Rroxscaffold_7G00205340

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000561
Physical Location & Seq
Reverse (-)
54899353 .. 54901930
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205340.1

Sequence Viewer

Length: 993 bp
ATGCTCCAACACAAAATGAATGACAGGGAGGAGGAAATGTCCGAGGATTTCCGAGAGGAAAACGGGGAGGAGGAGAAGGACAGGATGGACATTTCCCCGCTCTCTGTCACCAGACTACAATTTGACAATTTGACCCTCCATTCAACATTCTCGGTAGAGCAGAGGAAGCGCTCTAAGATCCGGACAAGTCGATTTAGTCCAACCCAAATTGTCAAATTCCACAATTTGACCCTTGATTCTCCAAACTCGGAACAGAGGCAGCCATTGGCGGAAGCTAACAAATATGGGTTCAAAACTCCCAAAGGGAAAAGACATTTGAGTCCAGGCCTTAACACCAAGCGAGAAGTTTCTAGAGATCCTCAGAGGCATGGAGGCTGCAAACGGAGTCGAACCAACCCGTCCATGTCCTGGCATTTTGGCCATCCTGAAGAGTCTTCTGTCAAAAGCCAAGTTAGAGAAATCCCAGAGAATCTAGAAGCCGAATGTCCCTTCTTTATGAAGTCCATGATACGATCAAATATTTCCGTTTGCTTTATGTTGTCACTGCCTAGGAGATTCTGCAACTTGCATTTACCTTTGCATGGTACAGCAACGATAACTTTGGAAGATAAAGAGGGGAAAGAATATGAAACAAAATTTCTTGCGGACAAAAGGACGCTGAGTGGTGGATGGAAAGGATTTTGTCAGGCAAAAGACTTGCGTCTTGATGATGTTCTAGTTTTCCAGTTGGTTAGCCCTTTGAAGTTCAAGCTTATTCTTCTGACTTTGATATATTGCTGTGTCTACACACTGTTGCAGGTTGGAAAACCCTCCAATGCAGAGGAAAACCCTGCAAACCTGACAAGCTATTCATTTGTAGATGATGATGAGTCTGAGGATCAGATTACAGAGATAAGGAATTTGGCTCCAATCACGGTGGTGCATTTAATCCGGCCAGGACAAGCTGCTGATTTTGAAGATGTTAGAAGTTCTGAGAATTTCGCCAGTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

330

Amino Acids

37.92

Weight (kDa)

6.57

Isoelectric Point (pI)

