pycom07g04250

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005893
Physical Location & Seq
Forward (+)
3676296 .. 3681130
4835 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1689 bp
ATGTTACCTCACTACAATTCCTTTCCTCGCCATTCCAACCGAGCTCACACAACTGGTAGCTTGGCTCCACCTCCTCACCAGATGCAACCTCAATTGGGTATCAGGAATCTCGAAATTCCAGTCCAATTTAGTAATTCTAATTCCATGATAGCTCAACCCAATTTCATGAATCTACCAAACAACCTTCTTCCTATGCAAAATAACCATCTGGGTATGCCTCAATTCGGTTCCGTTGGCCCTACTTCTCAACCGGCTCAGCCGAATGTTGGGGTTTTCGGTTCGCAAGGTATGCCTCAATTCTGCAATTTGGTGCAGCAAGTGAATCAATTGATGTCATCTCAAGTGCCTGGGCAAATATTTGGGCATAACATGTTAAATTTACAGCAACAGATGAACCAGAATATGGGTTTGCCGTATGGACAATTTTGTTTGCCGAATTCAATGCAAAACATGAATCAATTTGTGCCAATGCAAATGCAAAATCCATCGCAAGTCCATCCTAATTATGGTTTTCCGGGATCAAATCAAGCACCTCAGACCACAATGTCTCAAAATGTCCCATTGTTTGCAAATAACCAGGTTAATCTGCATTCTAATCAGGCAGGCCAGCAAGTTAACCAAAATCAGCAAAACTTGGGTCTACCTCCAGTGCAAGGGACACAGGGAAATAAAACAAATAGTAGTGGTGGTTACAATTCAAATTCCAATTGGAAACAATCACCAAGCAAAAGCGTTACAAAGAATCCGAAGAATCACCAAGGGGGATTTCAGAATCCTCAGTCGCATCATATGAAAAATGCAAAGGGAAAGTTCGCATTTCCTAATGGGCACAAAGGAAAAGGGTTGAGAAATGAAAGCGAAGGAAAGTTTGCTCTGGCCAACTCTTCAAACCAGGGCAGGGAAAGGAAGAGACCTCTTTTCTTGTCATATACGGAACAAGAGATTGAGCGGTGGCGGGAAGACCGTAGGAAGCACTACCCGTCGAAATCCAACATAGAGAAGAAGATAAGTGAAAAGCAGATAAATGTAGAGGTCATCGAAAGAGAGGCAAACATTCGACGAGAGCAACTCAAGGATATTCTTACAAAGCAGGCTGAGTTGGGAGTTCAAGTTGCTGAAGTACCATCGCACTACCTCGCAGATTCAAATCAAGGGCATATAAAGGAAGATAATGAAACATTGACCAAGAAGGGGAGGTCAACAAACAAGTTTGGTAAGAGAGAAAAATATGATAGAAAGGATCGGTTTTCCAAGAGGCAAAGATCACATCAAAAGGATTCGTCTAATGACCCTTCTTTCGACAAAAGGGAGCCAAACAAAAGGGAGCCAACCTTGCTGCAGAAGCTTCTTAGTGCAGATATTAGGAGAGACAGAAGCCACCTGCTGCAGGTGTTTAGGTTCATGGTGACAAACTCTTTCTTCAAAGATTGTCCTGACAAACCTTTGAAATTCCCTACTGTAGCAGTAAAAGAAAGTGCCGGTGACGGAGAGGTGGCTCAAAAGCCGTCCTCACTTATAGAGAAAGATGCTTTTAAGGGAAGTGATAAATCCATGGTTGGAAGAGTTTATAACAATCATTCTGGAAATAACAACGATGTCGAGGATTCTGTTGATAGCAGTGATGAAGATGACGGTGAAGGAGAGGGAACAGAGAGACCTGCGGAAGAAGAGGAAGAAATCATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

563

Amino Acids

63.33

Weight (kDa)

