Rorug05G0578200

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000561
Physical Location & Seq
Forward (+)
77416564 .. 77419391
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0578200.1

Sequence Viewer

Length: 1833 bp
ATGGCTACTGTGGATTCTCTCAACTGCACCAATGAGGAGGTGAGAGATCAAGACAATAAGACCAATGGAGAATCAGAAGCTGTGAGTAGAAACTTCCATGAAAATGAACTGAAGGGGACAACTGCTAAAACCAAGGCGTCCATTATCAGAAAGAGCACAGGAGGCTGGACTTTTGCAAGTCTCTTGCTAGTGAATCAGGGGCTAGCAACACTAGCTTTCTTTGGTGTTGGTGTGAACTTAGTTTTGTTCTTGACCAGAGTTCTTGATCAAGAGAATGCCGTTGCTGCAAACAGTGTGAGCAAGTGGACTGGAACTGTTTATTTGTGCTCCCTAATTGGTGCATTTCTCAGTGACTCTTATTGGGGCCGCTACTTAACATGTGCAGTCTTTCAGCTCATCTTTCTACTGGGATTGGTGCTATTATCACTGACGTCTTGGCTCTTCTTGTTCCATCCTTCTGGTTGTGGAGATGAGGAACTAGTATGCTCGCCCACATCATCAATTGGAGTTGCTATCTTTTACTCATCAATATATTTAGTTGCATTGGGATATGGTGGGTACCAACCTACTATAGCAACCTTCGGATCAGACCAATTTGATGAGGCAAACCCTAAAGAAATAGCTTCGAAAGCAGTTTTCTTTTCCTATTTCTACTTTGCACTCAATCTTGGGTCTCTTTTCTCGAACACCATATTGGTTTTTTACGAGGATACAGGCAGATGGACTATTGGGTTTGTGGCGTCATTGGGATCTGCTGTCTTGGCCCTATTGTTCTTTTTGTTGGGGACACCAGGGTATAGGTACTTAAAACCGTGTGGCAACCCTTTGCCACGTGTCGCTCAGGTATTTGTGGCTGCTGCTAGAAAATGGGATGTCGTTCCTTCCAATTCAGGGGACTTGTATGAAGTGGAAGGACCAGAATCTGCCATCAGAGGAAGCAGAAAGATTTTTCACAGTAATGAGATTGCGTTCTTGGATAAGGCAGCAACAATAACAAATCATGATCTACGTGGTCCAAATGATCCATGGAGGTTATGCACTGTAACTCAAGTTGAAGAAGCTAAATGTGTTATGAAAATGCTACCCATTTGGCTTTGTACCATAATTTACTCAGTCGTCTTCACACAAATGGCCTCCCTTTTCGTAGAACAAGGTAATGTGATGAAATCCGACATTGGAAGTTTTCACCTCCCAGCTGCTAGCATGTCTGCTTTTGACATATGCAGTGTTCTAATCTGCACTGGTATCTATCGCCAAGTTCTCGTGCCTTTAGCTGGAAAGCTAAGCGGAAACCCTAAAGGCTTAACCGAGCTTCAAAGAATGGGAATCGGGCTCCTTATCGGAATGTTATCAATGGTTGCAGCTGGTGCCACAGAGATTGCAAGGCTCAAACATGTGGTCCGTGGTGAGAAGATAAGCTCTCTAAGCATATTCTGGCAAATTCCACAATATGTTCTTGTTGGTGCTTCTGAAGTCTTTATGTATGTGGGTCAACTTGAATTCTTCAATGGTCAGGCCCCAGATGGGATCAAAAGCTTTGGAAGTTCACTATGCATGGCTTCAATTTCTCTTGGGAATTATGTGAGCAGCTTGCTAGTTATAATGGTGATGGGAATTACAGCTAGAGGTAAAAGCCCAGGATGGATCCCAGATGATCTAAATACAGGTCATATGGATAGGTTCTACTTCCTGCTTGCAGTACTAGCAGCTTTTGACTTTGTGATTTACGTGTATTGTGCAAAGTGGTATAAATGCATTAACCTCCAAGACTCTGATGAGAAGGAAATTCAAATCATGGAAAGCCAAGAAGATGTGGTGCTCAAAAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

610

Amino Acids

66.88

Weight (kDa)

5.65

Isoelectric Point (pI)

25.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 125 - 548 3.5e-85 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000561)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06320 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330 FvH4_2g06330
malus_domestica MD05G1077500.v1.1 MD10G1092700.v1.1
prunus_persica Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1 Prupe.8G122500_v2.0.a1
pyrus_communis pycom10g07620
