTTGGTGATGTTGAAAATACCGAGGATTTTACCATTATCGTGAATGGTGTGAGTATAAATTCTTGTCTCTCCGAAGAGATCAGAAATAGGTATTATGATCTATGCTGCTCACAAAGGTCATTTCTGCACAACCATCTCCTCAAAAGTATTAATGGTAAGTTAGCTGCTGAAATAATAACCGAAACAATTAACATTGCTAATGCCATTAGAGCTTGCAGGCTTTCCACCCCCCATGGTGCTTATGTATTGTGGGAGAAGACTTTAGATGCCTTTGAGATTTTGGGTATGAATGTTGGATTCTTACGTGCCCGGCTGAAGCGGCTTCTGAGCCTTTCTTTCAGGACCAAACAGGCTCTGATAAGGAAATTAAAGGAGGCTAAACTTGAGCAAGCACGTGCAAAGGACAAGGTGATAACTCTTGAATCCAAGTATTGGAAATGGAAAGAGAGGATGGTAAGTGCTGATGCTAAGATAGAGGCTCTAAAGGAAGTCGCAGAGAGGCGTGAACTTTTCTTCCAGGAAGAGGCTGTTGCTCCATGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

384

Amino Acids

43.5

Weight (kDa)

6.54

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 31 - 120 9.4e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1041
AciI CCGC 3 cut(s) 210, 392, 928
AcsI RAATTY 2 cut(s) 557, 667
AcuI CTGAAG 4 cut(s) 18, 45, 402, 944
AcvI CACGTG 1 cut(s) 1004
AfaI GTAC 2 cut(s) 356, 548
AfiI CCNNNNNNNGG 2 cut(s) 1041, 1132
AgsI TTSAA 6 cut(s) 53, 349, 409, 568, 623, 1031
AjnI CCWGG 1 cut(s) 1125
AloI GAACNNNNNNTCC 2 cut(s) 1107, 1139
AluBI AGCT 5 cut(s) 380, 437, 602, 773, 821
AluI AGCT 5 cut(s) 380, 437, 602, 773, 821
Alw26I GTCTC 1 cut(s) 680
AlwNI CAGNNNCTG 2 cut(s) 605, 964
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 106, 527, 714, 773
ApoI RAATTY 2 cut(s) 557, 667
AseI ATTAAT 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 951
AsuC2I CCSGG 1 cut(s) 919
AsuHPI GGTGA 4 cut(s) 320, 506, 626, 1030
AvaII GGWCC 1 cut(s) 951
BaeGI GKGCMC 1 cut(s) 919
BbrPI CACGTG 1 cut(s) 1004
BbsI GAAGAC 2 cut(s) 506, 872
BbvI GCAGC 4 cut(s) 118, 514, 701, 760
BccI CCATC 6 cut(s) 95, 118, 181, 264, 750, 1054
BciT130I CCWGG 1 cut(s) 1127
BcnI CCSGG 1 cut(s) 919
BcoDI GTCTC 1 cut(s) 680
BisI GCNGC 6 cut(s) 107, 393, 528, 715, 774, 929
BlsI GCNGC 6 cut(s) 108, 394, 529, 716, 775, 930
Bme1390I CCNGG 2 cut(s) 919, 1127
Bme18I GGWCC 1 cut(s) 951
BmgT120I GGNCC 1 cut(s) 951
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 2 cut(s) 919, 1127
BmsI GCATC 3 cut(s) 172, 865, 1063
BpiI GAAGAC 2 cut(s) 506, 872
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1013
BpuMI CCSGG 1 cut(s) 919
BsaAI YACGTR 2 cut(s) 914, 1004
BsaJI CCNNGG 3 cut(s) 630, 841, 1145
Bsc4I CCNNNNNNNGG 2 cut(s) 1041, 1132
Bse1I ACTGG 1 cut(s) 123
Bse3DI GCAATG 2 cut(s) 140, 801
BseBI CCWGG 1 cut(s) 1127
BseDI CCNNGG 3 cut(s) 630, 841, 1145
BseGI GGATG 3 cut(s) 173, 275, 1065
BseLI CCNNNNNNNGG 2 cut(s) 1041, 1132
BseMI GCAATG 2 cut(s) 140, 801
BseMII CTCAG 2 cut(s) 251, 926
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 2 cut(s) 557, 737
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 4 cut(s) 118, 514, 701, 760
BsgI GTGCAG 3 cut(s) 406, 513, 719
BshFI GGCC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 919
