Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0007279

Physical Location & Seq

Genomic Coordinates Forward (+)

223594 .. 224962

Gene Structure 1369 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g00220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGGAATTGGAAGCTGATGTTGGATTTCTGGATGAAATCGATTGGGATCTTTGGTTAGAGGACGCCTCTATTTCCAATGGATTGGATCATGTCTTTCAGTTGCCAAATCCACCCGTCACGGCGACCGGTTCAACGCCGCTGCCGCCATCATCATCCTCTTCGCCATCTTCAACGGTGGAGGAGCCTGACCCTCCCTCCAACCCATCCCCGGATTTTCTCGGCGCGATCGAGACCATTTTGATGAAAGACGACGATTTCGACGCCAACAAGGTTCCTTTGGCGGAATCGGGACCCGCTAACAACGAGTACTACGACCAATTTTTGGCAGATATACTCGTCAACTCGCCTTCCACTGACCGCGATTCCAACGCTTTCCCCGATTCCGACAAAGACAAATTCGACCGCTCGCCGCTCACAGATGACGCCGACGAAGATCCTATCTCCAAGAAAAGGAGAAGGCAGTTGAGAAACAAGGATGCGGCCGTGAGATCAAGGGAGAGGAGAAAGATGTATGTGAAGGATCTGGAGATTAAGAGCAAGTATTTTGAAGCTGAATGCAGGAGACTGGGGAATCTGCTCCAGTGCTGCTACGCCGAGAATCATGCTTTACGGTTGAGTTTGCAGATGAACAACACATATCGACATGGACATGGTGGTTTGGCCTCAAAGCAGGAGTCTGCTGTGCTCTTTTTGGAACTCCTGCTGTTGGGTTCCCTGCTTTGGTGCCTGGACATCATGTGCCTCCTTGCTCTGCCTCGAACTCTAATGACCCTGCTGTTACATCTACATCCACTGAAAAACGTGGACGTGGACGGAAACAAAGCTCTAGGAAGTGTGGGTCAAAGGCCAAGAGGGGGGTCGAGTAAGATGTTTCAACTCCGATCTTTTTCTAAAAGTAGGAGATGCAAATCGTCAAGGACAAAGATGAAACAGAGTGGGTTGCCTTGGTTCCTGAGTGTTTACGTGAACTCTACTCTCCCTCCTCTAATGTTAATAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0009889 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0023052 GO:0030968 GO:0031323 GO:0031326 GO:0031984 GO:0033554 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0042175 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0080090 GO:0098827 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

bZIP_1 bZIP_2

Protein Analysis

334

Amino Acids

37.29

Weight (kDa)

5.62

Isoelectric Point (pI)