rosa_chinensis RchiOBHm_Chr2g0147541 RchiOBHm_Chr2g0168211 RchiOBHm_Chr6g0256791 RchiOBHm_Chr6g0258071 RchiOBHm_Chr6g0258081 RchiOBHm_Chr6g0258121 RchiOBHm_Chr6g0258181 RchiOBHm_Chr6g0258201 RchiOBHm_Chr6g0258211
rosa_laevigata RLG00000014639 RLG00000014642 RLG00000014647
rosa_multiflora Rmu_sc0006058.1_g000005 Rmu_sc0007933.1_g000012
rosa_roxburghii Rroxscaffold_7G00205330 Rroxscaffold_7G00205340 Rroxscaffold_7G00208080 Rroxscaffold_7G00208100 Rroxscaffold_7G00208160
rosa_rugosa Rorug05G0578100 Rorug05G0578200 Rorug05G0578300 Rorug05G0578400 Rorug05G0578400 Rorug05G0578500 Rorug05G0578600 Rorug05G0578700 Rorug05G0578800 Rorug05G0578900 Rorug05G0579000 Rorug05G0579300 Rorug05G0579300 Rorug05G0579400
rosa_samantha Rh2BG613900 Rh4BG034900 Rh6AG096700 Rh6AG097000 Rh6AG097100 Rh6AG097300 Rh6BG087600 Rh6BG088200 Rh6BG088300 Rh6CG083700 Rh6CG083900 Rh6CG084000 Rh6CG084200 Rh6DG079500 Rh6DG080000 Rh6DG080200 Rh6DG080400 Rh6DG164000 Rh7BG391100 Rh7CG434700 Rh7CG467700
rosa_wichuraiana Rw0G021370 Rw0G021380 Rw6G008220 Rw6G008240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1601
AatII GACGTC 1 cut(s) 434
Acc65I GGTACC 1 cut(s) 558
AccB1I GGYRCC 2 cut(s) 558, 1367
AciI CCGC 2 cut(s) 367, 1287
AclWI GGATC 6 cut(s) 592, 757, 1016, 1535, 1639, 1652
AcsI RAATTY 3 cut(s) 1440, 1499, 1785
AcuI CTGAAG 2 cut(s) 131, 1491
AcvI CACGTG 1 cut(s) 833
AcyI GRCGYC 3 cut(s) 137, 431, 740
AfaI GTAC 4 cut(s) 560, 803, 1099, 1701
AfiI CCNNNNNNNGG 3 cut(s) 891, 1274, 1524
AflIII ACRYGT 4 cut(s) 377, 832, 1393, 1728
AgsI TTSAA 6 cut(s) 1055, 1316, 1499, 1507, 1563, 1790
AhlI ACTAGT 1 cut(s) 478
AjnI CCWGG 2 cut(s) 790, 1636
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
Alw21I GWGCWC 3 cut(s) 158, 329, 1821
Alw26I GTCTC 2 cut(s) 185, 678
AlwI GGATC 6 cut(s) 592, 757, 1016, 1535, 1639, 1652
AlwNI CAGNNNCTG 2 cut(s) 80, 923
AoxI GGCC 4 cut(s) 364, 762, 1131, 1515
ApeKI GCWGC 8 cut(s) 284, 854, 857, 983, 1196, 1361, 1587, 1706
ApoI RAATTY 3 cut(s) 1440, 1499, 1785
ArsI GACNNNNNNTTYG 2 cut(s) 1383, 1415
Asp718I GGTACC 1 cut(s) 558
AspS9I GGNCC 6 cut(s) 364, 763, 914, 1013, 1399, 1516
AsuHPI GGTGA 4 cut(s) 52, 1178, 1418, 1618
AsuII TTCGAA 1 cut(s) 626
AsuNHI GCTAGC 2 cut(s) 202, 1199
AvaII GGWCC 3 cut(s) 914, 1013, 1399
BamHI GGATCC 1 cut(s) 1644
BanI GGYRCC 2 cut(s) 558, 1367
BanII GRGCYC 1 cut(s) 1335
BauI CACGAG 1 cut(s) 1262
BbrPI CACGTG 1 cut(s) 833
BbsI GAAGAC 1 cut(s) 1111
Bbv12I GWGCWC 3 cut(s) 158, 329, 1821
BbvI GCAGC 8 cut(s) 271, 841, 844, 995, 1183, 1373, 1599, 1718
BccI CCATC 6 cut(s) 459, 714, 935, 1517, 1603, 1635
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 792, 1638
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 185, 678