BslI CCNNNNNNNGG 2 cut(s) 1041, 1132
BsmAI GTCTC 1 cut(s) 680
BsnI GGCC 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 919
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 3 cut(s) 57, 687, 706
Bsp19I CCATGG 2 cut(s) 841, 1145
BspACI CCGC 3 cut(s) 210, 392, 928
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 252, 927
BspLI GGNNCC 1 cut(s) 418
BsrDI GCAATG 2 cut(s) 140, 801
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 3 cut(s) 630, 841, 1145
BssMI GATC 3 cut(s) 57, 687, 706
BssT1I CCWWGG 2 cut(s) 841, 1145
Bst2UI CCWGG 1 cut(s) 1127
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 4 cut(s) 48, 468, 678, 1125
BstAUI TGTACA 1 cut(s) 354
BstBAI YACGTR 2 cut(s) 914, 1004
BstC8I GCNNGC 3 cut(s) 823, 827, 999
BstDEI CTNAG 4 cut(s) 147, 260, 935, 1077
BstDSI CCRYGG 2 cut(s) 841, 1145
BstF5I GGATG 3 cut(s) 173, 275, 1065
BstKTI GATC 3 cut(s) 60, 690, 709
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 3 cut(s) 57, 687, 706
BstMWI GCNNNNNNNGC 2 cut(s) 818, 928
BstNI CCWGG 1 cut(s) 1127
BstSCI CCNGG 2 cut(s) 917, 1125
BstSLI GKGCMC 1 cut(s) 919
BstV1I GCAGC 4 cut(s) 118, 514, 701, 760
BstV2I GAAGAC 2 cut(s) 506, 872
BsuRI GGCC 1 cut(s) 337
BtgI CCRYGG 2 cut(s) 841, 1145
BtsCI GGATG 3 cut(s) 173, 275, 1065
BtsIMutI CAGTG 2 cut(s) 130, 530
Cac8I GCNNGC 3 cut(s) 823, 827, 999
CaiI CAGNNNCTG 2 cut(s) 605, 964
Cfr13I GGNCC 1 cut(s) 951
Csp6I GTAC 2 cut(s) 355, 547
CviAII CATG 3 cut(s) 179, 842, 1146
CviQI GTAC 2 cut(s) 355, 547
DdeI CTNAG 4 cut(s) 147, 260, 935, 1077
DpnI GATC 3 cut(s) 59, 689, 708
DpnII GATC 3 cut(s) 57, 687, 706
DraI TTTAAA 1 cut(s) 342
Eam1104I CTCTTC 4 cut(s) 48, 468, 678, 1125
EarI CTCTTC 4 cut(s) 48, 468, 678, 1125
Eco130I CCWWGG 2 cut(s) 841, 1145
Eco147I AGGCCT 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 951
Eco57I CTGAAG 4 cut(s) 18, 45, 402, 944
Eco72I CACGTG 1 cut(s) 1004
EcoRII CCWGG 1 cut(s) 1125
EcoT14I CCWWGG 2 cut(s) 841, 1145
ErhI CCWWGG 2 cut(s) 841, 1145
FaeI CATG 3 cut(s) 182, 845, 1149
FatI CATG 3 cut(s) 178, 841, 1145
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 6 cut(s) 107, 393, 528, 715, 774, 929
FokI GGATG 3 cut(s) 160, 282, 1072
Fsp4HI GCNGC 6 cut(s) 107, 393, 528, 715, 774, 929
GluI GCNGC 6 cut(s) 107, 393, 528, 715, 774, 929
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 919
Hin1II CATG 3 cut(s) 182, 845, 1149
HinfI GANTC 5 cut(s) 29, 80, 518, 906, 1031
HpaII CCGG 1 cut(s) 919
HphI GGTGA 4 cut(s) 320, 506, 626, 1030
Hpy166II GTNNAC 2 cut(s) 355, 1115
Hpy188I TCNGA 4 cut(s) 682, 692, 936, 966
Hpy188III TCNNGA 6 cut(s) 7, 23, 406, 649, 949, 1028
Hpy8I GTNNAC 2 cut(s) 355, 1115
HpyAV CCTTC 2 cut(s) 91, 270
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 3 cut(s) 451, 913, 1003
