pycom07g01690
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003714
Physical Location & Seq
Forward (+)
1333584 .. 1337905
4322 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01690.1

Sequence Viewer

Length: 915 bp
ATGGGAAGGGGGAAGATTGAGATCAAGAGGATTGAGAATGCAAATAGCAGACAAGTCACATTCTCAAAGAGACGTTCTGGGTTGCTAAAAAAAGCTCAGGAATTGGCTATTCTCTGTGATGCTGAGGTTGCTGTTATTATCTTCTCCAATACTGGAAAGCTTTTTGAATTTTCCAGTGCTGGTATGATGCGAACTCTTTCAAGATACAACAAGTGTTTAGATTCTTCAGAAGATGCACCAGAAGAAGGCAAGGCAGAGATTCAGAAGCAAGACTGCAAGGAGCTGGATAAGCTAAAGGATGAATATGCAAAGCTACAAAAGAAACAATTAAGGCTGTTGGGTAAGGAGTTGACTGATTTGAGCTTGAAAGAATTGCAGCAACTAGAACAGCAATTGAATGAAGGATTACTGACAGTGAAGGAGAGGAAGGAGCAATTACTGACAGAACAACTAGAGCAATCAAGAATACAGGTTGAAGAGCTTCGATGTTTGTTTCCACAAACTGACAGTGTAGTCCAATCTTATTTGGAATATTATCCTGTGGAGAAAACTAAATCCCTTGTAAACCATGGCGTCACACATAGTCCTGATTTGGTCAGCAATTTTGCATTTGAGAACGGCGACTCTGACACCACCTTGCAGCTAGGGTTCAAGTTATGGACCCATGTTATGAAGAATACCATATTAGGGAAAATGACAAGTCACCCATTTCAGTTACTAGCCAAAGGCTATATATATGTCTTCACAGTGTACAAAATAATTAATTTTCAGAAATACAATTGGAAAGCTTACAGAGGAAGTTTCCAGTTTCTATATGGTATCACGCCATTTCTGCTTCACAGCATCGATCCTTCCGCTCCCTCAACTCGATCAATCGATCGATTCTTGATCGATCTTTCTGTTCTTCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003824 GO:0004601 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006355 GO:0006629 GO:0006720 GO:0006721 GO:0007275 GO:0008150 GO:0008152 GO:0008300 GO:0009056 GO:0009636 GO:0009685 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009900 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010033 GO:0010047 GO:0010154 GO:0010227 GO:0010262 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010817 GO:0016042 GO:0016054 GO:0016101 GO:0016103 GO:0016115 GO:0016209 GO:0016491 GO:0016684 GO:0019219 GO:0019222 GO:0019752 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0042221 GO:0042445 GO:0042447 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044464 GO:0045487 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046395 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048577 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048587 GO:0048608 GO:0048609 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051254 GO:0051716 GO:0055114 GO:0060255 GO:0060860 GO:0060862 GO:0060867 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0080050 GO:0080090 GO:0090567 GO:0097159 GO:0097237 GO:0098754 GO:0098869 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990748 GO:2000026 GO:2000028 GO:2000034 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2000692 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

305

Amino Acids

34.98

Weight (kDa)