BcuI ACTAGT 1 cut(s) 478
BfaI CTAG 9 cut(s) 188, 203, 212, 479, 861, 1200, 1595, 1623, 1703
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 9 cut(s) 285, 367, 855, 858, 984, 1197, 1362, 1588, 1707
BlpI GCTNAGC 1 cut(s) 1283
BlsI GCNGC 9 cut(s) 286, 368, 856, 859, 985, 1198, 1363, 1589, 1708
BmcAI AGTACT 1 cut(s) 1701
Bme1390I CCNGG 2 cut(s) 792, 1638
Bme18I GGWCC 3 cut(s) 914, 1013, 1399
BmgT120I GGNCC 6 cut(s) 364, 763, 914, 1013, 1399, 1516
BmiI GGNNCC 6 cut(s) 365, 560, 1334, 1369, 1518, 1646
BmrFI CCNGG 2 cut(s) 792, 1638
BmrI ACTGGG 1 cut(s) 416
BmtI GCTAGC 2 cut(s) 206, 1203
BmuI ACTGGG 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 1111
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 1283
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 1032
BsaAI YACGTR 3 cut(s) 833, 1010, 1729
BsaHI GRCGYC 3 cut(s) 137, 431, 740
BsaI GGTCTC 1 cut(s) 678
BsaJI CCNNGG 5 cut(s) 132, 791, 1025, 1402, 1636
Bsc4I CCNNNNNNNGG 3 cut(s) 891, 1274, 1524
Bse1I ACTGG 3 cut(s) 313, 411, 1246
BseBI CCWGG 2 cut(s) 792, 1638
BseDI CCNNGG 5 cut(s) 132, 791, 1025, 1402, 1636
BseGI GGATG 3 cut(s) 451, 877, 1646
BseLI CCNNNNNNNGG 3 cut(s) 891, 1274, 1524
BseMII CTCAG 3 cut(s) 361, 854, 1125
BseNI ACTGG 3 cut(s) 313, 411, 1246
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 8 cut(s) 271, 841, 844, 995, 1183, 1373, 1599, 1718
BseYI CCCAGC 1 cut(s) 1192
BsgI GTGCAG 3 cut(s) 10, 402, 1222
BshFI GGCC 4 cut(s) 366, 764, 1133, 1517
BshNI GGYRCC 2 cut(s) 558, 1367
BsiHKAI GWGCWC 3 cut(s) 158, 329, 1821
BslFI GGGAC 3 cut(s) 130, 799, 908
BslI CCNNNNNNNGG 3 cut(s) 891, 1274, 1524
BsmAI GTCTC 2 cut(s) 185, 678
BsmFI GGGAC 3 cut(s) 130, 799, 908
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 4 cut(s) 366, 764, 1133, 1517
Bso31I GGTCTC 1 cut(s) 678
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 4 cut(s) 158, 329, 1335, 1821
Bsp143I GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
Bsp1720I GCTNAGC 1 cut(s) 1283
Bsp19I CCATGG 1 cut(s) 1025
BspACI CCGC 2 cut(s) 367, 1287
BspANI GGCC 4 cut(s) 366, 764, 1133, 1517
BspCNI CTCAG 3 cut(s) 360, 853, 1124
BspHI TCATGA 1 cut(s) 1000
BspLI GGNNCC 6 cut(s) 365, 560, 1334, 1369, 1518, 1646
BspOI GCTAGC 2 cut(s) 206, 1203
BspPI GGATC 6 cut(s) 592, 757, 1016, 1535, 1639, 1652
BspQI GCTCTTC 1 cut(s) 446
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 2 cut(s) 558, 1367
BspTNI GGTCTC 1 cut(s) 678
BsrI ACTGG 3 cut(s) 313, 411, 1246
BssECI CCNNGG 5 cut(s) 132, 791, 1025, 1402, 1636
BssMI GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
BssNI GRCGYC 3 cut(s) 137, 431, 740
BssSI CACGAG 1 cut(s) 1262
BssT1I CCWWGG 2 cut(s) 132, 1025