60.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 165 - 252 9.3e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 408
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 783
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 3 cut(s) 98, 269, 644
AclWI GGATC 3 cut(s) 172, 350, 885
AcoI YGGCCR 2 cut(s) 418, 932
AcsI RAATTY 4 cut(s) 215, 635, 898, 976
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 2 cut(s) 586, 988
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 408, 581
AgsI TTSAA 5 cut(s) 144, 292, 742, 748, 956
AjnI CCWGG 3 cut(s) 322, 407, 934
AleI CACNNNNGTG 1 cut(s) 917
AluBI AGCT 4 cut(s) 275, 751, 846, 944
AluI AGCT 4 cut(s) 275, 751, 846, 944
AlwI GGATC 3 cut(s) 172, 350, 885
Aor13HI TCCGGA 1 cut(s) 180
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 3 cut(s) 325, 418, 932
ApeKI GCWGC 3 cut(s) 259, 375, 944
ApoI RAATTY 4 cut(s) 215, 635, 898, 976
AspA2I CCTAGG 1 cut(s) 548
AspLEI GCGC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 100
AvrII CCTAGG 1 cut(s) 548
BalI TGGCCA 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 271, 362, 931
BccI CCATC 3 cut(s) 79, 429, 663
BciT130I CCWGG 3 cut(s) 324, 409, 936
BfaI CTAG 4 cut(s) 351, 473, 549, 716
BfoI RGCGCY 1 cut(s) 172
BfuAI ACCTGC 1 cut(s) 787
BisI GCNGC 3 cut(s) 260, 376, 945
BlnI CCTAGG 1 cut(s) 548
BlsI GCNGC 3 cut(s) 261, 377, 946
Bme1390I CCNGG 3 cut(s) 324, 409, 936
BmiI GGNNCC 1 cut(s) 906
BmrFI CCNGG 3 cut(s) 324, 409, 936
BoxI GACNNNNGTC 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 990
BsaJI CCNNGG 2 cut(s) 42, 548
BsaWI WCCGGW 1 cut(s) 180
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 408, 581
Bse1I ACTGG 2 cut(s) 724, 984
BseAI TCCGGA 1 cut(s) 180
BseBI CCWGG 3 cut(s) 324, 409, 936
BseDI CCNNGG 2 cut(s) 42, 548
BseGI GGATG 3 cut(s) 90, 421, 674
BseLI CCNNNNNNNGG 2 cut(s) 408, 581
BseMII CTCAG 4 cut(s) 374, 650, 864, 963
BseNI ACTGG 2 cut(s) 724, 984
BseRI GAGGAG 3 cut(s) 44, 83, 86
BseXI GCAGC 3 cut(s) 271, 362, 931
BshFI GGCC 3 cut(s) 327, 420, 934
BsiSI CCGG 2 cut(s) 181, 931
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 2 cut(s) 408, 581
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 3 cut(s) 327, 420, 934
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 4 cut(s) 177, 355, 512, 877
BspACI CCGC 3 cut(s) 98, 269, 644
BspANI GGCC 3 cut(s) 327, 420, 934
BspCNI CTCAG 4 cut(s) 373, 651, 865, 964
BspEI TCCGGA 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 906
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 3 cut(s) 172, 350, 885
BsrBI CCGCTC 1 cut(s) 100
BsrI ACTGG 2 cut(s) 724, 984
BssECI CCNNGG 2 cut(s) 42, 548
BssMI GATC 4 cut(s) 177, 355, 512, 877
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 3 cut(s) 324, 409, 936
Bst4CI ACNGT 2 cut(s) 792, 916
Bst6I CTCTTC 1 cut(s) 423
BstBAI YACGTR 1 cut(s) 990
BstDEI CTNAG 5 cut(s) 174, 360, 659, 873, 972
BstF5I GGATG 3 cut(s) 90, 421, 674
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 4 cut(s) 180, 358, 515, 880
BstMBI GATC 4 cut(s) 177, 355, 512, 877
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 3 cut(s) 324, 409, 936
BstPAI GACNNNNGTC 1 cut(s) 699
BstSCI CCNGG 3 cut(s) 322, 407, 934
BstV1I GCAGC 3 cut(s) 271, 362, 931
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 2 cut(s) 177, 355
BstYI RGATCY 2 cut(s) 177, 355
BsuRI GGCC 3 cut(s) 327, 420, 934
BtsCI GGATG 3 cut(s) 90, 421, 674
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 2 cut(s) 542, 788
BveI ACCTGC 1 cut(s) 787
CfoI GCGC 1 cut(s) 171
CseI GACGC 2 cut(s) 664, 689
Csp6I GTAC 2 cut(s) 585, 987
CspCI CAANNNNNGTGG 2 cut(s) 897, 932
CviAII CATG 4 cut(s) 368, 403, 505, 581
CviQI GTAC 2 cut(s) 585, 987
DdeI CTNAG 5 cut(s) 174, 360, 659, 873, 972
DpnI GATC 4 cut(s) 179, 357, 514, 879
DpnII GATC 4 cut(s) 177, 355, 512, 877
DrdI GACNNNNNNGTC 1 cut(s) 318
DseDI GACNNNNNNGTC 1 cut(s) 318
EaeI YGGCCR 2 cut(s) 418, 932
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 548
Eco147I AGGCCT 1 cut(s) 327
Eco47III AGCGCT 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 447
EcoRII CCWGG 3 cut(s) 322, 407, 934
EcoT14I CCWWGG 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 4 cut(s) 371, 406, 508, 584