8.67

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 4.8e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 161 1.5e-15 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 857
AciI CCGC 1 cut(s) 855
AclWI GGATC 1 cut(s) 842
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 245, 687
AgsI TTSAA 6 cut(s) 167, 201, 367, 397, 476, 652
AluBI AGCT 9 cut(s) 95, 160, 283, 292, 313, 363, 481, 643, 788
AluI AGCT 9 cut(s) 95, 160, 283, 292, 313, 363, 481, 643, 788
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 1 cut(s) 842
ApeKI GCWGC 2 cut(s) 376, 640
ApoI RAATTY 1 cut(s) 167
AseI ATTAAT 1 cut(s) 762
Asp700I GAANNNNTTC 2 cut(s) 196, 480
AspS9I GGNCC 1 cut(s) 660
AsuHPI GGTGA 1 cut(s) 695
AvaII GGWCC 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 388, 652
BceAI ACGGC 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 4 cut(s) 383, 452, 644, 719
BisI GCNGC 2 cut(s) 377, 641
BlsI GCNGC 2 cut(s) 378, 642
Bme18I GGWCC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 660
BmiI GGNNCC 1 cut(s) 662
BmsI GCATC 4 cut(s) 109, 177, 223, 852
BpiI GAAGAC 1 cut(s) 733
Bpu10I CCTNAGC 2 cut(s) 96, 123
Bsa29I ATCGAT 4 cut(s) 846, 876, 880, 891
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 687
Bse1I ACTGG 3 cut(s) 157, 174, 805
Bse8I GATNNNNATC 1 cut(s) 20
BseCI ATCGAT 4 cut(s) 846, 876, 880, 891
BseDI CCNNGG 1 cut(s) 568
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 245, 687
BseMII CTCAG 2 cut(s) 110, 114
BseNI ACTGG 3 cut(s) 157, 174, 805
BseRI GAGGAG 1 cut(s) 899
BseXI GCAGC 2 cut(s) 388, 652
Bsh1285I CGRYCG 1 cut(s) 880
BshVI ATCGAT 4 cut(s) 846, 876, 880, 891
BsiEI CGRYCG 1 cut(s) 880
BslI CCNNNNNNNGG 2 cut(s) 245, 687
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsmI GAATGC 1 cut(s) 43
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 6 cut(s) 21, 847, 869, 877, 888, 892
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 1 cut(s) 855
BspCNI CTCAG 2 cut(s) 109, 115
BspDI ATCGAT 4 cut(s) 846, 876, 880, 891
BspLI GGNNCC 1 cut(s) 662
BspPI GGATC 1 cut(s) 842
BspQI GCTCTTC 1 cut(s) 471
BsrBI CCGCTC 1 cut(s) 857
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 3 cut(s) 157, 174, 805
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 6 cut(s) 21, 847, 869, 877, 888, 892
BssNI GRCGYC 1 cut(s) 573
BssT1I CCWWGG 1 cut(s) 568
Bst4CI ACNGT 3 cut(s) 415, 509, 748
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 750
BstDEI CTNAG 2 cut(s) 96, 123
BstDSI CCRYGG 1 cut(s) 568
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 6 cut(s) 24, 850, 872, 880, 891, 895
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 6 cut(s) 21, 847, 869, 877, 888, 892
BstMCI CGRYCG 1 cut(s) 880
BstMWI GCNNNNNNNGC 3 cut(s) 128, 289, 832
BstV1I GCAGC 2 cut(s) 388, 652
BstV2I GAAGAC 1 cut(s) 733
Bsu15I ATCGAT 4 cut(s) 846, 876, 880, 891
BsuTUI ATCGAT 4 cut(s) 846, 876, 880, 891
BtgI CCRYGG 1 cut(s) 568
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 4 cut(s) 181, 420, 514, 753
Cfr13I GGNCC 1 cut(s) 660
ClaI ATCGAT 4 cut(s) 846, 876, 880, 891
CseI GACGC 1 cut(s) 562
Csp6I GTAC 1 cut(s) 751
CviAII CATG 2 cut(s) 569, 665
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 2 cut(s) 96, 123
DpnI GATC 6 cut(s) 23, 849, 871, 879, 890, 894
DpnII GATC 6 cut(s) 21, 847, 869, 877, 888, 892
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 2 cut(s) 572, 668