Bst2BI CACGAG 1 cut(s) 1262
Bst2UI CCWGG 2 cut(s) 792, 1638
Bst4CI ACNGT 6 cut(s) 10, 293, 316, 813, 956, 1042
Bst6I CTCTTC 1 cut(s) 446
BstACI GRCGYC 3 cut(s) 137, 431, 740
BstAPI GCANNNNNTGC 1 cut(s) 1367
BstBAI YACGTR 3 cut(s) 833, 1010, 1729
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 5 cut(s) 204, 488, 1201, 1592, 1695
BstDEI CTNAG 6 cut(s) 238, 347, 840, 1111, 1283, 1424
BstDSI CCRYGG 2 cut(s) 1025, 1402
BstF5I GGATG 3 cut(s) 451, 877, 1646
BstKTI GATC 9 cut(s) 49, 268, 587, 752, 1006, 1024, 1530, 1647, 1657
BstMAI GTCTC 2 cut(s) 185, 678
BstMBI GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
BstMWI GCNNNNNNNGC 8 cut(s) 162, 212, 284, 629, 761, 1367, 1425, 1703
BstNI CCWGG 2 cut(s) 792, 1638
BstNSI RCATGY 3 cut(s) 381, 1207, 1397
BstSCI CCNGG 2 cut(s) 790, 1636
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 8 cut(s) 271, 841, 844, 995, 1183, 1373, 1599, 1718
BstV2I GAAGAC 1 cut(s) 1111
BstX2I RGATCY 2 cut(s) 749, 1644
BstXI CCANNNNNNTGG 1 cut(s) 458
BstYI RGATCY 2 cut(s) 749, 1644
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 4 cut(s) 366, 764, 1133, 1517
BtgI CCRYGG 2 cut(s) 1025, 1402
BtsCI GGATG 3 cut(s) 451, 877, 1646
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 6 cut(s) 298, 355, 425, 1038, 1231, 1239
Cac8I GCNNGC 5 cut(s) 204, 488, 1201, 1592, 1695
CaiI CAGNNNCTG 2 cut(s) 80, 923
CciI TCATGA 1 cut(s) 1000
Cfr13I GGNCC 6 cut(s) 364, 763, 914, 1013, 1399, 1516
CseI GACGC 2 cut(s) 126, 729
Csp6I GTAC 4 cut(s) 559, 802, 1098, 1700
CspCI CAANNNNNGTGG 2 cut(s) 1360, 1395
CviAII CATG 8 cut(s) 98, 378, 1001, 1026, 1204, 1394, 1555, 1795
CviQI GTAC 4 cut(s) 559, 802, 1098, 1700
DdeI CTNAG 6 cut(s) 238, 347, 840, 1111, 1283, 1424
DpnI GATC 9 cut(s) 48, 267, 586, 751, 1005, 1023, 1529, 1646, 1656
DpnII GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
Eam1104I CTCTTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 446
Eco130I CCWWGG 2 cut(s) 132, 1025
Eco24I GRGCYC 1 cut(s) 1335
Eco31I GGTCTC 1 cut(s) 678
Eco47I GGWCC 3 cut(s) 914, 1013, 1399
Eco57I CTGAAG 2 cut(s) 131, 1491
Eco72I CACGTG 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 1516
EcoRI GAATTC 1 cut(s) 1499
EcoRII CCWGG 2 cut(s) 790, 1636
EcoT14I CCWWGG 2 cut(s) 132, 1025
EcoT22I ATGCAT 2 cut(s) 1556, 1757
EcoT38I GRGCYC 1 cut(s) 1335
ErhI CCWWGG 2 cut(s) 132, 1025
FaeI CATG 8 cut(s) 101, 381, 1004, 1029, 1207, 1397, 1558, 1798
FaqI GGGAC 3 cut(s) 130, 799, 908
FatI CATG 8 cut(s) 97, 377, 1000, 1025, 1203, 1393, 1554, 1794
FauNDI CATATG 2 cut(s) 1220, 1671
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 9 cut(s) 285, 367, 855, 858, 984, 1197, 1362, 1588, 1707
FokI GGATG 3 cut(s) 438, 884, 1653