66.03

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42990
fragaria_vesca	FvH4_5g26440
malus_domestica	MD06G1002100.v1.1
prunus_persica	Prupe.5G003000_v2.0.a1
pyrus_communis	pycom06g00220
rosa_chinensis	RchiOBHm_Chr7g0217321
rosa_laevigata	RLG00000002495 RLG00000003279 RLG00000008982 RLG00000010053
rosa_multiflora	Rmu_co8478867.1_g000001 Rmu_sc0002399.1_g000009 Rmu_sc0002842.1_g000001 Rmu_sc0007784.1_g000005 Rmu_sc0007843.1_g000003
rosa_roxburghii	Rroxscaffold_3G00242450
rosa_rugosa	Rorug02G0159300 Rorug07G0168000
rosa_samantha	Rh7AG307200 Rh7BG298100 Rh7CG185700 Rh7CG326200 Rh7DG306800
rosa_wichuraiana	Rw7G026080 Rw7G029750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	723
AccB7I	CCANNNNNTGG	1 cut(s)	322
AccBSI	CCGCTC	2 cut(s)	405, 412
AccII	CGCG	2 cut(s)	224, 360
AciI	CCGC	8 cut(s)	137, 143, 281, 294, 358, 403, 410, 479
AclWI	GGATC	4 cut(s)	54, 93, 428, 528
AcoI	YGGCCR	1 cut(s)	480
AcsI	RAATTY	1 cut(s)	395
AcyI	GRCGYC	3 cut(s)	63, 261, 423
AfaI	GTAC	1 cut(s)	308
AfiI	CCNNNNNNNGG	6 cut(s)	208, 322, 450, 706, 720, 854
AgeI	ACCGGT	1 cut(s)	125
AgsI	TTSAA	4 cut(s)	132, 171, 548, 875
AjiI	CACGTC	1 cut(s)	808
AjnI	CCWGG	1 cut(s)	726
AjuI	GAANNNNNNNTTGG	1 cut(s)	35
AluBI	AGCT	3 cut(s)	14, 551, 824
AluI	AGCT	3 cut(s)	14, 551, 824
Alw21I	GWGCWC	1 cut(s)	687
Alw26I	GTCTC	2 cut(s)	224, 556
AlwI	GGATC	4 cut(s)	54, 93, 428, 528
AoxI	GGCC	3 cut(s)	480, 660, 845
ApeKI	GCWGC	2 cut(s)	139, 585
ApoI	RAATTY	1 cut(s)	395
ArsI	GACNNNNNNTTYG	4 cut(s)	239, 271, 659, 691
AsiGI	ACCGGT	1 cut(s)	125
AspLEI	GCGC	1 cut(s)	224
AspS9I	GGNCC	1 cut(s)	290
AsuC2I	CCSGG	1 cut(s)	209
AvaII	GGWCC	1 cut(s)	290
BanI	GGYRCC	1 cut(s)	723
Bbv12I	GWGCWC	1 cut(s)	687
BbvI	GCAGC	2 cut(s)	126, 572
BccI	CCATC	3 cut(s)	154, 172, 211
BceAI	ACGGC	2 cut(s)	135, 467
BcgI	CGANNNNNNTGC	2 cut(s)	584, 618
BciT130I	CCWGG	1 cut(s)	728
BcnI	CCSGG	1 cut(s)	209
BcoDI	GTCTC	2 cut(s)	224, 556
BfaI	CTAG	1 cut(s)	827
BisI	GCNGC	6 cut(s)	137, 140, 143, 410, 480, 586
BlsI	GCNGC	6 cut(s)	138, 141, 144, 411, 481, 587
BmcAI	AGTACT	1 cut(s)	308
Bme1390I	CCNGG	2 cut(s)	209, 728
Bme18I	GGWCC	1 cut(s)	290
BmgBI	CACGTC	1 cut(s)	808
BmgT120I	GGNCC	1 cut(s)	290
BmiI	GGNNCC	7 cut(s)	183, 273, 291, 292, 712, 725, 950
BmrFI	CCNGG	2 cut(s)	209, 728
BmrI	ACTGGG	1 cut(s)	575
BmsI	GCATC	2 cut(s)	466, 893
BmuI	ACTGGG	1 cut(s)	575
BplI	GAGNNNNNCTC	2 cut(s)	50, 82
BpmI	CTGGAG	2 cut(s)	545, 563
BpuMI	CCSGG	1 cut(s)	209
Bsa29I	ATCGAT	1 cut(s)	39
BsaAI	YACGTR	1 cut(s)	964
BsaBI	GATNNNNATC	2 cut(s)	45, 907
BsaHI	GRCGYC	3 cut(s)	63, 261, 423
BsaI	GGTCTC	1 cut(s)	224
BsaJI	CCNNGG	2 cut(s)	207, 944
BsaWI	WCCGGW	1 cut(s)	125
BsaXI	ACNNNNNCTCC	4 cut(s)	179, 209, 964, 994