9.24

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 309 - 342 3.7e-10 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1569
AarI CACCTGC 2 cut(s) 1378, 1389
AasI GACNNNNNNGTC 1 cut(s) 544
Acc36I ACCTGC 3 cut(s) 1378, 1389, 1666
AccB7I CCANNNNNTGG 1 cut(s) 403
AccBSI CCGCTC 1 cut(s) 949
AccI GTMKAC 1 cut(s) 640
AciI CCGC 3 cut(s) 949, 955, 1661
AclWI GGATC 2 cut(s) 526, 1248
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 5 cut(s) 114, 376, 436, 700, 1448
AcuI CTGAAG 1 cut(s) 1137
AfaI GTAC 1 cut(s) 1122
AfiI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1191, 1387
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 7 cut(s) 441, 699, 888, 1109, 1146, 1423, 1447
AjnI CCWGG 3 cut(s) 346, 576, 891
AjuI GAANNNNNNNTTGG 2 cut(s) 750, 782
AluBI AGCT 4 cut(s) 44, 60, 152, 1345
AluI AGCT 4 cut(s) 44, 60, 152, 1345
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 4 cut(s) 552, 904, 1362, 1648
AlwI GGATC 2 cut(s) 526, 1248
AoxI GGCC 3 cut(s) 235, 604, 876
ApeKI GCWGC 3 cut(s) 313, 1336, 1384
ApoI RAATTY 5 cut(s) 114, 376, 436, 700, 1448
ArsI GACNNNNNNTTYG 6 cut(s) 105, 137, 622, 654, 1280, 1312
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 6 cut(s) 68, 711, 746, 1417, 1493, 1646
BaeGI GKGCMC 1 cut(s) 831
BalI TGGCCA 1 cut(s) 878
BanII GRGCYC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 966
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 3 cut(s) 325, 1323, 1371
BccI CCATC 4 cut(s) 213, 493, 504, 1132
BceAI ACGGC 2 cut(s) 397, 1489
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 3 cut(s) 348, 578, 893
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 4 cut(s) 552, 904, 1362, 1648
BfaI CTAG 1 cut(s) 1687
BfmI CTRYAG 3 cut(s) 1337, 1385, 1458
BfuAI ACCTGC 3 cut(s) 1378, 1389, 1666
BisI GCNGC 3 cut(s) 314, 1337, 1385
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 3 cut(s) 315, 1338, 1386
Bme1390I CCNGG 4 cut(s) 348, 516, 578, 893
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 4 cut(s) 66, 229, 1311, 1326
BmrFI CCNGG 4 cut(s) 348, 516, 578, 893
BmsI GCATC 3 cut(s) 72, 793, 1516
BpiI GAAGAC 1 cut(s) 966
BplI GAGNNNNNCTC 2 cut(s) 1053, 1085
BpmI CTGGAG 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 2 cut(s) 324, 1055
BpuMI CCSGG 1 cut(s) 516
BsaBI GATNNNNATC 1 cut(s) 1146
BsaI GGTCTC 2 cut(s) 904, 1648
BsaJI CCNNGG 4 cut(s) 347, 757, 892, 1551
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1191, 1387
Bse118I RCCGGY 2 cut(s) 250, 1478
Bse1I ACTGG 3 cut(s) 58, 119, 647
Bse8I GATNNNNATC 1 cut(s) 1146
BseBI CCWGG 3 cut(s) 348, 578, 893
BseDI CCNNGG 4 cut(s) 347, 757, 892, 1551
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1191, 1387
BseMII CTCAG 4 cut(s) 269, 548, 791, 1086
BseNI ACTGG 3 cut(s) 58, 119, 647
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 3 cut(s) 325, 1323, 1371
BsgI GTGCAG 2 cut(s) 332, 1374
BshFI GGCC 3 cut(s) 237, 606, 878
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 3 cut(s) 251, 515, 1479
BslFI GGGAC 2 cut(s) 542, 670
BslI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1191, 1387
BsmAI GTCTC 4 cut(s) 552, 904, 1362, 1648
BsmFI GGGAC 2 cut(s) 542, 670
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 3 cut(s) 237, 606, 878
Bso31I GGTCTC 2 cut(s) 904, 1648
Bsp1286I GDGCHC 2 cut(s) 46, 831
Bsp143I GATC 3 cut(s) 518, 1240, 1262