FriOI GRGCYC 1 cut(s) 1335
Fsp4HI GCNGC 9 cut(s) 285, 367, 855, 858, 984, 1197, 1362, 1588, 1707
FspBI CTAG 9 cut(s) 188, 203, 212, 479, 861, 1200, 1595, 1623, 1703
GluI GCNGC 9 cut(s) 285, 367, 855, 858, 984, 1197, 1362, 1588, 1707
GsaI CCCAGC 1 cut(s) 1196
HaeIII GGCC 4 cut(s) 366, 764, 1133, 1517
HgaI GACGC 2 cut(s) 126, 729
Hin1I GRCGYC 3 cut(s) 137, 431, 740
Hin1II CATG 8 cut(s) 101, 381, 1004, 1029, 1207, 1397, 1558, 1798
HincII GTYRAC 1 cut(s) 1493
HindII GTYRAC 1 cut(s) 1493
HindIII AAGCTT 1 cut(s) 1534
HinfI GANTC 7 cut(s) 14, 71, 193, 353, 920, 1326, 1769
HphI GGTGA 4 cut(s) 52, 1178, 1418, 1618
Hpy166II GTNNAC 4 cut(s) 235, 306, 1493, 1547
Hpy188I TCNGA 9 cut(s) 76, 149, 584, 589, 932, 1171, 1343, 1471, 1774
Hpy188III TCNNGA 6 cut(s) 50, 250, 263, 269, 682, 1001
Hpy8I GTNNAC 4 cut(s) 235, 306, 1493, 1547
HpyAV CCTTC 6 cut(s) 106, 465, 589, 891, 905, 1774
HpyCH4III ACNGT 6 cut(s) 10, 293, 316, 813, 956, 1042
HpyCH4IV ACGT 4 cut(s) 431, 832, 1009, 1728
HpyF10VI GCNNNNNNNGC 8 cut(s) 162, 212, 284, 629, 761, 1367, 1425, 1703
HpyF3I CTNAG 6 cut(s) 238, 347, 840, 1111, 1283, 1424
HpySE526I ACGT 4 cut(s) 431, 832, 1009, 1728
Hsp92I GRCGYC 3 cut(s) 137, 431, 740
Hsp92II CATG 8 cut(s) 101, 381, 1004, 1029, 1207, 1397, 1558, 1798
KpnI GGTACC 1 cut(s) 562
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 2 cut(s) 332, 1338
Lsp1109I GCAGC 8 cut(s) 271, 841, 844, 995, 1183, 1373, 1599, 1718
MaeI CTAG 9 cut(s) 188, 203, 212, 479, 861, 1200, 1595, 1623, 1703
MaeII ACGT 4 cut(s) 431, 832, 1009, 1728
MaeIII GTNAC 2 cut(s) 350, 1042
MalI GATC 9 cut(s) 48, 267, 586, 751, 1005, 1023, 1529, 1646, 1656
MboI GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
MboII GAAGA 6 cut(s) 433, 1067, 1111, 1423, 1495, 1820
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 749, 1644
MhlI GDGCHC 4 cut(s) 158, 329, 1335, 1821
MlyI GAGTC 2 cut(s) 347, 1763
MmeI TCCRAC 1 cut(s) 1194
Mph1103I ATGCAT 2 cut(s) 1556, 1757
MseI TTAA 4 cut(s) 374, 806, 1304, 1758
MslI CAYNNNNRTG 3 cut(s) 102, 957, 1127
MspA1I CMGCKG 2 cut(s) 1196, 1364
MspR9I CCNGG 2 cut(s) 792, 1638
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 2 cut(s) 792, 1638
MwoI GCNNNNNNNGC 8 cut(s) 162, 212, 284, 629, 761, 1367, 1425, 1703
NcoI CCATGG 1 cut(s) 1025
NdeI CATATG 2 cut(s) 1220, 1671
NdeII GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
NheI GCTAGC 2 cut(s) 202, 1199
NlaIII CATG 8 cut(s) 101, 381, 1004, 1029, 1207, 1397, 1558, 1798
NlaIV GGNNCC 6 cut(s) 365, 560, 1334, 1369, 1518, 1646
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 2 cut(s) 1556, 1757
NspI RCATGY 3 cut(s) 381, 1207, 1397
NspV TTCGAA 1 cut(s) 626
