Rroxscaffold_4G00329440
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003714
Physical Location & Seq
Reverse (-)
62763795 .. 62770011
6217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329440.1

Sequence Viewer

Length: 717 bp
ATGAAGAAAAAAATGGGTAGGGGGAAGATTGAGATCAAGAAGATTGAGAACACCAATAGCAGACAAGTCACATTCTCAAAGAGGCGTGCTGGGTTACTCAAGAAAGCTCATGAATTGGCTATTCTCTGCGATGCTGAGGTTGCTGTCATCGTCTTCTCTAACACTGGCAAGCTTTTTGAGTTTTCCAGTGCTGGTATGAAGCGAACTATTGCAAGATACAAGAAGTGTTATGAGTCTTCAGAGACCGCTCTGGTAGTATCAAGGGCAGAGGGGTTTGATTCCCTTGTAATTGAGGAGGACGACCCTAAGGACGTGGATGTTCTAAAAGATGAACTTGAGAAGCTACAACAGAATCAATTGCGTCTGTTGGGCAATGACTTGTCTAGTTTGAGCTTGACAGAATTGCAGAAACTAGAAAATCAATTAACTGAAGGATTATTTTCAGTGAAGGAGAAAAAGGTTGAGGAGCTTCGGCGTTTATTTCCCCAAGCTGATCATGCAGTTCCATCTTATCTCGACTATTGTCCTGTAGAAAAACAGAACTCCCCTGTGTACCATGGTGCCAAAAGCCCCGATTTGGTCAGCAATTTCGCATTTGACAATGGAGATTCTGACACTACATTGCAATTAGGGCTGCCAAGCGATACATATCGCAAAAGAAAGGCTCCAGAAAGAGAAAGCCACTCTAATGACTCAGGGAGCCAACTAGGCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003824 GO:0004601 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006355 GO:0006629 GO:0006720 GO:0006721 GO:0007275 GO:0008150 GO:0008152 GO:0008300 GO:0009056 GO:0009636 GO:0009685 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009900 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010033 GO:0010047 GO:0010154 GO:0010227 GO:0010262 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010817 GO:0016042 GO:0016054 GO:0016101 GO:0016103 GO:0016115 GO:0016209 GO:0016491 GO:0016684 GO:0019219 GO:0019222 GO:0019752 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0042221 GO:0042445 GO:0042447 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044464 GO:0045487 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046395 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048577 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048587 GO:0048608 GO:0048609 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051254 GO:0051716 GO:0055114 GO:0060255 GO:0060860 GO:0060862 GO:0060867 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0080050 GO:0080090 GO:0090567 GO:0097159 GO:0097237 GO:0098754 GO:0098869 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990748 GO:2000026 GO:2000028 GO:2000034 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2000692 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

238

Amino Acids

26.74

Weight (kDa)