HpyCH4V TGCA 9 cut(s) 106, 166, 185, 241, 423, 530, 736, 825, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 818, 928
HpyF3I CTNAG 4 cut(s) 147, 260, 935, 1077
HpySE526I ACGT 3 cut(s) 451, 913, 1003
Hsp92II CATG 3 cut(s) 182, 845, 1149
Kzo9I GATC 3 cut(s) 57, 687, 706
LmnI GCTCC 1 cut(s) 1147
Lsp1109I GCAGC 4 cut(s) 118, 514, 701, 760
LweI GCATC 3 cut(s) 172, 865, 1063
MaeII ACGT 3 cut(s) 451, 913, 1003
MalI GATC 3 cut(s) 59, 689, 708
MboI GATC 3 cut(s) 57, 687, 706
MhlI GDGCHC 1 cut(s) 919
MluCI AATT 6 cut(s) 152, 192, 557, 667, 795, 974
MmeI TCCRAC 2 cut(s) 565, 883
MseI TTAA 4 cut(s) 341, 759, 798, 977
MslI CAYNNNNRTG 4 cut(s) 92, 492, 647, 1150
MspI CCGG 1 cut(s) 919
MspR9I CCNGG 2 cut(s) 919, 1127
MvaI CCWGG 1 cut(s) 1127
MwoI GCNNNNNNNGC 2 cut(s) 818, 928
NciI CCSGG 1 cut(s) 919
NcoI CCATGG 2 cut(s) 841, 1145
NdeII GATC 3 cut(s) 57, 687, 706
NlaIII CATG 3 cut(s) 182, 845, 1149
NlaIV GGNNCC 1 cut(s) 418
PceI AGGCCT 1 cut(s) 337
PfeI GAWTC 5 cut(s) 29, 80, 518, 906, 1031
PflMI CCANNNNNTGG 1 cut(s) 1041
PfoI TCCNGGA 1 cut(s) 1125
PkrI GCNGC 6 cut(s) 108, 394, 529, 716, 775, 930
PmaCI CACGTG 1 cut(s) 1004
PmlI CACGTG 1 cut(s) 1004
Ppu21I YACGTR 2 cut(s) 914, 1004
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 1125
PspCI CACGTG 1 cut(s) 1004
PspGI CCWGG 1 cut(s) 1125
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 951
PstNI CAGNNNCTG 2 cut(s) 605, 964
RsaI GTAC 2 cut(s) 356, 548
RsaNI GTAC 2 cut(s) 355, 547
RseI CAYNNNNRTG 4 cut(s) 92, 492, 647, 1150
SaqAI TTAA 4 cut(s) 341, 759, 798, 977
SatI GCNGC 6 cut(s) 107, 393, 528, 715, 774, 929
Sau3AI GATC 3 cut(s) 57, 687, 706
Sau96I GGNCC 1 cut(s) 951
ScrFI CCNGG 2 cut(s) 919, 1127
SduI GDGCHC 1 cut(s) 919
SfaNI GCATC 3 cut(s) 172, 865, 1063
SinI GGWCC 1 cut(s) 951
SmiMI CAYNNNNRTG 4 cut(s) 92, 492, 647, 1150
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
Sse9I AATT 6 cut(s) 152, 192, 557, 667, 795, 974
SseBI AGGCCT 1 cut(s) 337
SsiI CCGC 3 cut(s) 210, 392, 928
SspI AATATT 1 cut(s) 13
StuI AGGCCT 1 cut(s) 337
StyD4I CCNGG 2 cut(s) 917, 1125
StyI CCWWGG 2 cut(s) 841, 1145
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 3 cut(s) 454, 916, 1006
TaqI TCGA 1 cut(s) 6
TasI AATT 6 cut(s) 152, 192, 557, 667, 795, 974
TatI WGTACW 2 cut(s) 354, 546
TauI GCSGC 2 cut(s) 395, 931
TfiI GAWTC 5 cut(s) 29, 80, 518, 906, 1031
Tru1I TTAA 4 cut(s) 341, 759, 798, 977
Tru9I TTAA 4 cut(s) 341, 759, 798, 977
TscAI CASTG 2 cut(s) 130, 537
TseI GCWGC 4 cut(s) 106, 527, 714, 773
TspDTI ATGAA 4 cut(s) 219, 476, 567, 911
TspRI CASTG 2 cut(s) 130, 537
Van91I CCANNNNNTGG 1 cut(s) 1041
VpaK11BI GGWCC 1 cut(s) 951
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 2 cut(s) 557, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.