Bsc4I	CCNNNNNNNGG	6 cut(s)	208, 322, 450, 706, 720, 854
Bse118I	RCCGGY	1 cut(s)	125
Bse1I	ACTGG	2 cut(s)	570, 580
Bse8I	GATNNNNATC	2 cut(s)	45, 907
BseBI	CCWGG	1 cut(s)	728
BseCI	ATCGAT	1 cut(s)	39
BseDI	CCNNGG	2 cut(s)	207, 944
BseGI	GGATG	5 cut(s)	37, 152, 203, 481, 787
BseJI	GATNNNNATC	2 cut(s)	45, 907
BseLI	CCNNNNNNNGG	6 cut(s)	208, 322, 450, 706, 720, 854
BseMII	CTCAG	1 cut(s)	944
BseNI	ACTGG	2 cut(s)	570, 580
BseRI	GAGGAG	3 cut(s)	194, 514, 972
BseX3I	CGGCCG	1 cut(s)	480
BseXI	GCAGC	2 cut(s)	126, 572
Bsh1236I	CGCG	2 cut(s)	224, 360
Bsh1285I	CGRYCG	4 cut(s)	126, 228, 403, 483
BshFI	GGCC	3 cut(s)	482, 662, 847
BshNI	GGYRCC	1 cut(s)	723
BshTI	ACCGGT	1 cut(s)	125
BshVI	ATCGAT	1 cut(s)	39
BsiEI	CGRYCG	4 cut(s)	126, 228, 403, 483
BsiHKAI	GWGCWC	1 cut(s)	687
BsiSI	CCGG	2 cut(s)	126, 209
BslFI	GGGAC	1 cut(s)	303
BslI	CCNNNNNNNGG	6 cut(s)	208, 322, 450, 706, 720, 854
BsmAI	GTCTC	2 cut(s)	224, 556
BsmFI	GGGAC	1 cut(s)	303
BsmI	GAATGC	1 cut(s)	560
BsnI	GGCC	3 cut(s)	482, 662, 847
Bso31I	GGTCTC	1 cut(s)	224
Bsp1286I	GDGCHC	1 cut(s)	687
Bsp143I	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
BspACI	CCGC	8 cut(s)	137, 143, 281, 294, 358, 403, 410, 479
BspANI	GGCC	3 cut(s)	482, 662, 847
BspCNI	CTCAG	1 cut(s)	945
BspDI	ATCGAT	1 cut(s)	39
BspFNI	CGCG	2 cut(s)	224, 360
BspLI	GGNNCC	7 cut(s)	183, 273, 291, 292, 712, 725, 950
BspPI	GGATC	4 cut(s)	54, 93, 428, 528
BspT107I	GGYRCC	1 cut(s)	723
BspTNI	GGTCTC	1 cut(s)	224
BsrBI	CCGCTC	2 cut(s)	405, 412
BsrFI	RCCGGY	1 cut(s)	125
BsrI	ACTGG	2 cut(s)	570, 580
BssAI	RCCGGY	1 cut(s)	125
BssECI	CCNNGG	2 cut(s)	207, 944
BssMI	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
BssNI	GRCGYC	3 cut(s)	63, 261, 423
BssT1I	CCWWGG	1 cut(s)	944
Bst2UI	CCWGG	1 cut(s)	728
Bst4CI	ACNGT	2 cut(s)	175, 612
Bst6I	CTCTTC	1 cut(s)	163
BstACI	GRCGYC	3 cut(s)	63, 261, 423
BstBAI	YACGTR	1 cut(s)	964
BstC8I	GCNNGC	1 cut(s)	407
BstDEI	CTNAG	1 cut(s)	953
BstF5I	GGATG	5 cut(s)	37, 152, 203, 481, 787
BstFNI	CGCG	2 cut(s)	224, 360
BstHHI	GCGC	1 cut(s)	224
BstKTI	GATC	7 cut(s)	49, 88, 228, 436, 491, 523, 884
BstMAI	GTCTC	2 cut(s)	224, 556
BstMBI	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
BstMCI	CGRYCG	4 cut(s)	126, 228, 403, 483
BstMWI	GCNNNNNNNGC	1 cut(s)	142
BstNI	CCWGG	1 cut(s)	728
BstSCI	CCNGG	2 cut(s)	207, 726
BstUI	CGCG	2 cut(s)	224, 360
BstV1I	GCAGC	2 cut(s)	126, 572
BstX2I	RGATCY	3 cut(s)	46, 433, 520
BstXI	CCANNNNNNTGG	1 cut(s)	82
BstYI	RGATCY	3 cut(s)	46, 433, 520
BstZI	CGGCCG	1 cut(s)	480
Bsu15I	ATCGAT	1 cut(s)	39
BsuRI	GGCC	3 cut(s)	482, 662, 847
BsuTUI	ATCGAT	1 cut(s)	39
BtrI	CACGTC	1 cut(s)	808
BtsCI	GGATG	5 cut(s)	37, 152, 203, 481, 787
BtsIMutI	CAGTG	3 cut(s)	351, 587, 791
Cac8I	GCNNGC	1 cut(s)	407