FaiI YATR 9 cut(s) 285, 369, 404, 497, 506, 536, 582, 627, 772
FaqI GGGAC 1 cut(s) 471
FatI CATG 4 cut(s) 367, 402, 504, 580
FauI CCCGC 1 cut(s) 105
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 3 cut(s) 260, 376, 945
FokI GGATG 3 cut(s) 97, 408, 681
Fsp4HI GCNGC 3 cut(s) 260, 376, 945
FspBI CTAG 4 cut(s) 351, 473, 549, 716
GlaI GCGC 1 cut(s) 170
GluI GCNGC 3 cut(s) 260, 376, 945
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 327, 420, 934
HapII CCGG 2 cut(s) 181, 931
HgaI GACGC 2 cut(s) 664, 689
HhaI GCGC 1 cut(s) 171
Hin1II CATG 4 cut(s) 371, 406, 508, 584
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 7 cut(s) 236, 319, 385, 431, 469, 555, 869
HpaII CCGG 2 cut(s) 181, 931
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 8 cut(s) 43, 53, 250, 363, 762, 874, 882, 973
Hpy188III TCNNGA 5 cut(s) 181, 351, 425, 473, 704
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 2 cut(s) 70, 499
HpyCH4III ACNGT 2 cut(s) 792, 916
HpyCH4IV ACGT 1 cut(s) 989
HpyCH4V TGCA 8 cut(s) 378, 561, 568, 580, 796, 818, 833, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 5 cut(s) 174, 360, 659, 873, 972
HpySE526I ACGT 1 cut(s) 989
Hsp92II CATG 4 cut(s) 371, 406, 508, 584
HspAI GCGC 1 cut(s) 169
Kpn2I TCCGGA 1 cut(s) 180
Kzo9I GATC 4 cut(s) 177, 355, 512, 877
LmnI GCTCC 2 cut(s) 9, 910
Lsp1109I GCAGC 3 cut(s) 271, 362, 931
MaeI CTAG 4 cut(s) 351, 473, 549, 716
MaeII ACGT 1 cut(s) 989
MaeIII GTNAC 2 cut(s) 106, 540
MalI GATC 4 cut(s) 179, 357, 514, 879
MbiI CCGCTC 1 cut(s) 100
MboI GATC 4 cut(s) 177, 355, 512, 877
MboII GAAGA 5 cut(s) 426, 440, 617, 749, 968
MflI RGATCY 2 cut(s) 177, 355
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 8 cut(s) 119, 127, 207, 215, 223, 635, 898, 976
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 4 cut(s) 328, 394, 440, 878
MmeI TCCRAC 3 cut(s) 31, 224, 781
Mox20I TGGCCA 1 cut(s) 420
MroI TCCGGA 1 cut(s) 180
MscI TGGCCA 1 cut(s) 420
MseI TTAA 2 cut(s) 330, 926
MslI CAYNNNNRTG 1 cut(s) 917
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 2 cut(s) 181, 931
MspR9I CCNGG 3 cut(s) 324, 409, 936
MvaI CCWGG 3 cut(s) 324, 409, 936
MwoI GCNNNNNNNGC 1 cut(s) 166
NdeII GATC 4 cut(s) 177, 355, 512, 877
NlaIII CATG 4 cut(s) 371, 406, 508, 584
NlaIV GGNNCC 1 cut(s) 906
NmuCI GTSAC 2 cut(s) 106, 540
OliI CACNNNNGTG 1 cut(s) 917
PceI AGGCCT 1 cut(s) 327
PfeI GAWTC 3 cut(s) 236, 469, 555
PflMI CCANNNNNTGG 1 cut(s) 408
PkrI GCNGC 3 cut(s) 261, 377, 946
PleI GAGTC 4 cut(s) 327, 393, 439, 877
PpsI GAGTC 4 cut(s) 327, 393, 439, 877
Ppu21I YACGTR 1 cut(s) 990
PshAI GACNNNNGTC 1 cut(s) 699
Psp6I CCWGG 3 cut(s) 322, 407, 934
PspGI CCWGG 3 cut(s) 322, 407, 934
PspN4I GGNNCC 1 cut(s) 906
PsuI RGATCY 2 cut(s) 177, 355
RsaI GTAC 2 cut(s) 586, 988
RsaNI GTAC 2 cut(s) 585, 987
RseI CAYNNNNRTG 1 cut(s) 917
SaqAI TTAA 2 cut(s) 330, 926
SatI GCNGC 3 cut(s) 260, 376, 945
Sau3AI GATC 4 cut(s) 177, 355, 512, 877
SchI GAGTC 4 cut(s) 328, 394, 440, 878
ScrFI CCNGG 3 cut(s) 324, 409, 936
SetI ASST 8 cut(s) 277, 577, 753, 801, 840, 848, 946, 992
SmiMI CAYNNNNRTG 1 cut(s) 917
Sse9I AATT 8 cut(s) 119, 127, 207, 215, 223, 635, 898, 976
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 3 cut(s) 98, 269, 644
SspI AATATT 1 cut(s) 520
SspMI CTAG 4 cut(s) 351, 473, 549, 716
StuI AGGCCT 1 cut(s) 327
StyD4I CCNGG 3 cut(s) 322, 407, 934
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 2 cut(s) 792, 916
TaiI ACGT 1 cut(s) 992
TaqI TCGA 2 cut(s) 190, 388
TasI AATT 8 cut(s) 119, 127, 207, 215, 223, 635, 898, 976
TfiI GAWTC 3 cut(s) 236, 469, 555
Tru1I TTAA 2 cut(s) 330, 926
Tru9I TTAA 2 cut(s) 330, 926
TscAI CASTG 2 cut(s) 549, 795
TseFI GTSAC 2 cut(s) 106, 540
TseI GCWGC 3 cut(s) 259, 375, 944
Tsp45I GTSAC 2 cut(s) 106, 540
TspDTI ATGAA 4 cut(s) 32, 512, 642, 840
TspGWI ACGGA 2 cut(s) 397, 514
TspRI CASTG 2 cut(s) 549, 795
Van91I CCANNNNNTGG 1 cut(s) 408
XapI RAATTY 4 cut(s) 215, 635, 898, 976
XbaI TCTAGA 2 cut(s) 350, 472
XmaJI CCTAGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 783
XspI CTAG 4 cut(s) 351, 473, 549, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.