6.92

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 14 - 61 7.7e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 103 - 155 6.9e-12 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 560
AccBSI CCGCTC 1 cut(s) 248
AciI CCGC 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 222, 450
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 577
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 6 cut(s) 107, 172, 343, 393, 469, 491
AluI AGCT 6 cut(s) 107, 172, 343, 393, 469, 491
Alw26I GTCTC 1 cut(s) 236
AoxI GGCC 1 cut(s) 709
ApeKI GCWGC 1 cut(s) 634
AxyI CCTNAGG 1 cut(s) 306
BanI GGYRCC 1 cut(s) 560
BbsI GAAGAC 2 cut(s) 145, 228
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 621
BccI CCATC 1 cut(s) 514
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 3 cut(s) 384, 413, 707
BfmI CTRYAG 1 cut(s) 528
BglI GCCNNNNNGGC 1 cut(s) 708
BisI GCNGC 1 cut(s) 635
BlsI GCNGC 1 cut(s) 636
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 3 cut(s) 562, 666, 701
BmsI GCATC 1 cut(s) 121
BoxI GACNNNNGTC 1 cut(s) 522
BpiI GAAGAC 2 cut(s) 145, 228
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 83, 356
BsaBI GATNNNNATC 2 cut(s) 32, 648
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 556
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 169, 186
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 2 cut(s) 379, 620
Bse8I GATNNNNATC 2 cut(s) 32, 648
BseDI CCNNGG 1 cut(s) 556
BseGI GGATG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 32, 648
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMI GCAATG 2 cut(s) 379, 620
BseMII CTCAG 2 cut(s) 126, 708
BseNI ACTGG 2 cut(s) 169, 186
BseRI GAGGAG 2 cut(s) 308, 479
BseXI GCAGC 1 cut(s) 621
BseYI CCCAGC 1 cut(s) 89
BshFI GGCC 1 cut(s) 711
BshNI GGYRCC 1 cut(s) 560
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 1 cut(s) 711
Bso31I GGTCTC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 33, 493
Bsp19I CCATGG 1 cut(s) 556
BspACI CCGC 1 cut(s) 246
BspANI GGCC 1 cut(s) 711
BspCNI CTCAG 2 cut(s) 127, 707
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 562, 666, 701
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 2 cut(s) 379, 620
BsrI ACTGG 2 cut(s) 169, 186
BssECI CCNNGG 1 cut(s) 556
BssMI GATC 2 cut(s) 33, 493
BssT1I CCWWGG 1 cut(s) 556
BstC8I GCNNGC 2 cut(s) 87, 170
BstDEI CTNAG 3 cut(s) 135, 306, 694
BstDSI CCRYGG 1 cut(s) 556
BstF5I GGATG 1 cut(s) 322
BstKTI GATC 2 cut(s) 36, 496
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 33, 493
BstMWI GCNNNNNNNGC 4 cut(s) 140, 497, 631, 708
BstPAI GACNNNNGTC 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 621
BstV2I GAAGAC 2 cut(s) 145, 228
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 556
BtgZI GCGATG 1 cut(s) 144
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 162, 193, 450
Cac8I GCNNGC 2 cut(s) 87, 170
CciI TCATGA 1 cut(s) 109
CseI GACGC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 553
CviAII CATG 3 cut(s) 110, 497, 557
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 3 cut(s) 135, 306, 694
DpnI GATC 2 cut(s) 35, 495
DpnII GATC 2 cut(s) 33, 493
Eco130I CCWWGG 1 cut(s) 556
Eco147I AGGCCT 1 cut(s) 711
Eco31I GGTCTC 1 cut(s) 236
Eco57I CTGAAG 2 cut(s) 222, 450
Eco81I CCTNAGG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 3 cut(s) 113, 500, 560
FaiI YATR 6 cut(s) 111, 197, 231, 498, 558, 649
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 3 cut(s) 109, 496, 556
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 635
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 1 cut(s) 635
FspBI CTAG 3 cut(s) 384, 413, 707
GluI GCNGC 1 cut(s) 635
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 1 cut(s) 711
HgaI GACGC 1 cut(s) 350
Hin1II CATG 3 cut(s) 113, 500, 560
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 5 cut(s) 233, 278, 352, 608, 692
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 2 cut(s) 241, 613
Hpy188III TCNNGA 5 cut(s) 37, 100, 110, 515, 668
Hpy8I GTNNAC 1 cut(s) 553
HpyAV CCTTC 2 cut(s) 425, 442
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 4 cut(s) 212, 406, 500, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 497, 631, 708
HpyF3I CTNAG 3 cut(s) 135, 306, 694
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 113, 500, 560
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 2 cut(s) 33, 493
LmnI GCTCC 3 cut(s) 466, 670, 699
LpnPI CCDG 9 cut(s) 75, 150, 177, 199, 236, 540, 561, 681, 681
Lsp1109I GCAGC 1 cut(s) 621
LweI GCATC 1 cut(s) 121
MaeI CTAG 3 cut(s) 384, 413, 707
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 67, 93
MalI GATC 2 cut(s) 35, 495
MbiI CCGCTC 1 cut(s) 248
MboI GATC 2 cut(s) 33, 493
MboII GAAGA 5 cut(s) 16, 37, 52, 145, 228
MfeI CAATTG 1 cut(s) 356
MluCI AATT 7 cut(s) 113, 288, 356, 401, 422, 586, 626
MlyI GAGTC 2 cut(s) 242, 686
MnlI CCTC 6 cut(s) 75, 130, 262, 286, 289, 457
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 687
MunI CAATTG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 140, 497, 631, 708
NcoI CCATGG 1 cut(s) 556
NdeII GATC 2 cut(s) 33, 493
NlaIII CATG 3 cut(s) 113, 500, 560
NlaIV GGNNCC 3 cut(s) 562, 666, 701
NmuCI GTSAC 1 cut(s) 67
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 711
PfeI GAWTC 3 cut(s) 278, 352, 608
PkrI GCNGC 1 cut(s) 636
PleI GAGTC 2 cut(s) 241, 686
PpsI GAGTC 2 cut(s) 241, 686
PshAI GACNNNNGTC 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 89
PspN4I GGNNCC 3 cut(s) 562, 666, 701
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 687
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 1 cut(s) 635
Sau3AI GATC 2 cut(s) 33, 493
SchI GAGTC 2 cut(s) 242, 686
SetI ASST 9 cut(s) 109, 141, 174, 315, 345, 395, 462, 471, 493
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 98, 335
SmoI CTYRAG 2 cut(s) 98, 335
Sse9I AATT 7 cut(s) 113, 288, 356, 401, 422, 586, 626
SseBI AGGCCT 1 cut(s) 711
SsiI CCGC 1 cut(s) 246
SspMI CTAG 3 cut(s) 384, 413, 707
StuI AGGCCT 1 cut(s) 711
StyI CCWWGG 1 cut(s) 556
TaiI ACGT 1 cut(s) 315
TaqI TCGA 1 cut(s) 516
TasI AATT 7 cut(s) 113, 288, 356, 401, 422, 586, 626
TfiI GAWTC 3 cut(s) 278, 352, 608
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 3 cut(s) 169, 193, 450
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 1 cut(s) 634
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 17, 126, 212, 345
TspRI CASTG 3 cut(s) 169, 193, 450
XspI CTAG 3 cut(s) 384, 413, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.