CfoI	GCGC	1 cut(s)	224
Cfr10I	RCCGGY	1 cut(s)	125
Cfr13I	GGNCC	1 cut(s)	290
ClaI	ATCGAT	1 cut(s)	39
CseI	GACGC	3 cut(s)	71, 269, 431
Csp6I	GTAC	1 cut(s)	307
CspAI	ACCGGT	1 cut(s)	125
CviAII	CATG	5 cut(s)	89, 602, 644, 650, 736
CviJI	RGCY	7 cut(s)	14, 184, 482, 551, 662, 824, 847
CviKI_1	RGCY	7 cut(s)	14, 184, 482, 551, 662, 824, 847
CviQI	GTAC	1 cut(s)	307
DdeI	CTNAG	1 cut(s)	953
DpnI	GATC	7 cut(s)	48, 87, 227, 435, 490, 522, 883
DpnII	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
EaeI	YGGCCR	1 cut(s)	480
EagI	CGGCCG	1 cut(s)	480
Eam1104I	CTCTTC	1 cut(s)	163
EarI	CTCTTC	1 cut(s)	163
EciI	GGCGGA	1 cut(s)	296
EclXI	CGGCCG	1 cut(s)	480
Eco130I	CCWWGG	1 cut(s)	944
Eco31I	GGTCTC	1 cut(s)	224
Eco47I	GGWCC	1 cut(s)	290
Eco52I	CGGCCG	1 cut(s)	480
EcoO109I	RGGNCCY	1 cut(s)	290
EcoRII	CCWGG	1 cut(s)	726
EcoT14I	CCWWGG	1 cut(s)	944
ErhI	CCWWGG	1 cut(s)	944
FaeI	CATG	5 cut(s)	92, 605, 647, 653, 739
FaiI	YATR	8 cut(s)	90, 332, 513, 603, 637, 645, 651, 737
FaqI	GGGAC	1 cut(s)	303
FatI	CATG	5 cut(s)	88, 601, 643, 649, 735
FauI	CCCGC	1 cut(s)	301
Fnu4HI	GCNGC	6 cut(s)	137, 140, 143, 410, 480, 586
FokI	GGATG	5 cut(s)	44, 139, 190, 488, 774
Fsp4HI	GCNGC	6 cut(s)	137, 140, 143, 410, 480, 586
FspBI	CTAG	1 cut(s)	827
GlaI	GCGC	1 cut(s)	223
GluI	GCNGC	6 cut(s)	137, 140, 143, 410, 480, 586
GsuI	CTGGAG	2 cut(s)	545, 563
HaeIII	GGCC	3 cut(s)	482, 662, 847
HapII	CCGG	2 cut(s)	126, 209
HgaI	GACGC	3 cut(s)	71, 269, 431
HhaI	GCGC	1 cut(s)	224
Hin1I	GRCGYC	3 cut(s)	63, 261, 423
Hin1II	CATG	5 cut(s)	92, 605, 647, 653, 739
Hin6I	GCGC	1 cut(s)	222
HinP1I	GCGC	1 cut(s)	222
HincII	GTYRAC	1 cut(s)	340
HindII	GTYRAC	1 cut(s)	340
HinfI	GANTC	6 cut(s)	284, 362, 380, 571, 598, 674
HpaII	CCGG	2 cut(s)	126, 209
Hpy166II	GTNNAC	5 cut(s)	340, 805, 811, 961, 967
Hpy188I	TCNGA	2 cut(s)	385, 881
Hpy188III	TCNNGA	5 cut(s)	29, 229, 288, 524, 952
Hpy8I	GTNNAC	5 cut(s)	340, 805, 811, 961, 967
Hpy99I	CGWCG	3 cut(s)	254, 263, 431
HpyAV	CCTTC	3 cut(s)	357, 450, 511
HpyCH4III	ACNGT	2 cut(s)	175, 612
HpyCH4IV	ACGT	3 cut(s)	801, 807, 963
HpyCH4V	TGCA	3 cut(s)	558, 622, 906
HpyF10VI	GCNNNNNNNGC	1 cut(s)	142
HpyF3I	CTNAG	1 cut(s)	953
HpySE526I	ACGT	3 cut(s)	801, 807, 963
Hsp92I	GRCGYC	3 cut(s)	63, 261, 423
Hsp92II	CATG	5 cut(s)	92, 605, 647, 653, 739
HspAI	GCGC	1 cut(s)	222
KflI	GGGWCCC	1 cut(s)	290
Kzo9I	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
LmnI	GCTCC	2 cut(s)	181, 582
Lsp1109I	GCAGC	2 cut(s)	126, 572
LweI	GCATC	2 cut(s)	466, 893
MaeI	CTAG	1 cut(s)	827
MaeII	ACGT	3 cut(s)	801, 807, 963
MaeIII	GTNAC	2 cut(s)	115, 777
MalI	GATC	7 cut(s)	48, 87, 227, 435, 490, 522, 883
MbiI	CCGCTC	2 cut(s)	405, 412
MboI	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