Bsp1720I GCTNAGC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 1551
BspACI CCGC 3 cut(s) 949, 955, 1661
BspANI GGCC 3 cut(s) 237, 606, 878
BspCNI CTCAG 4 cut(s) 268, 547, 790, 1087
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 66, 229, 1311, 1326
BspMAI CTGCAG 2 cut(s) 1341, 1389
BspMI ACCTGC 3 cut(s) 1378, 1389, 1666
BspPI GGATC 2 cut(s) 526, 1248
BspTNI GGTCTC 2 cut(s) 904, 1648
BsrBI CCGCTC 1 cut(s) 949
BsrFI RCCGGY 2 cut(s) 250, 1478
BsrI ACTGG 3 cut(s) 58, 119, 647
BssAI RCCGGY 2 cut(s) 250, 1478
BssECI CCNNGG 4 cut(s) 347, 757, 892, 1551
BssMI GATC 3 cut(s) 518, 1240, 1262
BssT1I CCWWGG 2 cut(s) 757, 1551
Bst2UI CCWGG 3 cut(s) 348, 578, 893
Bst4CI ACNGT 3 cut(s) 965, 1459, 1634
Bst6I CTCTTC 4 cut(s) 889, 902, 1555, 1662
BstAPI GCANNNNNTGC 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 604, 608, 1092
BstDEI CTNAG 5 cut(s) 255, 534, 777, 1095, 1349
BstDSI CCRYGG 1 cut(s) 1551
BstENI CCTNNNNNAGG 1 cut(s) 1385
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 3 cut(s) 521, 1243, 1265
BstMAI GTCTC 4 cut(s) 552, 904, 1362, 1648
BstMBI GATC 3 cut(s) 518, 1240, 1262
BstMWI GCNNNNNNNGC 3 cut(s) 289, 1333, 1342
BstNI CCWGG 3 cut(s) 348, 578, 893
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 4 cut(s) 346, 514, 576, 891
BstSFI CTRYAG 3 cut(s) 1337, 1385, 1458
BstSLI GKGCMC 1 cut(s) 831
BstV1I GCAGC 3 cut(s) 325, 1323, 1371
BstV2I GAAGAC 1 cut(s) 966
BsuRI GGCC 3 cut(s) 237, 606, 878
BtgI CCRYGG 1 cut(s) 1551
BtgZI GCGATG 2 cut(s) 471, 1110
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 1624
BtsIMutI CAGTG 2 cut(s) 654, 1624
BveI ACCTGC 3 cut(s) 1378, 1389, 1666
Cac8I GCNNGC 3 cut(s) 604, 608, 1092
CciI TCATGA 1 cut(s) 165
Cfr10I RCCGGY 2 cut(s) 250, 1478
Cfr13I GGNCC 1 cut(s) 236
CsiI ACCWGGT 1 cut(s) 576
Csp6I GTAC 1 cut(s) 1121
CviAII CATG 6 cut(s) 145, 166, 370, 451, 1402, 1552
CviQI GTAC 1 cut(s) 1121
DdeI CTNAG 5 cut(s) 255, 534, 777, 1095, 1349
DpnI GATC 3 cut(s) 520, 1242, 1264
DpnII GATC 3 cut(s) 518, 1240, 1262
DrdI GACNNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 4 cut(s) 889, 902, 1555, 1662
EarI CTCTTC 4 cut(s) 889, 902, 1555, 1662
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 757, 1551
Eco24I GRGCYC 1 cut(s) 46
Eco31I GGTCTC 2 cut(s) 904, 1648
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 1137
EcoICRI GAGCTC 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 1385
EcoRI GAATTC 1 cut(s) 436
EcoRII CCWGG 3 cut(s) 346, 576, 891
EcoT14I CCWWGG 2 cut(s) 757, 1551
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 2 cut(s) 757, 1551
FaeI CATG 6 cut(s) 148, 169, 373, 454, 1405, 1555
FaqI GGGAC 2 cut(s) 542, 670
FatI CATG 6 cut(s) 144, 165, 369, 450, 1401, 1551
FauI CCCGC 1 cut(s) 948
FauNDI CATATG 1 cut(s) 789
FblI GTMKAC 1 cut(s) 640
Fnu4HI GCNGC 3 cut(s) 314, 1337, 1385
FokI GGATG 1 cut(s) 483
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 3 cut(s) 314, 1337, 1385
FspBI CTAG 1 cut(s) 1687
GluI GCNGC 3 cut(s) 314, 1337, 1385
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 3 cut(s) 237, 606, 878
HapII CCGG 3 cut(s) 251, 515, 1479
Hin1II CATG 6 cut(s) 148, 169, 373, 454, 1405, 1555
HincII GTYRAC 2 cut(s) 616, 1200
HindII GTYRAC 2 cut(s) 616, 1200
HindIII AAGCTT 1 cut(s) 1343
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 3 cut(s) 251, 515, 1479
HphI GGTGA 6 cut(s) 68, 711, 746, 1417, 1493, 1646
Hpy166II GTNNAC 3 cut(s) 616, 641, 1200