PagI TCATGA 1 cut(s) 1000
PciI ACATGT 2 cut(s) 377, 1393
PciSI GCTCTTC 1 cut(s) 446
PctI GAATGC 1 cut(s) 280
PfeI GAWTC 5 cut(s) 14, 71, 193, 920, 1326
PkrI GCNGC 9 cut(s) 286, 368, 856, 859, 985, 1198, 1363, 1589, 1708
PleI GAGTC 2 cut(s) 347, 1763
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
PpsI GAGTC 2 cut(s) 347, 1763
Ppu21I YACGTR 3 cut(s) 833, 1010, 1729
PscI ACATGT 2 cut(s) 377, 1393
PsiI TTATAA 1 cut(s) 1601
Psp6I CCWGG 2 cut(s) 790, 1636
PspCI CACGTG 1 cut(s) 833
PspFI CCCAGC 1 cut(s) 1192
PspGI CCWGG 2 cut(s) 790, 1636
PspN4I GGNNCC 6 cut(s) 365, 560, 1334, 1369, 1518, 1646
PspPI GGNCC 6 cut(s) 364, 763, 914, 1013, 1399, 1516
PstNI CAGNNNCTG 2 cut(s) 80, 923
PsuI RGATCY 2 cut(s) 749, 1644
PvuII CAGCTG 2 cut(s) 1196, 1364
RsaI GTAC 4 cut(s) 560, 803, 1099, 1701
RsaNI GTAC 4 cut(s) 559, 802, 1098, 1700
RseI CAYNNNNRTG 3 cut(s) 102, 957, 1127
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 4 cut(s) 374, 806, 1304, 1758
SatI GCNGC 9 cut(s) 285, 367, 855, 858, 984, 1197, 1362, 1588, 1707
Sau3AI GATC 9 cut(s) 46, 265, 584, 749, 1003, 1021, 1527, 1644, 1654
Sau96I GGNCC 6 cut(s) 364, 763, 914, 1013, 1399, 1516
ScaI AGTACT 1 cut(s) 1701
SchI GAGTC 2 cut(s) 347, 1763
ScrFI CCNGG 2 cut(s) 792, 1638
SduI GDGCHC 4 cut(s) 158, 329, 1335, 1821
SfcI CTRYAG 1 cut(s) 570
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 3 cut(s) 914, 1013, 1399
SmiMI CAYNNNNRTG 3 cut(s) 102, 957, 1127
SmlI CTYRAG 1 cut(s) 1047
SmoI CTYRAG 1 cut(s) 1047
SpeI ACTAGT 1 cut(s) 478
SsiI CCGC 2 cut(s) 367, 1287
SspMI CTAG 9 cut(s) 188, 203, 212, 479, 861, 1200, 1595, 1623, 1703
StyD4I CCNGG 2 cut(s) 790, 1636
StyI CCWWGG 2 cut(s) 132, 1025
TaaI ACNGT 6 cut(s) 10, 293, 316, 813, 956, 1042
TaiI ACGT 4 cut(s) 434, 835, 1012, 1731
TaqI TCGA 2 cut(s) 626, 683
TatI WGTACW 1 cut(s) 1699
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 5 cut(s) 14, 71, 193, 920, 1326
Tru1I TTAA 4 cut(s) 374, 806, 1304, 1758
Tru9I TTAA 4 cut(s) 374, 806, 1304, 1758
TscAI CASTG 6 cut(s) 298, 355, 432, 1045, 1231, 1246
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 8 cut(s) 284, 854, 857, 983, 1196, 1361, 1587, 1706
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 5 cut(s) 114, 120, 918, 1088, 1178
TspGWI ACGGA 1 cut(s) 1391
TspRI CASTG 6 cut(s) 298, 355, 432, 1045, 1231, 1246
VpaK11BI GGWCC 3 cut(s) 914, 1013, 1399
XapI RAATTY 3 cut(s) 1440, 1499, 1785
XceI RCATGY 3 cut(s) 381, 1207, 1397
XcmI CCANNNNNNNNNTGG 1 cut(s) 1023
XspI CTAG 9 cut(s) 188, 203, 212, 479, 861, 1200, 1595, 1623, 1703
ZraI GACGTC 1 cut(s) 432
ZrmI AGTACT 1 cut(s) 1701
Zsp2I ATGCAT 2 cut(s) 1556, 1757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.