MboII	GAAGA	3 cut(s)	150, 159, 443
MflI	RGATCY	3 cut(s)	46, 433, 520
MhlI	GDGCHC	1 cut(s)	687
MluCI	AATT	3 cut(s)	5, 317, 395
MlyI	GAGTC	1 cut(s)	683
MmeI	TCCRAC	3 cut(s)	222, 390, 408
MseI	TTAA	3 cut(s)	531, 992, 1000
MslI	CAYNNNNRTG	2 cut(s)	239, 648
MspA1I	CMGCKG	1 cut(s)	139
MspI	CCGG	2 cut(s)	126, 209
MspR9I	CCNGG	2 cut(s)	209, 728
Mva1269I	GAATGC	1 cut(s)	560
MvaI	CCWGG	1 cut(s)	728
MvnI	CGCG	2 cut(s)	224, 360
MwoI	GCNNNNNNNGC	1 cut(s)	142
NciI	CCSGG	1 cut(s)	209
NdeII	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
NlaIII	CATG	5 cut(s)	92, 605, 647, 653, 739
NlaIV	GGNNCC	7 cut(s)	183, 273, 291, 292, 712, 725, 950
NmeAIII	GCCGAG	2 cut(s)	198, 619
NmuCI	GTSAC	1 cut(s)	115
PcsI	WCGNNNNNNNCGW	4 cut(s)	225, 255, 309, 375
PctI	GAATGC	1 cut(s)	560
PfeI	GAWTC	5 cut(s)	284, 362, 380, 571, 598
PflMI	CCANNNNNTGG	1 cut(s)	322
PinAI	ACCGGT	1 cut(s)	125
PkrI	GCNGC	6 cut(s)	138, 141, 144, 411, 481, 587
Ple19I	CGATCG	1 cut(s)	228
PleI	GAGTC	1 cut(s)	682
PpsI	GAGTC	1 cut(s)	682
Ppu21I	YACGTR	1 cut(s)	964
PpuMI	RGGWCCY	1 cut(s)	290
Psp5II	RGGWCCY	1 cut(s)	290
Psp6I	CCWGG	1 cut(s)	726
PspGI	CCWGG	1 cut(s)	726
PspN4I	GGNNCC	7 cut(s)	183, 273, 291, 292, 712, 725, 950
PspPI	GGNCC	1 cut(s)	290
PspPPI	RGGWCCY	1 cut(s)	290
PsuI	RGATCY	3 cut(s)	46, 433, 520
PvuI	CGATCG	1 cut(s)	228
RsaI	GTAC	1 cut(s)	308
RsaNI	GTAC	1 cut(s)	307
RseI	CAYNNNNRTG	2 cut(s)	239, 648
SaqAI	TTAA	3 cut(s)	531, 992, 1000
SatI	GCNGC	6 cut(s)	137, 140, 143, 410, 480, 586
Sau3AI	GATC	7 cut(s)	46, 85, 225, 433, 488, 520, 881
Sau96I	GGNCC	1 cut(s)	290
ScaI	AGTACT	1 cut(s)	308
SchI	GAGTC	1 cut(s)	683
ScrFI	CCNGG	2 cut(s)	209, 728
SduI	GDGCHC	1 cut(s)	687
SetI	ASST	7 cut(s)	16, 273, 553, 804, 810, 826, 966
SfaNI	GCATC	2 cut(s)	466, 893
SinI	GGWCC	1 cut(s)	290
SmiMI	CAYNNNNRTG	2 cut(s)	239, 648
Sse9I	AATT	3 cut(s)	5, 317, 395
SsiI	CCGC	8 cut(s)	137, 143, 281, 294, 358, 403, 410, 479
SspMI	CTAG	1 cut(s)	827
StyD4I	CCNGG	2 cut(s)	207, 726
StyI	CCWWGG	1 cut(s)	944
TaaI	ACNGT	2 cut(s)	175, 612
TaiI	ACGT	3 cut(s)	804, 810, 966
TaqI	TCGA	7 cut(s)	39, 228, 258, 399, 640, 757, 860
TasI	AATT	3 cut(s)	5, 317, 395
TatI	WGTACW	1 cut(s)	306
TauI	GCSGC	4 cut(s)	139, 145, 412, 482
TfiI	GAWTC	5 cut(s)	284, 362, 380, 571, 598
Tru1I	TTAA	3 cut(s)	531, 992, 1000
Tru9I	TTAA	3 cut(s)	531, 992, 1000
TscAI	CASTG	3 cut(s)	358, 587, 798
TseFI	GTSAC	1 cut(s)	115
TseI	GCWGC	2 cut(s)	139, 585
Tsp45I	GTSAC	1 cut(s)	115
TspDTI	ATGAA	4 cut(s)	48, 257, 641, 941
TspGWI	ACGGA	1 cut(s)	828
TspRI	CASTG	3 cut(s)	358, 587, 798
Van91I	CCANNNNNTGG	1 cut(s)	322
VpaK11BI	GGWCC	1 cut(s)	290
XapI	RAATTY	1 cut(s)	395
XspI	CTAG	1 cut(s)	827
ZrmI	AGTACT	1 cut(s)	308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.