Hpy188I TCNGA 3 cut(s) 537, 747, 771
Hpy188III TCNNGA 5 cut(s) 103, 110, 166, 1433, 1581
Hpy8I GTNNAC 3 cut(s) 616, 641, 1200
Hpy99I CGWCG 2 cut(s) 985, 1062
HpyAV CCTTC 5 cut(s) 194, 854, 1183, 1302, 1631
HpyCH4III ACNGT 3 cut(s) 965, 1459, 1634
HpyF10VI GCNNNNNNNGC 3 cut(s) 289, 1333, 1342
HpyF3I CTNAG 5 cut(s) 255, 534, 777, 1095, 1349
Hsp92II CATG 6 cut(s) 148, 169, 373, 454, 1405, 1555
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 3 cut(s) 518, 1240, 1262
LmnI GCTCC 3 cut(s) 70, 1309, 1324
Lsp1109I GCAGC 3 cut(s) 325, 1323, 1371
LweI GCATC 3 cut(s) 72, 793, 1516
MabI ACCWGGT 1 cut(s) 576
MaeI CTAG 1 cut(s) 1687
MaeIII GTNAC 5 cut(s) 3, 689, 733, 1405, 1481
MalI GATC 3 cut(s) 520, 1242, 1264
MbiI CCGCTC 1 cut(s) 949
MboI GATC 3 cut(s) 518, 1240, 1262
MfeI CAATTG 3 cut(s) 92, 326, 706
MhlI GDGCHC 2 cut(s) 46, 831
MlsI TGGCCA 1 cut(s) 878
MluNI TGGCCA 1 cut(s) 878
MmeI TCCRAC 3 cut(s) 60, 1014, 1537
Mox20I TGGCCA 1 cut(s) 878
MscI TGGCCA 1 cut(s) 878
MseI TTAA 4 cut(s) 374, 582, 615, 1533
Msp20I TGGCCA 1 cut(s) 878
MspI CCGG 3 cut(s) 251, 515, 1479
MspR9I CCNGG 4 cut(s) 348, 516, 578, 893
MunI CAATTG 3 cut(s) 92, 326, 706
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 3 cut(s) 348, 578, 893
MwoI GCNNNNNNNGC 3 cut(s) 289, 1333, 1342
NciI CCSGG 1 cut(s) 516
NcoI CCATGG 1 cut(s) 1551
NdeI CATATG 1 cut(s) 789
NdeII GATC 3 cut(s) 518, 1240, 1262
NlaIII CATG 6 cut(s) 148, 169, 373, 454, 1405, 1555
NlaIV GGNNCC 4 cut(s) 66, 229, 1311, 1326
NmuCI GTSAC 2 cut(s) 1405, 1481
NspI RCATGY 1 cut(s) 373
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 2 cut(s) 1378, 1389
PciI ACATGT 1 cut(s) 369
PctI GAATGC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 403
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 3 cut(s) 315, 1338, 1386
PscI ACATGT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 1569
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 3 cut(s) 346, 576, 891
PspGI CCWGG 3 cut(s) 346, 576, 891
PspN4I GGNNCC 4 cut(s) 66, 229, 1311, 1326
PspPI GGNCC 1 cut(s) 236
PstI CTGCAG 2 cut(s) 1341, 1389
RsaI GTAC 1 cut(s) 1122
RsaNI GTAC 1 cut(s) 1121
SacI GAGCTC 1 cut(s) 46
SaqAI TTAA 4 cut(s) 374, 582, 615, 1533
SatI GCNGC 3 cut(s) 314, 1337, 1385
Sau3AI GATC 3 cut(s) 518, 1240, 1262
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 4 cut(s) 348, 516, 578, 893
SduI GDGCHC 2 cut(s) 46, 831
SexAI ACCWGGT 1 cut(s) 576
SfaNI GCATC 3 cut(s) 72, 793, 1516
SfcI CTRYAG 3 cut(s) 1337, 1385, 1458
SmlI CTYRAG 2 cut(s) 339, 1070
SmoI CTYRAG 2 cut(s) 339, 1070
SsiI CCGC 3 cut(s) 949, 955, 1661
SspI AATATT 1 cut(s) 357
SspMI CTAG 1 cut(s) 1687
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 4 cut(s) 346, 514, 576, 891
StyI CCWWGG 2 cut(s) 757, 1551
TaaI ACNGT 3 cut(s) 965, 1459, 1634
TaqI TCGA 6 cut(s) 111, 983, 1038, 1057, 1299, 1599
Tru1I TTAA 4 cut(s) 374, 582, 615, 1533
Tru9I TTAA 4 cut(s) 374, 582, 615, 1533
TscAI CASTG 2 cut(s) 654, 1624
TseFI GTSAC 2 cut(s) 1405, 1481
TseI GCWGC 3 cut(s) 313, 1336, 1384
Tsp45I GTSAC 2 cut(s) 1405, 1481
TspDTI ATGAA 9 cut(s) 154, 182, 407, 467, 806, 867, 1188, 1390, 1638
TspGWI ACGGA 3 cut(s) 220, 947, 1500
TspRI CASTG 2 cut(s) 654, 1624
Van91I CCANNNNNTGG 1 cut(s) 403
XagI CCTNNNNNAGG 1 cut(s) 1385
XapI RAATTY 5 cut(s) 114, 376, 436, 700, 1448
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.