FatI CATG 2 cut(s) 568, 664
Fnu4HI GCNGC 2 cut(s) 377, 641
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 377, 641
FspBI CTAG 4 cut(s) 383, 452, 644, 719
GluI GCNGC 2 cut(s) 377, 641
HgaI GACGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 2 cut(s) 572, 668
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 2 cut(s) 158, 786
HinfI GANTC 4 cut(s) 221, 259, 623, 882
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 3 cut(s) 351, 565, 751
Hpy188I TCNGA 5 cut(s) 229, 264, 628, 771, 914
Hpy188III TCNNGA 6 cut(s) 25, 98, 201, 462, 587, 886
Hpy8I GTNNAC 3 cut(s) 351, 565, 751
HpyAV CCTTC 5 cut(s) 239, 395, 412, 421, 861
HpyCH4III ACNGT 3 cut(s) 415, 509, 748
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 7 cut(s) 41, 236, 276, 308, 376, 608, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 289, 832
HpyF3I CTNAG 2 cut(s) 96, 123
HpySE526I ACGT 1 cut(s) 73
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 2 cut(s) 572, 668
Kzo9I GATC 6 cut(s) 21, 847, 869, 877, 888, 892
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 3 cut(s) 280, 430, 862
Lsp1109I GCAGC 2 cut(s) 388, 652
LweI GCATC 4 cut(s) 109, 177, 223, 852
MaeI CTAG 4 cut(s) 383, 452, 644, 719
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 4 cut(s) 55, 574, 701, 714
MalI GATC 6 cut(s) 23, 849, 871, 879, 890, 894
MbiI CCGCTC 1 cut(s) 857
MboI GATC 6 cut(s) 21, 847, 869, 877, 888, 892
MboII GAAGA 9 cut(s) 25, 133, 216, 242, 254, 488, 685, 733, 896
MfeI CAATTG 2 cut(s) 392, 778
MlyI GAGTC 1 cut(s) 617
MnlI CCTC 5 cut(s) 21, 118, 417, 788, 871
MroXI GAANNNNTTC 2 cut(s) 196, 480
MseI TTAA 2 cut(s) 329, 762
MunI CAATTG 2 cut(s) 392, 778
Mva1269I GAATGC 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 128, 289, 832
NcoI CCATGG 1 cut(s) 568
NdeII GATC 6 cut(s) 21, 847, 869, 877, 888, 892
NlaIII CATG 2 cut(s) 572, 668
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 3 cut(s) 55, 574, 701
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 1 cut(s) 43
PdmI GAANNNNTTC 2 cut(s) 196, 480
PfeI GAWTC 3 cut(s) 221, 259, 882
PkrI GCNGC 2 cut(s) 378, 642
Ple19I CGATCG 1 cut(s) 880
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
PshBI ATTAAT 1 cut(s) 762
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 1 cut(s) 660
PvuI CGATCG 1 cut(s) 880
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 329, 762
SatI GCNGC 2 cut(s) 377, 641
Sau3AI GATC 6 cut(s) 21, 847, 869, 877, 888, 892
Sau96I GGNCC 1 cut(s) 660
SchI GAGTC 1 cut(s) 617
SfaNI GCATC 4 cut(s) 109, 177, 223, 852
SinI GGWCC 1 cut(s) 660
SsiI CCGC 1 cut(s) 855
SspI AATATT 1 cut(s) 533
SspMI CTAG 4 cut(s) 383, 452, 644, 719
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 3 cut(s) 415, 509, 748
TaiI ACGT 1 cut(s) 76
TaqI TCGA 6 cut(s) 484, 846, 868, 876, 880, 891
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 3 cut(s) 221, 259, 882
Tru1I TTAA 2 cut(s) 329, 762
Tru9I TTAA 2 cut(s) 329, 762
TscAI CASTG 4 cut(s) 181, 420, 514, 753
TseFI GTSAC 3 cut(s) 55, 574, 701
TseI GCWGC 2 cut(s) 376, 640
Tsp45I GTSAC 3 cut(s) 55, 574, 701
TspDTI ATGAA 3 cut(s) 315, 414, 686
TspRI CASTG 4 cut(s) 181, 420, 514, 753
VpaK11BI GGWCC 1 cut(s) 660
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 812
XmnI GAANNNNTTC 2 cut(s) 196, 480
XspI CTAG 4 cut(s) 383, 452, 644, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.